257
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF THE MONOSACCHARIDE COMPOSITION BY PAPER
CHROMATOGRAPHY METHOD FROM ACORUS CALAMUS L., HELICHRYSI
MARACANDICUM POP EX KIRP. AND AERVA LANATA L.
Mahmudo a.L.A.
Khusaino a.R.A.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333711
Rele ance: Plan polysaccha ides a e mul i unc ional biopolyme s wi h a wide ange o
pha macological ac i i ies. Wa e -soluble polysaccha ides (WSP), pec in subs ances (PS), and
hemicelluloses (HC) dese e special a en ion because o hei an ioxidan , immunomodula o y, an i-
in lamma o y, and wound-healing p ope ies. The plan s Aco us calamus L., Helich ysi
ma acandicum Pop ex Ki p. and Ae a lana a L. a e widely used in olk and adi ional medicine, bu
hei combined composi ion has no been s udied o he i s ime.
The aim o his s udy was o de e mine he monosaccha ide composi ion o he ex ac s
ob ained om he ollowing plan s: Aco us calamus L., Helich ysi ma acandicum Pop ex Ki p., and
Ae a lana a L. using pape ch oma og aphy.
Ma e ials and me hods: Di e en polysaccha ide g oups we e sepa a ed using he abo e-
desc ibed me hod. Ch oma og aphic analysis e ealed ha he alcohol-soluble suga s consis ed o
uc ose, glucose, and suc ose. Wa e -soluble polysaccha ides we e ex ac ed using wa e ex ac ion.
Fi s , wa e -soluble polysaccha ides (WSP) we e isola ed, ollowed by he sequen ial isola ion o
pec in subs ances (PS) and hemicelluloses (HMC). Be o e analysis, he aw ma e ials we e cleaned
o o eign subs ances. One hund ed g ams o c ushed aw ma e ials (Aco us calamus L. ( oo ),
Helich ysi ma acandicum Pop ex Ki p. A. lana a L.( lowe s) and Ae a lana a L. (abo e-g ound
pa s), and h ee es mix u es we e ea ed wice wi h a ho mix u e o me hanol and chlo o o m (1:1)
o emo e dyes and non-ca bohyd a e componen s. The aw ma e ials we e hen sepa a ed by
il a ion and d ied a e wa d. The isola ed subs ances we e analyzed using pape ch oma og aphy
(PC) Fil ac-FN 13, 18 (Ge many). The ollowing sol en s we e used in he ollowing a io: n-
bu anol-py idine-wa e (6:4:3). The ollowing eagen s we e used o opening: 1) acid ph hala e
aniline (5 min, a 100°C), and 2) 5% u ea solu ion.
Resul s: The d ied aw ma e ial was ex ac ed wice wi h ho e hyl alcohol (1:6) a 82 °C o 1
h. The alcohol ex ac s we e combined, e apo a ed, and analyzed ch oma og aphically in sys em 1,
esul ing in he de ec ion o aces o glucose, suc ose, and uc ose. A e comple e acid hyd olysis
o WSP, PS, and HMC, 100 mg o he isola ed polysaccha ides we e hyd olyzed wi h 3 ml o 1n
H2SO4 solu ion a 100°C: WSP o 8 h and PS and HMC o 24 h. A e he speci ied ime, he
hyd olysa e was placed in a beake and neu alized wi h ba ium ca bona e. The esul ing p ecipi a e
was il e ed, and he il a e was deionized wi h KU-2 ca ioni e, e apo a ed o a small olume (0.5
ml), and ch oma og aphed on FN-18 pape in an n-bu anol-py idine-wa e (6:4:3) sys em wi h
speci ic monosaccha ides (con ol samples). A e d ying, he ch oma og ams we e ea ed wi h acid
aniline ph hala e and hea ed in an o en a 110°C o 1-2 minu es. Galac ose, a abinose, and glucose
we e ound in he monosaccha ide composi ion o he polysaccha ides.
Conclusion: The monosaccha ide composi ion o polysaccha ides in he ex ac s ob ained om
he collec ion o plan s Aco us calamus L., Helich ysi ma acandicum Pop ex Ki p., and Ae a lana a
L. was s udied using pape ch oma og aphy. Gi en ha hei composi ion is e y simila o ha o
258
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
he plan s s udied sepa a ely, as well as he p ese a ion o high quan i a i e p oduc i i y, hese plan
collec ions can be used as e ec i e biological p oduc s in he u u e.
