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Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
PHYTOCHEMICAL STUDY OF COUMARINS IN THE AERIAL PARTS OF
PRANGOS FEDTSCHENKOI (REGEL ET SCHMALH.) KOROV
Komilo Kh.M.
Ik amo a M.Sh.
Mukhi dino a M.K.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
h ps://doi.o g/10.5281/zenodo.17333654
Backg ound. Couma in compounds a e de i a i es o cis-o ho-hyd oxycinnamic acids. They
a e known o hei biological ac i i ies, pa icula ly hei an icoagulan e ec s, while u ocouma ins
ac as pho osensi ize s, i.e., hey inc ease skin sensi i i y o UV adia ion (applied in he ea men o
i iligo). F om his pe spec i e, he sea ch o couma in-con aining plan s, isola ion o hei bioac i e
compounds, iden i ica ion o hei chemical s uc u es, and s udy o hei pha macological ac i i y
a e highly ele an .
In he lo a o Uzbekis an, he genus P angos L. is ep esen ed by 8 species. The couma ins o
he s udied species ha e been poo ly in es iga ed. The ae ial pa s o P angos ed schenkoi g ow in
he moun ainous egions o he Tian Shan. Fo he s udy, he plan was collec ed in he moun ainous
a eas o Kashkada ya du ing la e lowe ing (June), d ied in he shade, and c ushed.
Objec i e. F om 1.0 kg o aw ma e ial, ex ac ion was ca ied ou h ee imes wi h 95%
e hanol. The combined ex ac s we e concen a ed unde acuum, dilu ed wi h wa e (1:2), and
ex ac ed h ee imes wi h 100 mL po ions o die hyl e he . The e he ac ions we e combined, d ied
o e anhyd ous Na₂SO₄, and e apo a ed. The aqueous phase, which was no ans e ed o e he , was
ex ac ed h ee imes wi h 100 mL po ions o e hyl ace a e using a sepa a o y unnel. The e hyl
ace a e ac ion was collec ed.
Me hods. The e he ac ion was ch oma og aphed in he sol en sys em n-hexane–benzene–
me hanol (5:1:4) (Sys em A) o pe oleum e he –benzene–e hanol (5:1:4). The e hyl ace a e ac ion
was ch oma og aphed in n-bu anol–ace ic acid–wa e (5:1:4) (Sys em B). Bo h ch oma og ams we e
d ied, examined unde UV ligh , sp ayed wi h 10% e hanolic KOH, d ied a 105 °C, and ea ed wi h
eshly p epa ed diazo eagen . Spo s cha ac e is ic o couma ins we e e ealed, and hei R alues
we e de e mined.
In he e he ac ion, spo s a R 0.30, 0.62, 0.70, and 0.86 we e obse ed. These spo s appea ed
eddish and showed iole -blue luo escence unde UV ligh .
In he e hyl ace a e ac ion, a eddish spo wi h R 0.80 was de ec ed (Sys em C: ace ic acid–
wa e , 6:4).
The R 0.72 spo om he e he ac ion ch oma og am co esponded o couma in, iden i ied
by compa ison wi h he e e ence compound umbelli e one.
The e hyl ace a e ac ion was e apo a ed o d yness, and he esidue was hyd olyzed by adding
10% sul u ic acid and e luxing wi h a condense in a wa e ba h o 5 hou s. A e cooling, he
hyd olysa e was ex ac ed h ee imes wi h 20 mL po ions o die hyl e he .
Pape ch oma og aphy o he e he -soluble po ion e ealed a spo a R 0.72, which ma ched
umbelli e one as he e e ence compound.
The wa e -soluble po ion emaining a e hyd olysis ga e a spo a R 0.77, iden i ied as a
ca bohyd a e. Using pape ch oma og aphy (Sys em C) and sp aying wi h aniline ph hala e eagen ,
i was iden i ied as D-glucose.
254
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
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Resul s and Conclusion. F om he e he and e hyl ace a e ac ions o P angos ed schenkoi,
he aglycone umbelli e one and D-glucose we e iden i ied a e hyd olysis. Based on he R 0.72 spo
co esponding o umbelli e one and he R 0.80 spo co esponding o umbelli e one glycoside, he
compound p esen in he ae ial pa s o he plan was de e mined o be skimmin (umbelli e one-7-O-
β-D-glucoside).
