346
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF THE COMPOSITION OF SOLUBLE VITAMINS IN RAW
MATERIALS OF TAGETES PATULA L. USING THE HPLC METHOD
Ak amo a N.Sh.
Ismoilo a G.M.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17337404
Rele ance: In olk medicine, in usions and decoc ions ob ained om he in lo escences o
Tage es pa ula L. a e widely used. The ichness o he plan 's composi ion wi h biological subs ances
gi es i an i-in lamma o y, an ibac e ial, an ioxidan , diu e ic, seda i e, and gas op o ec i e
p ope ies. Soluble i amins we e s udied o a deepe s udy o he chemical composi ion o he aw
ma e ial o he plan Tage es pa ula L. o he in oduc ion o p epa a ions made om i in o medical
p ac ice.
The absence o side e ec s and low cos o p oduc ion o medicinal plan aw ma e ials allows
i o be widely used in he mode n pha maceu ical indus y in he de elopmen o d ugs ha combine
a wide spec um o pha macological ac i i y. Due o he na u al and sa e na u e o his aw ma e ial,
i is a p io i y in he p oduc ion o e ec i e and cos -e ec i e medicines. In he u u e, he sea ch o
new sou ces o medicinal plan s o he de elopmen o sa e and e ec i e medicines is a p essing
issue, and o achie e his goal, i is necessa y o expand na u al aw ma e ial esou ces.
Objec i e o he s udy. De e mina ion o soluble i amins in Tage es pa ula L. aw ma e ials
by HPLC me hod.
Ma e ials and me hods o esea ch. The con en o soluble i amins in Tage es pa ula L. aw
ma e ials was de e mined by he me hod o high-pe o mance liquid ch oma og aphy (HPLC). The
s udy used an Agilen Technologies 1200 ch oma og aph wi h a DAD de ec o , and a 75x4.6 mm
Disco e y HSC column was used in he analysis.
Vi amins we e iden i ied a a wa eleng h o 250 nm using a diode ma ix de ec o . The low
a e was 0.8 ml/min. A mix u e o ace a e bu e and ace oni ile was used as an eluen .
The eluen a io was changed as ollows:
• 0-5 minu es: 96:4
• 6-8 minu es: 90:10
• 9-15 minu es: ( a io no speci ied - please cla i y)
• 15-17 minu es: 96:4
The analysis was ca ied ou unde he mos a ic condi ions a oom empe a u e. The olume
o he in oduced samples is 5 μl. Fi s , wo king s anda d solu ions we e in oduced in o he HPLC
sys em, and hen solu ions o he p epa ed samples.
Expe imen al pa . Fo analysis, 5-10 g o aw ma e ial is weighed on an analy ical balance,
ans e ed o a 300 ml la lask, and 50 ml o 40% e hanol is added o he lask. The mix u e was
boiled o 1 hou unde condi ions o igo ous s i ing, equipped wi h a magne ic s i e and a e e se
coole . The mix u e was hen s i ed a oom empe a u e o 2 hou s.
The mix u e is se led and il e ed. Ano he 25 ml o 40% e hanol was added o he esidue
emaining in he lask, and he ex ac ion p ocess was epea ed wice mo e. The ob ained il a es
we e combined, placed in a 100 ml olume ic lask, and illed o he line o olume wi h 40% e hanol
(5-10%).
347
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
The esul ing solu ion was placed in a cen i uge a a a e o 7000 pm o 10 minu es. The op
laye o he cen i uga e was sepa a ed o analysis.
Wo king solu ions o wa e -soluble i amins wi h a concen a ion o 1 mg/ml we e p epa ed.
Conclusion. Soluble i amins in Tage es pa ula L. aw ma e ials we e de e mined by high-
pe o mance ch oma og aphy. The con en o hiamine (B1), ibo la in (B2), nico inic acid (PP),
py idoxine (B6), olic acid (B9), and asco bic acid was de e mined as ollows - py idoxine - 23.72
mg/g, ibo la in - 15.36 mg/g, and asco bic acid - 11.6 mg/g.
