353
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ANALYSIS OF FLAVONOID COMPOSITION OF ALLIUM MOTOR BY HPLC
Yuldoshe S.S.
Na ional Uni e si y o Uzbekis an, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17337480
Rele ance: In ecen yea s, he isola ion o bioac i e compounds om na u al sou ces and he
e alua ion o hei pha maceu ical po en ial ha e become impo an di ec ions o scien i ic esea ch.
In pa icula , plan s o he genus Allium ha e long been used in adi ional medicine. Thei chemical
composi ion has been shown o include la onoids, phenolic acids, i amins, and o he biologically
ac i e compounds. In his con ex , a comp ehensi e chemical analysis o Allium mo o and he
assessmen o i s pha maceu ical and bioac i e p ope ies ep esen a ele an ask.
Aim o he s udy. The main aim o his s udy was o iden i y la onoids p esen in he ex ac s
o ege a i e pa s o Allium mo o using high-pe o mance liquid ch oma og aphy (HPLC), o
de e mine hei quan i a i e p opo ions, and o subs an ia e hei pha maceu ical signi icance.
Me hods. As esea ch objec s, e hanol ex ac s we e p epa ed om he lea es and bulbs o
Allium mo o . Analy ical es s we e ca ied ou using an Agilen HPLC sys em ope a ing wi h
ChemS a ion so wa e. A diode a ay de ec o (DAD) was used a wa eleng hs o 254 nm, 265 nm,
and 281 nm. S anda d e e ence subs ances such as apigenin, u in, and es e a ol we e applied o
compa ison.
Resul s. HPLC analysis con i med he p esence o se e al la onoid compounds in Allium
mo o ex ac s. Thei ela i e con en s a e p esen ed in Table 1.
Table 1.
Rela i e con en o la onoids in Allium mo o ex ac (based on HPLC esul s)
№
Compound
Re en ion ime (min)
Rela i e con en (%)
1
Apigenin
1.71–1.73
18.3 – 24.0
2
Rabinin
1.93–1.94
4.6 – 40.9
3
Ru in
2.35
3.7 – 3.9
4
Res e a ol
7.13
3.7 – 22.3
5
O he la onoids
1.62–1.81
16.4 – 46.8
The esul s indica e ha Allium mo o is ich in la onoids. Rabinin and apigenin we e
iden i ied as he main componen s, while es e a ol and u in also made up a signi ican p opo ion
o he composi ion. The di e si y o la onoids demons a es he s ong an ioxidan po en ial o he
plan . Fu he mo e, he high con en o abinin sugges s possible immunomodula o y and an i-
in lamma o y p ope ies. The conside able p esence o apigenin suppo s i s seda i e and an ialle gic
ac i i y. The de ec ion o es e a ol indica es po en ial ca diop o ec i e and an icance e ec s.
Compa ed o da a on o he Allium species epo ed in he li e a u e, Allium mo o appea s o be
dis inguished by i s ichness in bioac i e compounds. Thus, ex ac s o his plan may ep esen a
p omising sou ce o he de elopmen o new phy opha maceu icals.
Conclusions. Allium mo o is ich in la onoids, wi h abinin, apigenin, u in, and es e a ol
being he main componen s. The chemical composi ion o he plan con i ms i s an ioxidan and
pha maceu ical po en ial. The indings p o ide a scien i ic basis o he de elopmen o new
phy op epa a ions and hei use in clinical pha macy.
