354
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
COMPARATIVE ANALYSIS OF EXTRACTION METHODS FOR
STANDARDIZING GREEN WORMWOOD HERB
Kai o a B.A.
Mu a alie a A.D.
Rud N.K.
I.K. Akhunbae Ky gyz S a e Medical Academy, Bishkek ci y, Republic o Ky gyzs an
Sain Pe e sbu g S a e Uni e si y o Chemical Technology, Sain Pe e sbu g ci y, Russian
Fede a ion
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17337525
Rele ance: G een wo mwood he b (A emisia i idis Willd.) is o conside able in e es in
pha maceu ical p ac ice due o i s p onounced biological p ope ies: an i-in lamma o y, an isep ic,
and chole e ic. One o he key s ages o s anda diza ion is he selec ion o he op imal ex ac ion
me hod ha ensu es a high yield o biologically ac i e subs ances while main aining hei s abili y.
In his ega d, he s udy and compa a i e e alua ion o a ious ex ac ion me hods is a ele an ask.
This s udy p o ides a compa a i e assessmen o he e ec i eness o a ious me hods o ex ac ing
la onoids om g een wo mwood he b collec ed in he highlands o Ky gyzs an. The s udy included
ul asonic ex ac ion and Soksle ex ac ion. Quan i a i e de e mina ion o he o al la onoid con en
in g een wo mwood he b was pe o med on a Shimadzu spec opho ome e a a wa eleng h o 410
nm. Using que ce in as a s anda d, in acco dance wi h he me hodology ecommended by GF RF XV.
Resea ch objec i e. To de e mine he mos e ec i e me hod o ex ac ing g een wo mwood
he b o ob ain ex ac s wi h he maximum con en o biologically ac i e subs ances sui able o
s anda diza ion and u he pha maceu ical use.
Ma e ials and me hods: The objec o he s udy was g een wo mwood he b collec ed in he
Na yn egion o he Ky gyz Republic a an al i ude o 2700-3000 m abo e sea le el in Augus 2024.
Ul asonic ex ac ion: ex ac ion was ca ied ou in an ul asonic ba h a a empe a u e o 40°C
o 30 minu es. Wa e -alcohol solu ions o e hanol wi h concen a ions o 40%, 70%, and 96% we e
used as ex ac an s. Socsle appa a us: ex ac ion was ca ied ou o 6 hou s using a mix u e o
e hanol:chlo o o m:HCl (1:1:0.1). Quan i a i e de e mina ion o he o al la onoid con en was
ca ied ou spec opho ome ically using he aluminum chlo ide me hod. The analysis was pe o med
on a Shimadzu UV-1800 spec opho ome e (Japan) a a wa eleng h o 410 nm. The calib a ion cu e
was cons uc ed using que ce in as a s anda d sample.
Resul s. Compa a i e analysis showed ha eplacing a simple e hanol ex ac an wi h a mix u e
o e hanol: chlo o o m:HCl in he Soxhle me hod signi ican ly inc eases he ex ac ion o la onoids.
Chlo o o m p omo es he dissolu ion o lipophilic componen s, and acid p omo es he hyd olysis o
glycosides, eleasing aglycones, he eby inc easing he o al la onoid con en in he ex ac . This
e ec explains he highe alues ob ained by spec opho ome ic de e mina ion compa ed o
ul asonic ex ac ion.
Conclusion. Based on he s udy, i was ound ha he bes esul s o he la onoid con en in
g een wo mwood he b a e achie ed by Soxhle ex ac ion using a mix u e o e hanol:chlo o o m:HCl
(1:1:0.1). This me hod allows o he mos e icien ex ac ion o bo h pola and nonpola la onoid
compounds and can be ecommended o s anda dizing he echnology o ex ac ing biologically
ac i e subs ances om g een wo mwood he b.
