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Midterm course to Research Data Management for TargetRNA

Author: Bösl, Korbinian
Publisher: Zenodo
DOI: 10.5281/zenodo.17734587
Source: https://zenodo.org/records/17734587/files/2025-11-27_targetRNA_midterm_RDM.pdf
Mid e m upda e o you DMP
Ko binian Bösl
Da a managemen coo dina o
Cen e o Digi al Li e & ELIXIR No way
27 No embe 2025
Objec i es
? Ques ions
●How do you backup up you da a?
●How can I make su e da a is unal e ed by s o age o ans e ?
●How can I documen my da a?
! Objec i es
●Check in and upda e you DMP
●Iden i y po en ial gaps in you RDM s a egy
knowledgebase.n di4chem.de/knowledge_base
dmki .elixi -eu ope.o g
Backup

Backup
A oid losing da a!
Use (ins i u ionally) managed s o age wi h
au oma ic backup (and snapsho s?)
OneD i e/Sha epoin @ UiB
A oid: da a solely sa ed o lap ops,
s and-alone HDDs, humb d i es, …
In gene al
3 - 2 -1 ule
3 copies
2 diffe en media
1 off-si e backup
5min o check he backup s a us o you da a
Checksums
Checksum you da a o de ec co up ion du ing s o age o ans e
MD5sum in buil in e e y OS
windows powe shell: $ ce u il -hashfile [filename] MD5
Mac OS/nix: $ md5sum [filename(s)|*] > checksums.md5
$ md5sum -c checksums.md5
Fo secu i y c i ical o legally ele an : sha256
Demo + 5 min o you o checksum one o you da a olde s
Gene: PIK3CA
Sequence iden i ie s:
Figu e 6 om Zhao, S., and Zhang, B. (2015). BMC Genomics 16 licensed unde C ea i e Commons A ibu ion 4.0 In e na ional License
README files
S udy/p ojec le el README
This README ile was gene a ed on [YYYY-MM-DD] by [NAME]
GENERAL INFORMATION
- S udy/p ojec i le:
- Desc ip ion: <p o ide a sho desc ip ion o he s udy/p ojec >
- P inciple In es iga o :
- Link o Da a managemen plan
ORGANIZATION
- Folde s uc u e: simila o olde s uc u e example abo e (below)
- File naming con en ions (wi h examples) <unless you p ojec is big and
you ha e README iles in e e y sub olde wi h his in o ma ion p o ided
he e>
- File o ma s: <P o ide a lis o all ile o ma s p esen in his s udy/p ojec >
Da a le el README
GENERAL INFORMATION
- Da ase i le:
- Desc ip ion: <p o ide desc ip ion o he da ase o igin, s eps used in i s
gene a ion, con en and i s pu pose>
ORGANIZATION
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- File naming con en ions: <p o ide explana ion o he elemen s used,
allowed alues and examples>
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[...]
15 min o you o c ea e a README o one o you da a olde s
RDMki -> you ask -> da a
documen a ion

Minimum In o ma ion abou a high- h oughpu
Nucleo ide SeQuencing Expe imen (MINSEQE)
Repo ing s anda ds o e.g. RNA-Seq
In o ma ion ega ding submission on A ayExp ess
h ps://www.ebi.ac.uk/a ayexp ess/submi /o e iew.h ml
MINSEQE en y on FAIRha ing:
h ps:// ai sha ing.o g/bsg-s000174/
h ps://www. ged.o g/p ojec s/minseqe/
Requi ed o deposi ion o sequencing da a on A ayExp ess
MINSEQE
Example
Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
MINSEQE
Type o me ada a
Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
Expe imen al Condi ions/Design
P o ocols:
●T ea men
●Sample Collec ion
●G ow h
●Nucleic Acid Ex ac ion
●Lib a y Cons uc ion
●Sequencing P o ocol

Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
Expe imen al Condi ions/Design
P o ocols:
●T ea men
●Sample Collec ion
●G ow h
●Nucleic Acid Ex ac ion
●Lib a y Cons uc ion
●Sequencing P o ocol
High- h oughpu Sequence Alignmen P o ocol
Da a No maliza ion P o ocol
Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
Expe imen al Condi ions/Design
P o ocols:
●T ea men
●Sample Collec ion
●G ow h
●Nucleic Acid Ex ac ion
●Lib a y Cons uc ion
●Sequencing P o ocol
High- h oughpu Sequence Alignmen P o ocol
Da a No maliza ion P o ocol
Ensembl ID
Re Seq ID
as q
bam
HTML
cs / s
Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
Expe imen al Condi ions/Design
P o ocols
High- h oughpu Sequence Alignmen P o ocol
Da a No maliza ion P o ocol
as q
bam
cs / s
Ensembl ID
Re Seq ID
Taxon
Adminis a i e In o ma ion:
Pe son(s)
O ganisa ions
Publica ions
Expe imen al Condi ions/Design
P o ocols
High- h oughpu Sequence Alignmen P o ocol
Da a No maliza ion P o ocol
as q
bam
cs / s
Ensembl ID
Re Seq ID
Taxon
Pa en s in Eu ope
Ac i e p ocess
No p io disclosu e
Apply only in limi ed geog aphical scopes
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Does no apply o ac s
Economic igh s s. mo al igh s
Au ho li e ime + 70 yea s
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Defined addi ional (sui gene is) IPR o :
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no all unde copy igh ] a anged in a sys ema ic o me hodical way and
indi idually accessible by elec onic o o he means”
15 yea s by de aul
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CC-BY-SA 3.0 License - Wikipedia
Which o hese is de i a i e wo k?
Download some code and use one o he unc ions in you code
Linking o lib a ies (s a ic o dynamic), plug-ins, and d i e s
Clean oom design (you ne e see he code)
Ex ending code you go om somewhe e
Rew i ing code o a diffe en p og amming language
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❌Linking o lib a ies (s a ic o dynamic), plug-ins, and d i e s
❌Clean oom design (you ne e see he code)
✔Ex ending code you go om somewhe e
✔Rew i ing code o a diffe en p og amming language
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Inc ease o willingness o euse ou pu s (Reusabili y)
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Challenges
Mul iple A ibu ions o se e al sou ces (license s acking)
Mul iple incompa ible sou ce licenses
Legal comme cial defini ion (e.g. use by jou nals)
CC-BY 4.0 license symbols by h ps://c ea i ecommons.o g/
Challenges
Mul iple A ibu ions o se e al sou ces (license s acking)
Mul iple incompa ible sou ce licenses
Legal comme cial defini ion (e.g. use by jou nals)
Unin en ional es ic i e

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Yes!
F ee So wa e Founda ion, Inc. . Cisco Sys ems, Inc., 2009
…
Busybox GPL lawsui s 2007-2009
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Digi al Li e 2021: Know-how wo kshop on IPR in digi al bio ech
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You asks: Licensing | RDMki
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