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Deep Learning based Detection, Segmentation and Counting of Benthic Megafauna in Unconstrained Underwater Environments

Abstract

Assessing and monitoring benthic communities is increasingly important in view of global alteration of marine environments. Deep learning has proven to effectively detect marine specimen in underwater imagery but still face problems with small input datasets, unconstrained environments and class imbalance. This study evaluates a data augmentation strategy to alleviate these limitations. Through synthetically derived image compositions, the entire input dataset was greatly extended from 700 to 12700 images. Additionally, specimen numbers of brittle stars, soft corals and glass sponges are equalized resulting in a mean average precision increase of 24 %. The overall mean average precision for box detections yields 76.7 and for instance segmentation 67.7 at an intersection over union threshold of 0.5. This study shows that deep architectures such as the deployed CenterMask via ResNeXt-101 model can successfully be trained with few original images from varying underwater scenes.

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Deep Learning based Detection, Segmentation and Counting of Benthic Megafauna in Unconstrained Underwater Environments

Author: Lütjens, Mona Caroline,Sternberg, Harald
Publisher: Elsevier
DOI: 10.1016/j.ifacol.2021.10.076
Source: https://repos.hcu-hamburg.de/bitstream/hcu/664/1/1-s2.0-S2405896321014786-main.pdf
IFAC Pape sOnLine 54-16 (2021) 76–82
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10.1016/j.i acol.2021.10.076
10.1016/j.i acol.2021.10.076 2405-8963
Copy igh ©
2021 The Au ho s. This is an open access a icle unde he CC BY-NC-ND license
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)
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Mona Lü jens e al. / IFAC Pape sOnLine 54-16 (2021) 76–82 77
Copy igh ©
2021 The Au ho s. This is an open access a icle unde he CC BY-NC-ND license
(
h ps://c ea i ecommons.o g/licenses/by-nc-nd/4.0/
)
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
Deep Lea ning based De ec ion, Segmen a ion and Coun ing o Ben hic
Mega auna in Uncons ained Unde wa e En i onmen s
Mona Lü jens* Ha ald S e nbe g*
*Ha enCi y Uni e si y Hambu g, Depa men o Hyd og aphy and Geodesy,
Henning-Vosche au-Pla z 1, 20457 Hambu g, Ge many
(e-mail: {mona.lue jens,ha ald.s e nbe g}@hcu-hambu g.de)
Abs ac : Assessing and moni o ing ben hic communi ies is inc easingly impo an in iew o global
al e a ion o ma ine en i onmen s. Deep lea ning has p o en o e ec i ely de ec ma ine specimen in
unde wa e image y bu s ill ace p oblems wi h small inpu da ase s, uncons ained en i onmen s and class
imbalance. This s udy e alua es a da a augmen a ion s a egy o alle ia e hese limi a ions. Th ough
syn he ically de i ed image composi ions, he en i e inpu da ase was g ea ly ex ended om 700 o 12700
images. Addi ionally, specimen numbe s o b i le s a s, so co als and glass sponges a e equalized
esul ing in a mean a e age p ecision inc ease o 24 %. The o e all mean a e age p ecision o box
de ec ions yields 76.7 and o ins ance segmen a ion 67.7 a an in e sec ion o e union h eshold o 0.5.
This s udy shows ha deep a chi ec u es such as he deployed Cen e Mask ia ResNeX -101 model can
success ully be ained wi h ew o iginal images om a ying unde wa e scenes.
Keywo ds: objec de ec ion, deep lea ning, da a augmen a ion, ma ine image y, ben hic mega auna
1. INTRODUCTION
Global al e a ion o ma ine en i onmen s due o o e ishing,
pollu ion, physical habi a des uc ion and clima e change ha e
led o an inc easing decline o animal di e si y and abundance
h oughou many ma ine ecosys ems (Jackson, 2008).
Especially ben hic mega auna in he Sou he n Ocean a e a isk
o en i onmen al change and a e o signi ican ecological
alue as hey al e small-scale opog aphy o seabed habi a s
a ec ing he en i e ben hic communi y (Gili e al., 2006).
Assessing he biodi e si y and cha ac e isa ion o ben hic
communi ies is inc easingly impo an o iden i ying
ulne able ma ine ecosys ems and de eloping conse a ion
s a egies.
Ma ine habi a s ha e been s udied based on mainly h ee
p ac ices: physical sampling me hods using sledges o
awling, acous ical echniques and op ical sys ems. While
physical me hods a e able o collec specimen on a lowe
axonomical scale hey des uc he en i onmen and hei
sampling a e is a he low. Op ical sys ems a e mo e cos
e ec i e, obus and p ecise han acous ic sys ems which led
o a la ge g owing lib a y o digi al unde wa e images
h oughou ecen yea s pa ing he way o new esea ch in
au oma ic analy ical me hods.
Objec de ec ion, classi ica ion and segmen a ion o image y
has become a subs an ial ask in he ield o compu e ision.
P io adi ional ea u e desc ip o s a e o en used o ex ac
colou , shape o ex u e in o ma ion and a e good a de ec ing
speci ic single objec s such as scallops (Dawkins e al., 2013)
o lobs e s using classi ie s such as suppo ec o machine
(Tan e al., 2018). Howe e , hey a e no obus o a ying
unde wa e scenes ha a e exposed o ma ine snow, wa e
u bidi y, lens dis o ion, spa se, uns able illumina ion and
colou shi due o he su ey pla o m’s a ia ion in speed,
angle o al i ude. Mo eo e , he e is a high a iabili y and
in a iabili y be ween ea u es belonging o he same class and
di e en classes, espec i ely (Pa oni e al., 2021).
Deep lea ning (DL) using con olu ional neu al ne wo ks ha e
p o en o ou pe o m adi ional based objec de ec ion
(Gonzalez-Cid e al., 2017) as hey a e mo e in a ian o he
de o ma ion o images. Addi ionally, images can di ec ly be
used as inpu wi hou he necessi y o p e-p ocessing. Be e
esul s a e o en achie ed using deepe laye s hus inc easing
he numbe o pa ame e s o se e al millions. To achie e high
pe o mances wi h hose models using images o
uncons ained unde wa e scenes o ac oss a ying pla o ms,
a la ge aining da ase size is he mos c ucial pa
(Langenkämpe e al., 2020), o en, howe e , e y ime-
consuming and cos ly o es ablish.
This pape in es iga es he e ec o inpu image da a
augmen a ion and composi ion s a egies in an a emp o
o e come limi a ions o la ge da a se size and class
imbalance. Taking accoun o he esul s, he s a e-o - he a
ancho - ee objec de ec ion and ins ance segmen a ion model
Cen e Mask (Lee and Pa k, 2019) ia ResNeX -101 (Xie e al.,
2017) will be ained based on a small, highly di e se 700
image da ase . In his wo k, he de ec ion, segmen a ion and
coun ing o glass sponges (hexac inellids), so co als
(p imnoids and ch ysogo giids) and b i le s a s (ophiu oids)
will be assessed p o iding i s s eps owa ds u u e abundance
and size es ima ions o hese specimen.
2. RELATED RESEARCH
Se e al p e ious wo ks add ess objec de ec ion and
classi ica ion o ma ine scenes using cu ing-edge DL
a chi ec u es such as Re inaNe wi h ResNe -50 (Boulais e al.,
2020) o FDCNe (Lu e al., 2018) showing good classi ica ion
esul s on single-labelled o iconic images. Au oma ic
segmen a ion o ben hic auna has been s udied o co als
using DeepLab 3+ (Pa oni e al., 2021) and scale wo ms
using U-Ne and VGG-16 CNN (Shashidha a e al., 2020).
Secu ing enough aining da a o DL is c ucial as desc ibed
abo e hence da a augmen a ion echniques a e widely applied.
F equen ly used echniques include he change o ligh
in ensi y, sha pness, noise and blu ing (Salman e al., 2016)
o change o pe spec i e (Huang e al., 2019). Also, o a ion
and c opping o unde wa e images ha e been used
(Langenkämpe e al., 2020). These me hods ha e p o en o
be use ul, howe e , only limi ed image manipula ions can be
pe o med. Mos no ably, he numbe o o iginal anno a ed
images om mos s a ed wo ks exceed he amoun o a ailable
aining da a o his esea ch. The e o e, ano he echnique
will be used which changes he en i e image composi ion and
adds addi ional al e a ion o syn he ically gene a ed images.
Also, no esea ch ega ding ins ance segmen a ion and
coun ing o he selec ed mo pho ypes is known o he au ho s.
3. MATERIALS AND METHODS
3.1 Unde wa e Image Da ase
The image da ase used o his s udy was collec ed du ing he
expedi ion PS118 o he esea ch essel RV Pola s e n in 2019
(Pu se e al., 2021). Images we e sampled using he owed
Ocean Floo Obse a ion and Ba hyme y Sys em (Pu se e
al., 2019) wi h a lying al i ude o app oxima ely 1.5 – 2.5 m
abo e he sea loo . Se en di e en sampling s a ions om he
wes e n Weddell Sea con inen al shel o he no he n Powell
Basin we e selec ed. Each s a ion a ea ea u es di e en
subs a e ypes anging om so and ine mud sedimen o
pebbles and complex ocky opog aphy. The o iginal 3840 x
5760 sized images we e iled o 1440 x 960 o main ain
esolu ion bu educe he need o compu ing powe . 1000
images we e selec ed and anno a ed using he web-based
image segmen a ion ool COCO Anno a o (B ooks, 2019). O
he 1000 images, 700 we e used as aining se , 100 as
alida ion se and 200 as es se . In o al, 3550 anno a ions o
he aining se we e made, o which 87 % belong o he class
b i le s a s, 8 % o he class glass sponges and 5 % o he class
so co als. Fo he es and alida ion se 85 % and 84 % o
he anno a ions belong o he class b i le s a s, 10 % and
12 % o he class glass sponges and 5 % and 4 % o he class
so co als, espec i ely. I is appa en ha he e is a high class
imbalance.
3.2 Da a Augmen a ion
To inc ease he numbe o images o aining, he image
gene a o COCO Syn h (Kelly, 2019) was u ilised which
composes cu ou o eg ound images o objec s o e andom
image backg ounds. Fo eg ounds a e andomly al e ed in
scale, amoun , o a ion and b igh ness o each composi ion
(Figu e 1). Fo his s udy, 30 o eg ounds pe class and 30
backg ounds om o iginal images we e used o aining.
Images o he composi ions we e selec ed om a ying
s a ions and di e om p e ious ones in 3.1. O e all, 12,000
syn he ic images we e deployed o aining o which 2000
images we e gene a ed o glass sponges and so co als each,
o educe he e ec o class imbalance. Now, 33 % o he o al
anno a ions belong o he class glass sponges, 33 % o he class
so co als and 34 % o he class b i le s a s. In o de o
emphasize he selec ed augmen a ion me hod, se e al
equen ly used image manipula ion echniques we e
addi ionally pe o med and compa ed o he selec ed me hod.
The ollowing image a ibu es we e al e ed: b igh ness, colou
one, con as , sa u a ion, sha pness and blu (Figu e 1). In
o al, en di e en al e a ions pe image we e conduc ed.
Fig. 1. Example images o syn he ically de i ed image composi ions (1s ow) and adi ional da a augmen a ion me hods (2nd
ow, om le o igh ): o iginal image, blu , low b igh ness, high b igh ness, blue colou , g een colou , high con as , low
con as , high sa u a ion, low sa u a ion, sha pness
78 Mona Lü jens e al. / IFAC Pape sOnLine 54-16 (2021) 76–82
3.3 Deep Lea ning A chi ec u e and T aining
The deep lea ning a chi ec u e chosen o his s udy is he
ancho - ee one s age ins ance segmen a ion and objec
de ec o Cen e Mask (Lee and Pa k, 2019) in combina ion
wi h he backbone ne wo k ResNeX -101 (Xie e al., 2017).
While objec de ec ion is he cen al ask o abundance and
assemblage s udies, p edic ing masks will be impo an o
u u e size es ima ions and biomass p edic ions o ben hic
species. An ins ance segmen a ion model was he e o e chosen
in o de o adap o di e se esea ch ques ions o u u e
analyses. Also, he model should ope a e on high in e ence
speed while main aining s ong pe o mances o di ec ly
e alua e da ase s on boa d o esea ch essels o s a egic da a
collec ion. Since bo h selec ed a chi ec u es mee he
men ioned equi emen s and u he p oduce excellen esul s
in ecen benchma k challenges such as COCO (Lin e al.,
2014), hey a e an app op ia e choice o he espec i e
compu e ision ask o ma ine imaging.
The backbone ResNeX -101 used o ea u e ex ac ion is an
ad ancemen o he deep esidual ne wo k ResNe (He e al.,
2016) ha has been ecen ly p oposed. Based on ResNe ,
ResNeX -101 ollows he s a egy o epea ing laye s bu
s acks hem pa allel a he han sequen ially. Thus, esul ing in
accu acy imp o emen s while educing he ne wo k
complexi y and numbe o pa ame e s. Fo his s udy he 101
laye ed ne wo k was used.
As a one s age de ec o , Cen e Mask does no ha e a p oposal
s ep and p io i izes in e ence speed. Addi ionally, as an
ancho - ee de ec o , i does no use p ede ined bounding
boxes o iden i y objec s and is hus insensi i e o di e en
da ase s and hype -pa ame e s (e.g. inpu size, scales, e c.).
Hence, ancho - ee de ec o s alle ia e limi a ions o objec s
ha ha e la ge shape a ia ions and a e a he small (Tian e
al., 2019) which is ideal o he selec ed classes used in his
esea ch. Cen e Mask adop s FCOS (Tian e al., 2019) as
de ec ion head ha di ec ly compu es a 4D ec o and a class
label a each p oposed loca ion o di e en le els o ea u e
maps. Then, he spa ial a en ion-guided mask (SAG-Mask)
compu es he segmen a ion masks on each p edic ed box
egion using he spa ial a en ion module (SAM) ha helps he
mask o ocus on signi ican pixels (Lee and Pa k, 2019).
T aining was execu ed on a 64-bi Linux machine equipped
wi h an In el® Xeon® Gold 6254 CPU @ 3.10 GHz and 5
NVIDIA® Tesla® V100 GPU. The base lea ning a e was se o
0.002 and educed by a ac o o 10 a e 25400 and again a e
38100 i e a ions. To educe ea ly o e i ing on highly
di e en ia ed da ase s, he lea ning a e was also educed o
he i s 5080 i e a ions. Addi ionally, a weigh decay was
implemen ed. The maximum numbe o i e a ions was 50800
which co esponds o 20 epochs. All backbone models a e
ini ialized by ImageNe p e- ained weigh s.
3.4 E alua ion P o ocol
To assess he pe o mance o he model, he e alua ion me ics
a e age p ecision, a e age ecall, F1 measu e and accu acy a e
u ilized. While he p ecision P e lec s he p opo ion o alse
posi i es FP, he ecall R de ines he p opo ion o alse
nega i es FN and can be ma hema ically exp essed as ollows:
P
= TP
(TP + FP) and
R
= TP
(TP + FN) , (1
)
wi h TP being he numbe o ue posi i e p edic ions. In o de
o classi y whe he a p edic ion is a TP o FP, he in e sec ion
o e union (IoU) h eshold is used as i measu es he o e lap
be ween he g ound u h and he p edic ed bounding box o
segmen a ion mask, espec i ely. Typical alues o IoU
h esholds a e 0.5 o 0.75. The e y common app oach o
summa ize p ecision and ecall in o one alue is he a e age
p ecision (AP). The AP o a single class is he a e aged
p ecision ac oss all ecall le els. Complemen a ily, he
a e age ecall sco e (AR) a e ages ecall alues o e all IoU
∈ [0.5, 1.0] o each class. The mean a e age p ecision (mAP)
and mean a e age ecall (mAR) ac oss all classes C a e de ined
as (Raphael e al., 2020):
𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚 = 1
𝐶𝐶𝐶𝐶�𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑖𝑖𝑖𝑖
𝐶𝐶𝐶𝐶
𝑖𝑖𝑖𝑖=1
and
mAR
= 1
𝐶𝐶𝐶𝐶�𝑚𝑚𝑚𝑚𝐴𝐴𝐴𝐴𝑖𝑖𝑖𝑖
𝐶𝐶𝐶𝐶
𝑖𝑖𝑖𝑖=1
(2)
As he e alua ion me ics used o his esea ch is based on
COCO (Lin e al., 2014), i should be no ed ha mAP and mAR
sco es a e u he deno ed as AP and AR o simplici y
easons. They a e compu ed o e single (0.5) IoU o he
a e age o hen IoU le els s a ing om 0.5 o 0.95 in s eps o
0.05 ( he la e is u he deno ed as AP @.50:.95). AP and AR
a e also calcula ed o di e en objec scales (small: < 72²
pixels, medium: > 72² & < 214² pixels, la ge: > 214² pixels)
and o di e en maximum numbe o de ec ions pe image (1,
10, 100). Objec scales de ia e om COCO and a e adjus ed
o i scales in he p oposed images.
Addi ional adop ed pe o mance me ics a e he accu acy o
assess he o al numbe o p edic ions ha a e co ec and he
F1 measu e which e enly weighs be ween p ecision and ecall
(Manning e al., 2009):
accu acy
= TP + TN
(TP + TN + FP + FN) 𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎 𝐹𝐹𝐹𝐹1= 2𝑚𝑚𝑚𝑚𝐴𝐴𝐴𝐴
𝑚𝑚𝑚𝑚+𝐴𝐴𝐴𝐴 (3)
4. EXPERIMENTAL RESULTS
This sec ion p esen s he pe o mance e alua ion o di e en
in es iga ed me hods o de ec , segmen and coun ben hic
mega auna ac oss a ying models and da ase s as lis ed in
Table 1. All es uns a e pe o med on he 200 o iginal image
es se wi h no da a augmen a ion.
Table 1. Abb e ia ions o aining s a egies
CM-X-101
Cen e Mask and ResNeX -101
CM-V-99
Cen e Mask and VoVNe V2-99
CM-L-M
Cen e Mask-Li e and MobileNe V2
M-X-101
Mask R-CNN and ResNeX -101
R-X-101
Re inaNe and ResNeX -101
Mona Lü jens e al. / IFAC Pape sOnLine 54-16 (2021) 76–82 79
3.3 Deep Lea ning A chi ec u e and T aining
The deep lea ning a chi ec u e chosen o his s udy is he
ancho - ee one s age ins ance segmen a ion and objec
de ec o Cen e Mask (Lee and Pa k, 2019) in combina ion
wi h he backbone ne wo k ResNeX -101 (Xie e al., 2017).
While objec de ec ion is he cen al ask o abundance and
assemblage s udies, p edic ing masks will be impo an o
u u e size es ima ions and biomass p edic ions o ben hic
species. An ins ance segmen a ion model was he e o e chosen
in o de o adap o di e se esea ch ques ions o u u e
analyses. Also, he model should ope a e on high in e ence
speed while main aining s ong pe o mances o di ec ly
e alua e da ase s on boa d o esea ch essels o s a egic da a
collec ion. Since bo h selec ed a chi ec u es mee he
men ioned equi emen s and u he p oduce excellen esul s
in ecen benchma k challenges such as COCO (Lin e al.,
2014), hey a e an app op ia e choice o he espec i e
compu e ision ask o ma ine imaging.
The backbone ResNeX -101 used o ea u e ex ac ion is an
ad ancemen o he deep esidual ne wo k ResNe (He e al.,
2016) ha has been ecen ly p oposed. Based on ResNe ,
ResNeX -101 ollows he s a egy o epea ing laye s bu
s acks hem pa allel a he han sequen ially. Thus, esul ing in
accu acy imp o emen s while educing he ne wo k
complexi y and numbe o pa ame e s. Fo his s udy he 101
laye ed ne wo k was used.
As a one s age de ec o , Cen e Mask does no ha e a p oposal
s ep and p io i izes in e ence speed. Addi ionally, as an
ancho - ee de ec o , i does no use p ede ined bounding
boxes o iden i y objec s and is hus insensi i e o di e en
da ase s and hype -pa ame e s (e.g. inpu size, scales, e c.).
Hence, ancho - ee de ec o s alle ia e limi a ions o objec s
ha ha e la ge shape a ia ions and a e a he small (Tian e
al., 2019) which is ideal o he selec ed classes used in his
esea ch. Cen e Mask adop s FCOS (Tian e al., 2019) as
de ec ion head ha di ec ly compu es a 4D ec o and a class
label a each p oposed loca ion o di e en le els o ea u e
maps. Then, he spa ial a en ion-guided mask (SAG-Mask)
compu es he segmen a ion masks on each p edic ed box
egion using he spa ial a en ion module (SAM) ha helps he
mask o ocus on signi ican pixels (Lee and Pa k, 2019).
T aining was execu ed on a 64-bi Linux machine equipped
wi h an In el® Xeon® Gold 6254 CPU @ 3.10 GHz and 5
NVIDIA® Tesla® V100 GPU. The base lea ning a e was se o
0.002 and educed by a ac o o 10 a e 25400 and again a e
38100 i e a ions. To educe ea ly o e i ing on highly
di e en ia ed da ase s, he lea ning a e was also educed o
he i s 5080 i e a ions. Addi ionally, a weigh decay was
implemen ed. The maximum numbe o i e a ions was 50800
which co esponds o 20 epochs. All backbone models a e
ini ialized by ImageNe p e- ained weigh s.
3.4 E alua ion P o ocol
To assess he pe o mance o he model, he e alua ion me ics
a e age p ecision, a e age ecall, F1 measu e and accu acy a e
u ilized. While he p ecision P e lec s he p opo ion o alse
posi i es FP, he ecall R de ines he p opo ion o alse
nega i es FN and can be ma hema ically exp essed as ollows:
P
= TP
(TP + FP) and
R
= TP
(TP + FN) , (1
)
wi h TP being he numbe o ue posi i e p edic ions. In o de
o classi y whe he a p edic ion is a TP o FP, he in e sec ion
o e union (IoU) h eshold is used as i measu es he o e lap
be ween he g ound u h and he p edic ed bounding box o
segmen a ion mask, espec i ely. Typical alues o IoU
h esholds a e 0.5 o 0.75. The e y common app oach o
summa ize p ecision and ecall in o one alue is he a e age
p ecision (AP). The AP o a single class is he a e aged
p ecision ac oss all ecall le els. Complemen a ily, he
a e age ecall sco e (AR) a e ages ecall alues o e all IoU
∈ [0.5, 1.0] o each class. The mean a e age p ecision (mAP)
and mean a e age ecall (mAR) ac oss all classes C a e de ined
as (Raphael e al., 2020):
𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚 = 1
𝐶𝐶𝐶𝐶�𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑚𝑖𝑖𝑖𝑖
𝐶𝐶𝐶𝐶
𝑖𝑖𝑖𝑖=1
and
mAR
= 1
𝐶𝐶𝐶𝐶�𝑚𝑚𝑚𝑚𝐴𝐴𝐴𝐴𝑖𝑖𝑖𝑖
𝐶𝐶𝐶𝐶
𝑖𝑖𝑖𝑖=1
(2)
As he e alua ion me ics used o his esea ch is based on
COCO (Lin e al., 2014), i should be no ed ha mAP and mAR
sco es a e u he deno ed as AP and AR o simplici y
easons. They a e compu ed o e single (0.5) IoU o he
a e age o hen IoU le els s a ing om 0.5 o 0.95 in s eps o
0.05 ( he la e is u he deno ed as AP @.50:.95). AP and AR
a e also calcula ed o di e en objec scales (small: < 72²
pixels, medium: > 72² & < 214² pixels, la ge: > 214² pixels)
and o di e en maximum numbe o de ec ions pe image (1,
10, 100). Objec scales de ia e om COCO and a e adjus ed
o i scales in he p oposed images.
Addi ional adop ed pe o mance me ics a e he accu acy o
assess he o al numbe o p edic ions ha a e co ec and he
F1 measu e which e enly weighs be ween p ecision and ecall
(Manning e al., 2009):
accu acy
= TP + TN
(TP + TN + FP + FN) 𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎𝑎 𝐹𝐹𝐹𝐹1= 2𝑚𝑚𝑚𝑚𝐴𝐴𝐴𝐴
𝑚𝑚𝑚𝑚+𝐴𝐴𝐴𝐴 (3)
4. EXPERIMENTAL RESULTS
This sec ion p esen s he pe o mance e alua ion o di e en
in es iga ed me hods o de ec , segmen and coun ben hic
mega auna ac oss a ying models and da ase s as lis ed in
Table 1. All es uns a e pe o med on he 200 o iginal image
es se wi h no da a augmen a ion.
Table 1. Abb e ia ions o aining s a egies
CM-X-101
Cen e Mask and ResNeX -101
CM-V-99
Cen e Mask and VoVNe V2-99
CM-L-M
Cen e Mask-Li e and MobileNe V2
M-X-101
Mask R-CNN and ResNeX -101
R-X-101
Re inaNe and ResNeX -101
Baseline
O iginal da ase
Syn h
Syn he ic image composi ion wi h equal class
dis ibu ion & Baseline
Syn h-Blncd
Syn he ic image composi ion including ex a
glass sponges and so co als
& Baseline
(balanced aining da a se )
T ad. Augm.
T adi ional augmen a ion using b igh ness
(high
, low), colou one (g een, blue), con as
(
high, low), sa u a ion (high, low), sha pness,
blu & Baseline
Fusion
Syn h-Blncd wi h 6,000 images and T ad.
Augm.
wi h 7,000 images combined &
Baseline
4.1 E alua ion o de ec ion, segmen a ion and coun ing
Fo u u e ben hic assemblage and abundance analyses, he
p ecision sco e as well as ecall a e bo h equally impo an as
specimen should nei he be misclassi ied no missed. As can
be seen in Table 2, he ne wo k CM-X-101 ained on Syn h-
Blncd shows he highes bounding box esul s o 76.7 % AP
@.50 and 59 % AR @10 compa ed o all o he me hods.
De ec ions a e accu a e on a ious backg ounds, illumina ion,
came a dis ances and dis o ions (Figu e 2) con i ming ha
au oma ic de ec ion and classi ica ion me hods a e possible
e en on small highly di e se inpu da ase s. Also, high AP and
AR sco es on segmen a ion masks a e achie ed, yielding 67.7
% @.50 and 49.1 % @10, espec i ely (Table 2). Pe o mance
o bounding boxes a e sligh ly highe han ins ance
segmen a ion masks because e y coa se objec bounda ies a e
d awn on each objec including also many i ele an pixels.
Ins ance segmen a ion assigns only objec ele an pixels o a
label and is he e o e compu a ionally mo e ad anced.
I can be u he no ed, ha he ecall a e o single objec s pe
image is much lowe han he ecall o mul iple objec s pe
image. This accoun s o bo h segmen a ion masks and
bounding boxes. Addi ionally, smalle objec s <72² each
poo e esul s han la ge objec s which migh be caused by he
downsampling in he ResNeX backbone esul ing in ewe
ea u es being ex ac ed. Ano he ac o is he ela i ely la ge
a io be ween pixel size and objec size o small objec s which
migh o en lead o posi ioning e o s when compu ing IoU.
The pe o mance o he model on di e en classes can be
u he in es iga ed wi h a con usion ma ix (Table 3) which
is compu ed using a lowe IoU h eshold o a ou a high ecall
especially o small objec s. A i s , i can be seen ha no
specimen a e w ongly classi ied be ween classes. B i le s a s
ha e he highes p ecision o 99 % and so co als ha e he
highes ecall o 93 %. On he o he hand, glass sponges and
b i le s a s a e o en no de ec ed (high FN) and glass sponges
and so co als a e o en misclassi ied wi h backg ound clu e
(high FP). Using he alues o TP, FP and FN, he accu acy
amoun s o 87 %, 73 % and 58 % o b i le s a s, so co als
and glass sponges, espec i ely, esul ing in a mean accu acy
o 73 %.
In o al, 76 ou o 82 glass sponges we e coun ed, 49 ou o 41
so co als and 597 ou o 673 b i le s a s leading o a
pe cen age a ia ion o -7 %, 20 % and -11 % o each
espec i e class.
4.2 E alua ion o da a augmen a ion s a egies
The de ec ion pe o mance o ma ine o ganisms imp o es in
bo h cases using ei he he da a augmen a ion s a egy o
syn he ic image composi ions o he adi ional image
manipula ion echniques. In ac , wi h he Syn h-Blncd da ase ,
he bounding box AP @.50:.95 esul was he mos imp o ed
wi h an inc ease o 24 % o e he Baseline da ase .
Gene ally, AP and AR on bounding boxes a e sligh ly highe
on syn he ically gene a ed image composi ions han adi ional
augmen a ion me hods (Table 2). The disad an age o he
la e is ha no as many images can be c ea ed wi hou he isk
o o e i ing as objec composi ions a e no changing.
Syn he ic gene a ed images ha e p o en o be a success ul
al e na i e and can be gene a ed in a sho amoun o ime as
well. Howe e , wi h ega ds o ins ance segmen a ion
pe o mance, syn he ic image da ase s show in e io esul s on
AP sco es especially o small and medium sized objec s
(Table 2). The eason o his migh be he inco ec
downsizing o o eg ounds as hey could appea pixela ed in
he p ocess o image c ea ion.
Conside ing he p oblem o class imbalance, syn he ic de i ed
images ha e he po en ial o easily balance ou numbe s o
specimen be ween classes. Table 4 shows ha he de ec ion
pe o mance o glass sponges and so co als a e inc eased by
5-16 % o e he Syn h da ase . The pe o mance o b i le
s a s, howe e , is no imp o ed.
Fig. 2. Example images o de ec ion and segmen a ion esul s o es se wi h CM-X-101/Syn h-Blncd a a ying s a ions.
80 Mona Lü jens e al. / IFAC Pape sOnLine 54-16 (2021) 76–82
4.3 Compa ison wi h o he s a e-o - he-a algo i hms
The selec ed Cen e Mask ia ResNeX -101 a chi ec u e was
u he compa ed o o he s a e-o - he a de ec o s and
backbones such as Re inaNe (Lin e al., 2017), Mask R-CNN
(He e al., 2017), MobileNe V2 (Sandle e al., 2018) o
VoVNe V2-99 (Lee and Pa k, 2019). Resul s a e demons a ed
in Table 2 o bounding boxes and segmen a ion masks. Wi h
ega ds o compe ing de ec o s, bo h Cen e Mask and
Re inaNe a e one s age de ec o s whe eas Mask R-CNN is a
wo s age de ec o ha u ilizes a egion-o -in e es p oposal
s ep which ypically p io i izes de ec ion accu acy o e
in e ence speed. Mo eo e , Re inaNe and Mask R-CNN use
ancho boxes o ea u e de ec ion. F om he esul s in Table 2
i is e iden ha Re inaNe pe o ms nea ly as well as
Cen e Mask whe eas Mask R-CNN shows a much lowe
pe o mance conside ing bounding boxes and segmen a ion
masks. I can be no ed ha one s age ancho - ee de ec o s can
pe o m jus as well on unde wa e image y as o he de ec o
ypes.
Fu he mo e, e alua ions on h ee di e en ly deep backbones
we e conduc ed: ResNeX -101 wi h 114.3 million pa ame e s,
VoVNe -99 wi h 96 million pa ame e s and MobileNe V2
wi h 28.7 million pa ame e s (Lee and Pa k, 2019).
No iceably, MobileNe V2 wi h ewe laye s has conside ably
lowe AP and AR esul s han he o he wo. Meanwhile,
conside ing bounding boxes, VoVNe -99 pe o ms nea ly as
well as ResNeX -101 and wi h ega ds o small objec s, AP
and AR esul s a e e en sligh ly highe . The p oblem o
de ec ing small objec s is known o inc ease o e y deep
backbones as hey need mo e inpu in o ma ion o cope wi h
he massi e amoun o pa ame e s (Nguyen e al., 2020). Small
objec s a e desc ibed by ewe pixels and migh no be di e se
enough o eed he ne wo k wi h su icien in o ma ion
inc easing he changes o o e i ing.
Table 2. Summa y o de ec ion esul s wi h bounding boxes (1s ow) and segmen a ion masks (2nd ow/cu si e)
Table 3. Con usion ma ix o CM-X-101/Syn h-Blncd
Table 4. Summa y o pe o mance esul s pe class
Model/Da a
AP.50:.95
bbox
AP.50
bbox
APsmall
bbox
APmedium
bbox
APla ge
bbox
AR1
bbox
AR10
bbox
AR100
bbox
ARsmall
bbox
ARmedium
bbox
ARla ge
bbox
CM-X-101/
Baseline
41.7
35.2
68.2
63.0
25.3
5.70
29.3
21.2
54.7
50.7
21.6
19.4
51.6
44.4
55.2
45.2
25.4
8.90
45.1
32.7
70.8
57.9
CM-X-101/
Syn h
48.8
37.3
71.0
63.0
27.4
4.90
39.1
24.1
62.8
51.7
24.7
20.6
58.8
47.9
64.2
49.5
27.9
7.90
57.3
41.2
77.1
59.5
CM-X-101/
Syn h
-
Blncd
51.8
40.8
76.7
67.7
27.5
4.60
40.2
27.1
66.1
54.8
25.7
22.1
59.0
49.1
63.9
50.6
27.9
7.50
55.7
41.6
77.9
62.5
CM-X-101/
T ad. Augm
48.8
40.2
75.0
70.4
26.9
7.00
38.6
27.6
58.5
52.2
23.0
20.4
55.3
46.8
58.9
47.9
27.2
10.6
50.1
36.8
72.6
58.6
CM-X-101/
Fusion
51.7
41.5
74.1
69.7
27.1
6.60
42.1
29.0
65.1
53.9
24.9
21.7
57.6
47.0
61.6
48.4
27.5
10.6
52.2
37.1
77.0
59.3
CM-V-99/
Syn h
47.9
36.9
72.0
62.9
27.9
4.80
37.0
23.4
62.8
49.8
23.6
20.1
56.6
46.1
61.9
47.8
28.3
7.60
52.6
38.6
77.1
58.2
CM-L-M/
Syn h
27.3
18.4
48.6
32.6
19.1
0.60
19.0
6.30
40.0
34.6
18.3
15.2
39.1
31.8
43.7
33.4
20.0
2.00
34.4
20.3
59.5
50.5
M-X-101/
Syn h
33.3
25.7
53.2
41.6
13.2
1.70
22.7
11.8
53.0
37.9
20.7
15.2
39.2
31.8
40.0
31.9
13.2
3.80
30.6
20.5
60.7
43.6
R-X-101/
Syn h
47.8 70.7 27.9 37.1 62.2 24.2 56.6 61.9 28.4 53.8 76.7
P edic ed
Glass Sponges
So Co als
B i le S a s
Backg ound
Recall
G ound u h
Glass Sponges
58
0
0
24
70.1
So Co als
0
38
0
3
92.7
B i le S a s
0
0
591
82
87.8
Backg ound
18
11
6
P ecision
76.3
77.6
99.0
Accu acy: 73 %
Glass Sponges
So Co als
B i le S a s
Model/Da a
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
CM-X-101/
Baseline
67.7 67.7 41.4 46.3 70.3 69.5 36.8 45.5 80.2 67.9 46.9 13.9
CM-X-101/
Syn h
67.8 66.2 45.3 46.5 69.8 68.8 48.8 52.6 79.2 65.1 52.4 12.8
CM-X-101/
Syn h-Blncd
71.4 69.2 51.4 54.0 76.8 74.9 51.5 55.9 79.9 64.7 52.6 12.6

Mona Lü jens e al. / IFAC Pape sOnLine 54-16 (2021) 76–82 81
4.3 Compa ison wi h o he s a e-o - he-a algo i hms
The selec ed Cen e Mask ia ResNeX -101 a chi ec u e was
u he compa ed o o he s a e-o - he a de ec o s and
backbones such as Re inaNe (Lin e al., 2017), Mask R-CNN
(He e al., 2017), MobileNe V2 (Sandle e al., 2018) o
VoVNe V2-99 (Lee and Pa k, 2019). Resul s a e demons a ed
in Table 2 o bounding boxes and segmen a ion masks. Wi h
ega ds o compe ing de ec o s, bo h Cen e Mask and
Re inaNe a e one s age de ec o s whe eas Mask R-CNN is a
wo s age de ec o ha u ilizes a egion-o -in e es p oposal
s ep which ypically p io i izes de ec ion accu acy o e
in e ence speed. Mo eo e , Re inaNe and Mask R-CNN use
ancho boxes o ea u e de ec ion. F om he esul s in Table 2
i is e iden ha Re inaNe pe o ms nea ly as well as
Cen e Mask whe eas Mask R-CNN shows a much lowe
pe o mance conside ing bounding boxes and segmen a ion
masks. I can be no ed ha one s age ancho - ee de ec o s can
pe o m jus as well on unde wa e image y as o he de ec o
ypes.
Fu he mo e, e alua ions on h ee di e en ly deep backbones
we e conduc ed: ResNeX -101 wi h 114.3 million pa ame e s,
VoVNe -99 wi h 96 million pa ame e s and MobileNe V2
wi h 28.7 million pa ame e s (Lee and Pa k, 2019).
No iceably, MobileNe V2 wi h ewe laye s has conside ably
lowe AP and AR esul s han he o he wo. Meanwhile,
conside ing bounding boxes, VoVNe -99 pe o ms nea ly as
well as ResNeX -101 and wi h ega ds o small objec s, AP
and AR esul s a e e en sligh ly highe . The p oblem o
de ec ing small objec s is known o inc ease o e y deep
backbones as hey need mo e inpu in o ma ion o cope wi h
he massi e amoun o pa ame e s (Nguyen e al., 2020). Small
objec s a e desc ibed by ewe pixels and migh no be di e se
enough o eed he ne wo k wi h su icien in o ma ion
inc easing he changes o o e i ing.
Table 2. Summa y o de ec ion esul s wi h bounding boxes (1s ow) and segmen a ion masks (2nd ow/cu si e)
Table 3. Con usion ma ix o CM-X-101/Syn h-Blncd
Table 4. Summa y o pe o mance esul s pe class
Model/Da a
AP.50:.95
bbox
AP.50
bbox
APsmall
bbox
APmedium
bbox
APla ge
bbox
AR1
bbox
AR10
bbox
AR100
bbox
ARsmall
bbox
ARmedium
bbox
ARla ge
bbox
CM-X-101/
Baseline
41.7
35.2
68.2
63.0
25.3
5.70
29.3
21.2
54.7
50.7
21.6
19.4
51.6
44.4
55.2
45.2
25.4
8.90
45.1
32.7
70.8
57.9
CM-X-101/
Syn h
48.8
37.3
71.0
63.0
27.4
4.90
39.1
24.1
62.8
51.7
24.7
20.6
58.8
47.9
64.2
49.5
27.9
7.90
57.3
41.2
77.1
59.5
CM-X-101/
Syn h
-
Blncd
51.8
40.8
76.7
67.7
27.5
4.60
40.2
27.1
66.1
54.8
25.7
22.1
59.0
49.1
63.9
50.6
27.9
7.50
55.7
41.6
77.9
62.5
CM-X-101/
T ad. Augm
48.8
40.2
75.0
70.4
26.9
7.00
38.6
27.6
58.5
52.2
23.0
20.4
55.3
46.8
58.9
47.9
27.2
10.6
50.1
36.8
72.6
58.6
CM-X-101/
Fusion
51.7
41.5
74.1
69.7
27.1
6.60
42.1
29.0
65.1
53.9
24.9
21.7
57.6
47.0
61.6
48.4
27.5
10.6
52.2
37.1
77.0
59.3
CM-V-99/
Syn h
47.9
36.9
72.0
62.9
27.9
4.80
37.0
23.4
62.8
49.8
23.6
20.1
56.6
46.1
61.9
47.8
28.3
7.60
52.6
38.6
77.1
58.2
CM-L-M/
Syn h
27.3
18.4
48.6
32.6
19.1
0.60
19.0
6.30
40.0
34.6
18.3
15.2
39.1
31.8
43.7
33.4
20.0
2.00
34.4
20.3
59.5
50.5
M-X-101/
Syn h
33.3
25.7
53.2
41.6
13.2
1.70
22.7
11.8
53.0
37.9
20.7
15.2
39.2
31.8
40.0
31.9
13.2
3.80
30.6
20.5
60.7
43.6
R-X-101/
Syn h
47.8 70.7 27.9 37.1 62.2 24.2 56.6 61.9 28.4 53.8 76.7
P edic ed
Glass Sponges
So Co als
B i le S a s
Backg ound
Recall
G ound u h
Glass Sponges
58
0
0
24
70.1
So Co als
0
38
0
3
92.7
B i le S a s
0
0
591
82
87.8
Backg ound
18
11
6
P ecision
76.3
77.6
99.0
Accu acy: 73 %
Glass Sponges
So Co als
B i le S a s
Model/Da a
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
F1
bbox
F1
mask
AP.50:.95
bbox
AP.50:.95
mask
CM-X-101/
Baseline
67.7 67.7 41.4 46.3 70.3 69.5 36.8 45.5 80.2 67.9 46.9 13.9
CM-X-101/
Syn h
67.8 66.2 45.3 46.5 69.8 68.8 48.8 52.6 79.2 65.1 52.4 12.8
CM-X-101/
Syn h-Blncd
71.4 69.2 51.4 54.0 76.8 74.9 51.5 55.9 79.9 64.7 52.6 12.6
5. CONCLUSION AND FUTURE STEPS
In conclusion, he used da a augmen a ion s a egy o
syn he ically de i ed image composi ions p o ed o be a good
al e na i e o equen ly used augmen a ion echniques.
P oblems such as class imbalance can u he easily be
alle ia ed and boos he pe o mance o unde ep esen ed
classes. De ec ion, segmen a ion and coun ing o ben hic
mega auna is a ask ha can be sol ed wi h good pe o mance
using ew a ian o iginal inpu images using ancho - ee one
s age de ec o s. In compa ison wi h o he models, i is e iden
ha he de ec ion p oblem o small objec s is a challenge ye
o be sol ed. Addi ionally, u u e s eps in ol e he
in oduc ion o mo e ben hic mo pho ypes, imp o ed coun ing
o a e duplica ions o o e lapping images and he alloca ion
o posi ion and wa e dep h o each de ec ed specimen o
u u e assemblage s udies.
6. ACKNOWLEDGEMENTS
We hank he anno a o s o he images: Ga in DMello, Diana
Rubio and Seyed Liales ani. Fu he we hank he cap ain and
c ew o RV Pola s e n as well as he scien i ic pa y o he
c uise PS118 o hei suppo . Special hanks go o Au un
Pu se and Huw G i i hs o hei suppo , he da a collec ion
on boa d and he iden i ica ion o ben hic o ganisms.
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