HYPERSPECTRAL POLARIMETRIC IMAGING FOR MULTISCALE
LABEL-FREE CHARACTERIZATION AND FUNCTIONAL
DIAGNOSTICS OF BIOTISSUES
Alexande BYKOV1,* Oleksii SIERYI1, I an LOPUSHENKO1, An on SDOBNOV1,
Vik o DREMIN2, Igo MEGLINSKI2
1 Op o-Elec onics and Measu emen Techniques, Uni e si y o Oulu; Oulu, Finland;
2 As on Ins i u e o Pho onic Technologies, As on Uni e si y, Bi mingham, UK
* Co espondence: alexande .byko @oulu. i
Keywo ds: pola iza ion imaging, hype spec al imaging, bio issue diagnos ics
Bulk issue pola ime y exploi s he ec o na u e o ligh o p o ide in insic con as based on issue
mic os uc u e, while hype spec al imaging e eals unc ional and biochemical in o ma ion.
Combining hese wo modali ies enables a mul idimensional cha ac e iza ion o bio issues a di e en
spa ial scales, p o iding a e sa ile ool o unc ional imaging o in i o issues [1] as well as label-
ee, non-des uc i e cha ac e iza ion o his ological issue blocks [2], wi h he po en ial o apid
au oma ed segmen a ion o malignan egions.
Two ypes o sys ems ha e been implemen ed and compa ed. The i s one is based on ull S okes
pola ime y. This sys em u ilizes a supe con inuum ibe lase coupled wi h an acous o-op ic unable
il e o selec p obing wa eleng hs be ween 450-650 nm wi h 5 nm accu acy. Illumina ion is igh
ci cula ly pola ized ia a qua e -wa e pla e and ocused on he sample using a 45 mm lens. Di usely
e lec ed ligh is collec ed by a 100× objec i e, passed h ough a 100 μm pinhole o spa ial il e ing,
and analyzed by a pola ime e p o iding S okes pa ame e s a each pixel and wa eleng h. This
con igu a ion achie es a spa ial esolu ion be e han 5 μm and enables scanning o a eas anging
om cm o μm scales, app oaching single-cell-le el esolu ion. Op imal sou ce-de ec o sepa a ions o
60-100 μm we e iden i ied: smalle sepa a ions dec eased cance sensi i i y, while la ge ones
inc eased depola iza ion noise. Speci ic expe imen al esul s demons a ed ha he sys em could
clea ly dema ca e cance ous egions wi hin o malin- ixed pa a in-embedded b eas issue blocks.
The ob ained deg ee o pola iza ion maps showed high con as be ween cance ous, ib o ic, and
adipose issue a eas, wi h s ong co ela ion o s anda d his opa hological g ound u h. The second
sys em is a compac handheld assembly inco po a ing a hype spec al came a based on a mic o unable
Fab y-Pe o il e . I achie es a spec al esolu ion o 6-10 nm ac oss he 510-900 nm ange, u ilizing
b oadband illumina ion om a halogen lamp deli e ed ia a ib e-op ic ing illumina o . Hype spec al
images we e cap u ed o e an a ea o 8 × 8 cm² wi h a megapixel esolu ion. Fo pola iza ion
measu emen s, a o a ing linea pola ize enabled acquisi ion o co-pola ized and c oss-pola ized
images, om which he Deg ee o Linea Pola iza ion (DOLP) spec a we e calcula ed in each spa ial
pixel. In i o skin measu emen s, as well as Mon e Ca lo simula ions inco po a ing a mul ilaye ed
skin model wi h ealis ic op ical p ope ies, demons a ed ha issue sca e ing changes had a g ea e
impac on pola iza ion al e a ions han abso p ion a ia ions, con i ming he sensi i i y o he DOLP
o mic os uc u al changes, such as collagen ib e densi y and o ganiza ion. In conclusion, he
in eg a ion o hype spec al imaging and pola ime y p o ides a powe ul app oach o label- ee, non-
in asi e unc ional imaging o biological issues. Full S okes pola ime y excels in mul iscale
s uc u al cha ac e iza ion o his ological issue blocks wi h high spa ial esolu ion, while came a-
based hype spec al pola iza ion imaging is be e sui ed o in i o assessmen o issue s uc u e and
unc ion due o i s compac ness, obus ness, and apid da a p ocessing. Combined, hese
complemen a y app oaches ha e he po en ial o ad ance diagnos ic his opa hology and unc ional
issue imaging owa ds au oma ed, apid, and objec i e assessmen s in clinical wo k lows.
Acknowledgemen s: This wo k has been suppo ed by he Eu opean Union’s Ho izon Eu ope esea ch
and inno a ion p og amme unde g an ag eemen No 101185769.
[1]. V. D emin, E. Zha kikh, I. Lopushenko, e al., J. Biomed. Op ., 29(5), 052912 (2024).
[2]. M. Bo o ko a, O. Sie yi, I. Lopushenko, e al., IEEE T ans. Med. Imaging., 41(4), 977-982 (2022).
