233
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF WATER-SOLUBLE VITAMINS IN THE ABOVE-GROUND
PART OF THE CREEPING CLOVER
Abduganie a M.F.1
Xalilo a Sh.R.1
U mano a F.F.1
Sayako a G.M.2
1Tashken pha maceu ical ins i u e, Tashken ci y, Republic o Uzbekis an
2As endiya o Kazakh Na ional Medical Uni e si y, Alma y ci y, Republic o Kazakhs an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333391
Rele ance. i is well known ha i amins play a ole in ene gy me abolism, he biosyn hesis o
amino acids and a y acids, pho o ep ession p ocesses, blood coagula ion, calcium abso p ion, and
mo e. Among he wa e -soluble i amins, he B-g oup i amins (B1, B2, B5, B6, B9/ ola e, B12) and
i amin C a e exc e ed in u ine i consumed in excess, as hey a e no s o ed in he body. The e o e,
hei daily in ake is essen ial. These i amins play an impo an ole in s eng hening he immune
sys em, ensu ing p ope ene gy p oduc ion, and p o ec ing he body om a ious diseases.
The aim o he s udy: o de e mine he con en o wa e -soluble i amins in he he b o he
c eeping plan T i olium epens L. (whi e clo e ) using high-pe o mance liquid ch oma og aphy
(HPLC).
Ma e ials and me hods: he ae ial pa s o he c eeping plan T i olium epens L. we e
collec ed in Tashken ci y in 2025 du ing he ull lowe ing phase and used as he objec o s udy. Fo
he de e mina ion o wa e -soluble i amins using high-pe o mance liquid ch oma og aphy (HPLC),
ace a e bu e sys ems and ace oni ile we e used as eluen s (Figu e 1). Ch oma og aphic
condi ions: HPLC sys em: Agilen 1200, equipped wi h an au osample ; Column: Eclipse XDB-
C18, 5 µm, 4.6 × 250 mm; De ec o : Diode A ay De ec o (DAD), de ec ion a 250 nm; Flow a e:
0.8 mL/min; Eluen composi ion (ace a e bu e : ace oni ile): 0–5 min → 96:4; 6–8 min → 90:10;
9–15 min → 80:20; 15–17 min → 96:4; Column o en empe a u e: 25°C.
Figu e 1. Ch oma og am o wa e -soluble i amins in he ae ial pa o T i olium epens L.
Resul s: he quan i a i e con en o wa e -soluble i amins in he ae ial pa o T i olium epens
L., as de e mined by HPLC, is summa ized in Table 1.
Table 1
№
Vi amins
Re en ion ime
Quan i y, mg/g
1.
С (asco bic acid)
5,356
59,3
2.
В3 (niko inic acid)
5,979
2,54
3.
B1 ( hiamine)
9,670
25,6
234
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
The Con en o Wa e -Soluble Vi amins in he Ae ial Pa o T i olium epens L.
Conclusions: HPLC analysis o he ae ial pa o T i olium epens L. iden i ied asco bic acid
( i amin C) as he p edominan wa e -soluble i amin, suppo ing he plan ’s chemical
cha ac e iza ion.
4.
B6 (py idoxine)
11,657
37,2
5.
B9 ( olic acid)
14,046
42,4
6.
B2 ( ibo la in)
15,012
39,4
