235
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DEVELOPMENT OF A METHODOLOGY FOR THE ANALYSIS OF SOME
PSYCHOTROPIC SUBSTANCES BY THE TLC METHOD
Abdullaye a M.U.
Olimo N.K.
Sidame o a Z.E.
She ma o a M.I.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333411
Rele ance. The s udy o medicinal p oduc s con aining psycho opic subs ances should be
ca ied ou using a simple, accessible, and apid me hod - hin-laye ch oma og aphy. The ad an ages
o he me hod - apid sepa a ion, high esolu ion, sensi i i y, and ease o so ben p epa a ion - a e he
main easons o i s widesp ead use in he expe analysis o nume ous pha maceu ical p oduc s and
complex medicinal mix u es. The TLC me hod o e s signi ican ad an ages o e chemical me hods,
pa icula ly when analyzing mul icomponen d ug mix u es. I enables he sepa a ion and
iden i ica ion o indi idual cons i uen s, as well as subs ances wi h simila chemical s uc u es and
p ope ies.
The aim o ou esea ch is o de elop a me hodology o in es iga ing ce ain psycho opic
subs ances equen ly encoun e ed in o ensic p ac ice using he TLC me hod. The selec ion o a
sol en sys em is ca ied ou aking in o accoun he pola i y o he s udied compounds, as well as he
ac i i y o he so ben . When sepa a ing subs ances wi h simila abso p ion p ope ies, a mix u e o
sol en s loca ed close o each o he in he eluo opic se ies should be used. The con en o he mo e
pola sol en in his mix u e ypically anges om 1 o 10%. The inal choice o sol en sys em is
achie ed h ough expe imen al analysis.
Ma e ials and Me hods. The s udy subjec s we e s anda d solu ions o psycho opic
subs ances o he benzodiazepine se ies. The analysis was pe o med using a labo a o y hin-laye
ch oma og aphy equipmen se consis ing o an ul a iole ligh sou ce (illumina o ), an iodine
chambe , UV-254 silu ol able s, D agendo -Mounie eagen , an MS-10 mic osy inge, a c ucible,
a sp aye , and o ganic sol en s: benzole, e hanol, die hylamine, chlo o o m, and e hyl ace a e.
Psycho opic able s a e g ound o a powde and used o p epa e 0.1% and 0.2% alcohol
solu ions.
Alcohol solu ions a e il e ed h ough il e pape and analyzed using hin-laye
ch oma og aphy.
Resul s. The esul s o a s udy o he ch oma og aphic mobili y o psycho opic benzodiazepine
de i a i es in hin laye s o a benzole-e hanol-die hylamine (9:1:1) so ben sys em a e p esen ed in
Table 1.
Table 1.
N
Name
R Glow
Unde UV
ays
De ec ion in
iodine apo
D agendo
eagen
1
2
3
4
5
Alp ozalam
B omazepam
Diazepam
Clonazepam
Lo azepam
0,45
0,57
0,51
0
0,44
ligh lilac
- " -
da k iole
-
lilac
b igh yellow
- " -
whi e
-
yellowе
o ange
o ange
yellow
-
o ange
236
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Analysis o he able da a indica es ha he elu ion capaci y o his sol en sys em a ies o he
psycho opic subs ances s udied. Fo example, he sys em is ideal o sepa a ing alp azolam,
b omazepam, diazepam, and lo azepam, bu is ine ec i e o clonazepam.
Conclusions. A sys em consis ing o benzole - e hanol - die hylamine = 9:1:1 is ideal o he
sepa a ion o psycho opic subs ances - benzodiazepine de i a i es alp azolam, b omazepam,
diazepam, lo azepam. In his case, hei R alues, he colo o he spo s when illumina ed by UV
ays, de eloped in iodine apo and wi h he D agendo eagen we e de e mined.
