Nitrate tolerance-induced deterioration of the ischemic adaptability of the heart

BioMed Cen al Page 1 o 1 (page numbe no o ci a ion pu poses) BMC Pha macology Open Access Mee ing abs ac Ni a e ole ance-induced de e io a ion o he ischemic adap abili y o he hea Zol án Szil ássy*1, János Szaszkó2, Róbe Döb ön e3, Józse Néme h2, Réka Zs Sá i2, Csaba Pankucsi2, Angelika Va ga2, Józse Tőzsé 4, László Fésüs4 and Ba na Pei l1 Add ess: 1CERAMED Resea ch G oup, Uni e si y Deb ecen, Hunga y, 2Depa men o Pha macology and Pha maco he apy, Uni e si y o Deb ecen, Hunga y, 3Depa men o Anes hesiology and ICU, Pe z Aladá Coun y Hospi al, Győ , Hunga y and 4Depa men o Biochemis y and Molecula Biology, Uni e si y o Deb ecen, Hunga y Email: Zol án Szil ássy* - szil [email protected] macol.do e.hu * Co esponding au ho In oduc ion We es ed whe he pos condi ioning limi ed in a c size in abbi s wi h haemodynamic ni a e ole ance. Me hods Male abbi s made ole an o he hypo ensi e esponse o 30 μg/kg in a enous ni oglyce in (NG) by a p eceding one-week exposu e o ansde mal NG (0.07 mg/kg/h) we e subjec ed o 35 min co ona y occlusion ( es ischemia) ollowed by 3 h o epe usion wi h he ollow- ing addi ional in e en ions: no in e en ion (NI); pos - condi ioning pacing (PPC): i e cycles o 5 min pe iods o apid en icula pacing (500 b.p.m.), o pos condi ion- ing co ona y occlusion (PCO): i e cycles o 5 min co o- na y occlusion wi h 10 min in e pacing/in e occlusion in e als, applied a e he end o he es ischemia. These p o ocols we e applied in bo h ni a e- ole an and non- ole an animals. In a c size exp essed as a pe cen age o a ea a isk (I/R) was de e mined by iphenyl e azolium chlo ide s aining, le en icula cyclic nucleo ides we e de e mined by adioimmunoassay om samples ou o he a ea a isk, 75 min a e he es ischemia. Resul s In non- ole an animals bo h PPC and PCO educed he I/R compa ed o he NI g oup. When animals had been made ni a e- ole an , he I/R was signi ican ly highe in he NI g oup compa ed wi h non- ole an animals and he bene icial e ec o he PPC o PCO on he I/R disap- pea ed. Conclusion We conclude ha (i) ni a e ole ance blocks pos condi- ioning induced by ei he PPC o PCO, (ii) PPC is mo e e ec i e pos -condi ioning challenge han PCO, and (iii) ni a e ole ance pe se educes he capabili y o he hea o ole a e an ischemic insul . om 13 h Scien i ic Symposium o he Aus ian Pha macological Socie y (APHAR). Join Mee ing wi h he Aus ian Socie y o Toxicology (ASTOX) and he Hunga ian Socie y o Expe imen al and Clinical Pha macology (MFT) Vienna, Aus ia. 22–24 No embe 2007 Published: 14 No embe 2007 BMC Pha macology 2007, 7(Suppl 2):A49 doi:10.1186/1471-2210-7-S2-A49 <supplemen > < i le> <p>13 h Scien i ic Symposium o he Aus ian Pha macological Socie y (APHAR). Join Mee ing wi h he Aus ian Socie y o Toxicology (ASTOX) and he Hunga ian Socie y o Expe imen al and Clinical Pha macology (MFT)</p> </ i le> <edi o >E ns Singe and Thomas G iesbache </edi o > <no e>Mee ing abs ac s – A single PDF con aining all abs ac s in his Supplemen is a ailable <a h e ="[h p://www.biomedcen al.com/con en / iles/pd /1471-2210-7-S2- ull.pd ]">he e</a></no e> <u l>h p://www.biomedcen al.com/con en /pd /1471-2210-7-S2-in o.pd </u l> </supplemen > This abs ac is a ailable om: h p://www.biomedcen al.com/1471-2210/7/S2/A49 © 2007 Szil ássy e al; licensee BioMed Cen al L d.