In e na ional Con e ence on Bio ab ica ion 2023, Sep embe 17 h -20 h 2023, Saska oon, Canada
Tissue Sca olds Cha ac e iza ion Using
Synch o on Radia ion Mic o-Compu ed
Tomog aphy wi h Helical Acquisi ion Mode
Xiaoman Duan1, Xiao Fan Ding1, Nai ao Li1, Ning Zhu1, 2, 3, Xiongbiao Chen1, 4
1Di ision o Biomedical Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada.
2Depa men o Chemical and Biological Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada
3Canadian Ligh Sou ce, Saska oon, S7N 2V3, SK, Canada
4Depa men o Mechanical Enginee ing, Uni e si y o Saska chewan, Saska oon, SK S7N 5A9, Canada
Keywo ds — Tissue enginee ing, Hyd ogel sca old, Cha ac e iza ion, Mic o-compu ed omog aphy.
INTRODUCTION
In he ield o issue enginee ing, hyd ogel sca olds ha e
gained signi ican a en ion due o hei unique p ope ies,
due o hei unique p ope ies. Accu a e imaging echniques
a e essen ial o s udying he in e nal s uc u e and p ope ies
o hese sca olds. Hyd ogel sca olds ha e e y low densi y
and synch o on adia ion mic o-compu ed omog aphy (SR-
µCT) shows high con as wi h h ee-dimensional and non-
in asi e cha ac e iza ion [1].
Despi e many ad an ages, SR-µCT image quali y o
hyd ogel s ill needs o be imp o ed due o common ing
a i ac s esul ed om sys ema ic e o s o de ec s on he
scin illa o , monoch oma o , o il e s. Such a i ac s usually
educe he accu acy when isualizing and cha ac e ing
samples. Me hods ha e been de eloped o educe he ing
a i ac s, e.g., low-pass il e ing algo i hm, bu hese
app oaches su e om limi a ions. This wo k in eg a es SR-
µCT wi h he helical acquisi ion mode (SR-µHCT) o a oid
he ing a i ac s issues. SR-µHCT in ol es wo mo ions, a
ho izon al o a ion and a e ical mo ion which can sp ead
he in ensi y o ing a i ac s o e la ge egions in he
e ical di ec ion, he e o e educing he e ec s o a i ac s.
MATERIALS AND METHODS
4% w/ algina e we e p in ed o sca olds (dimension: 2 × 2
× 10 mm3) wi h he needle’s diame e o 100 μm. A e 15-
minu e c oss-linking, he algina e sca olds we e inse ed in o
PCL ubes which can p o ide mechanical suppo [2]. The
assembled condui s la e we e implan ed in o inju ed scia ic
ne e o Sp ague−Dawley a s. Eu hanasia was conduc ed 2
days a e su ge y and he whole hindlimb was collec ed and
was ixed wi h o malin o imaging (ex i o).
The SR-µHCT imaging we e pe o med a he BMIT-ID
beamline, Canadian Ligh Sou ce (CLS), Canada. All scans
we e pe o med a sample- o-de ec o dis ance o 1.5 m,
pho on ene gy o 30 keV, helical pi ch o 1.5, and he pixel
size o 13 µm.
RESULTS AND DISCUSSION
Fig. 1 shows image quali y compa isons o ex i o sca olds
sample wi h SR-µCT and SR-µHCT. While SR-µCT exhibi s
adequa e image con as , i is suscep ible o ing a i ac s ha
a ec he o e all quali y. Al hough hese a i ac s can be
mi iga ed using a low-pass il e ing me hod, i leads o he
in oduc ion o addi ional ing-like shadows. On he o he
hand, SR-µHCT e ec i ely minimizes ing a i ac s wi hou
in oducing any u he dis o ions o a i ac s. Quan i a i e
e alua ion index, con as - o-noise a io (CNR) and signal-
o-noise a io (SNR) we e also measu ed. These esul s
illus a e a highe cha ac e iza ion accu acy o SR-µHCT
compa ed wi h SR-µCT, which is essen ial o ollowing
quan i a i e analysis, e.g., segmen a ion.
Fig. 1 Ex i o hyd ogel sca olds imaging wi h SR-µCT and SR-µHCT;
(A1-C1) SR-µCT image, SR-µCT image p ocessed by a ing a i ac s
emo al algo i hm, and SR-µHCT image. (A2-C2) Co esponding enla ged
egions o in e es a posi ions indica ed by yellow ec angula .
ACKNOWLEDGEMENTS
This wo k is suppo ed by Na u al Sciences and Enginee -
ing Resea ch Council o Canada (G an numbe s: RGPIN
06007-2019 and RGPIN 06396-2019).
REFERENCES
[1] X. Duan e al., Tissue Enginee ing Pa C: Me hods, ol. 27, no.
11, pp. 573-588, 2021.
[2] L. Ning e al., ACS Appl. Ma e . In e aces, ol. 13, no. 22, pp.
25611-25623, 2021.
