74
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DEVELOPMENT OF A TECHNOLOGY FOR OBTAINING DRY EXTRACT AND
ESSENTIAL OIL FROM WORMWOOD HERB
Sadeno a A.A.1
Dau enbeko K.N.2
JSC Sou h Kazakhs an Medical Academy, Shymken , Republic o Kazakhs an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17318925
Rele ance: Wo mwood (A emisia (spp.) is widesp ead in he lo a o Kazakhs an and has long
been used in olk medicine as a medicinal plan o gas oin es inal diseases, as an an i-in lamma o y,
an imic obial, and es o a i e. Cu en ly, he demand o na u al plan ma e ials in he
pha maceu ical, ood, and cosme ic indus ies is g owing.
Ta ge : De elopmen o a echnology o ob aining d y ex ac and essen ial oil, ich in
biologically ac i e compounds, om he plan wo mwood (A emisia spp.), de e mina ion o hei
quali a i e composi ion and subs an ia ion o he possibili y o hei use in he pha maceu ical, ood
and cosme ic indus ies.
Resea ch me hods.
Me hods o aw ma e ial p epa a ion: Collec ion o wo mwood he b and d ying in he shade a
a empe a u e o 40–45 °C. G inding and ac iona ion (pa icle size 3–8 mm).
Me hods o ob aining d y ex ac : Ex ac ion; Mace a ion o pe cola ion wi h 70% e hyl
alcohol. Fil a ion: Vacuum il e . Concen a ion: Ro a y e apo a o unde acuum (≤50 °C). D ying:
Sp ay o acuum d ye .
Me hods o ob aining essen ial oil: Hyd odis illa ion (Cle enge appa a us) o s eam
dis illa ion. Essen ial oil is isola ed and d ied wi h sodium sul a e.
Physicochemical analysis me hods: O ganolep ic analysis (colo , odo , as e, consis ency).
De e mina ion o mois u e, ash con en , and yield. De e mina ion o d y ex ac solubili y.
Chemical analysis me hods: HPLC – de e mina ion o la onoids and lac ones. GC-MS ( GC -
MS ) – de e mina ion o essen ial oil con en .
Me hods o assessing biological ac i i y: De e mina ion o an ioxidan ac i i y (DPPH
me hod). S udy o an imic obial ac i i y (using an imic obial es s ains, aga di usion me hod).
Resul s: A scien i ically based echnology o ob aining d y ex ac and essen ial oil om he
he b o wo mwood ( A emisia) is p oposed. spp .).
* Physicochemical pa ame e s o d y ex ac (mois u e, ash con en , solubili y) we e de e mined
and s anda dized.
* The chemical composi ion was analyzed and he main biologically ac i e subs ances (
la onoids , sesqui e pene lac ones, essen ial oil componen s) we e cha ac e ized.
* The quali a i e and quan i a i e composi ion o he essen ial oil was de e mined using gas
ch oma og aphy-mass spec ome y.
* The yield o he ob ained p oduc s is calcula ed.
* The an ioxidan and an imic obial ac i i y o he ex ac and essen ial oil o wo mwood has
been expe imen ally p o en.
* The p oduc ion o na u al p oduc s sui able o use in he pha maceu ical, ood and cosme ic
indus ies has been subs an ia ed.
The use o local plan ma e ials p omo es impo subs i u ion and he de elopmen o domes ic
p oduc ion.
75
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions: D y ex ac and essen ial oil o wo mwood a e biologically ac i e na u al
p oduc s. The de eloped echnology allows o he e ec i e use o local plan ma e ials, impo
subs i u ion, and he c ea ion o new he bal emedies.
