336
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
SYNTHESIS AND CHARACTERIZATION OF HYDROXYAPATITE
NANOPARTICLES FOR BIOMEDICAL APPLICATIONS
J.T. Abdusalamo 1
M.K. Suyundiko 1
Sh.I. Azizo 1
M.A. Sha ipo 2
A.S. Tu aye 1
1Ins i u e o Bioo ganic Chemis y, Tashken ci y, Republic o Uzbekis an
2Facul y o Mechanical Enginee ing, Yonsei Uni e si y. Seul, Republic o Ko ea
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17337277
Pu pose o he s udy:
In his s udy, he syn hesis o hyd oxyapa i e (HA) is epo ed. Cu en ly, in addi ion o he
adi ional (o al and injec ion) me hods o d ug deli e y o he body, deli e y using some chemical
means (micelles, liposomes, e c.) is mo e p e e ed by esea che s. When using hese ools, he
ad an ages o such subs ances a e ha hey can educe he cy o oxici y o chemo he apeu ic agen s,
educe he amoun o adminis e ed d ugs, a ge deli e y, and o he ea u es. In ou s udies, he
nanopolyme s used o d ug deli e y encoun e ed some ba ie s in c ossing he co nea o he skin.
When HA nanopa icles we e used hey p o ided mechanical s eng h due o hei c ys alline
s uc u e. A hyd o he mal syn hesis me hod was used o syn hesize HA nanopa icles.
Ma e ials and me hods:
Ca(NO3)2*2H2O and (NH4)2HPO4 (Sigma Ald ich) sal s and hyd o he mal syn hesis me hod
we e used o syn hesize HA nanopa icles. A mix u e o Ca(NO3)2*2H2O and (NH4)2HPO4 sal s (in
a a io o 0.4:0.1 moles, espec i ely) was dissol ed in 20 mL o dis illed wa e and s i ed using a
magne ic s i e o 30 minu es a oom empe a u e. The esul ing solu ion had a pH o 3 (0.1 M
HNO3 was used o adjus he pH). A e adding he acid, he clea solu ion u ned in o a colloidal
suspension. The o al olume o he suspension was educed o 25 mL.
The eac ion was allowed o p oceed o 72 hou s a 60 °C. A e comple ion o he eac ion,
he esul ing p oduc was collec ed by cen i uga ion (10000 pm, 10 minu es, 3 imes). The ob ained
p ecipi a e was washed wi h e hyl alcohol and d ied in an o en a oom empe a u e.
Resul s:
In his s udy, HA pa icles we e syn hesized using he hyd o he mal me hod. Ou eam modi ied
his me hod by changing he empe a u e and eagen concen a ion. These modi ica ions ha e been
used o p oduce HA nanopa icles anging in size om nanome e s o mic ome e s. Unde op imized
condi ions, we we e able o ob ain HA nanopa icles wi h a hyd odynamic size dis ibu ion o
app oxima ely 270 nm (Figu e 1). The syn hesized HA nanopa icles we e i s sc eened using a ze a-
size o measu e hei hyd odynamic size. The size and mo phology o HA nanopa icles we e
analyzed using a SEM ins umen (Jeol JSM-IT200LA) a he Uzbekis an-Japan You h Inno a ion
Cen e .
337
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions:
In conclusion, i can be said ha when he ca ie d ug was bound o HA nanopa icles, i was
obse ed ha he mechanical s eng h was imp o ed compa ed o he p e ious s a e. O cou se, his
s eng h was ound o depend on he pa icle size o he HA nanopa icles, and u he esea ch aimed
o de elop nanopa icles o highe quali y using o he syn hesis me hods.
0500 1000
0
1
2
3
4
5
6
7
F equency
Size (nm)
A e age size = 270 nm
SD = 130 nm
