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Effect of co-feeding on biological performance and biochemical composition of meagre (Argyrosomus Regius Asso, 1801) larvae

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Effect of co-feeding on biological performance and biochemical composition of meagre (Argyrosomus Regius Asso, 1801) larvae

Author: Fernandez-Palacios, H.,Hernandez-Cruz, C.M,Schuchardt, Dominique,Izquierdo, Marisol,Roo, Javier
Year: 2009
Source: https://accedacris.ulpgc.es/jspui/bitstream/10553/6133/4/0628617_00000_0000.pdf
E ec o co- eeding on biological pe o mance and
biochemical composi ion o meag e (A gy osomus
egius Asso, 1801) la ae
H. FERNÁNDEZ-PALACIOS, C. M. HERNÁNDEZ-CRUZ, D. SCHUCHARDT, M. S. IZQUIERDO AND F. J. ROO
G upo de In es igación en Acuicul u a (GIA).P.O. Box 56. 35200 Telde, Cana y Islands. Spain.
Abs ac
Acknowledgemen
Ma e ials and me hods
Meag e, A gy osomus egious A., is a new species o aquacul u e di e si ica ion. Di e en
zoo- echnical pa ame e s such as eeding sequence and ea ing p o ocols du ing he la al
phase need o be op imized. Eggs o meag e we e cul u ed unde in ensi e sys em (50
ind .l-1 in 2m3 anks) o e alua e he e ec o he di e en co- eeding egimes (5,10,15
and 20 days o co- eeding A emia+MD om 10dah) o e g ow h su i al, s ess esis ance
and biochemical composi ion o he la ae. A 40dah, la al su i al anged om 13.11-
22.33% o la ae ed di ec ly om o i e s o MD (12dah) o la ae co- ed A emia and
mic odie o 20days. No signi ican di e ences we e ound on la al d y body weigh ,
howe e s anda d leng h a ies om (14.87-18.53) depending on he co- eeding egime,
being highe in he la ae wi h longe co eeding pe iods. Su i al a e s ess esis ance
es anged om 53.3±11.5% and 75.0± 13.8%.
• 15 ibe glass anks wi h a capaci y o 150 l.
• The expe imen was conduc ed in iplica e
• sea wa e was p e iously il e ed and s e ilized by UV adia ion.
• Tª: 20.7 ± 0.8 º C
• O2: 5.85 ± 0.12 ppm
• The pho ope iod was 12-12, ligh and da k, wi h a i icial ligh .
• Ini ial s ocking densi y was o 50 la ae.l-1 wi h 9 days a e ha ching
la ae (LS = 4.24 ± 0.21mm, PS = 0.13 ± 0.008 mg) s ocking was
pe o med acco ding o he olume ic me hod desc ibed by Bo e o
(2008).
S a is ically di e ences we e ound in s anda d leng h, showing ha he la ae wi h a co- eeding ime o 10, 15
and 20 days we e signi ican ly bigge han hose o 0 and 5 days co- eeding. Al es e al. (2006) wo king wi h a
snook (Cen opomus pa allelus) la ae o 30 days o li e and wi h a co- eeding pe iod o 5, 10 and 15 days ound
signi ican di e ences in he s anda d leng h.
No s a is ically signi ican di e ences we e ound in inal la ae su i al. Rega ding he ac i i y es o ai esis ance
no signi ican di e ences be ween ea men s we e ound, al hough la ge sizes la ae show a be e su i al.
Simila esul s we e obse ed by Liu e al. (2002) wo king wi h o sea b eam (Spa us au a a) la ae.
•S anda leng h (n=30 pe ank; 40dah).
•D y weigh (n=30 pe ank; 40dah).
•Final su i al (40 dah).
•Ac i i y es (n = 30 pe ank; 120 seconds ai exposu e a 40dah).
•Biochemical analysis
Resul s and discussion
This wo k was done unde he p ojec PLANACOR
unded by La Jun a Aseso a Nacional de Cul i os
Ma inos (JACUMAR).
Expe imen al condi ions Feed
De e mina ions
Ini ial S ocking
The e we e no s a is ically signi ican di e ences in any o he biochemical composi ion o he la ae, his could
p obably be due o he eed used, and ha was he same o all ea men s, a ying only on he du a ion o i s
use. Simila esul s we e shown by Bo e o (2008) in an expe imen wi h la ae o he same species.
Expe imen al anks.
Figu e 1. Range o he calcula ed pa ame e s in he
expe imen .
Figu e 2. Range o he calcula ed pa ame e s in he
expe imen .
Table I. Feed sequence and quan i ies used.
Table II. Biochemical composi ion o he expe imen al la ae.
T ea men 0 5 10 15 20 Feed added
Days a e ha ching
Phy oplanc on 9-15 9-15 9-15 9-15 9-15 300.000 cel.ml-1
Ro i e 9-15 9-15 9-15 9-15 9-15 6-7ml-1
Nauplii 12 12 12 12 0.5ml-1
Me aNauplii 13 13 13 13 0.5ml-1 + 0.5ml-
1
Me anauplii 14 14 14 14 1 ml-1
Me anauplii 15-17 15-22 15-27 15-32 1.5ml-1
Mic o-die GM
150-300 (50%) 12-30 12-30 12-30 12-30 12-30 15%
biomass.d-1
Mic o-die GM
300 (100%) 30-40 30-40 30-40 30-40 30-40 15%
biomass.d-1
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T e . P o ein Lipìds ARA EPA DHA n-3 HUFA
0 10.16±0,25 2.06±0.05 1.63±0.05 8.75±0.11 16.31±0.76 27.40±0.51
5 10.31±0.14 2.08±0.04 1.63±0.06 8.35±0.26 16.54±0.36 27.42±0.76
10 10.19±0.17 2.16±0.06 1.76±0.06 8.72±0.41 16.30±0.91 27.50±0.48
15 10.43±0.19 2.10±0.05 1,75±0.05 8.53±0.25 16.09±0.53 26.72±0.39
20 10.14±0.33 2.08±0.06 1.69±0.07 8.65±0.17 16.34±0.22 26.89±0.63