Table S1. Mi ogenomes used o mi ogenome phylogeny in his s udy.
Species
Genbank numbe
O de
Family
Sub amily
Scle ophasma pa esisensis
DQ241798
Man ophasma odea
-
-
G yllobla a sculleni
DQ241796
G yllobla odea
-
-
Pa asipyloidea ca ina a
PP432625
Phasma odea
Lonchodidae
Nec osciinae
Pachyscia longicauda
PP654375
Phasma odea
Lonchodidae
Nec osciinae
S heneboea epudiosa
OQ682531
Phasma odea
Lonchodidae
Lonchodinae
D yococelus aus alis
AP018522
Phasma odea
Lanceoce ca a
-
En o ia okinawaensis
AB477459
Phasma odea
Phasma idae
Cli umninae
Ph ao es illepidus
AB477460
Phasma odea
Lonchodidae
Lonchodinae
Pulch iphyllium gigan eum
AB477461
Phasma odea
Phylliidae
Phylliinae
O es es mouho ii AB477462 Phasma odea He e op e ygidae Da aminae
Ramulus i egula i e den a us
AB477463
Phasma odea
Phasma idae
Cli umninae
Ph ao es sp. I iomo e Island
AB477464
Phasma odea
Lonchodidae
Lonchodinae
Ph ao es sp. Miyako Island
AB477465
Phasma odea
Lonchodidae
Lonchodinae
Micadina phluc ainoides
AB477466
Phasma odea
Lonchodidae
Nec osciinae
Lanceoce ca a sp.
AB477467
Phasma odea
Lanceoce ca a
-
He e op e yx dila a a
AB477468
Phasma odea
He e op e ygidae
He e op e yginae
Neohi asea japonica
AB477469
Phasma odea
Lonchodidae
Nec osciinae
Sipyloidea sipylus
AB477470
Phasma odea
Lonchodidae
Nec osciinae
Megac ania alpheus adan
AB477471
Phasma odea
Lanceoce ca a
-
Ex a osoma ia a um
AB642680
Phasma odea
Lanceoce ca a
-
Timema cali o nicum
DQ241799
Phasma odea
Timema idae
-
Ramulus hainanense
FJ156750
Phasma odea
Phasma idae
Cli umninae
Bacillus a icus
GU001955
Phasma odea
Bacillidae
Bacillinae
Bacillus ossius
GU001956
Phasma odea
Bacillidae
Bacillinae
C yp ophyllium ibe ense
KX091862
Phasma odea
Phylliidae
Phylliinae
Cal isia medogensis
KY124330
Phasma odea
Lonchodidae
Nec osciinae
Megalophasma g anula um
KY124331
Phasma odea
Lonchodidae
Lonchodinae
C yp ophyllium ibe ense
KY124332
Phasma odea
Phylliidae
Phylliinae
Micadina b achyp e a
MT025192
Phasma odea
Lonchodidae
Nec osciinae
Pha naciini sp.
MT025193
Phasma odea
Phasma idae
Pha naciinae
C yp ophyllium wes woodii
MW229063
Phasma odea
Phylliidae
Phylliinae
O es es guangxiensis
MW450873
Phasma odea
He e op e ygidae
Da aminae
Pe uphasma schul ei
MW450874
Phasma odea
Pseudophasma idae
Pseudophasma inae
Ph yganis ia guangxiensis
MW450875
Phasma odea
Phasma idae
Pha naciinae
Eu ycan ha calca a a
MW915467
Phasma odea
Lonchodidae
Lonchodinae
O home ia sma agdinum
MZ312645
Phasma odea
Aschiphasma idae
Aschiphasma inae
Nanhuaphasma hamice cum
MZ312646
Phasma odea
Aschiphasma idae
Aschiphasma inae
Neohi asea s ephanus
OL405132
Phasma odea
Lonchodidae
Nec osciinae
Sosibia gibba
OM257176
Phasma odea
Lonchodidae
Nec osciinae
Sosibia o a a
OM257177
Phasma odea
Lonchodidae
Nec osciinae
Ph ao es lii
ON493672
Phasma odea
Lonchodidae
Lonchodinae
Sungaya inexpec a a
OQ682523
Phasma odea
He e op e ygidae
Ob iminae
Ca ausius sp.
OQ682524
Phasma odea
Lonchodidae
Lonchodinae
Lopaphus albopunc a us
OQ682525
Phasma odea
Lonchodidae
Nec osciinae
Lopaphus sphale us
OQ682526
Phasma odea
Lonchodidae
Nec osciinae
Ma messoidea bispina
OQ682527
Phasma odea
Lonchodidae
Nec osciinae
Ph ao es lianzhouensis
OQ682528
Phasma odea
Lonchodidae
Lonchodinae
Pulch iphyllium biocula um
OQ682529
Phasma odea
Phylliidae
Phylliinae
Pulch iphyllium gigan eum
OQ682530
Phasma odea
Phylliidae
Phylliinae
Ca ausius mo osus
PP230539
Phasma odea
Lonchodidae
Lonchodinae
Neohi asea hongkongensis
PP437079
Phasma odea
Lonchodidae
Nec osciinae
Macellina souchongia
PQ469774
Phasma odea
Phasma idae
Cli umninae
Scep ophasma bi ube cula um
PQ469775
Phasma odea
Phasma idae
Cli umninae
Table S2. Da ashee o Cli umninae used o s anda d molecula ma ke s phylogeny in his s udy.
Species Regions ID 18S 28S H3 COI COII 12S 16S
Da es mu udensis
SB0329 MN925360 MN925489 MN925617 MN925733 MN925858 MN925079 MN925222
Haaniella go ocho i
SB0136 MN925280 MN925418 MN925543 MN925670 MN925784 MN925004 MN925137
Mea nsiana bullosa
SB0287 MN925344 MN925477 MN925604 MN925721 MN925846 MN925064 MN925207
Bacillus a icus a icus Palea c ic AATcug1
AF038226
Bacillus a icus ca ius Palea c ic ACAne 1
AF038228
Bacillus a icus cyp ius Palea c ic ACYepy1
AF038227
Bacillus g andii benazzii Palea c ic GBE be2
AF148315
Bacillus g andii g andii Palea c ic GGRcgc6
AF148313
Bacillus g andii ma e imi Palea c ic GMAma 3
AF038224
Bacillus lynceo um Palea c ic LYNcas1
AF038219
Bacillus whi ei Palea c ic WHIpma2
AF038213
Bacillus a icus Palea c ic BAL4
OK333683 OK314536 OK324157 OK314810
Bacillus ossius Palea c ic BAL3
OK333682 OK314624 OK324156 OK314806
Clonopsis gallica Palea c ic CGAlau1
AF096287
Clonopsis ma occana Palea c ic TAR6
GQ370575
Clonopsis soumiae Palea c ic TAR64
GQ370577
Clonopsis elici a is Palea c ic CLO2
OK314556 OK324158 OK314839
Lep ynia a enua a Palea c ic -APO502
AF241443
Lep ynia cap ai Palea c ic -AMA460
AF241439
Lep ynia mon ana Palea c ic LET1
OK333686 OK314686 OK324162 OK314713
Pijnacke ia o iginis Palea c ic SB0316
OK324238
Pijnacke ia econdi a Palea c ic PVRFAS0001
KT799537 KT799543
Pijnacke ia econdi a Palea c ic PVRFAS0028
KT799542
KT799548
Pijnacke ia hispanica Palea c ic PIJ1
OK333708 OK314673 OK324181 OK314727
Pijnacke ia lelongi Palea c ic HAL386 MW138562 MW138712
MW138481 MW138635
Pijnacke ia o iginis Palea c ic HTIS637 MW138566 MW138715
MW138485 MW138639
Pijnacke ia mase ii Palea c ic HAV100 MW138563
MW138482 MW138636
Pijnacke ia ba ba ae Palea c ic HEP494 MW138564 MW138713
MW138483 MW138637
Macynia labia a A o opical MAC1
OK314657
G a idia sp. 1 A o opical STI11
KT426686 KT426592 KT426631 KT426621
G a idia sp. 2 A o opical STI15
FJ474177 FJ474256 FJ474333 FJ474403
G a idiidae sp. 1 A o opical SB0215
OK333761 OK314625 OK324233 OK314764 OK333598 OK333446
G a idiidae sp. 2 A o opical SB0456 OK333970 OK333809 OK314644 OK324283 OK314721 OK333644 OK333495
G a idiidae sp. 3 A o opical SB0458 OK333971 OK333810 OK314649 OK324284 OK314850 OK333645 OK333496
Phalces ube cula us A o opical PHT1
KT426682
KT426648 KT426619
Zehn ne ia mys ica A o opical ZEM1
FJ474176 FJ474255 FJ474332 FJ474402
Clona ia con o mans O ien al GRC1
FJ474132 FJ474210 FJ474288 FJ474363
Clona ia i zschei O ien al WS021
KJ024562 KJ024500 KJ024453
Clona ia i zschei O ien al SB0497 OK333985 OK333824 OK314684 OK324297
OK333657
Clona ia lue hyi O ien al SB0496 OK333984 OK333823 OK314602 OK324296 OK314769 OK333656 OK333509
Clona ia na alis A o opical WS120 MK291794 MK291869 MK291565
MK291641 MK291716
Clona ia sp. 1 A o opical WS066 MK291769 MK291844 MK291541
MK291616 MK291692
Clona ia sp. 2 A o opical SB0193 MN925307 MN925439 MN925567 MN925693 MN925810 MN925026
Clona ia sp. 3 A o opical SB0212 OK333919 OK333759 OK314639 OK324231 OK314828 OK333596 OK333444
Clona ia sp. 4 A o opical SB0214 OK333920 OK333760 OK314676 OK324232 OK314834 OK333597 OK333445
Clona ia sp. 5 O ien al SB0498 OK333986 OK333825 OK314618 OK324298 OK314820 OK333658 OK333510
Clona ia sp. 6 A o opical SB0511 OK333988 OK333827 OK314641
OK314742
Clona ia sp. 7 A o opical CLN4
OK314678
OK314758
Cuniculina cuniculus O ien al WS146 MK291815 MK291890 MK291584
MK297282 MK291660 MK291735
Cuniculina sp. O ien al SB0318 MN925356 MN925488 MN925615 MN925732 MN925857 MN925076 MN925219
En o ia sp. O ien al SB0355 OK333928
OK314675 OK324243 OK314841 OK333607 OK333453
En o ia koshunensis O ien al ENK1
OK333685 OK314630 OK324161
Lobo emo a bidoupensis O ien al SB0354 OK333927 OK333767 OK314627 OK324242 OK314774 OK333606 OK333452
Lobo emo a schei ei O ien al SB0163 MN925299 MN925432 MN925560
MN925803
MN925156
Lobo emo a schei ei O ien al SB0196 OK333916 OK333756 OK314593 OK324228 OK314857 OK333593 OK333441
Lobo emo a sp. O ien al SB0399 OK333950 OK333789 OK314597 OK324264 OK314747 OK333627 OK333474
Macellina souchongia O ien al GHR005 PQ111436 PQ111451 PQ112637 PQ469775
Macellina den a a O ien al SB0084 MN925261 MN925399 MN925527 MN925656
MN924987 MN925118
Medau a job ensis O ien al SB0378 OK333943 OK333782 OK314576 OK324258 OK314812 OK333621 OK333467
Medau a scab iuscula O ien al SB0352 OK333925
OK314692 OK324240 OK314784 OK333604 OK333450
Medau oidea ex aden a a O ien al WS33 AY121173 AY125313 AY125256
KJ024503 KJ024458
Medau oidea oman ica O ien al SB0353 OK333926 OK333766 OK314574 OK324241 OK314716 OK333605 OK333451
Medau oidea sp. 1 O ien al SB0132 OK333902 OK333740 OK314621 OK324213 OK314726 OK333580 OK333427
Medau oidea sp. 2 O ien al SB0181 OK333912 OK333751 OK314702 OK324223 OK314771 OK333588 OK333437
Medau omo pha oeda a O ien al SB0182 MN925302 MN925435 MN925563 MN925689 MN925806 MN925021 MN925159
Medau omo pha egina O ien al SB0086 OK333892 OK333730 OK314583 OK324203 OK314741 OK333571 OK333418
Pa apachymo pha spinige a O ien al WS080 MK291775 MK291850 MK291547
MK297254 MK291622 MK291698
Pa apachymo pha spinosa O ien al GB109 MK291776 MK291851 MK291548
MK297255 MK291623 MK291699
Pa apachymo pha zomp oi O ien al PAZ1
OK333706 OK314648 OK324179 OK314753
P e ulina dis inc issima O ien al SB0398 OK333949 OK333788 OK314691
OK314796 OK333626 OK333473
Ramulus a emis O ien al WS046 KJ024416.1 KJ024395.1 KJ024532.1
KJ024567.1
KJ024460.1
Ramulus i egula i e den a um Sino-Japanese SB0501
OK314643 OK324299
Ramulus mikado Sino-Japanese SB0370 OK333936 OK333775 OK314539 OK324251 OK314786 OK333614 OK333460
Ramulus nema odes O ien al SB0346 MN925369 MN925497 MN925628 MN925741 MN925865 MN925088 MN925230
Ramulus sp. 1 O ien al SB0347 OK333924 OK333765 OK314668 OK324239 OK314824 OK333603 OK333449
Ramulus sp. 2 O ien al SB0188 OK333914 OK333754 OK314541 OK324226 OK314836 OK333591 OK333440
Ramulus haii O ien al RAT1
FJ474166 FJ474244 FJ474322 FJ474391
Rhamphophasma spinico ne O ien al WS058 MK291764 MK291839 MK291536
MK291687
Scep ophasma bi ube cula um Palea c ic GHR003 PQ111431 PQ111449 PQ112636 PQ469774
Scep ophasma hispidulum O ien al WS27 AY121167 AY125307 AY125250
KJ024563
KJ024455
Scep ophasma langkawicense O ien al WS26 AY121166 AY125306 AY125249
KJ024454
Scep ophasma sp. O ien al SB0517
OK333829 OK314667
Table S3. Bes pa i ioning scheme and bes - i ing models o mi ochond ial genes selec ed by Pa i ionFinde 2 wi h BIC
o mi ogenomes phylogeny analysis.
Subse
Subse pa i ions
Bes Model
P1
nad1_codon1, nad5_codon1, nad4_codon1, nad4L_codon1
GTR+I+G
P2
nad5_codon2, nad4L_codon2, nad1_codon2, nad4_codon2
GTR+I+G
P3
nad5_codon3, nad4L_codon3, nad1_codon3, nad4_codon3
GTR+I+G
P4
cox1_codon1
GTR+I+G
P5
cox1_codon2
GTR+I+G
P6
Cy B_codon3, cox1_codon3
GTR+I+G
P7
a p8_codon1, nad2_codon1, nad6_codon1, nad3_codon1, a p6_codon1
GTR+I+G
P8
a p8_codon2, nad6_codon2, nad2_codon2
GTR+I+G
P9
nad2_codon3, a p8_codon3, cox3_codon3, a p6_codon3, cox2_codon3, nad3_codon3, nad6_codon3
HKY+I+G
P10
Cy B_codon1, cox2_codon1, cox3_codon1
GTR+I+G
P11
nad3_codon2, a p6_codon2, Cy B_codon2, cox2_codon2, cox3_codon2
GTR+I+G
P12
nL
GTR+I+G
P13
nS
GTR+I+G
Table S4. Bes pa i ioning scheme and bes - i ing models o s anda d molecula ma ke s selec ed by Pa i ionFinde 2
wi h AICc o 7 s anda d molecula ma ke s phylogeny analysis.
Subse
Subse pa i ions
Bes Model
P1
COX1_codon1
GTR+I+G
P2
COX1_codon2
TIM+I+G
P3
COX1_codon3, COX2_codon3
GTR+I+G
P4
COX2_codon1
GTR+I+G
P5
COX2_codon2
TVM+I+G
P6
H3_codon1
TIM+G
P7
H3_codon2
JC+I
P8
H3_codon3
TRNEF+G
P9
12S
GTR+I+G
P10
16S
GTR+I+G
P11
18S
GTR+I+G
P12
28S
GTR+I+G
Table S5. Gene o de and o ganiza ion o he mi ochond ial genomes o Scep ophasma bi ube cula um
(Sb) and Macellina souchongia (Ms).
Gene S and
Leng h (bp)
Sb/Ms
nI
o wa d
66/68
nQ
e e se
69/69
nM
o wa d
67/66
nad2
o wa d
1017/999
nW
o wa d
65/65
nC
e e se
64/64
nY
o wa d
68/66
cox1
o wa d
1539/1539
nL
o wa d
64/64
cox2
o wa d
687/705
nK
o wa d
70/70
nD
o wa d
66/65
a p8
o wa d
156/156
a p6
o wa d
678/678
cox3
o wa d
789/789
nG
o wa d
63/63
nad3
o wa d
366/366
nA
o wa d
64/65
nR
o wa d
64/64
nN
o wa d
65/65
nS
o wa d
68/68
nE
o wa d
66/66
nF
e e se
65/66
nad5
e e se
1713/1713
nH
e e se
63/63
nad4
e e se
1332/1332
nad4l
e e se
285/273
nT
o wa d
63/65
nP
e e se
67/64
nad6
o wa d
474/474
cob
o wa d
1134/1134
nS
o wa d
69/67
nad1
e e se
958/966
nL
e e se
66/67
nL
o wa d
1237/1266
nV
e e se
68/69
nS
o wa d
758/760
con ol egion
o wa d
3025/1686
