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BMC Pha macology
Open Access
Mee ing abs ac
Ni a e ole ance-induced de e io a ion o he ischemic
adap abili y o he hea
Zol án Szil ássy*1, János Szaszkó2, Róbe Döb ön e3, Józse Néme h2,
Réka Zs Sá i2, Csaba Pankucsi2, Angelika Va ga2, Józse Tőzsé 4, László Fésüs4
and Ba na Pei l1
Add ess: 1CERAMED Resea ch G oup, Uni e si y Deb ecen, Hunga y, 2Depa men o Pha macology and Pha maco he apy, Uni e si y o
Deb ecen, Hunga y, 3Depa men o Anes hesiology and ICU, Pe z Aladá Coun y Hospi al, Győ , Hunga y and 4Depa men o Biochemis y and
Molecula Biology, Uni e si y o Deb ecen, Hunga y
Email: Zol án Szil ássy* - szil [email protected] macol.do e.hu
* Co esponding au ho
In oduc ion
We es ed whe he pos condi ioning limi ed in a c size in
abbi s wi h haemodynamic ni a e ole ance.
Me hods
Male abbi s made ole an o he hypo ensi e esponse o
30 μg/kg in a enous ni oglyce in (NG) by a p eceding
one-week exposu e o ansde mal NG (0.07 mg/kg/h)
we e subjec ed o 35 min co ona y occlusion ( es
ischemia) ollowed by 3 h o epe usion wi h he ollow-
ing addi ional in e en ions: no in e en ion (NI); pos -
condi ioning pacing (PPC): i e cycles o 5 min pe iods o
apid en icula pacing (500 b.p.m.), o pos condi ion-
ing co ona y occlusion (PCO): i e cycles o 5 min co o-
na y occlusion wi h 10 min in e pacing/in e occlusion
in e als, applied a e he end o he es ischemia. These
p o ocols we e applied in bo h ni a e- ole an and non-
ole an animals. In a c size exp essed as a pe cen age o
a ea a isk (I/R) was de e mined by iphenyl e azolium
chlo ide s aining, le en icula cyclic nucleo ides we e
de e mined by adioimmunoassay om samples ou o
he a ea a isk, 75 min a e he es ischemia.
Resul s
In non- ole an animals bo h PPC and PCO educed he
I/R compa ed o he NI g oup. When animals had been
made ni a e- ole an , he I/R was signi ican ly highe in
he NI g oup compa ed wi h non- ole an animals and
he bene icial e ec o he PPC o PCO on he I/R disap-
pea ed.
Conclusion
We conclude ha (i) ni a e ole ance blocks pos condi-
ioning induced by ei he PPC o PCO, (ii) PPC is mo e
e ec i e pos -condi ioning challenge han PCO, and (iii)
ni a e ole ance pe se educes he capabili y o he hea
o ole a e an ischemic insul .
om 13 h Scien i ic Symposium o he Aus ian Pha macological Socie y (APHAR). Join Mee ing wi h he Aus ian Socie y o Toxicology (ASTOX) and he
Hunga ian Socie y o Expe imen al and Clinical Pha macology (MFT)
Vienna, Aus ia. 22–24 No embe 2007
Published: 14 No embe 2007
BMC Pha macology 2007, 7(Suppl 2):A49 doi:10.1186/1471-2210-7-S2-A49
<supplemen > < i le> <p>13 h Scien i ic Symposium o he Aus ian Pha macological Socie y (APHAR). Join Mee ing wi h he Aus ian Socie y o Toxicology (ASTOX) and he Hunga ian Socie y o Expe imen al and Clinical Pha macology (MFT)</p> </ i le> <edi o >E ns Singe and Thomas G iesbache </edi o > <no e>Mee ing abs ac s – A single PDF con aining all abs ac s in his Supplemen is a ailable <a h e ="[h p://www.biomedcen al.com/con en / iles/pd /1471-2210-7-S2- ull.pd ]">he e</a></no e> <u l>h p://www.biomedcen al.com/con en /pd /1471-2210-7-S2-in o.pd </u l> </supplemen >
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