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DETERMINATION OF CHANGES IN HEMATOLOGICAL INDICATORS OF
THE BLOOD OF COWS WITH ENDOMETRITIS
Senio doc o al s uden - R.I.Bobomu odo
Scien i ic leade , Doc o o Science - O.U.Kuldashe
Scien i ic esea che - S.Sh.Axmedo
Ve e ina y Resea ch Ins i u e
Anno a siya: Bu maqolada endome i bilan kasallangan sigi la dan olinadigan
qon namunala ini ekshi ish, qon a kibidagi e i o si , leyko si , mono si , ezino il,
ne o illa miqdo ini hamda qon za dobining biokimyo iy ko‘ sa gichla i o‘ ganilib
amaliyo ga egishli xulosala be ilgan.
Abs ac : This a icle examines he examina ion o blood samples aken om
cows wi h endome i is, he le els o e y h ocy es, leukocy es, monocy es, eosinophils,
neu ophils in he blood, and biochemical pa ame e s o blood se um, and p o ides
p ac ical conclusions.
Kali so‘zla i: Leyko o mula, e i o si , leyko si , mono si , ezino il, ne o illa ,
kislo a, ishqo , ka o in, albumin, gammaglobulin, mono si openiya, ezis en lik,
aga si oz.
Keywo ds: Whi e blood cell coun , e y h ocy e, leukocy e, monocy e,
eosinophil, neu ophil, acid, alkali, ca o ene, albumin, gammaglobulin,
monocy openia, esis ance, phagocy osis.
In oduc ion. Today, in e ili y and gynecological diseases, including
endome i is, a e widesp ead among p oduc i e cows wo ldwide. One o he p oblems
hinde ing he de elopmen o li es ock a ming in ou epublic is in e ili y and
gynecological diseases in a m animals, which cause signi ican economic damage o
li es ock a ms. “E e y yea , 20-30% o cows and 30-40% o e ile-age cows emain
in e ile in li es ock a ms o ou epublic. Symp oma ic in e ili y o cows is 18.3%,
alimen a y in e ili y is 15.5%. Based on his, one o he u gen asks is o s udy he
p ocess o he sp ead o in e ili y and pos pa um gynecological diseases in he
li es ock sec o , o de elop highly e ec i e ea men and p e en i e measu es.
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In mos coun ies o he wo ld, in e ili y and pos pa um gynecological diseases
among p oduc i e cows a e common, due o he lack o imp o ed eed a ions,
me abolic diso de s in he body, a ious s ess ac o s and unsani a y condi ions,
which lead o a dec ease in he body's na u al esis ance, signi ican changes in blood
composi ion, which leads o an inc ease in he pa hological p ope ies o
mic oo ganisms and he appea ance o seconda y diseases in he body.
In ou epublic, he implemen a ion o la ge-scale measu es aimed a educing,
ea ing and p e en ing in e ili y and pos pa um gynecological diseases in li es ock,
especially in p oduc i e cows, emains one o he impo an issues in he e e ina y
ield. In he de elopmen and imp o emen o ca le b eeding, which is he main
b anch o animal husband y, i is impo an o inc ease he numbe o cows in a ms
and p i a e a ms, inc ease hei p oduc i i y, p ope ly ca e o hem and p o ec hem
om a ious in ec ious, in asi e and non-in ec ious diseases in o de o ob ain heal hy
cal es.
Ma e ials and me hods: As he ma e ials o ou esea ch, 5-10 ml o blood
was aken om he jugula ein o expe imen al and con olled cows kep a he
"Siyob Sha ka O zu" a m in he Tayloq dis ic o he Sama kand egion, in o
hepa inized es ubes, and he mo phological and se um biochemical pa ame e s o he
blood we e examined du ing ea men .
Du ing he es s, i was ound ha he numbe o e y h ocy es in he blood o
cows in he expe imen al g oup was highe han ha o cows in he con ol g oup. The
expe imen s e ealed a dec ease in hemoglobin, a comple e absence o leukocy es and
monocy es, and a dec ease in eosinophils and neu ophils. The hemoglobin con en in
a sick animal was 10.53 g%, and in a heal hy animal i was 9.70 g%. I was obse ed
ha he numbe o e y h ocy es in he blood o sick animals was 6.82 million, and in a
heal hy animal his igu e was 5.53 million. The numbe o leukocy es in he blood o
sick animals was 8.60 housand, and in he blood o a heal hy animal was 5.38
housand. The main eason o he inc ease in he numbe o eosinophils and
lymphocy es in he leuko o mula is ha eosinophils inc eased in o de o neu alize
p o ein oxins o med as a esul o in lamma ion in he u e us; lymphocy es inc eased
in o de o p oduce an ibodies o mic oo ganisms in he body. The inc ease in
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monocy es in he blood, in u n, can be explained by hei pa icipa ion in he
eplacemen and es o a ion o dead issues. The numbe o od-shaped and segmen ed
neu ophils in he blood o sick animals is 2.70-18.20, while in he blood o heal hy
animals i eaches 7.0-25.83, ha is, hey a e less in he blood o sick animals (Table
1).
The low numbe o od-shaped and segmen ed neu ophils in he blood o sick
animals indica es ha an in lamma o y p ocess is aking place in he body and ha
hei dec ease is due o he ac ha mos o hem a e des oyed du ing he p ocess o
phagocy osis o mic oo ganisms in he in lamma o y ocus.
Hema ological pa ame e s o bo ine blood
1- able
Indica o s
he ds o ca le
Ca le wi h
endome i is
Clinically heal hy ca le
Hemoglobin, g %
10,53±0,7
9,80 + 0,40
9,70 ± 0,30
E y h ocy es million.
8,60 + 0,92
5,10 + 0,52
5,38 + 0,54
Leukocy es spindle
6,82 + 1,49
5,91 + 0,68
5,53 + 0,56
Lymphocy es %
70,10 + 9,20
73,20 + 6,04
58,08 + 8,38
Monocy es %
-
-
Eosinophils %
9,00 + 1,40
5,00 + 0,12
7,35 + 2,61
Young neu ophils
-
-
Rod-shaped
2,70 + 1,70
14,60 + 4,10
7,00 + 2,01
Segmen co e
18,20 + 7,80
7,20 + 3,40
25,83 + 6,60
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No e: The op line is be o e ea men ; he bo om line is a e he animal has
eco e ed clinically..
Biochemical pa ame e s o blood and se um o cows wi h endome i is indica e
o some ex en he cou se o he disease, he me abolism o p o eins and ca bohyd a es
in he animal's body, he a io o diges ible ca o ene and acid-base en i onmen . In he
ini ial pe iod o he disease, a dec ease in o al p o ein and albumin, an inc ease in be a
and gamma globulins indica e he es o a ion o immunological p ocesses in he body.
Du ing his pe iod, an inc ease in blood suga le els and high bioene ge ic p ocesses
indica e good p o ec i e ac i i y o he body. A dec ease in he amoun o ca o ene
con ibu es o he accumula ion o e men s in he body's me abolism, and an inc ease
in he acidosis p ocess, in u n, inc eases he in lamma o y p ocess in he body (Table
2). In he leuko o mula, a p opo ional inc ease in eosinophil leukocy es and
lymphocy es (lymphocy osis) and a dec ease in monocy es (monocy openia) a e
explained.
Some biochemical pa ame e s o cow blood se um
2- able
Indica o s
numbe o ca le
Cows wi h endome i is
Clinically heal hy ca le
Ino ganic phospho us
6,55 + 0,33
5,81 + 0,26
5,48 + 0,36
Calcium, mg %
11,05 + 0,49
10,80 + 0,39
7,30 + 0,22
Ca o ene, mg %
0,32 + 0,08
0,28 + 0,05
0,64 + 0,07
Alkaline ese e SO2
39,29 + 2,07
42,44 + 2,16
42,30 + 4,08
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To al p o ein
7,09 + 1,39
7,71 + 0,31
7,29 + 0,21
Albumins %
37,11 + 2,28
31,32 + 7, 39
40,02 + 4,01
Alpha – globulins %
10,20 + 1,05
10,77 + 3,33
10,82 + 2,11
Be a – globulins %
13,33 + 2,17
15,64 + 1,45
12,00 + 2,35
Gamma – globulins %
39,36 + 2,22
42,27 + 3,02
37,16 + 4,19
Suga , mg %
91,66 + 8,87
48,24 + 8,89
60,70 + 5,87
No e: Top ow - be o e ea men ; bo om ow - a e he animal has eco e ed
clinically.
When he ea men p ocess is e ec i e, ha is, du ing he pe iod o clinical
eco e y, p o ein me abolism e u ns o no mal, and only an inc ease in lymphocy es
(lymphocy osis) is obse ed in he leukog am, which, in u n, inc eases he body's
esis ance and indica es a posi i e cou se o he in lamma o y p ocess. Analysis o he
me abolic p ocesses in he body o cows wi h endome i is shows ha he cou se o he
disease depends on he cou se o me abolism in he body. The e o e, i is necessa y o
o ganize he ea men and p e en ion o his pa hological p ocess based on he abo e
da a.
Resul s and hei analysis: Du ing ou s udies, he ollowing esul s we e
ob ained om mo phological examina ions o he blood o cows wi h endome i is and
heal hy cows; hemoglobin in he blood o sick animals was 10.53 g%, while in heal hy
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animals i was 9.70 g%, e y h ocy es in sick animals we e 8.60 million, in heal hy
animals 5.38 million, and leukocy es in heal hy cows we e 6.82 housand, and in
heal hy animals 5.53 housand. The amoun o hese indica o s in he blood o sick
animals was somewha highe han in he blood o heal hy animals, bu an inc ease in
lymphocy es and eosinophils was obse ed in he leuko o mula, while a dec ease in
od-nuclea ed and segmen ed-nuclea ed neu ophils was obse ed. I was also ound
ha he biochemical pa ame e s o he blood se um o a clinically heal hy animal we e
a e age and below a e age, and he acidi y le el was below he physiological no m.
Conclusion: Du ing ou esea ch, many changes we e ound in he blood
composi ion o cows wi h endome i is. In addi ion, i was ound ha cu en ly, in
many li es ock a ms in ou coun y, heal hy cows ha e some biochemical indica o s
and blood acidi y le els ha a e no a he no mal le el, which a e ac o s ha cause
cows o become ill wi h endome i is in he win e and sp ing seasons. Based on his,
when choosing a me hod o ea ing endome i is, i is necessa y o aim o use d ugs
ha inc ease he body's esis ance.
Lis o used li e a u e:
1. Tu chenko A.N. E iologiya i lechenie posle odo ogo endome i a u ko o // J.
Ve e ina iya. 2001. № 7. -S. 33-37.
2. Bobomu odo , R. I., and Kuldashe , O. U. (2025). diagnosis and ea men
me hods o mas i is (li e a u e e iew). educa o insigh s: jou nal o eaching heo y
and p ac ice, 1(4), 215-221.
3. A aku bano SH. B. Eshbo‘ iye B. M. “Hay onla ko‘payish bio exnikasi”
Sam QXI. Sama qand 2012 y.
4. Bobomu odo , R. I., and Kuldashe , O. U. "Sigi la klinik a subklinik mas i la
diagnos ikasida ezko mas i is la idan oydalanish." In e na ional con e ence on
mode n science and scien i ic s udies. 2025.
5. Bagmano M. A. Akushe sko-ginekologicheskaya pa ologiya ko o
(diagnos ika, kompleksnaya e apiya i p o ilak ika).-O‘lyano sk, 2005. 207s.
6. Bobomu odo , R. I., and Kuldashe , O. U. (2025). mas i la ni ashxis a
da olash usulla i (adabiyo la sha hi). o'qi u chi ushunchala i: o'qi ish naza iyasi a
amaliyo i ju nali , 1 (4), 215-221.
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