1
Supplemen a y In o ma ion o :
Häll o s, M.H., Robson T.M., Bu g S., Pen ikäinen S., Koi usaa i S. H. M., Luo o M., Nez al J.,
Pech R., Saas amoinen M., Schulman L., Si én J., Susi H.: The mal plas ici y o mul iple ai s
a ies mo e wi hin han be ween popula ions o Plan ago lanceola a a i s no he n ange edge.
Con en s:
Figu es S1-S7
Table S1-S3
Re e ences
2
Figu e S1. June and July empe a u e in Åland, Finland du ing 1960-2020 in ela ion o
expe imen al ea men s. The poin and lines in ligh g ey, g ey and da k g ey show minimum,
median and maximum empe a u es, espec i ely. The lines a e smoo hed condi ional means i ed
using ggplo in R (Wickham, 2016). The dashed ho izon al lines in blue, peach and ed show nigh
(lowe ) and day (uppe ) empe a u es se o he he mal expe imen s o he cold, mean and wa m
ea men s, espec i ely.
3
Figu e S2. Typical ch oma og am o sepa a ed phenolic compounds ob ained om HPLC-DAD
analysis eco ded a 270 nm. HCA – hyd oxycinnamic acid de i a i es; FLV – la onoids.
4
Figu e S3. UV-VIS abso p ion spec a o phenolic compounds de ec ed by HPLC-DAD. A)
la onoid de i a i es, B) hyd oxycinnamic acid de i a i es.
5
Fig S4. Measu ed ai esponse ac oss empe a u e ea men (summa ized ac oss popula ions)
o A) lea size, B) numbe o lea es; C) lowe p esence; D) numbe o lowe s; E) la onoid
con en o peak a ea mAU.s/ d.w. mg; F) HCA con en ; G) blue ligh e lec ion o in lo escence
b ac s; H) p opo ion o lea es wi h pa hogen symp oms; I) numbe o indi iduals wi h o wi hou
PLCaV in ec ion J) numbe o indi iduals wi h o wi hou PlLV in ec ion; K) o al numbe i us
in ec ions pe indi idual (0, 1=ei he PLCaV o PlLV in ec ion; 2=bo h in ec ions). Da a p esen ed
pe popula ion in Fig S4. Es ima ed e ec s in main ex Fig. 2 and Table 1.
6
Fig S5. Measu ed ai esponse ac oss empe a u e ea men (sepa a ely pe popula ion) o
A) lea size, B) numbe o lea es; C) lowe p esence; D) numbe o lowe s; E) la onoid con en
o peak a ea mAU.s/ d.w. mg; F) HCA con en ; G) blue ligh e lec ion o in lo escence b ac s; H)
p opo ion o lea es wi h pa hogen symp oms; I) numbe o indi iduals wi h o wi hou PLCaV
in ec ion J) numbe o indi iduals wi h o wi hou PlLV in ec ion; K) o al numbe i us in ec ions
pe indi idual (0, 1=ei he PLCaV o PlLV in ec ion; 2=bo h in ec ions). Da a p esen ed pe ac oss
popula ions in Fig S3. Es ima ed e ec s pe popula ion in Fig. S7.
7
Figu e S6. Co ela ion plo s o measu ed ai s A) ac oss ea men s, and in he B) cold, C) mean
and D) wa m ea men s. The co ela ion plo s we e c ea ed using he ggpai s unc ion in he
GGally pacakage in R (Schloe ke e al., 2024).
8
Fig S7. Pos e io mean and c edible in e als o he a e age esponse pe ea men
empe a u e and popula ion (di e en colo s). A) Lea size, B) numbe o lea es; C) lowe ing
p obabili y; d) numbe o lowe s, E) la onoid con en ; F) HCA con en ; G) blue ligh e lec ion;
H) p opo ion o lea es wi h pa hogen symp oms; I) p obabili y o PLCaV in ec ion J) p obabili y
o PlLV in ec ion; K) p obabili y o i us in ec ions. Es ima ed e ec s (on he la en , i.e., modelled,
scale) in main ex Table 1. Es ima ed e ec s ac oss popula ions in main ex Fig 2.
9
Table S1. In o ma ion on seed sampling loca ions.
Popula ion
Sampled
indi iduals
Y-coo d
X-coo d
Loca ion
Municipali y
A
50
60.06770
20.071867
Söde by
Lemland
B
50
60.13275
19.989163
Ös e kalma e
Jomala
C
50
60.17423
19.523903
Skepps ik
Ecke ö
D
28
60.22434
19.559542
S o by
Ecke ö
E
17
60.27522
20.240213
Hul a
Sund
F
39
60.25415
20.228615
Mångs ek a
Sund
G
26
60.26042
20.178783
S ömbols ad
Sund
H
20
60.08355
19.906516
G ege sö, Möckelö
Jomala
I
48
60.06605
19.951663
Espholm, Y e näs
Ma iehamn
