298
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
QUANTITATIVE DETERMINATION OF L-ARGININE HYDROCHLORIDE IN
THE DRUG "ESCILAR" BY THE TITRATION METHOD
Ulugbeko S.S.
Tashpula o a A.D.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17335164
Rele ance: High d ug quali y is one o he mos impo an c i e ia o p oducing a highly
e ec i e and sa e medica ion. In mode n medicine, medica ions con aining amino acids a e widely
used o he diagnosis, ea men , and p e en ion o many diseases. Compa ed o mo e complex
me hods, i a ion o e s a good p ice-pe o mance a io, being a mo e accessible me hod o
quan i a i e analysis. This me hod allows o he de e mina ion o subs ance con en wi h high
accu acy, down o hund ed hs o a millig am. Compa ed o mo e complex me hods, i a ion o e s a
good p ice-pe o mance a io, being a mo e accessible me hod o quan i a i e analysis. The e o e, he
use o i a ion emains ele an and in demand oday.
Pu pose o he s udy: The aim o he s udy is o de elop a me hod o he quan i a i e
de e mina ion o L-a ginine hyd ochlo ide in he combined in usion d ug “Escila ”, he ac i e
ing edien o which is L-a ginine hyd ochlo ide and escin.
Ma e ials and me hods: The d ug "Escila ", an in usion solu ion, whose ac i e subs ance is
L-a ginine hyd ochlo ide and escin, was used as he objec o he s udy.
10.0 ml o he sample is ans e ed o a 100 ml lask and i a ed wi h 0.1 M sil e ni a e
solu ion un il an o ange-yellow colo is achie ed. Po assium ch oma e solu ion is used as an indica o .
The con en o L-a ginine chlo ide in 1 ml o he d ug (X), in millig ams, is calcula ed using
he ollowing o mula:
𝑋 = 𝑉 • 𝐾 • 21,07
10 ,
whe e: V is he olume o i an used o i a ion, in ml;
K -co ec ion ac o o i a ed solu ion;
21.07 is he amoun o sodium chlo ide in millig ams co esponding o 1 ml o 0.1 M sil e
ni a e solu ion.
All used eagen s and i a ed solu ions, indica o s a e desc ibed in he ele an sec ion o he
S a e Pha macopoeia o he Republic o Uzbekis an.
Based on he s udies conduc ed, i was es ablished ha he con en o L-a ginine hyd ochlo ide
(C6H14N4O2*HCl) in 1 ml o he d ug should be om 27 mg o 33 mg.
Resul s: The s udy ound ha he a e age amoun o L-a ginine hyd ochlo ide in 5 ba ches o
he combined in usion p epa a ion was 30 mg/ml and me he es ablished c i e ia om 27 mg/ml o
33 mg/ml.
Conclusions: Based on he conduc ed s udies, i was es ablished ha he de eloped me hod can
be used o he quan i a i e de e mina ion o L-a ginine hyd ochlo ide in a combined in usion
solu ion, as he ob ained esul s a e eliable and alid. The p esence o o he ac i e componen s o
he d ug does no a ec he analysis esul s. The me hod has been inco po a ed in o he company's
d a pha macopoeial monog aph.
