342
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
SYNTHESIS OF SUPERCHARGED CHITOSAN DERIVATIVES
Akhmedo O.R.
Abdu akhmano J.A.
Shomu o o Sh.A.
Tu ae A.S.
Ins i u e o Bioo ganic Chemis y o he Academy o Sciences o Uzbekis an,
Tashken ci y, Republic o Uzbekis an
е-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17337348
Rele ance: Mode n polyme chemis y is ac i ely e ol ing owa d he de elopmen o
unc ionalized biopolyme s wi h ailo ed physicochemical and biomedical p ope ies. Chi osan, a
de i a i e o chi in, is a polyme con aining eac i e amino g oups, which makes i a p omising aw
ma e ial o he design o no el biologically ac i e mac omolecula sys ems. One o he key
equi emen s o chi osan de i a i es is hei solubili y unde neu al o mildly alkaline condi ions.
Howe e , he limi ed solubili y o na i e chi osan signi ican ly es ic s i s p ac ical applica ions.
The e o e, he de elopmen o highly cha ged chi osan de i a i es emains a ele an and p omising
esea ch di ec ion. In his con ex , he in oduc ion o ca ionic g oups in o he chi osan backbone can
enhance no only i s solubili y a neu al o sligh ly alkaline pH alues bu also i s inhe en biological
ac i i y.
Pu pose o he s udy: Syn hesis o Supe cha ged Chi osan De i a i es Exhibi ing Di e se
Physicochemical Cha ac e is ics.
Ma e ials and me hods: 1 g o chi osan hyd ochlo ide (molecula weigh 230.0 kDa, deg ee
o deace yla ion 85%) was dispe sed in 100 mL o e hanol and allowed o swell o 2 hou s. A e
swelling, a low-molecula -weigh eagen was added a a mola a io o chi osan o cyanoguanidine
= 1:1-4. The eac ion mix u e was s i ed o 5-10 hou s a 60-80°C. Upon comple ion, he p ecipi a e
o med was sepa a ed by decan a ion, dissol ed in 1% hyd ochlo ic acid, and ep ecipi a ed wi h
ace one. The esul ing solid was edissol ed in wa e and pu i ied by dialysis o 48 hou s, wi h i e
changes o dialysis wa e . Finally, he pu i ied p oduc was subjec ed o lyophiliza ion and analyzed.
Resul s: Upon eac ion o cyanoguanidine wi h he amino g oups o chi osan, a nucleophilic
addi ion occu s, leading o he o ma ion o guanidinium moie ies. The conduc ed s udies
demons a ed ha by a ying he eac ion condi ions speci ically he mola a io o eac an s, eac ion
ime, and empe a u e i is possible o ob ain chi osan de i a i es wi h a deg ee o subs i u ion anging
om 0.15 o 0.64 mol%. The p esence o guanidinium g oups in he chi osan backbone was
con i med by FTIR and NMR spec oscopy. In oduc ion o guanidinium agmen s in o he
mac omolecula chain signi ican ly imp o ed he solubili y o chi osan ac oss a wide pH ange. While
na i e chi osan is insoluble in neu al and alkaline media, he syn hesized de i a i es emained
soluble wi hin he pH ange o 3 o 9.
In addi ion, he included guanidine g oups inc eased he o e all cha ge o chi osan (38 mV)
and he ζ-po en ial alue was 45-58 mV.This inc ease in posi i e cha ge may ha e a di ec impac on
he physiological p ope ies o chi osan. Fo ins ance, s onge elec os a ic in e ac ions wi h cell
memb anes could enhance bo h i s an imic obial ac i i y and i s cell adhesion capaci y, he eby
b oadening he po en ial biomedical applica ions o guanidinium- unc ionalized chi osan.
Conclusions: Thus, he syn hesized supe cha ged chi osan de i a i es con aining guanidinium
g oups exhibi imp o ed physicochemical cha ac e is ics, including an expanded solubili y ange and
343
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
an inc eased posi i e su ace cha ge. These p ope ies indica e he po en ial o he modi ied chi osan
de i a i es o use in a ious biomedical applica ions.
This s udy ecei ed inancial suppo om he Inno a i e De elopmen Agency o he Republic
o Uzbekis an (P ojec No. FL-8824063355).
