Da a A icle
Da a on li e quali y o hos g ass plan s
wi h and wi hou ungal endophy es
P.E. Gundel
a,
n
, M. Helande
b,c
, L.A. Ga ibaldi
d
,
B.R. Vázquez-de-Aldana
e
, I. Zabalgogeazcoa
e
, K. Saikkonen
c
a
IFEVA - CONICET –Facul y o Ag onomy, Buenos Ai es Uni e si y (UBA), A gen ina
b
Sec ion o Ecology, Depa men o Biology, Uni e si y o Tu ku, 20100 Tu ku, Finland
c
Na u al Resou ces and Biomass P oduc ion Resea ch, Na u al Resou ces Ins i u e Finland (Luke), 20520
Tu ku, Finland
d
G upo de In es igación en Ag oecología (AGRECO), Sede Andina, Uni e sidad Nacional de Río Neg o
(UNRN) and Consejo Nacional de In es igaciones Cien íficas y Técnicas (CONICET), Mi e 630, Río Neg o, CP
8400 San Ca los de Ba iloche, A gen ina
e
Depa men o Abio ic S ess, Ins i u o de Recu sos Na u ales y Ag obiología de Salamanca (IRNASA),
Consejo Supe io de In es igaciones Cien íficas (CSIC), Salamanca, Spain
a icle in o
A icle his o y:
Recei ed 20 Ma ch 2016
Recei ed in e ised o m
7 Ap il 2016
Accep ed 12 Ap il 2016
A ailable online 21 Ap il 2016
abs ac
Ce ain Pooideae species o m pe sis en symbiosis wi h ungal
endophy es o Epichloë genus. Al hough endophy es a e known o
impac he ecology and e olu ion o hos species, hei e ec s on
pa ame e s ela ed wi h quali y o plan biomass has been elusi e.
This a icle p o ides in o ma ion abou pa ame e s ela ed wi h
he quali y o plan li e biomass o wo impo an g ass species
(Schedono us phoenix and Schedono us p a ensis) a ec ed by he
symbiosis wi h ungal endophy es (Epichloë coenophiala and Epi-
chloë uncina a, espec i ely). Fou popula ion o igins o S. phoenix
and one o S. p a ensis we e included. Mine al, biochemical and
s uc u al pa ame e s we e ob ained om h ee samples pe ac-
o s combina ion [species (and popula ion o igin) endophy e].
This da a can be po en ially used in o he s udies which, by means
o ‘da a eanalyzing’o me a-analysis, a emp o find gene al-
iza ions abou endophy e e ec s on hos plan li e biomass. The
p esen da a is associa ed wi h he esea ch a icle “Role o olia
ungal endophy es on li e decomposi ion among species and
popula ion o igins”(Gundel e al., In p epa a ion) [1].
Con en s lis s a ailable a ScienceDi ec
jou nal homepage: www.else ie .com/loca e/dib
Da a in B ie
h p://dx.doi.o g/10.1016/j.dib.2016.04.030
2352-3409/&2016 The Au ho s. Published by Else ie Inc. This is an open access a icle unde he CC BY license
(h p://c ea i ecommons.o g/licenses/by/4.0/).
DOI o o iginal a icle: h p://dx.doi.o g/10.1016/j. uneco.2016.03.001
n
Co esponding au ho .
E-mail add ess: [email p o ec ed] (P.E. Gundel).
Da a in B ie 7 (2016) 1469–1472
&2016 The Au ho s. Published by Else ie Inc. This is an open
access a icle unde he CC BY license
(h p://c ea i ecommons.o g/licenses/by/4.0/).
Specifica ions Table
Subjec a ea Biology
Mo e specific sub-
jec a ea
Plan -mic obe in e ac ion
Type o da a Tables and figu es
How da a was
acqui ed
Mine als: ICP-OES (induc i ely coupled plasma op ical emission spec ome y)
me hod. C and N: d y combus ion (Dumas) me hod by Leco T uMac CN- ana-
lyze , Leco Co po a ion, USA. ADF and ADL: Ankom Au oma ed Fibe Analyze
A2000. Alkaloids: HPLC.
Da a o ma Raw and fil e ed
Expe imen al
ac o s
Plan species and o igin, and symbiosis wi h ugal endophy e
Expe imen al
ea u es
Th ee plan issue samples pe combina ion o expe imen al ac o s we e ana-
lyzed o mine al, biochemical and s uc u al cha ac e iza ion.
Da a sou ce
loca ion
Ruissalo Bo anical Ga den, Uni e si y o Tu ku, Finland
Da a accessibili y Da a a e p esen ed in his a icle.
Value o he da a

The da a p esen de ailed in o ma ion abou e ec s o ungal endophy es on pa ame e s ela ed
wi h li e biomass quali y in wo hos g ass species ( wo cul i a s and h ee wild popula ions).

Mine al, biochemical, and s uc u al cha ac e is ics o biomass quali y de e mine, among o he
ecological p ocesses, li e decomposi ion in na u e.

This de ailed in o ma ion can be eused in u u e wo ks looking o gene al pa e ns o ungal
endophy e e ec s on hos biomass quali y and li e decomposi ion.
1. Da a
Raw da a o mine al, biochemical (alkaloids) and s uc u al cha ac e iza ion o biomass li e
p oduced by wo plan species and popula ions [Schedono us p a ensis: he cul i a ‘Kaspe ’( om
Finland); and Schedono us phoenix: he cul i a Ken ucky-31 ( om U.S.) and h ee wild o igin (Go -
land, Åland and Söde manland)] wi h (Eþ) and wi hou (E) ungal endophy es a e p esen ed in he
included excel file online appendix. The file con ains h ee shee s. The shee ‘Chemis y’con ains he
esul s o all analyzed mine als (Ca, Cu, Fe, K, Mg, Mn, P, S, and Zn) and s uc u al pa ame e s (D y
ma e , Ash, ADF and ADL) in he h ee p ocessed samples pe popula ion [i.e. each combina ion o
species (popula ion) and endophy e]. The nex shee ‘N and C’, p esen s esul s om h ee samples
pe popula ion o pe cen age o ni ogen and ca bon o each popula ion. Finally, he shee ‘Alkaloids’
con ains esul s o alkaloid concen a ion (i.e. pe amine and e go aline) in Eþpopula ion ( wo
analyzed samples pe popula ion) and a con ol analysis o confi m ha Epopula ions we e ee o
alkaloids. In his pape , Figs. S1 and S2 show mean alues o each pa ame e (Fig. S1: K, S, P, Mn, Mg,
Ca, Cu, Fe and Zn; and Fig. S2: D y ma e , ash, ADF and ADL) o each popula ion (Figs. S1 and S2).
Da a o ni ogen, ca bon, and C:N a io a e p esen ed in he associa ed esea ch a icle [1].
P.E. Gundel e al. / Da a in B ie 7 (2016) 1469–14721470
2. Expe imen al design, ma e ials and me hods
2.1. Plan ma e ial and expe imen al design
The plan ma e ial used o analyzing he quali y o biomass as a ec ed by ungal endophy es was
p oduced by plan s g owing in a common ga den a Ruissalo Bo anical Ga den (Uni e si y o Tu ku,
Finland). Besides he comme cial cul i a ‘Ken ucky-31’, seeds o S. phoenix ( all escue) we e col-
lec ed om h ee geog aphic loca ions a ound he Bal ic Sea: Åland, (Finland), Go land and Söde -
manland (Sweden). F om each seed lo (i.e. popula ion), a pa o he collec ed seeds was ea ed wi h
hea ed wa e in o de o kill he ungus and o ob ain endophy e- ee plan s. Thus, endophy e-
symbio ic (Eþ; un ea ed) and non-symbio ic seeds (E; ea ed) we e ob ained o each popula ion.
Ten indi idual plan s o each popula ion and symbio ic s a us we e placed a andom in a g id wi h
1m
2
o each plan in 2005 ( o de ails see: [2,3]). Following a simila design, 10 indi idual plan s o S.
p a ensis (meadow escue; cul i a ‘Kaspe ’), symbio ic (Eþ) and non-symbio ic (E) wi h endo-
phy e, we e plan ed in 2008 ( o de ails see: [4]). Along he successi e yea s, all plan s we e checked
unde ligh mic oscope o endophy e p esence o absence o confi m he nominal symbio ic s a us.
A he end o he g owing season (au umn 2011), he abo eg ound biomass o plan s om each
combina ion o species, popula ion, and symbio ic s a us we e ha es ed. The biomass belonging o
he 10 plan s om each ea men was pooled and mixed. Th ee samples pe ea men con aining
ai -d ied lea es and pseudos ems we e aken o un he analyses o biomass cha ac e iza ion.
2.2. Biomass cha ac e iza ion
Samples we e analyzed in e ms o ca bon con en and mine al composi ion (Ca, Cu, Fe, Mg, Mn, N,
K, P, S and Zn). Mine al composi ion was de e mined by he ICP-OES (induc i ely coupled plasma
op ical emission spec ome y) me hod. To al ca bon and ni ogen we e de e mined wi h an au o-
ma ed d y combus ion me hod (Dumas me hod) by Leco T uMac CN- analyze , Leco Co po a ion, USA.
(De ails a e included in he associa ed esea ch a icle [1]). Acid de e gen fibe [ADF: cellulo-
seþligninþash (mine als and silica)] and acid de e gen lignin (ADL: lignin) we e de e mined by
using he fil e bag echnique, wi h an Ankom Au oma ed Fibe Analyze A2000, based on he ana-
ly ical me hod by Goe ing and Van Soes [5].
Fungal alkaloid concen a ion was de e mined on one sample o biomass pe ea men . E go a-
line and i s isome e go alinine we e quan ified by HPLC ollowing a modifica ion o he me hods
desc ibed by Hill e al. [6] and Yue e al. [7]. Pe amine alkaloid concen a ion was de e mined using
he HPLC me hod desc ibed by Ba ke e al. [8] and Yue e al. [9] (see [1]).
Appendix A. Supplemen a y ma e ial
Supplemen a y da a associa ed wi h his a icle can be ound in he online e sion a h p://dx.doi.
o g/10.1016/j.dib.2016.04.030.
Re e ences
[1] P.E. Gundel, M. Helande , L.A. Ga ibaldi, B.R. Vázquez-de-Aldana, I. Zabalgogeazcoa, K. Saikkonen, Role o olia ungal
endophy es on li e decomposi ion among species and popula ion o igins, Fungal Ecology 21 (2016) 50–56.
[2] P.E. Gundel, L.A. Ga ibaldi, M. Helande , K. Saikkonen, Symbio ic in e ac ions as d i e s o ade-o s in plan s: e ec s o
ungal endophy es on all escue, Fungal Di e s. 60 (2013) 5–14.
[3] P.E. Gundel, M. Helande , C. Casas, C.E. Hamil on, S.H. Fae h, K. Saikkonen, Neo yphodium ungal endophy e in all escue
(Schedono us phoenix): a compa ison o h ee No he n Eu opean wild popula ions and he cul i a Ken ucky-31, Fungal
Di e s. 60 (2013) 15–24.
[4] K. Saikkonen, K. Ruokolainen, O. Hui u, P.E. Gundel, T. Pil i, C.E. Hamil on, M. Helande , Fungal endophy es help p e en
weed in asions, Ag ic. Ecosys . En i on. 165 (2013) 1–5.
[5] H.K. Goe ing, P.J. Van Soes , Fo age fibe analyses (appa a us, eagen s, p ocedu es, and some applica ions), U. S. Ag icu. Res.
Se . (1970).
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[6] N.S. Hill, G.E. Ro inghaus, C.S. Agee, L.M. Schul z, Simplified sample p epa a ion o HPLC analysis o e go aline in all
escue, C op Sci. 33 (1993) 331–333.
[7] Q. Yue, S. Logend a, A. F eeho , M.D. Richa dson, Alkaloids o u - ype fine escue (Fes uca Sp.), in: C.W. Bacon, N.S. Hill
(Eds.), Neo yphodium/G ass In e ac ions, 1997, pp. 285–287.
[8] D.J. Ba ke , E. Da ies, G.A. Lane, G.C.M. La ch, H.M. No , B.A. Tappe , E ec o wa e defici on alkaloid concen a ions in
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na ional Symposium on Ac emonium/G ass In e ac ions, 1993, pp. 67–71.
[9] Q. Yue, J. Jonson-Cicalese, T.J. Gian agna, W.A. Meye , Alkaloid p oduc ion and chinch bug esis ance in endophy e-
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