IGF-1 ENHANCES THE OSTEOGENIC ACTIVITY OF BMP-6 IN VITRO AND IN VIVO, AND
TOGETHER HAVE A STRONGER OSTEOGENIC EFFECT THAN WHEN IGF-1 IS COMBINED
WITH BMP-2.
Gus a o A. Rico-Llanos1,2,3, Jose Bece a1,2,3 and Rick Visse 1,2,3
1 Depa men o Cell Biology, Gene ics and Physiology, Uni e si y o Malaga, IBIMA, Malaga, Spain.
2 Ne wo king Resea ch Cen e on Bioenginee ing, Bioma e ials and Nanomedicine, (CIBER-BBN), Malaga, Spain
3 BIONAND, Andalusian Cen e o Nanomedicine and Bio echnology (Jun a de Andalucia, Uni e si y o Malaga).
Bone mo phogene ic p o ein-2 (BMP-2) is widely used in o hopedic su ge y and bone issue
enginee ing because o i s s ong os eogenic ac i i y. Howe e , BMP-2 ea men s ha e
se e al d awbacks and al e na i es a e being explo ed. Since BMP-6 has been demons a ed o
be mo e os eoinduc i e, i s use, ei he alone o oge he wi h o he cy okines, migh be an
in e es ing op ion.
We ha e compa ed he e ec o BMP-2, BMP-6, o insulin-like g ow h ac o -1 (IGF-1), ei he
alone o in combina ion. MC3T3-E1 cells we e ea ed wi h IGF-1 and/o o BMP-2 o -6 and
he exp ession o os eogenic genes, p oli e a ion and alkaline phospha ase (ALP) ac i i y in
i o we e analyzed. The esul s showed ha IGF-1 g ea ly enhanced he BMP-induced
os eogenic di e en ia ion o hese cells and ha he ALP ac i i y in he cul u es was highe
when he combina ion was made wi h BMP-6 han wi h BMP-2. O he in i o expe imen s
showed ha he os eogenic e ec o hese combina ions can be modula ed con olling he
sequen ial adminis a ion o he g ow h ac o s.
Fu he mo e, we ha e es ed he os eogenic po en ial o hese ea men s in i o by loading
hem on o abso bable collagen sponges which we e implan ed in o an ec opic bone o ma ion
model in a s. These expe imen s e ealed ha only BMP-6 was able o induce bone o ma ion
a he used dose and ha he addi ion o IGF-1 con ibu ed o an inc ease o he
mine aliza ion in he implan s.
Hence, he combina ion o BMP-6 wi h IGF-1 migh be a be e al e na i e han BMP-2 o
o hopedic su ge y and bone issue enginee ing app oaches wi h po en ial applica ion h ough
using con olled deli e y sys ems.