Supplementary material 2 from: Reinhart T, Espinosa Prieto A, Begoc T, Tinguy H, Bick F, Chanez E, Beisel J-N, Hardion L (2025) A comparative analysis of hybridisation capture and PCR-based eDNA metabarcoding for monitoring bryophytes in riparian ecosystems. Metabarcoding and Metagenomics 9: e154548. https://doi.org/10.3897/mbmg.9.154548

Author: Reinhart, Thomas; Espinosa Prieto, Armando; Begoc, Thomas; Tinguy, Hugues; Bick, Francis; Chanez, Etienne; Beisel, Jean-Nicolas; Hardion, Laurent

Publisher: Zenodo

DOI: 10.3897/mbmg.9.154548.suppl2

Source: https://zenodo.org/records/17310925/files/oo_1433319.pdf

Supplemen a y ma e ial o :
A compa a i e analysis o hyb idisa ion cap u e and PCR-based eDNA me aba coding
o moni o ing b yophy es in ipa ian ecosys ems
Figu e S1: Visualisa ions o he DNA logoplo o he designed ITS2 p ime pai B aF_B aR,
showing whe e o add degene a e nucleo ides o main ain uni e sali y among b yophy es (1175
sequences o Ma chan iophy a, 108 Dic aniadae, 108 Pleu oca p and 14 o he B yophy a) o
ascula plan s (6797 sequences) and imp o e PCR esul s. The numbe o misma ches o each
axa o he o wa d (x-axis) and e e se (y-axis) a e shown in pie cha s.
1
Figu e S2. Ba code cha ac e is ics o each ITS2 p ime pai es ed in in silico PCR. The mean
amplicon leng h (bp) is displayed o each p ime pai . Ba code co e age (BC) and ba code
speci ici y (Bs) a e p esen ed as pe cen ages.

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