231
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
QUANTITATIVE ANALYSIS OF ASCORBIC ACID IN THE AERIAL PARTS OF
BIDENS FRONDOSA L.
Abdullae a R.A.
Fa mano a N.T.
Abdullae a X.Kh.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333358
Rele ance. Medicinal plan s con ain a complex o chemical compounds ha ha e a di e se and
mul i ace ed e ec on he human body. To p ope ly assess hei he apeu ic e ec , i is necessa y,
i s o all, o analyse he p ope ies o he main biologically ac i e subs ances in hei composi ion.
Plan s wi h an i-alle gic and an i-in lamma o y e ec s a e always in demand in adi ional medicine,
and ep esen a i es o such plan s include, in pa icula , species o he As e aceae amily, including
Bidens ondosa L. Acco ding o he li e a u e, he ae ial pa o he Bidens ondosa L. is ich in
a ious biologically ac i e subs ances. In pa icula , i con ains la onoids and hei glycosides,
polysaccha ides, annins, essen ial oils, bi e subs ances, asco bic acid, okanin, α- ocophe ol and
lu eolin. The complex composi ion o hese subs ances de e mines he pha macological ac i i y o
he plan . In pa icula , i amins such as asco bic acid and α- ocophe ol exhibi he an ioxidan
ac i i y o he plan , and he combina ion o hese biologically ac i e compounds con i ms he
medicinal p ope ies o he Bidens ondosa L. and jus i ies i s use in a ious pa hological condi ions.
Pu pose o he s udy: The pu pose o he s udy is o conduc a quan i a i e analysis o asco bic
acid and he main biologically ac i e subs ances wi h he apeu ic e ec s in Bidens ondosa L.
Ma e ials and me hods: The abo e-g ound pa s o he plan Bidens ondosa L., collec ed in
he Tashken egion (Kib ai dis ic , Augus –Sep embe 2023–2024) and d ied unde na u al
condi ions by ai d ying a a empe a u e no exceeding 35–40 °C. Asco bic acid, which belongs o
wa e -soluble i amins, was de e mined in he sample using high-pe o mance liquid ch oma og aphy
(HPLC, Agilen -1200, equipped wi h an au o-dispense ). A 5–10 g sample o plan ma e ial, weighed
on analy ical scales, was placed in a 300 ml la -bo omed lask. Ex ac ion was ca ied ou wice
using a wa e -alcohol ex ac an . The esul ing il a es we e combined, placed in a 100 ml olume ic
lask and b ough o he ma k wi h 40% e hanol (5–10% o he olume). The esul ing solu ion was
cen i uged a 7000 pm o 10 minu es. The supe na an pa o he solu ion was used o analysis.
Figu e 1. Ch oma og am o he wo king s anda d solu ion o asco bic acid
Wo king solu ions o wa e -soluble i amins wi h a concen a ion o 1 mg/ml we e p epa ed.
Resul s: Ini ially, wo king s anda d solu ions we e in oduced in o he ch oma og aph,
ollowed by p epa ed wo king solu ions (Figs. 1, 2).
232
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Figu e 2. Ch oma og am o asco bic acid con en in he abo e-g ound pa o Bidens
ondosa L.
Conclusions: Acco ding o he esul s ob ained, he amoun o asco bic acid in he Bidens
ondosa L. aw ma e ial was 14.83 mg/g.
