Cullin-RING ligase BioE3 reveals molecular-glue-induced neosubstrates and rewiring of the endogenous Cereblon ubiquitome

Abstract

R.B. and J.D.S. were funded by MCIN/AEI/https://doi.org/10.13039/501100011033, projects PID2023-147399NB-I00 and PID2020-114178 GB-I00 and CEX2021-001136-S and CEX2021-001202-M Severo Ochoa Excellence Program and additional support provided by the Diputación Foral de Bizkaia, Programa Transferencia Tecnológica 2023, project 6/12/TT/2023 /00001, and the Department of Industry, Tourism, and Trade of the Basque Country Government (Elkartek Research Programs); J.J.-J. was funded by grant CNS2022-135307 funded by the MCIN/AEI/https://doi.org/10.13039/501100011033 and the European Union NextGeneration EU/PRTR; V.M., C.G.-P., M.P.-R. and S.C.-L. were funded by FPI grants funded by MCIN/AEI /https://doi.org/10.13039/501100011033 and FSE, PRE2018-086230, PRE2021-099359, PRE2022-104553 and PREP2023-000294, respectively; L.M.-C. was funded by FPU grant FPU20/05282 (funded by Ministerio de Universidades para la Formación de Profesorado Universitario). O.B.-G., J.D.S. and R.B. acknowledge funding by the grant 765445-EU (UbiCODE Program). U.M. was funded by the Spanish MCIU (PID2020-117333 GB-I00 (FEDER/EU)). Access to HPC facilities were granted through the Red Española de Supercomputación (BCV-2021–3-0006 and BCV-2021–2-0005).