251
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF THE AUTHENTICITY OF THE GLABRUS DRY EXTRACT
Ka aye a N.Y.¹
Tu diye a Z.V.¹
Abdullajono a N.G‘.²
¹ Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
²Ins i u e o Bioo ganic Chemis y named a e A.S. Sodiqo , Tashken ci y, Republic o
Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17333630
Rele ance: acco ding o li e a u e da a, abou 250 species o he plan Rhus belonging o he
amily Anaca diaceae a e known and occu on almos e e y con inen . Bo h he wo ld scien i ic
communi y and esea che s in ou coun y a e s udying he composi ion o Rhus species and explo ing
hei p ac ical applica ions. The smoo h sumac (Rhus glab a), a membe o his amily, was used in
olk medicine by A icenna (Abu Ali ibn Sino). P epa a ions made om his plan we e applied in he
ea men o in es inal ulce s, ea pain, acial ne e pa alysis, o enhance he unc ion o he diges i e
o gans, o dia hea, o s op bleeding, o diabe es, and o ea a ious o he ailmen s.
Pu pose o he s udy: he main objec i e o ou esea ch was o de e mine he au hen ici y o
he condi ionally named “Glab us” d y ex ac ob ained om he smoo h sumac (Rhus glab a) plan
using he hin-laye ch oma og aphy (TLC) me hod.
Ma e ials and Me hods: he s udy was ca ied ou in acco dance wi h Sec ion 2.2.27 (TLC
me hod) o he S a e Pha macopoeia o he Republic o Uzbekis an, Vol. I, Pa 1.
Fi s , 100 mg o he wo king s anda d sample was dissol ed in 10 ml o a me hanol–wa e (70:30)
mix u e and il e ed. The il a e was used as he s anda d solu ion. A colo -de eloping eagen was
p epa ed by mixing equal olumes o he ollowing 0.1 % alcoholic solu ions: aluminum chlo ide, 4-
aminoan ipy ine, an imony (III) chlo ide, and diphenylamine. This eagen was used o sp aying he
pla es.
Fo he es solu ion, 1.0 g o he powde ed sample was dissol ed in 10 ml o a me hanol–wa e
(70:30) mix u e and il e ed. Depending on he ac i e componen s o he plan , di e en mobile-phase
composi ions we e selec ed o sepa a e a ious ac i e subs ances.
P ocedu e: he TLC analysis was pe o med in h ee s ages: Applica ion: A small amoun o
he p epa ed solu ion was applied o one edge o a TLC pla e (coa ed wi h a hin laye o silica gel)
using a mic opipe e. A e applica ion, he sol en quickly e apo a ed, lea ing small spo s on he
silica gel. De elopmen : The spo ed pla e was ca e ully placed in a chambe con aining he mobile
phase. The chambe was closed, and he sol en on was allowed o ise e enly. Iden i ica ion:
A e he colo ed spo s sepa a ed, hey we e immedia ely isible. Because many subs ances a e
colo less, he pla e was ea ed wi h he luo escen eagen and examined unde UV ligh .
The e en ion ac o (R ) was calcula ed as: R = dis ance a eled by he subs ance
dis ance a eled by he sol en on R = ac{ ex {dis ance a eled by he subs ance} }{ ex
{dis ance a eled by he sol en on }}R =dis ance a eled by he sol en
on dis ance a eled by he subs ance. The R alue always lies be ween 0 and 1 and emains
cons an only i ch oma og aphic condi ions—such as he sol en sys em, ype and hickness o
adso ben , sample olume, and empe a u e—a e unchanged.
Resul s: he expe imen al esul s showed ha he spo o he es solu ion co esponded o ha
o he s anda d solu ion, wi h an R alue o 0.6.
252
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions: based on he esul s ob ained, he au hen ici y o he Glab us d y ex ac , when
examined by hin-laye ch oma og aphy, mee s he equi emen s o he S a e Pha macopoeia.
