Ipomoea bu iliae (Con ol ulaceae), a new species o mo ning
glo y disco e ed in he Bo bo ema Pla eau, no heas e n B azil
Belo D.P.1,2,3,4*, San os E.A.V.1,3 & E.C. Gaspa ino4
1P og ama de Pós-G aduação em Biodi e sidade, Depa amen o de Biologia, Uni e sidade Fede al Ru al de Pe nambuco, 52171-
900, Reci e, Pe nambuco, B azil
2Labo a ó io de Sis emá ica In eg a i a, Depa amen o de Biologia, Uni e sidade Fede al Ru al de Pe nambuco, 52171-900, Reci e,
Pe nambuco, B azil
3Labo a ó io de Ana omia Vege al, Depa amen o de Bo ânica, Cen o de Biociências, Uni e sidade Fede al de Pe nambuco,
50740-570, Reci e, Pe nambuco, B azil
4Labo a ó io de Mo ologia Vege al e Palinologia, Depa amen o de Biologia, Faculdade de Ciências Ag á ias e Ve e iná ias,
Uni e sidade Es adual Paulis a, 14884-900, Jabo icabal, São Paulo, B azil
*E-mail: [email p o ec ed]
Abs ac : Ipomoea bu iliae Belo (Con ol ulaceae) is
desc ibed and illus a ed as a new species. We p o ide a
axonomic ea men along wi h ana omical, cy ogene ic,
and palynological desc ip ions o his new species.
Addi ionally, we include illus a ions, pho og aphs,
and de ails abou i s geog aphical dis ibu ion and
conse a ion s a us. A compa a i e lis highligh s key
mo phological cha ac e s o I. bu iliae in ela ion o closely
ela ed species. I sha es some mo phological simila i ies
wi h I. pa asi ica (Kun h) G.Don. Howe e , I. bu iliae is
dis inguished by i s whi e co olla, spiny p ojec ions on
he s em, pe iole, and peduncle, as well as a campanula e
co olla and ec o ichomes along he ilamen s.
Keywo ds: B azilian lo a, CMA/DAPI banding,
Endemism, Lea ana omy, Palynology, Taxonomy
o he amily in he coun y has expanded wi h he
publica ion o se e al new species (e.g., Wood e
al., 2017; San os e al., 2019, 2020a, 2020b, 2021;
Nepomuceno e al., 2022; Belo e al., 2023a, 2024;
Pas o e e al., 2023; San os & Bu il, 2024).
Ipomoea L. is he mos di e se genus o
Con ol ulaceae, comp ising app oxima ely 800
species (Wood e al., 2020; Delgado-Junio e al.,
2023). Despi e i s mo phological a iabili y, he
genus is easily ecognized by se e al key ai s:
echina e and pan opo a e pollen g ains, an en i e
s yle, a s igma usually wi h wo globose s igma ic
lobes, and 4- al ed capsules (Aus in & Ca alcan e,
1982; S e ano ić e al., 2002). The genus has abou
160 species in B azil, o which 65 a e endemic
(San os e al., 2021; Simão-Bianchini e al., 2024).
Rep esen a i es o he genus a e cha ac e ized by
ha ing a climbing, he baceous, o sh ubby habi ,
simple o compound al e na e lea es, absen s ipules,
cymose in lo escences, unnel o m, campanula e,
o hypoc a e i o m co olla (Aus in, 2004; Fe ei a
& Mio o, 2009; Delgado-Junio e al., 2023). The
shape, p opo ion, indumen um, and o namen a ion
o he sepals a e essen ial axonomic cha ac e s
o delimi a ion o species o he genus, especially
when compa ed wi h ege a i e cha ac e s (Wood
e al., 2020; Delgado-Junio e al., 2023).
Recei ed: 28.10.2024; Re ised & Accep ed: 08.12.2024
Published Online: 31.12.2024
In oduc ion
Con ol ulaceae comp ises abou 60 gene a and
1,900 species, wi h a cosmopoli an dis ibu ion
p edominan ly ound in opical egions (S aples
& B ummi , 2007; POWO, 2024). In B azil, he
amily is ep esen ed by 24 gene a and a ound
430 species (including one endemic genus and
app oxima ely 200 endemic species), dis ibu ed
ac oss all phy ogeog aphic domains (Simão-
Bianchini, 2024). In ecen yea s, he knowledge
Vol. 34(5): 488–505 (2024)
ISSN: 0971-2313 (P in edi ion)
ISSN: 2582-2438 (Online edi ion)
h ps://dx.doi.o g/10.22244/ heedea.2024.34.05.10
Rheedea
Jou nal o he Indian Associa ion o Angiospe m Taxonomy
RESEARCH ARTICLE
489
Belo e al.
Ana omical, palynological, and cy ogene ic s udies
ha e been used o suppo he mo phological
delimi a ions o species in se e al plan g oups, in
addi ion o being used o desc ibe new species (e.g.,
Smi h & Smi h, 1942; Gomes e al., 2005; Almeida
e al., 2016; San os e al., 2019, 2020a, 2020b;
Nepomuceno e al., 2022; Belo e al., 2023a, 2023b,
2023c, 2024). In Con ol ulaceae, ana omical
cha ac e s such as he shape o he pe iole, mid ib,
and mesophyll a e in o ma i e o delimi ing
species o A gy eia Lou . (T aipe m e al., 2017),
Camonea Ra . (San os e al., 2024), Daus inia Bu il
& A.R. Simões (Alenca e al., 2024), E ol ulus L.
(Ke ja un e al., 2016; San os e al., 2020a), and
Jacquemon ia Choisy (Belo e al. 2023b, 2023c, 2024).
Du ing ield expedi ions in he No heas
egion o B azil, we encoun e ed an un amilia
mo pho ype o Ipomoea, which we s udied in
de ail. One popula ion o his mo pho ype was
iden i ied, and he e we desc ibe i as a new Ipomoea
species, suppo ed by mo phological, ana omical,
palynological, and cy ogene ic analyses.
Ma e ials and Me hods
S udy a ea
Field expedi ions we e ca ied ou in no heas e n
B azil, be ween May 2023 and July 2024. The
desc ip ion o he new species was based on
specimens collec ed om one popula ion in he
A eia municipali y, Pa aíba S a e, B azil (Fig.
1). This a ea is pa o he Bo bo ema Pla eau
(BP), one o he eco egions wi hin he Caa inga
phy ogeog aphic domain. The BP spans an a ea o
43,460 km², equi alen o 2.61% o No heas e n
B azil (Sil a e al., 2003). I ex ends ac oss he
Fig. 1.
Dis ibu ion o Ipomoea bu iliae Belo, sp. no . in B azil.
490
A new species o mo ning glo y o B azilian lo a
s a es o Alagoas, Pe nambuco, Pa aíba, and Rio
G ande do No e (Giulie i e al., 2004), wi h
al i udes anging om 500 o 1200 m (Sil a e al.,
2003; Mo ais Ne o e al. 2009). The p edominan
ege a ion in he BP is hypoxe ophilous Caa inga
(Sil a e al., 2003).
Mo phology and conse a ion s a us
Specimens o mo phologically closely ela ed species
we e analyzed om he ollowing he ba ia: EAC,
EAN, HCES, IPA, PEUFR, and UFP (ac onyms
ollow Thie s, 2024). The axonomic desc ip ion
and e minology ollow Meisne (1869), Wood
e al. (2020), Ha is and Ha is (2001), and Ellis
e al. (2009). A p elimina y conse a ion s a us
assessmen was pe o med based on he IUCN Red
Lis Ca ego ies and C i e ia (IUCN, 2012, 2024).
The occu ence map was gene a ed using QGIS
so wa e 3.22 Białowieża (h ps://qgis.o g/).
Ana omical analyses o lea es
Fo compa a i e ana omical analyses, h ee lea es
om he hi d node o he s em we e ob ained om
i e specimens o he new species and o I. pa asi ica
(Kun h) G.Don in he ield (Table 1). These lea es
we e ixed in FAA 50 ( o maldehyde, ace ic acid,
and 50% e hanol) o 48 hou s (Johansen, 1940)
and subsequen ly s o ed in 70% e hanol. F eehand
sec ions we e made om he median egion o
he lea blade, pe iole, and s em. These sec ions
we e clea ed and s ained wi h sa anin-as a
blue (Buka sch, 1972). The slides we e p epa ed
ollowing he p o ocols o K aus and A duin (1997),
analyzed using a Leica DM500 pho omic oscope,
and deposi ed in he Labo a o y o In eg a i e
Sys ema ics a he Fede al Ru al Uni e si y o
Pe nambuco, in Reci e, B azil. Samples close o
he lea ma gins we e dehyd a ed and p epa ed o
scanning elec on mic oscopy (SEM) analysis using
a Hi achi SEM, model TM4000 Plus. SEM images
we e p ocessed using Co elDRAW® 2021 so wa e.
Palynological analyses
Fo pollen mo phology analyses, a leas i e
buds we e nea ing an hesis collec ed om h ee
specimens pe popula ion o ob ain a signi ican
Table 1. Specimens used in mic omo phological analyses.
Taxa Locali y Vouche He ba i-
um
Ana-
omical
analysis
Palyno-
logical
analysis
Cy oge-
ne ic
analysis
Geog aphic
coo dina es
(la i ude,
longi ude)
Ipomoea bu iliae
Belo, sp. no .
A eia, Fede al
Uni e si y o
Pa aíba, Pa aíba
s a e – B azil
D. Belo &E.
Ba bie 740
PEUFR × × × 6° 58’ 10” S,
35° 42’ 50” W
Ipomoea alba L. A eia, Campus
CCA-UFPB,
Pa aíba s a e –
B azil
L.P. Félix &
J.P. Dan as
5000
EAN – × – 6° 58’ 08” S,
35° 43’ 01” W
Ipomoea ma cellia
Meisn.
Remígio, Pa aíba
s a e – B azil
L.P. Félixe
al. 10756
EAN – × – 6° 57’ 32” S,
35° 46’ 55” W
Ipomoea pa asi ica
(Kun h) G.Don
Cui é, Pa aíba
s a e – B azil
V. F. Souza
s.n.
HCES
722
× × – 6° 29’ 36” S,
36° 09’ 24” W
Ipomoea pa asi ica
(Kun h) G.Don
Ubaja a, Cea á
s a e – B azil
J.A.A.M
Lou ençoe
al. 98
PEUFR × × – 3° 50’ 43” S,
40° 54’ 24” W
491
Belo e al.
Table 2. Compa ison among Ipomoea bu iliae Belo, sp. no . (Con ol ulaceae) and h ee mo phologically
simila species.
Cha ac e Ipomoea bu iliae
Belo, sp. no .
Ipomoea alba L. Ipomoea ma cellia
Meisn.
Ipomoea pa asi ica
(Kun h) G.Don
S ems wi h spiny
p ojec ions
P esen P esen Absen P esen
Lea indumen um Pubescen Glab ous Velu inous o
lana e
Se iceous
Lea apex Cauda e Acumina e Acu e Acu e
Ou e sepal shape O bicula Lanceola e O a e o oblong Ellip ic
Ou e sepal apex Re use Cauda e Acu e Ob use and
muc ona e
Co olla shape and
colo
Campanula e,
whi e
Hypoc a e i o m,
whi e
Funnel o m,
whi e-yellowish
Funnel o m, blue
Colo o he abaxial
mesope al egion
Yellow G eenish Yellow Whi e
Tec o ichomes a
he base and along
he ilamen s
P esen Absen Absen Absen
Pollen size Ve y la ge (x =
110.8 × 109.6 μm)
La ge (x = 79.2 ×
78.8 μm)
La ge (x = 92 ×
91.2 μm)
Ve y la ge (x =
101.1 × 100.8 μm)
Numbe o spines 165 spines 42 spines 55 spines 703 spines
Exine hickness Nexine is hicke
han sexine
Nexine is hicke
han sexine
Sexine is hicke
han nexine
Nexine is hicke
han sexine
sample o pollen ma e ial (Table 1). The pollen
g ains we e ace olyzed ollowing E d man (1960),
wi h modi ica ions as desc ibed by Melhem e al.
(2003). Diame e measu emen s we e aken on 25
pollen g ains o e one week (Salgado-Labou iau
e al., 1965). O he measu emen s (exine hickness
and ape u es) we e aken om en pollen g ains.
The slides ob ained we e inco po a ed in o he
Labo a o y o Plan Mo phology and Palynology
pollen collec ion a he São Paulo S a e Uni e si y,
campus Jabo icabal - UNESP/FCAV, B azil. The
pollen mo phology and e minology we e based
on Pun e al. (2007) and Halb i e e al. (2018).
Cy ogene ic analyses
Fo cy ogene ic analyses, 3 mm lowe buds we e
ixed in Ca noy’s solu ion (3:1 absolu e e hanol/
glacial ace ic acid, / ) o 2 hou s a oom
empe a u e. To p epa e he slides, he lowe
buds we e washed wice wi h dis illed wa e o
5 minu es each, and he an he s we e diges ed in
an enzyma ic solu ion con aining 2% cellulase and
20% pec inase and kep in a humid chambe a
37 °C o 20 minu es. Subsequen ly, he an he s
we e agmen ed on a slide in a d op o 45%
ace ic acid, co e ed wi h a co e slip, and c ushed.
The co e slip was hen emo ed a e eezing in
liquid ni ogen, and he slides we e ai -d ied and
s o ed o h ee days a oom empe a u e. The
slides we e s ained wi h 10 μL o Ch omomycin
A3 (CMA; 0.1 mg/mL) o 1 hou , ollowed by 10
μL o 4’,6-diamidino-2-phenylindole (DAPI; 1 μg/
mL) o hal an hou , was pe o med as desc ibed
by Ba os e Sil a and Gue a (2010), and Ba os
e Sil a e al. (2010). A e washing wi h dis illed
wa e , he slides we e ai -d ied and p epa ed in a
492
A new species o mo ning glo y o B azilian lo a
medium con aining glyce ol/McIl aine bu e pH
7.0 (1:1). The slides we e hen s o ed o 3 days in a
da k oom o s abilize he luo och omes. The bes
cells we e analyzed using a Zeiss pho omic oscope
wi h an Axio Cam MRC5 (Obe kochen, Ge many)
and Axio ision .4.8 so wa e, a he Plan
Cy ogene ics Labo a o y a he Fede al Uni e si y
o Pa aíba, campus A eia - UFPB/CAA, B azil.
Taxonomic ea men
Ipomoea bu iliae Belo, sp. no .
Figs. 2,3
Ipomoea bu iliae is simila o I. pa asi ica in ha ing spiny
p ojec ions along he s em, pe iole, and peduncle, as
well as co da e lea es, and p ominen eins on he
abaxial su ace. Howe e , I. bu iliae can be dis inguished
by i s lea es wi h pubescen indumen um wi h cauda e
apex ( s. se iceous wi h apex acu e), he ou e sepals
o bicula wi h apex e use, and he inne sepals
eni o m wi h he apex obco da e, glab ous ( s. ellip ic
wi h apex ob use and muc ona e – he ou e sepals, and
apex ounded and minu ely muc onula e – he inne
sepals, pube ulen o glab escen ), whi e campanula e
co olla ( s. blue unnel o m), ilamen s wi h ec o
ichomes a he base and along he s uc u e ( s. ec o
ichomes only a he base) (see Table 2).
Type: BRAZIL, Pa aíba, A eia, campus da
Uni e sidade Fede al da Pa aíba, 6°58’10”S,
35°42’50”W, 550 m, 20.07.2024, D. Belo & E. Ba bie
740 (holo PEUFR!; iso CSTR!, EAN!, UFP!).
Climbing plan s; b anches glab ous, s ia ed
wi h spiny p ojec ions; in e nodes 5.5–12.3 cm
long. Lea es o a e, 8.5–15 × 6.1–13 cm, base
co da e, ma gins en i e, apex cauda e, adaxial
su ace pubescen wi h ec o and sessile pel a e
glandula ichomes, abaxial su ace pubescen
o glab escen wi h ec o and sessile pel a e
glandula ichomes, penine ea ena ion, simple
b ochidod omous ype, wi h eigh o en pai s
o seconda y eins; pe ioles 7.2–16 cm long,
glab ous o glab escen wi h ec o and sessile
pel a e glandula ichomes, s ia e. In lo escence
cymose, 3–8- lowe ed; peduncles 9.4–31 cm long,
glab escen , glab ous; b ac eoles linea , 0.3–1
cm long, base ounded, apex acu e, glab ous;
pedicels 1–2 cm long, glab ous, s ia e. Sepals
5, unequal, he 2 ou e ones o bicula , 6.4–6.5 ×
5.8–6.2 mm, base oblique, apex e use, glab ous,
he in e media e one eni o m, c. 11 × c. 10 mm,
he base oblique, he 2 inne ones eni o m,
11.2–12.5 × 11.2–12.6 mm, base co da e, apex
obco da e. Co olla campanula e, 5.8–6.2 cm long,
whi e, glab ous, yellow abaxial mesope al egion.
S amens 5; ilamen s 9.5–17.35 mm long, ec o
ichomes a he base and along he s uc u e;
an he s 5.8–6.9 mm long, oblong, glab ous, pollen
g ains monads, apola , e y la ge size, sphe oidal,
pan opo a e. O a y conical, 1.4–1.5 × 2–2.2 mm,
4-locula ; s yle en i e, 30–34.5 mm long, s igma ic
lobes 2, 1.4–1.8 mm long, 2-capi a e wi h he
su ace e ucose. Capsules globose, 1–2 cm long.
Seeds 0.6–1 cm long, pubescen .
Flowe ing & ui ing: Flowe ing om June o
Augus and ui ing om July o Sep embe .
Habi a : The new species occu s in a humid o es
encla e in Pa aíba s a e, no h o he São F ancisco
Ri e , B azil, a an ele a ion o abou 550 m.
Dis ibu ion: The known dis ibu ion o I. bu iliae
is es ic ed o he ype locali y.
E ymology: The speci ic epi he is named in
hono o D . Ma ia Te esa Bu il, acknowledging
he ou s anding con ibu ions o he s udy o he
sys ema ics and axonomy o Con ol ulaceae,
as well as o he ield o bo any. Beyond being
a no able esea che , D . Bu il is an excep ional
men o , commi ed o os e ing he de elopmen
o new B azilian scien is s.
Addi ional specimens examined (Pa a ypes): BRAZIL,
Pa aíba, A eia, campus da Uni e sidade Fede al
da Pa aíba, 6°58’02”S, 35°42’56”W, 563 m,
21.07.2024, D. Belo & E. Ba bie 741 (PEUFR!);
Ibid.,, 6°58’03”S, 35°42’52”W, 570 m, 24.07.2024,
D. Belo & E. Ba bie 742 (PEUFR!); Ibid., 550 m,
27.08.2024, R. Sil a e al. 10 (EAN!).
Conse a ion s a us: Acco ding o IUCN c i e ia,
despi e occu ing in an an h opic a ea, we
conside he conse a ion s a us o I. bu iliae as
Da a De icien (DD) since i is known only om
493
Belo e al.
Fig. 2
. Ipomoea bu iliae Belo:
a
. Adaxial su ace o he lea blade.
b
. Abaxial su ace o he lea blade.
c
–
d
. S ems and pe ioles wi h spiny p ojec ions.
e
. Co olla shape.
–
g
. In lo escence wi h lowe s and buds.
h
. Sepals.
i
. Habi .
494
A new species o mo ning glo y o B azilian lo a
Fig. 3
. Ipomoea bu iliae Belo:
a
. Fowe ing b anch;
b
. S em wi h spiny p ojec ions;
c
. Adaxial su ace o he lea blade.
d
. Abaxial su ace o he lea
blade.
e
. Abaxial su ace wi h ichomes
. B ac eole; g. Flo al bud.
h
. Flowe .
i
. Sepals [le o igh - ou e (2), middle (1), inne (2)];
j
. Co olla– op
iew;
k
. Co olla spli open showing s amens and s yle;
l
. S amen.
m
. Gynoecium.
n
. O a y.
495
Belo e al.
he ype locali y.
Taxonomic no es: Ipomoea bu iliae esembles
se e al species ound in no heas e n B azil, such
as I. alba L. and I. ma cellia Meisn., which sha e
cha ac e is ics like a whi e co olla, p ominen
eins on he lea blade, and indumen um on he
s em, lea es, pe iole, and peduncle. Howe e ,
ep oduc i e cha ac e s play a signi ican ole
in dis inguishing hese axa, pa icula ly co olla
shape, he colo o he abaxial mesope al egion,
and sepal shape (see Table 2).
Ana omical no es: In he pa ade mic sec ion and
he on al iew, he epide mis o I. bu iliae
p esen s cells wi h s aigh an iclinal walls on
he adaxial su ace (Fig. 4a), and sinuous on he
abaxial su ace (Fig. 4b), wi h d uses es ic ed
o he adaxial su ace (Fig. 4a). The dis ibu ion
o s oma a is amphihypos oma ic, wi h pa acy ic
and anisocy ic s oma a and s oma al g ouping
(Fig. 4c). Tec o and glandula ichomes occu
on bo h epide mal su aces (Fig. 4a & b). In c oss-
sec ion, he epide mis is unise ia e (Fig. 4d), wi h
o al o ec angula cells and ex e nal pe iclinal
walls co e ed by a smoo h cu icle. The lea blade
has an asymme ic do si en al mesophyll (Fig.
4d), p esen ing bise ia e palisade pa enchyma and
spongy pa enchyma 1-3 s a um se ia e. Also in
he mesophyll, idioblas s con aining d uses (Fig.
4e), la ici e ous canals (Fig. 4 ), and p isma ic
c ys als in he palisade pa enchyma we e obse ed
(Fig. 4 ). The main mid ib, in c oss-sec ion,
exhibi s a bicon ex con ou (Fig. 4g), p ominen ly
wide on he abaxial su ace. The epide mis is
unise ia e, wi h ec o ichomes. Unde lying he
epide mis is he lacuna collenchyma (Fig. 4h).
Fu he in e nally, he undamen al pa enchyma
consis s o isodiame ic ci cula cells wi h
la ici e ous canals (Fig. 4h). The ascula sys em is
bicolla e al, consis ing o a single U-shaped cen al
bundle (Fig. 4g & i). The pe iole in c oss-sec ion
p esen s a conca e-con ex con ou (Fig. 4j). The
epide mis is uns a i ied wi h nec a ichomes
and sessile pel a e glandula ichomes. Adjacen
o he epide mis, he co ical egion consis s o
laye s o angula collenchyma wi h d uses (Fig.
4k), ollowed by he undamen al pa enchyma
wi h la ici e ous canals. The ascula sys em is
bicolla e al, composed o i e bundles – h ee
cen al ones o ming an a ch and wo adaxial
accesso ies (Fig. 4j). D uses occu abundan ly in he
in e nal and ex e nal egions o he phloem (Fig.
4l). The lea ana omy o I. bu iliae was compa ed
wi h I. pa asi ica, which is mo phologically
simila . Bo h species exhibi dis inc ege a i e
mo phoana omical cha ac e s ha a e c ucial
o hei delimi a ion. These include di e ences
in he sinuosi y o he an iclinal walls on he
epide mal su ace o he lea blade, s oma al ypes,
he numbe o palisade pa enchyma laye s in he
mesophyll, he ype o collenchyma in he mid ib,
and he shape and numbe o ascula bundles in
he pe iole (Fig. 5, Table 3).
SEM images o he lea es e eal a di e se a ay o
su ace s uc u es. The images o he lea blade and
pe iole highligh he abundan p esence o ec o
ichomes (Fig. 4m & n), which appea as hin,
elonga ed p ojec ions. Sessile pel a e glandula
ichomes a e dis ibu ed ac oss he lea blade,
pe iole, and s em (Fig. 4o–q). Spiny p ojec ions
we e obse ed on he s em (Fig. 4 ), cha ac e ized
by a obus , poin ed s uc u e wi h a b oad base
ha ape s o a sha p ip. SEM images also show
he p esence o cu icula waxes on he adaxial
su ace o he lea blade, appea ing as g anules
(Fig. 4m) and memb anous pla ele s (Fig. 4 ).
Palynological no es: The pollen g ains o I. bu iliae
a e monads, apola and e y la ge (x
= 110.8 × 109.6
μm), wi h a sphe oidal shape. They a e pan opo a e
con aining app oxima ely 90 ci cula po es (x
=
7.1 μm), and lack an annulus. The exine is ec a e,
echina e, and pe o a e ea u ing bulbous spines
(x
= 15 μm), numbe ing a ound 165. These spines
ha e a ounded apex, wi h a wide, polygonal base.
The nexine is hicke han he sexine, and he exine
is hin ( o al exine x
= 7.2 μm; sexine x
= 3.3 μm;
nexine x
= 3.95 μm) (Fig. 6, Table 2).
496
A new species o mo ning glo y o B azilian lo a
Table 3. Ana omical compa ison be ween Ipomoea bu iliae Belo, sp. no . and I. pa asi ica (Kun h) G. Don.
Cha ac e Ipomoea bu iliae Belo, sp. no . Ipomoea pa asi ica (Kun h) G. Don
Epide mal cells (adaxial wall) S aigh Sinuous
Epide mal cells (abaxial wall) Sinuous Sinuous
T ichomes on he lea epide mis Tec o and sessile pel a e
glandula ichomes
Tec o and sessile pel a e glandula
ichomes
Palisade mesophyll Bise ia e Unise ia e
Collenchyma cells o he mid ib Lacuna Angula
S oma a Pa acy ic, anisocy ic s oma a,
and s oma al g ouping.
Pa acy ic
Pe iole shape Conca e-con ex Semici cula
Vascula bundles o pe iole 5, a ch-shaped 13, U-shaped
Cy ogene ic no es: Ipomoea bu iliae has a
ch omosome numbe o 2n = 30. Dis inc GC- ich
he e och oma ic egions a e obse ed on di e en
ch omosomes, o ming 14 e minal CMA+/DAPI-
bands (Fig. 7).
Discussion
Among he Ipomoea species wi h a whi e
co olla ound in he Bo bo ema Pla eau (BP) o
no heas e n B azil, I. bu iliae is dis inguished
by i s campanula e co olla ( s. hypoc a e i o m
in I. alba, unnel o m in I. ma cellia, ubula in
I. espe ilia F.D.San os, G.C.Delgado-Junio &
Bu il). In he ege a i e s age, he new species
may be con used wi h I. pa asi ica, since hey
sha e co da e lea es, spiny p ojec ions on
he s em, and occasionally on he pe iole and
peduncle. Howe e , a de ailed analysis o he
ype specimens and p o ologue o I. pa asi ica
shows ha he species di e mainly in he
lea apex, co olla colo , peduncle size, and he
sepals’ shape, apex, and indumen um. The
compa a i e ana omical analysis e ealed ha
he mo phological delimi a ion o hese species
is s ongly suppo ed mainly by he shape o he
epide mal cell walls o he adaxial su ace, ype
o collenchyma o he mid ib, ypes o s oma a,
shape o he pe iole, dis ibu ion, shape, and
numbe o ascula bundles (see Fig. 5, Table 3).
Scanning elec on mic oscopy e ealed wo ypes
o epicu icula waxes: g anules and memb anous
pla ele s. The e is li le in o ma ion abou he
ypes o waxes ound in Ipomoea. Howe e , in an
ana omical s udy o popula ions o Jacquemon ia
e ol uloides (Mo ic.) Meisn., Belo e al. (2023c)
obse ed he same ypes o waxes, leading us o
in e ha i can be conside ed a cha ac e p esen
in Con ol ulaceae. Waxes a e axonomically
impo an in delimi a ing se e al plan g oups
(Ba hlo e al., 1998) and play an impo an
unc ional ole in educing wa e loss and
p o iding de ense agains pa hogens (Ahmad e
al., 2015). The p esence o hese speci ic waxes’
ypes in I. bu iliae highligh s he signi icance o
ana omical ea u es in delimi ing axonomic
g oups wi hin he Con ol ulaceae amily. This
inding p o ides a aluable ool o sys ema ic
s udies and he iden i ica ion o new species.
Epide mal cells and o he ana omical cha ac e s
may a y acco ding o en i onmen al ac o s (Bone
e al., 1985; Alenca e al., 2022). Howe e , when
cha ac e s a e gene ically de e mined and do no
a y due o abio ic ac o s, hey can be axonomically
503
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