Effect of co-feeding on biological performance and biochemical composition of meagre (Argyrosomus Regius Asso, 1801) larvae

E ec o co- eeding on biological pe o mance and biochemical composi ion o meag e (A gy osomus egius Asso, 1801) la ae H. FERNÁNDEZ-PALACIOS, C. M. HERNÁNDEZ-CRUZ, D. SCHUCHARDT, M. S. IZQUIERDO AND F. J. ROO G upo de In es igación en Acuicul u a (GIA).P.O. Box 56. 35200 Telde, Cana y Islands. Spain. Abs ac Acknowledgemen Ma e ials and me hods Meag e, A gy osomus egious A., is a new species o aquacul u e di e si ica ion. Di e en zoo- echnical pa ame e s such as eeding sequence and ea ing p o ocols du ing he la al phase need o be op imized. Eggs o meag e we e cul u ed unde in ensi e sys em (50 ind .l-1 in 2m3 anks) o e alua e he e ec o he di e en co- eeding egimes (5,10,15 and 20 days o co- eeding A emia+MD om 10dah) o e g ow h su i al, s ess esis ance and biochemical composi ion o he la ae. A 40dah, la al su i al anged om 13.11- 22.33% o la ae ed di ec ly om o i e s o MD (12dah) o la ae co- ed A emia and mic odie o 20days. No signi ican di e ences we e ound on la al d y body weigh , howe e s anda d leng h a ies om (14.87-18.53) depending on he co- eeding egime, being highe in he la ae wi h longe co eeding pe iods. Su i al a e s ess esis ance es anged om 53.3±11.5% and 75.0± 13.8%. • 15 ibe glass anks wi h a capaci y o 150 l. • The expe imen was conduc ed in iplica e • sea wa e was p e iously il e ed and s e ilized by UV adia ion. • Tª: 20.7 ± 0.8 º C • O2: 5.85 ± 0.12 ppm • The pho ope iod was 12-12, ligh and da k, wi h a i icial ligh . • Ini ial s ocking densi y was o 50 la ae.l-1 wi h 9 days a e ha ching la ae (LS = 4.24 ± 0.21mm, PS = 0.13 ± 0.008 mg) s ocking was pe o med acco ding o he olume ic me hod desc ibed by Bo e o (2008). S a is ically di e ences we e ound in s anda d leng h, showing ha he la ae wi h a co- eeding ime o 10, 15 and 20 days we e signi ican ly bigge han hose o 0 and 5 days co- eeding. Al es e al. (2006) wo king wi h a snook (Cen opomus pa allelus) la ae o 30 days o li e and wi h a co- eeding pe iod o 5, 10 and 15 days ound signi ican di e ences in he s anda d leng h. No s a is ically signi ican di e ences we e ound in inal la ae su i al. Rega ding he ac i i y es o ai esis ance no signi ican di e ences be ween ea men s we e ound, al hough la ge sizes la ae show a be e su i al. Simila esul s we e obse ed by Liu e al. (2002) wo king wi h o sea b eam (Spa us au a a) la ae. •S anda leng h (n=30 pe ank; 40dah). •D y weigh (n=30 pe ank; 40dah). •Final su i al (40 dah). •Ac i i y es (n = 30 pe ank; 120 seconds ai exposu e a 40dah). •Biochemical analysis Resul s and discussion This wo k was done unde he p ojec PLANACOR unded by La Jun a Aseso a Nacional de Cul i os Ma inos (JACUMAR). Expe imen al condi ions Feed De e mina ions Ini ial S ocking The e we e no s a is ically signi ican di e ences in any o he biochemical composi ion o he la ae, his could p obably be due o he eed used, and ha was he same o all ea men s, a ying only on he du a ion o i s use. Simila esul s we e shown by Bo e o (2008) in an expe imen wi h la ae o he same species. Expe imen al anks. Figu e 1. Range o he calcula ed pa ame e s in he expe imen . Figu e 2. Range o he calcula ed pa ame e s in he expe imen . Table I. Feed sequence and quan i ies used. Table II. Biochemical composi ion o he expe imen al la ae. T ea men 0 5 10 15 20 Feed added Days a e ha ching Phy oplanc on 9-15 9-15 9-15 9-15 9-15 300.000 cel.ml-1 Ro i e 9-15 9-15 9-15 9-15 9-15 6-7ml-1 Nauplii 12 12 12 12 0.5ml-1 Me aNauplii 13 13 13 13 0.5ml-1 + 0.5ml- 1 Me anauplii 14 14 14 14 1 ml-1 Me anauplii 15-17 15-22 15-27 15-32 1.5ml-1 Mic o-die GM 150-300 (50%) 12-30 12-30 12-30 12-30 12-30 15% biomass.d-1 Mic o-die GM 300 (100%) 30-40 30-40 30-40 30-40 30-40 15% biomass.d-1                                T e . P o ein Lipìds ARA EPA DHA n-3 HUFA 0 10.16±0,25 2.06±0.05 1.63±0.05 8.75±0.11 16.31±0.76 27.40±0.51 5 10.31±0.14 2.08±0.04 1.63±0.06 8.35±0.26 16.54±0.36 27.42±0.76 10 10.19±0.17 2.16±0.06 1.76±0.06 8.72±0.41 16.30±0.91 27.50±0.48 15 10.43±0.19 2.10±0.05 1,75±0.05 8.53±0.25 16.09±0.53 26.72±0.39 20 10.14±0.33 2.08±0.06 1.69±0.07 8.65±0.17 16.34±0.22 26.89±0.63