E ec o empe a u e and ood concen a ion on he
ela ionship be ween g ow h and AARS ac i i y in
Pa aca ia g ani nauplii
1Ins i u e o Oceanog aphy and Global Change
2Ins i u o Español de Oceanog a ía
Inma He e a1, Lidia Yeb a2and San iago He nández-Léon1
E ec o de la empe a u a y de la concen ación de alimen o en la elación
en e el c ecimien o somá ico y la ac i idad de la enzima aminoacil ARN
sin e asa en nauplios de Pa aca ia g ani
Pa aca ia g ani (Sa s, G.O. 1904)
Typical o coas al a ea
Condi ioned:
Physical ac o s ( empe a u e)
Biological ac o s ( ood)
Aca iidae a e common in coas al and
es ua ine habi a s wo ldwide.
An impo an componen in he die o
a many o plank on species.
OBJECTIVE
The ela ionship be ween di ec g ow h a es and speci ic
AARSsi u ac i i ies o alida e he AARS me hod as g ow h
index o his species unde di e en empe a u es (12-28ºC)
and ood quan i y (0-880 µg C·L-1).
The e ec o empe a u e and ood quan i y on he g ow h o
Pa aca ia g ani (Sa s 1904).
METHODOLOGY
Oxy his ma ina
ICM
Ins i u e o
Ma ine
Sciences
Pa aca ia g ani
12h day: 12h nigh
pho ope iod
Rhodomonas bal ica
/2 medium
24 hou s Eggs we e collec ed and p ese ed in he idge (4ºC)
METHODOLOGY
Expe imen s a di e en empe a u es
12 º C 16 º C 19.8 º C
24 º C 26 º C 28 º C
Eggs addi ion:
(22.000-64.548)
16 hou s
Oxy his ma ina
220-286 μgC·L-1
(~2 nauplii mL-1)

METHODOLOGY
Expe imen s unde di e en ood concen a ions
0µg C·L
-1 11 µg C·L-1 55 µg C·L-1 110 µg C·L-1
220 µg C·L-1 440 µg C·L-1 880 µg C·L-1
(~2 nauplii mL-1)
METHODOLOGY
100 ml nauplii cul u e
Fixed wi h Lugol’s acid (4%)
Measu ed o leng h and abundance
Mul isize Coul e Coun e
Nauplii g ow h expe imen s
Con e ed in ca bon (C) assuming a ca bon/d y
weigh a io o 0.40 (Pos el e al. 2000).
Leng h measu emen and weigh -speci ic g ow h calcula ions
D y weigh (dw) / leng h
Du bin and Du bin (1978)
A.clausi
W = 19.04 L 2.849, = 0.99
W = d y weigh (μg)
L = p osome leng h(mm)
Tempe a u e quo ien (Q10)
R1and R2= G ow h a es
T1and T2= Tempe a u es
P o ein syn hesis
Aminoacids
mRNA
Ribosome
Ribosome eading a RNA chain p io o he syn hesis o a p o ein (Whi esides, 2001).
P o ein
Fig 2. Pa aca ia g ani nauplii. E ec o empe a u e on A) g ow h (d-1), B) spAARSs(nmPPi·mg p o -1·h-1),
C) indi idual AARSs(nmPPi·ind-1·h-1).
RESULTS & DISCUSSION
Tempe a u e (ºC)
10 14 18 22 26 30
Indi idual AARSs
(nmPPi · ind-1 · h-1)
0.000
0.005
0.010
0.015
0.020
0.025
10 14 18 22 26 30
G ow h (d-1)
0.0
0.2
0.4
0.6
0.8
1.0
10 14 18 22 26 30
spAARSs
(nmPPi · mg p o -1 · h-1)
20
40
60
80
100
120
G = -0.188 + 0.038 · T
2 = 0.984, p < 0.001
spAARSs= -42.51 + 5.44 · T
2 = 0.942, p < 0.001
AARSs·ind-1 = -0.010 + 0.001 · T
2 = 0.852, p < 0.001
Q10 2.1
Q10 2.1
Q10 2.4

RESULTS & DISCUSSION
Fig 5. Pa aca ia g ani nauplii. Rela ionship
be ween g ow h a es (d-1) and A) speci ic AARSs
ac i i ies (nmPPi·mg p o -1·h-1), B) indi idual AARSs
(nmPPi·ind-1·h-1) a di e en empe a u es (ºC).
spAARSs (nmPPi·mg p o -1·h-1)
20 40 60 80 100 120
-0.1
0.1
0.3
0.5
0.7
0.9
Indi idual AARSs (nmPPi·ind-1·h-1)
0.000 0.005 0.010 0.015 0.020 0.025
-0.1
0.1
0.3
0.5
0.7
0.9
G ow h (d-1)
A
B
G = 0.25 + 31.65 · AARSs·ind-1
2 = 0.833, p < 0.001
G = 0.13 + 0.007 · spAARSs
2 = 0.945, p < 0.001
Yeb a e al., 2005
Calanus helgolandicus
R2=0.55
Yeb a e al., 2006
Calanus inma chicus
R2= 0.55
RESULTS & DISCUSSION
Days a e ha ching
ln weigh (ng C)
0
µ
g
C·L
-1
012345678
2
3
4
5
6
11
µ
g
C·L
-1
012345678
2
3
4
5
6
55
µ
g
C·L
-1
012345678
2
3
4
5
6
110
µ
g
C·L
-1
012345678
2
3
4
5
6
220
µ
g
C·L
-1
012345678
2
3
4
5
6
440
µ
g
C·L
-1
012345678
2
3
4
5
6
880
µ
g
C·L
-1
012345678
2
3
4
5
6
-0.01 - 0.68 d-1
Fig 3. Pa aca ia g ani nauplii. Ca bon con en (ng C) inc eases unde di e en ood concen a ions.
Food concen a ion (µg C·l-1)
0 200 400 600 800 1000
Indi idual AARSs
(nmPPi · ind-1 · h-1)
0.000
0.002
0.004
0.006
0.008
0 200 400 600 800 1000
G ow h (d-1)
-0.1
0.1
0.3
0.5
0.7
0.9
0 200 400 600 800 1000
spAARSs
(nmPPi · mg p o -1 · h-1)
20
30
40
50
60
70
80
RESULTS & DISCUSSION
Fig 4. Pa aca ia g ani nauplii. E ec o ood concen a ion on A) g ow h (d-1), B) spAARSs(nmPPi·mg p o -
1·h-1), C) indi idual AARSs(nmPPi·ind-1·h-1); open ci cle: alue no included in i (see ex ).
G = 0.65 · (1 – e(–0.013·C))
2 = 0.986, p < 0.001
AARSs·ind-1 = 0.0032 + 0.0004 ln(C)
2= 0.907, p < 0.05
RESULTS & DISCUSSION
Fig 6. Pa aca ia g ani nauplii. Rela ionship
be ween g ow h a es (d-1) and A) speci ic AARSs
ac i i ies (nmPPi·mg p o -1·h-1), B) indi idual
AARSs(nmPPi·ind-1·h-1) unde di e en ood
concen a ions (µg C·L-1); open ci cle: alue no
included in i (see ex ).
spAARSs (nmPPi · mg p o -1 · h-1)
20 30 40 50 60 70
-0.1
0.1
0.3
0.5
0.7
0.9
Indi idual AARSs (nmPPi · ind-1 · h-1)
0.000 0.002 0.004 0.006
-0.1
0.1
0.3
0.5
0.7
0.9
G ow h (d-1)
A
BG = -0.59 + 204.46 · AARSs·ind-1
2 = 0.951, p < 0.001
G = 1.49 - 0.025 · spAARSs
2 = 0.958, p < 0.0001
RESULTS & DISCUSSION
Fig 7. Pa aca ia g ani nauplii. A) Rela ionship be ween speci ic AARSsac i i ies (nmPPi·mg p o -1·h-1)and
indi idual biomass (µg p o eins · ind-1); B) Rela ionships be ween daily g ow h a es (d-1)andspeci ic
AARSsac i i ies (nmPPi·mg p o -1·h-1) unde di e en ood concen a ions.
20 30 40 50 60 70 80 90
spAARS
s
(nmPPi · mg p o
-1
· h
-1
)
-0.2
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
Daily g ow h a e
s a a ion le el
in e media e le el
sa u a ion le el
0.00 0.05 0.10 0.15 0.20 0.25 0.30
µg p o eins·ind
-1
0
10
20
30
40
50
60
70
80
90
spAARSs (nmPPi·mg p o -1·h-1)
spAARSs= 64.3 – 165.0 · ind biomass
2 = 0.490, p < 0.0001

CONCLUSIONS
G ow h and p o ein syn hesis a es o Pa aca ia g ani nauplii depended on empe a u e
and ood concen a ion.
AARS ac i i y is alid as index o soma ic g ow h o P. g ani nauplii when g ow h is no
limi ed by ood a ailabili y.
The esul s p esen ed he e add o p e ious s udies showing ha he AARSsac i i y is a
use ul ool o es ima ing soma ic g ow h in copepods.
ACKNOWLEDGMENTS
Zooplank on Ecology G oup a he Ins i u e o Ma ine
Sciences (ICM, Ba celona, Spain)
Oi hona p ojec (CTM2007-60052) and Luci e p ojec (CTM2008-03538/MAR)
Thank you o you a en ion!
[email p o ec ed]
Inma He e a