1. Da a collec ion and documen a ion
(Please e e also o he o he documen s o he Da a Managemen Plan: DMP_i Assembling; DMP_iii
Assessmen o pe o mances; DMP_i Quan i a i e esul s)
ii. Equipmen se ing
SAMPLE INJECTOR
Figu e 1.ii.1. Diag am o he elec opneuma ic pa . C4D: capaci i ely coupled con ac less conduc i i y
de ec o . F om Musile e al. Analy ica Chimica Ac a 1350 (2025), 343835.
Figu e 1.ii.2. Elec onic ci cui y. Scheme o he de ice componen s managed wi h he MSP430FR2433
Launchpad. I2C: in e -in eg a ed ci cui , a se ial communica ion bus; DAC: digi al- o-analog con e o ; TTL:
ansis o - ansis o logic, on/o -con ol signals. F om Musile e al. Analy ica Chimica Ac a 1350 (2025),
343835.
Po able de ice: DESIGN
Lis o componen s o be housed in he po able case:
- High ol age powe supply (HVPS). Two HVPS o applying a high ol age wi hin ±30kV
- Pneuma ic sec ion. I includes p essu e egula o s, an ai comp essed cylinde , la ching al es, and an ai
al e.
- Hyd aulic sec ion. I includes ese oi s, ubes, injec ion sys em
- De ec o . I consis s o a con ac less conduc i i y de ec o (C4D)
- Sa e y cage. I consis s o a polyca bona e box and a sa e y mic oswi ch
- Mic ocon olle boa d. I includes he elec onic boa d o con olling he en i e de ice
- Ba e y
Po able de ice: SELECTION OF THE INJECTION SYSTEM
Figu e 1.ii.3. Schema ic ep esen a ion o he mic o luidic pa in eg a ing he six-po al e. The g een
numbe s ep esen he leng h o he used ubes exp essed in millime es, while he ed numbe s he in e nal
diame e (mic ome es).
Equipmen se ing
-Feasibili y s udy: selec ion o he injec ion sys em-
Sample
Ca ions solu ion (ammonium, po assium, calcium, sodium magnesium,
li hium, ba ium)
E ec i e capilla y leng h
26 cm
To al capilla y leng h
40 cm
ID capilla y
25 µm
Injec ion ime
2600 ms 0.4 ba
Vol age
20 kV
Bu e
His idine 15 mM, Ace ic acid 50 mM, 18-c own-6 4 mM
-Feasibili y s udy: selec ion o he injec ion sys em -
Sample
Anions solu ion (gamma-hyd oxybu y ic acid, al a-hyd oxybu y ic acid,
and be a-hyd oxybu y ic acid added o a sample o u ine
E ec i e capilla y leng h
70 cm
To al capilla y leng h
85 cm
ID capilla y
25 µm
Injec ion ime
15200 ms 0.4 ba
Vol age
-20 kV
Bu e
A ginine 20 mM, Maleic Acid 10 mM, CTAB 30 µM, ancomycin 5 mM
(pH 7.35)
