57
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
EVALUATION OF LACTIC ACID PRODUCTION ACTIVITY OF PROBIOTIC
LACTOBACILLI
Abdulaxado a G.Sh.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17313793
Rele ance: Today, lac ic acid is conside ed one o he mos demanded biop oduc s wo ldwide.
This acid is widely used in he ood indus y, pha maceu icals, cosme ology, and many o he sec o s.
The e o e, selec ing sui able aw ma e ials and de e mining op imal bio echnological condi ions o
inc ease p oduc ion e iciency is one o he u gen scien i ic and p ac ical asks. Whey, a by-p oduc
o he dai y indus y, is ega ded as a p omising aw ma e ial o lac ic acid p oduc ion.
Aim o he s udy. One o he impo an asks is o selec he mos ac i e s ains o lac ic acid
bac e ia o e icien lac ic acid p oduc ion and o de e mine he maximum p oduc ion pe iod o lac ic
acid syn hesized by hem. The ob ained esul s will p o ide a scien i ic and p ac ical basis o
op imizing he lac ic acid p oduc ion p ocess, inc easing economic e iciency, and de eloping
en i onmen ally iendly bio echnologies.
Me hods and app oaches. The amoun o lac ic acid was de e mined by a spec opho ome ic
me hod based on he o ma ion o a colo ed complex wi h e ic chlo ide (FeCl₃). In his me hod,
lac a e ions eac wi h FeCl₃ o o m a yellow-g een e ic lac a e solu ion.
• 2 ml o 0.2% FeCl₃ solu ion was aken, and 50 µl o lac ic acid solu ion was added and mixed.
• The op ical densi y o he esul ing solu ion was measu ed a a wa eleng h o 390 nm (using a
1300-SF spec opho ome e ).
• A FeCl₃ solu ion alone was used as a con ol.
• Calib a ion cu e was cons uc ed using s anda d lac ic acid solu ions (X-axis — op ical
densi y, Y-axis — concen a ion).
• Based on his cu e, he concen a ion o lac ic acid in he cul u e luid was calcula ed in mg/ml
uni s.
Resul s. The amoun o lac ic acid in he es ed s ains P. acidilac ici 1G, P. acidilac ici 2G, L.
plan a um 3G, L. b e is 4G, and L. e men um LF1 was de e mined by spec opho ome y. I was
ound ha he highes lac ic acid yield in hese s ains was obse ed on he 4 h day o cul i a ion. On
day 1, L. e men um LF1 p oduced 15.72 g/L o lac ic acid, which inc eased o 40.91 g/L by day 4.
P. acidilac ici 2G p oduced 9.72 g/L on day 1, and 18.91 g/L on day 4. L. plan a um 3G p oduced
om 1.47 g/L on day 1 o 11.25 g/L on day 4. L. b e is 4G p oduced 1.08 g/L on day 1 and 8.01 g/L
on day 4. P. acidilac ici 1G p oduced 3.02 g/L on day 1 and inc eased o 9.39 g/L by day 4. The s udy
esul s demons a ed ha lac ic acid p oduc ion abili y signi ican ly a ies among s ains and depends
on cul i a ion du a ion. The highes p oduce s ain was L. e men um LF1, which eached 40.91 g/L
o lac ic acid on day 4, while L. plan a um 3G was he lowes p oduce , wi h 11.25 g/L on day 4. P.
acidilac ici 2G and L. e men um LF1 s ains also showed a signi ican inc ease in lac ic acid
p oduc ion.
Conclusions. Acco ding o he esul s ob ained by he spec opho ome ic me hod, lac ic acid
p oduc ion capaci y depends on he gene ic cha ac e is ics o he s ain and cul i a ion condi ions. L.
e men um LF1 s ain p oduced he highes amoun o lac ic acid and is ecommended as he mos
p omising s ain o applica ions in medicine, ag icul u e, and he ood indus y.
