265
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
APPLICATION OF THE IMMOBILISATION METHOD TO INCREASE THE
STABILITY OF ENZYMES
Tillae a G.U.
Abdu osi o a N.P.
Tillae a U.M.
Rakhmano a Z.A.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed], [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17334104
Rele ance: I should be no ed ha enzymes ha e ecip ocal and opposi e p ocesses, which
dis up s he s abili y o subs ances in combina ion wi h he second componen . The e a e se e al ways
o a oid his: p o ec ing ac i e subs ances om acidic en i onmen s; p olonging he elease o he
ac i e des uc i e subs ance; and c ea ing a mul i-laye ed dosage o m wi h p og ammable elease
p o iles o indi idual dosage selec ion. The combina ion o ibup o en wi h se a iopep idase, an
enzyme wi h an i-in lamma o y and ib inoly ic e ec s, can educe side e ec s. Howe e , he low
s abili y o se a iopep idase in he gas ic en i onmen equi es he de elopmen o inno a i e
app oaches.
In o de o jus i y ou selec ion, we conduc esea ch wi h model d ug mix u es o a ious
combina ions and di e en coa ing (immobilisa ion) me hods o c ea e a s able d ug mix u e wi h
subsequen ans e o he composi ion in o a a ional dosage o m.
Aim o he s udy: To jus i y and conduc p elimina y expe imen al esea ch on a combined
d ug based on se a iopep idase and ibup o en using immobilisa ion echnologies aimed a inc easing
he s abili y and bioa ailabili y o he ac i e subs ances.
Ma e ials and me hods: Th ee deli e y o ms we e used as esea ch objec s: MKTS (calcium
algina e ma ix), MKTS Pax a (wi h s abilise added), and En o sel (pu i ied complex). The s abili y
and kine ics o ac i e subs ance elease we e analysed using high-pe o mance liquid ch oma og aphy
(HPLC) wi h he Fa m HPLC sys em (DDD: 227, 223, 232 nm; FLD: Ex = 230 nm, Em = 460 nm).
The heo e ical pa o he s udy included an analysis o he p ospec s o using chi osan as a ca ie ,
aking in o accoun i s physicochemical and pha maco- echnological p ope ies.
Resul s: To c ea e a s able medicinal model mix u e, s udies we e conduc ed o jus i y he
selec ion o a ious combina ions and di e en coa ing (immobilisa ion) me hods. To de elop a
me hod o quan i ying he ac i e ing edien s – se a iopep idase and ibup o en – in he mix u e
be o e and a e coa ing (encapsula ion) o he enzyme, high-pe o mance liquid ch oma og aphy
(HPLC) wi h a spec opho ome ic de ec o . Acco ding o HPLC da a, i was ound ha MKTS Pax a
(locally p oduced) and En o sel (wi h added s abilise s) p o ide a mo e s able elease o
se a iopep idase and ibup o en compa ed o he basic calcium algina e o m. In pa icula , he e is a
delayed and con olled elease o ac i e subs ances, which indica es he po en ial e ec i eness o
hese deli e y sys ems. Theo e ical analysis con i ms ha chi osan complexes ha e a numbe o
ad an ages, including esis ance o acidic en i onmen s, mucoadhesi eness, and he abili y o
inc ease bioa ailabili y.
Conclusion: P elimina y expe imen al da a combined wi h heo e ical analysis con i m he
p omise o de eloping a combined d ug based on se a iopep idase and ibup o en using inno a i e
deli e y ma ices. The mos op imal o ms appea o be MKTS Pax a wi h he addi ion o s abilise s
266
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
o based on chi osan. The esul s open up oppo uni ies o u he esea ch and de elopmen o an
e ec i e p olonged- elease dosage o m.
