243
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
LOPHANTHUS ANISATUS (BENTH) ANALYSIS OF QUERCETIN CONTENT IN
THE AERIAL PARTS
Ib agimo a D.M.
Fa mano a N.T.
Ib agimo a S.B.
Jumaqulo a D.J.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333537
Rele ance: Acco ding o he da a epo ed in he li e a u e, he aw ma e ial o lophan hus
anisa us (Ben h.) exhibi s immunomodula o y ac i i y and con ibu es o as e eco e y om
complica ions o pneumonia, b onchi is, and b onchial as hma. The ae ial pa s o lophan hus anisa us
(Ben h.) con ain la onoids, among which que ce in occupies a p ominen posi ion. Que ce in is
classi ied as a i amin P compound and is ecognized as a po en an ioxidan .
Resea ch Aim: De e mina ion o que ce in in lophan hus anisa us (Ben h.) using High-
Pe o mance Liquid Ch oma og aphy (HPLC).
Ma e ials and Me hods: The objec o he s udy was he ae ial pa s o lophan hus anisa us
(Ben h.), cul i a ed in 2024. The plan aw ma e ial was collec ed in acco dance wi h gene ally
accep ed pha macognos ic equi emen s and d ied in a shaded a ea. To iden i y he la onoids in he
plan ma e ial, High-Pe o mance Liquid Ch oma og aphy (HPLC) was employed. The analysis was
ca ied ou using an ‘Agilen 1260 Se ies’ HPLC sys em (Agilen Technologies, USA). The analysis
was pe o med on an Agilen 1200 C18 column (4.6 mm inne diame e , 150 mm leng h, 5 μm pa icle
size). The mobile phase consis ed o 0.3% o hophospho ic acid (A) and me hanol (B). De ec ion was
ca ied ou a 370 nm, co esponding o he cha ac e is ic abso p ion maximum o que ce in. The low
a e o he mobile phase was 1 mL/min, wi h an injec ion olume o 10 μL, and he column
empe a u e main ained a 40 °C. The o al un ime o he analysis was 20 minu es.
Resul s:
1- igu e. Ch oma og am o que ce in (ISN)
2- igu e. Ch oma og am co esponding o que ce in in Lophan hus anisa us (Ben h).
As can be seen om he ch oma og am p esen ed abo e (Figu es 1 and 2), he e en ion ime
o he que ce in s anda d sample, de e mined using a UV de ec o a a wa eleng h o 371 nm, was
11.623 minu es.
244
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions: The ae ial pa s o lophan hus anisa us (Ben h.) exhibi ed a cha ac e is ic
ch oma og aphic peak o que ce in a a wa eleng h o 371 nm wi h a e en ion ime o 11.472
minu es, which co esponded o ha o he wo king s anda d solu ion. This inding con i ms he
p esence o que ce in in he plan aw ma e ial. The quan i a i e de e mina ion e ealed ha he
que ce in con en amoun ed o 19.8 mg/g.
