RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 55
STUDYING THE MECHANISM OF PHYSIOLOGICAL INDICATOR
IMPROVEMENT IN RUMINANTS THROUGH MICROBIOLOGICAL
PROCESSING METHODS
Shi inboye a D.K. 1 dil abookomilo [email protected]
Ku liye a G.Dj. 2 [email protected]
Sunna o a M.Sh. 1 sunnna o [email protected]
1Sama kand S a e Uni e si y o Ve e ina y Medicine, Animal Husband y and
Bio echnology
2Ins i u e o Mic obiology, Academy o Sciences o he Republic o Uzbekis an
h ps://doi.o g/10.5281/zenodo.17322006
Anno a ion
Mode n bio echnological app oaches a e inc easingly equi ed o enhance
biological e iciency and p oduc i i y in la ge and small uminan s in li es ock
a ming. The diges i e sys em o uminan s is closely associa ed wi h mic obial
ac i i y, and hei physiological condi ion can be op imized h ough mic obiological
p ocessing. This a icle in es iga es he e ec i eness o a biop epa a ion de eloped
om an associa ion o celluloly ic bac e ia capable o deg ading cellulose and p obio ic
bac e ia ha es o e he mic obiological balance o he o ganism. The physiological
e ec s o he biop epa a ion on animals, i s ole in imp o ing diges ion, and i s impac
on he immune sys em we e s udied. Expe imen al esul s demons a ed he high
biological ac i i y and en i onmen al sa e y o his biop epa a ion.
Keywo ds. biop epa a ion, celluloly ic bac e ia, p obio ics, symbio ic e ec ,
animal o ganism, e iciency, mic o lo a.
Аннотация
Современные биотехнологические подходы всё чаще востребованы для
повышения биологической эффективности и продуктивности крупного и
мелкого рогатого скота в животноводстве. Пищеварительная система жвачных
тесно связана с микробной активностью, и её физиологическое состояние может
быть оптимизировано посредством микробиологической обработки. В данной
статье исследуется эффективность биопрепарата, созданного на основе
ассоциации целлюлозолитических бактерий, способных расщеплять целлюлозу,
и пробиотических бактерий, восстанавливающих микробиологический баланс
организма. Изучены физиологические эффекты биопрепарата на животных, его
роль в улучшении пищеварения и влияние на иммунную систему.
Экспериментальные результаты продемонстрировали высокую биологическую
активность и экологическую безопасность данного биопрепарата.
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 56
Ключевые слова: биопрепарат, целлюлозолитические бактерии,
пробиотики, симбиотический эффект, организм животных, эффективность,
микрофлора. Anno a siya
Cho achilikda yi ik a mayda ka sh qay a u chi hay onla ning biologik
sama ado ligi a mahsuldo ligini oshi ish uchun zamona iy bio exnologik
yondashu la obo a ko'p oq alab qilinmoqda. Ka sh qay a u chi hay onla ning
o qa hazm qilish izimi mik obla ning aolligi bilan chamba chas bog'liq bo'lib,
ula ning iziologik hola ini mik obiologik qay a ishlash o qali op imallash i ish
mumkin. Ushbu maqola o ganizmning mik obiologik mu ozana ini iklaydigan
sellyuloza a p obiyo ik bak e iyala ni pa chalashga qodi bo'lgan selüloli ik
bak e iyala asso sia siyasidan ishlab chiqilgan biop epa a ning sama ado ligini
o' ganadi. Biop epa a ning hay onla ga iziologik a’si i, o qa hazm qilishni
yaxshilashdagi ahamiya i, immun izimiga a’si i o‘ ganildi. Taj iba na ijala i ushbu
biop epa a ning yuqo i biologik aolligi a ekologik xa sizligini ko' sa di.
Kali so'zla . biop epa a , selüloli ik bak e iyala , p obiyo ikla , simbio ik a'si ,
hay on o ganizmi, sama ado lik, mik o lo a.
In oduc ion. The e is a g owing demand o en i onmen ally sa e and e ec i e
means o imp o e eed diges ion. In his con ex , biop epa a ions based on p obio ic
and celluloly ic bac e ia a e o g ea impo ance in animal husband y. These
p epa a ions no only enhance diges ion bu also help es o e gu mic obio a balance,
s eng hen immuni y, and imp o e p oduc i i y.
The diges i e sys em o uminan s is complex, wi h mic oo ganisms playing a
c ucial ole in diges ion. Celluloly ic bac e ia, in pa icula , b eak down plan cell
walls, con e ing hem in o nu ien s bene icial o he animal. P obio ics help s abilize
gu mic obio a and suppo immune unc ion. The e o e, in es iga ing he e ec s o
biop epa a ions based on bo h ypes o bac e ia on animal physiology is highly
ele an .
In ou coun y, signi ican a en ion is being gi en o he apid ad ancemen o
li es ock a ming and i s ans o ma ion in o a p o i able sec o . This de elopmen is
aimed a imp o ing he popula ion's li ing s anda ds and ensu ing a s able supply o
essen ial ood p oduc s, such as mea and milk, in he domes ic ma ke . In his con ex ,
he ields o e e ina y medicine and mic obiology a e asked wi h de eloping and
implemen ing supplemen a y unc ional biop epa a ions in high-quali y li es ock eed
a ions. These e o s seek o analyze physiological, immunological, and biochemical
changes in animal o ganisms, enhance he p oduc ion o high-quali y animal p oduc s,
and educe p oduc ion cos s.[4,8,11]
Celluloly ic bac e ia a e mic oo ganisms capable o b eaking down cellulose.
They decompose cellulose in o simple o ms, con e ing i in o ca bohyd a es and
o he o ganic compounds. These bac e ia play a c ucial ole in he p ocess o biological
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 57
decomposi ion. Types o Celluloly ic Bac e ia.Celluloly ic bac e ia a e classi ied
in o wo main ypes: ae obic (oxygen- equi ing) and anae obic ( h i ing in oxygen- ee
en i onmen s).Ae obic bac e ia: Cellulomonas, Bacillus, Pseudomonas Anae obic
bac e ia: Clos idium he mocellum, Ruminococcus [7,19]
Cu en ly, he use o en i onmen ally iendly, biologically ac i e addi i es in
li es ock a ming is o g ea impo ance. Celluloly ic bac e ia play a key ole in
imp o ing diges ion due o hei abili y o deg ade cellulose. P obio ic bac e ia a e
essen ial o main aining mic obial balance in he body and enhancing immune
unc ion. Biop epa a ions de eloped om associa ions o hese wo bac e ial ypes
con ibu e o imp o ing animal heal h and p oduc i i y.[6,10,16]
Ma e ials and Me hods. Resea ch Conduc ed a he Labo a o y o P obio ic
Mic obiology and Bio echnology, Ins i u e o Mic obiology, Academy o Sciences o
Uzbekis an.S udies ca ied ou a he Labo a o y o P obio ic Mic obiology and
Bio echnology o he Ins i u e o Mic obiology o he Academy o Sciences o
Uzbekis an ha e ocused on isola ing and analyzing celluloly ic bac e ia. A o al o 27
pu e bac e ial isola es we e ob ained om he s omach luids o small uminan s
(goa s), abbi s, and chickens, as well as om cellulose- eeding e mi es and ba k
bee les. The celluloly ic ac i i y o hese isola es was ex ensi ely s udied. The
mo phological cha ac e is ics o he bac e ial isola es we e examined and iden i ied
using ma ix-assis ed lase deso p ion/ioniza ion ime-o - ligh mass spec ome y
(MALDI-TOF). A p elimina y sc eening o he celluloly ic ac i i y o bac e ial s ains
isola ed om animals was conduc ed, e ealing signi ican celluloly ic ac i i y in
Bacillus species. Among he iden i ied s ains, he highes celluloly ic ac i i y was
obse ed in:4 s ains o Bacillus sub ilis sp. isola ed om goa s omach luid (0.233
U/ml).1 s ain o Bacillus sp. isola ed om e mi es (0.193 U/ml).
Addi ionally, wo selec ed s ains (1-Bacillus sp. and 2-Bacillus sp.) we e
capable o p oducing up o 0.34 mg/ml o p o ein. The highes ac i i y le el (0.38
mg/ml) was de ec ed in 3-B. sub ilis sp. isola ed om goa s omach luid. [2,6,15,16]
Iden i ica ion and Applica ion o Celluloly ic Bac e ial S ains om he
Diges i e Sys em o Animals.The iden i ica ion o celluloly ic bac e ial s ains isola ed
om he diges i e sys ems o animals and hei applica ion in li es ock a ming o
imp o e he diges ibili y o oughage is a highly ele an esea ch a ea. The use o
biop epa a ions based on associa ions o celluloly ic and p obio ic bac e ia de i ed
om a ious domes ic animals has demons a ed signi ican bene i s.A e iew o
scien i ic a icles published by esea che s a he Labo a o y o P obio ic Mic obiology
and Bio echnology, Ins i u e o Mic obiology, Academy o Sciences o Uzbekis an,
e eals he ollowing indings: Bacillus species wi h celluloly ic p ope ies we e
isola ed om he s omach luids o a ious uminan s.The cul u al and mo phological
cha ac e is ics o hese bac e ia we e s udied, and he syn hesis o endoglucanase and
cellobiase enzymes on di e en cellulose-based subs a es was con i med.Gene ic
iden i ica ion o he bac e ial s ains was conduc ed using he 16S RNA me hod,
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 58
con i ming ha he isola es belong o ou s ains o Bacillus sub ilis.The an ibio ic
sensi i i y o hese s ains and hei g ow h a di e en empe a u es we e
analyzed.P elimina y s udies examined he p obio ic p ope ies o bac e ial s ains
isola ed om abbi s, goa s, and e mi es, con i ming hei abili y o su i e in gas ic
and alkaline en i onmen s. Th ee s ains exhibi ed an imic obial ac i i y, o ming
inhibi ion zones o 15–22 mm agains S aphylococcus au eus, Candida albicans, and
Esche ichia coli.The oxici y and sa e y o he bac e ial s ains we e assessed a a S a e
Ve e ina y Commi ee biolabo a o y, whe e hey we e ce i ied as sa e.To enhance he
celluloly ic ac i i y o he bac e ial s ains, esea che s op imized g ow h media and
cul i a ion condi ions:MPB (Mine al Pep one B o h), Hu chinson medium, and whey-
based medium we e es ed, wi h he whey-based medium p o ing he mos
e ec i e.Op imal condi ions o bac e ial g ow h we e de e mined o be pH 5.5 and a
empe a u e o 37°C.The highes enzyme ac i i y (1.56 U/ml) was obse ed in samples
supplemen ed wi h yeas ex ac and b an.
In he inal esea ch phase, he selec ed celluloly ic bac e ial complex was
lyophilized and es ed in abbi physiology expe imen s a he Depa men o Animal
Physiology, Sama kand S a e Uni e si y. The biomass o mula ion was ound o ha e
a posi i e impac on abbi espi a ion, blood pa ame e s, and weigh gain,
demons a ing i s po en ial as a unc ional supplemen in animal nu i ion. [2,4,15]
S ains o Bacillus sub ilis (wi h celluloly ic ac i i y) and Lac obacillus
acidophilus (wi h p obio ic p ope ies) we e selec ed o he s udy. The biop epa a ion
was p epa ed as an associa ion and es ed on Ka akul sheep. The e alua ion included
clinical and biochemical pa ame e s, weigh gain, eed con e sion a io, in es inal
mic o lo a composi ion, and gene al heal h s a us.
Resul s .In he expe imen al g oup ha ecei ed he biop epa a ion:weigh gain
was 12–15% highe compa ed o he con ol g oup;diges ibili y coe icien inc eased
by 8–10%;posi i e changes we e obse ed in leukocy e and hemoglobin le els; he
numbe o lac o- and bi idobac e ia in he in es inal mic o lo a inc eased, while
pa hogenic mic o lo a le els dec eased.The di e ences be ween he expe imen al and
con ol g oups a e p esen ed in he able below: Indica o s Con ol G oup
Expe imen al G oup
Weigh gain (%) 100.0 115.0
Diges ibili y (%) 100.0 110.0
Leukocy e coun (10^9/l) 7.2 8.5
Hemoglobin (g/l) 110.0 125.0
Inc ease in bene icial mic o lo a (%) 100.0 135.0
Reduc ion in pa hogenic mic o lo a (%) 100.0 70.0
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 59
Conclusion. The biop epa a ion de eloped based on he associa ion o
celluloly ic and p obio ic bac e ia posi i ely a ec s he diges i e and immune sys ems
as well as he o e all physiological s a e o animals. I can be e ec i ely used in
li es ock a ming o imp o e nu ien abso p ion and inc ease p oduc i i y.
Biop epa a ions de eloped h ough he combined applica ion o p obio ics and
celluloly ic bac e ia exhibi mul i ace ed e ec s, imp o ing he diges i e and immune
unc ions o a m animals and enhancing hei o e all p oduc i i y. Mo eo e , hey
o e a cos -e ec i e al e na i e o expensi e impo ed p oduc s.
P ac ical Recommenda ions. Biop epa a ions should be applied conside ing
he age and b eed cha ac e is ics. I is ecommended o adjus he dosage based on
mic obiological moni o ing. Re e ences
1- A.S. Damino , F.D. Ib agimo a, De e mina ion o he op imal dose o
e ulene agains chicken eime iosis, Magazine, T ends in he de elopmen o
e e ina y pa asi ology in he CIS and o he coun ies a he beginning o he 21s
cen u y, 202-204 (2021)
2- A. Bach,1,2 A. López-Ga cía,3 O. González-Recio,3,4 G. Elcoso,5 F.
Fàb egas,2 F. Chauchey as-Du and,6,7 and M. Cas ex7 “Changes in he umen and
colon mic obio a and e ec s o li e yeas die a y supplemen a ion du ing he ansi ion
om he d y pe iod o lac a ion o dai y cows”// J. Dai y Sci. 102:6180–6198
h ps://doi.o g/10.3168/jds.2018-16105
3- Abe, K., A. Ueki, Y. Oh aki, N. Kaku, K. Wa anabe, and K. Ueki. 2012.
Anae ocella delica a gen. no ., sp. no ., a s ic ly anae obic bac e ium in he phylum
Bac e oide es isola ed om a me hanogenic eac o o ca le a ms. J. Gen. Appl.
Mic obiol. 58:405–412.
4- Ague e, M. J., M. A. Wa iaux, J. M. Powell, G. A. B ode ick, and C. A nd .
2011. E ec o o age- o-concen a e a io in dai y cow die s on emission o me hane,
ca bon dioxide, and ammonia, lac a ion pe o mance, and manu e exc e ion. J. Dai y
Sci. 94:3081– 3093. h ps://doi.o g/10.3168/jds.2010-4011.
5- AOAC. 1999. O icial Me hods o Analysis. 15 h ed. Associa ion o O icial
Analy ical Chemis s, A ling on, VA.
0
10
20
30
Physiological
Indica o s
Biochemical
Pa ame e s
Rumen En i onmen
and Enzyma ic
Ac i i y
G oup 1 (Con ol) G oup 2 (Cell.) G oup 3 (Cell. + P ob.)
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 60
6- A a ashi, K., T. Tanoue, T. Shima, A. Imaoka, T. Kuwaha a, Y. Momose, G.
Cheng, S. Yamasaki, T. Sai o, Y. Ohba, T. Taniguchi, K. Takeda, S. Ho i, I. I. I ano ,
Y. Umesaki, K. I oh, and K. Honda. 2011. Induc ion o colonic egula o y T cells by
indigenous clos idium species. Science 331:337–341.
7- Baye , E. A., Shoham, Y., Lamed, R. (2006). Cellulose-Decomposing
Bac e ia and Thei Enzyme Sys ems. In: Dwo kin, M. (Ed.), The P oka yo es.
Sp inge .
8- Bakho a Tu ae a, Guzal Кu lie a, Khulka Kamolo a, Nigo a Zukh i dino a.
Isola ion and iden i ica ion o bac e ia wi h celluloly ic ac i i y om he mic o lo a o
animal juice //Jou nal o Wildli e and Biodi e si y. -2023. -V. 7(4). -P. 241-264.
h p://www.wildli e-biodi e si y.com/
9- J. Lapo a, F.C. Fe ei a, V. Ouelle , B. Dado-Senn, A.K. Lea n mo e abou
he ole o umen mic oo ganisms and hei impac on he hos ’s pe o mance and
heal h. Jou nal o Dai y Science (h ps://www.sciencedi ec .com/jou nal/jou nal-o -
dai y-science) Volume 103, Issue 8 (h ps://www.sciencedi ec .com/jou nal/jou nal-
o -dai y-science/ ol/103/issue/8), Augus 2020, Pages 7555-7568.
10- Ka imo , A. A., & To‘ aye , S. R. (2020). Mic obiological Enzymes and
Thei Technological Impo ance. Tashken : Fan a Texnologiya.
11- Ka imo , A. A., & Ismoilo , U. A. (2016). Isola ion o Celluloly ic Bac e ia
and Thei Enzyma ic Ac i i y. Uzbekis an Bio echnology Jou nal, 2(1), 45-52.
12- Ku lie a G. J. Tu ae a B.I. Kamolo a H.F. Kuzie B.U. New possibili ies o
applica ion o celluloly ic bac e iya BACILLUS SUBTILIS isola ed om local
e mi es anacan ho e mes u kes anicus // INTERNATIONAL ASIAN CONGRESS
ON CONTEMPORARY SCIENCES VIII. Aksa ay, Tu kiye. -2023. 328. p.
13- Lynd, L. R., Weime , P. J., an Zyl, W. H., P e o ius, I. S. (2002). Mic obial
Cellulose U iliza ion: Fundamen als and Bio echnology. Mic obiology and Molecula
Biology Re iews, 66(3), 506-577.
14- Leschine, S. B. (1995). Cellulose deg ada ion in anae obic en i onmen s.
Annual Re iew o Mic obiology, 49, 399-426.
15- Naza o , Sh. R., & Jo‘ aye , I. A. (2021). Gene ic Analysis o Bac e ia
In ol ed in Biodeg ada ion P ocesses. Biology and Medicine Jou nal, 5(2), 102-109.
16- Mama o , U. K., & Raximo a, D. S. (2023). Ac i i y o Celluloly ic Bac e ia
in Indus ial Was es and Thei Bio echnological Po en ial. Uzbekis an Mic obiology
Jou nal, 4(1), 60-72.
17- Olsson, L., Wallbe g, O. (2018). Cellulose Biocon e sion o Biop oduc s:
P inciples and Applica ions. CRC P ess.
18- Sukuma an, R. K., Singhania, R. R., Pandey, A. (2016). Mic obial Enzymes
in Biocon e sions o Biomass. Sp inge .
19- Sukuma an, R. K., Singhania, R. R., Pandey, A. (2016). Mic obial Enzymes
in Biocon e sions o Biomass. Sp inge .
RESEARCH FOCUS
ISSN: 2181-3833
Pa anda, baliq, asala i a mo‘ynali hay onla kasallikla i
bilan qa shi ku ashishda dolza b muammola a уechimla |
KONFERENSIYA |
Resea ch Focus Na ional Scien i ic Jou nal, Sama kand 2025 | 61
20- Sanchez, C. (2009). Lignocellulosic esidues: Biodeg ada ion and
biocon e sion by ungi. Bio echnology Ad ances, 27(2), 185-194.
21- Saido , D. B., & To‘ aye , O. T. (2019). Ecological and Bio echnological
Impo ance o Cellulose-Deg ading Mic oo ganisms. Bulle in o he Academy o
Sciences o Uzbekis an, 3(4), 88-95.
22- To‘ aye , O., & Islomo , B. (2018). The Role o Mic oo ganisms in
Indus ial Bio echnology. Tashken : Inno a sion Ziyo.
23- Dec ee o he P esiden o he Republic o Uzbekis an, No. PQ №187, da ed
Ma ch 31, 2022, on "Fundamen al Imp o emen o Pe sonnel T aining in Ve e ina y
and Li es ock Sec o s".
24- Xamido , A., & Qosimo , Sh. (2015). Fundamen als o Mic obiology and
Bio echnology. Tashken : Fan Publishing House.
