Biodi e si y Da a Jou nal 12: e118010
doi: 10.3897/BDJ.12.e118010
Da a Pape
The InBIO Ba coding Ini ia i e Da abase: DNA
ba codes o O hop e a om Po ugal
Síl ia Pina , Joana Paupe io , F ancisco Ba os , Cá ia Cha es , Filipa MS Ma ins ,
Joana Pin o , Joana Ve íssimo , Vanessa A Ma a , Ped o Beja , Sónia Fe ei a
‡ CIBIO, Cen o de In es igação em Biodi e sidade e Recu sos Gené icos, InBIO Labo a ó io Associado, Campus de Vai ão,
Uni e sidade do Po o, 4485–661 Vai ão, Vila do Conde, Po ugal
§ BIOPOLIS P og am in Genomics, Biodi e si y and Land Planning, CIBIO, Campus de Vai ão, 4485–661 Vai ão, Vila do
Conde, Po ugal
| CIBIO, Cen o de In es igação em Biodi e sidade e Recu sos Gené icos, InBIO Labo a ó io Associado, Ins i u o Supe io de
Ag onomia, Uni e sidade de Lisboa, Lisboa, Po ugal
¶ Eu opean Molecula Biology Labo a o y, Eu opean Bioin o ma ics Ins i u e, Wellcome Genome Campus, Hinx on,
Camb idge, Uni ed Kingdom
# Rua da Ei a 3, S. Sal ado , 2550-251, Ce cal, Po ugal
¤ EBM, Es ação Biológica de Me ola, P aça Luis de Camoes, Me ola, Me ola, Po ugal
Co esponding au ho : Sónia Fe ei a ([email p o ec ed])
Academic edi o : Edwa d Bake
Recei ed: 30 Dec 2023 | Accep ed: 24 Feb 2024 | Published: 15 May 2024
Ci a ion: Pina S, Paupe io J, Ba os F, Cha es C, Ma ins FM, Pin o J, Ve íssimo J, Ma a V, Beja P, Fe ei a S
(2024) The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om Po ugal. Biodi e si y Da a
Jou nal 12: e118010. h ps://doi.o g/10.3897/BDJ.12.e118010
Abs ac
Backg ound
The InBIO Ba coding Ini ia i e (IBI) O hop e a da ase con ains eco ds o 420 specimens
co e ing all he ele en O hop e a amilies occu ing in Po ugal. Specimens we e collec ed
in con inen al Po ugal om 2005 o 2021 and we e mo phologically iden i ied o species
le el by axonomis s. A o al o 119 species we e iden i ied co esponding o abou 77% o
all he o hop e an species known om con inen al Po ugal.
‡,§,| ‡,¶ # ‡,§ ‡,§
‡,§ ‡,§ ‡,§ ‡,§,| ‡,§,¤
© Pina S e al. This is an open access a icle dis ibu ed unde he e ms o he C ea i e Commons A ibu ion License (CC BY
4.0), which pe mi s un es ic ed use, dis ibu ion, and ep oduc ion in any medium, p o ided he o iginal au ho and sou ce a e
c edi ed.
New in o ma ion
DNA ba codes o 54 axa we e made public o he i s ime a he Ba code o Li e
Da a
Sys em (BOLD). Fu he mo e, he submi ed sequences we e ound o clus e in 129 BINs
(Ba code Index Numbe s), 35 o which we e new addi ions o he Ba code o Li e Da a
Sys em (BOLD). All specimens ha e hei DNA ba codes publicly accessible h ough BOLD
online da abase. S enobo h us linea us is eco ded o he i s ime o con inen al
Po ugal. This da ase g ea ly inc eases he knowledge on he DNA ba codes and
dis ibu ion o O hop e a om Po ugal. All DNA ex ac ions and mos specimens a e
deposi ed in he IBI collec ion a CIBIO, Resea ch Cen e in Biodi e si y and Gene ic
Resou ces.
Keywo ds
O hop e a, species dis ibu ions, con inen al Po ugal, DNA ba code, cy och ome c
oxidase subuni I (COI)
In oduc ion
Insec s a e e y challenging o s udy due o hei as onishing biological di e si y, he lack o
axonomic expe ise o se e al g oups and adi ional mo phology-based species
iden i ica ion being o en logis ically o inancially unsus ainable. DNA me aba coding is
apidly eme ging o o e come hese challenges ( an Klink e al. 2022), bu i s applica ion
elies on he exis ence o comp ehensi e e e ence collec ions o DNA ba codes. This
me hod iden i ies mul iple species om a mixed sample, based on DNA ba coding using
high- h oughpu sequencing (HTS) o a speci ic DNA ma ke , usually he mi ochond ial
cy och ome c oxidase I (COI) gene. DNA ba coding is a molecula biology me hod o
species iden i ica ion ha elies on he compa ison o a sho mi ochond ial DNA sequence
o in e es o a lib a y o sequences wi h known species iden i y (Hebe e al. 2003).
Hence, o hese compa isons, i is impo an o gua an ee ha a good sequence lib a y is
being used, i.e. all DNA ba codes come om specimens iden i ied by axonomis s
(Chimeno e al. 2023). The Ba code o Li e Da a Sys ems (BOLD) is an in e na ional
ba code e e ence da abase made a ailable in 2007 (Ra nasingham and He be 2007)
and la ge na ional ba coding ini ia i es wo ldwide ha e con ibu ed o inc easing his lib a y
(Janzen and Hallwachs 2011,Geige e al. 2016, deWaa d e al. 2019). In Po ugal, he
InBIO Ba coding Ini ia i e (IBI) was he i s p ojec aimed o de elop a e e ence collec ion
o DNA ba coding sequences, ha ing la gely ocused on Po uguese in e eb a e axa,
pa icula ly insec s (Fe ei a e al. 2018).
O hop e a a e a di e se g oup o he bi o ous insec s and playing an impo an ole in
ecosys em unc ioning as bo h p ima y consume s in di e en habi a s and signi ican ood
sou ces o highe ophic le els (e.g. bi ds, mammals) (Ca y e al. 2018, Valdez and
C yan 2013). Due o he s ong connec ion wi h plan s, o hop e ans a e good indica o s o
2Pina S e al
habi a changes and, he e o e, o en used in en i onmen al moni o ing and
assessmen
(Bie inge e al. 2013, Vasconcelos e al. 2019). Howe e , despi e hei unc ional
impo ance, hey a e s ill poo ly s udied in a eas wi h high le els o endemic species, such
as he Ibe ian Peninsula (Hochki ch e al. 2016). S udies on he Po uguese O hop e a
auna ha e been published in a e y sca e ed manne o e ime, gene ally lacking
comp ehensi e in en o ies o ocusing on ce ain O hop e a axa (Pina e al. 2017).
Al hough some o hese mo e ecen s udies epo new indings o he coun y (e.g.
Lemos e al. (2016), Mon ei o e al. (2016)), he p ime in o ma ion, such as which species
occu in Po ugal and hei dis ibu ions, emains e y incomple e.
In Eu ope, la ge DNA ba coding ini ia i es ha e been es ablished mainly in he no he n
and cen al coun ies (Gay án e al. 2020). In he s udy conduc ed by Hawli schek e al.
(2017), DNA ba codes o O hop e a om ou ba coding ini ia i es (Ba coding Fauna
Ba a ica (Ge many), Ge man Ba code o Li e, Aus ian Ba code o Li e and Swiss Ba code
o Li e) comp ising h ee cen al Eu opean coun ies (Aus ia, Ge many and Swi ze land)
we e made a ailable. A o al o 748 COI sequences we e ob ained o se e al cen al
Eu opean O hop e a axa ha also occu in o he coun ies, namely in Po ugal. This s udy
also showed ha ba coding s udies can be success ully applied o O hop e a e ealing an
o e all cong uence o 76.2% o he 127 O hop e a axa in he s udy. Howe e , mo e wo k
is necessa y o c ea e a lib a y o ba codes o he Eu opean O hop e a because he
ep esen a ion o ce ain g oups (e.g. Te igidae, G yllidae and B adypo inae) (Kasalo e al.
2023) and egional a eas, such as he sou he n Eu opean peninsulas, emains insu icien .
The Ibe ian Peninsula is a known ho spo o endemic O hop e a species, bu many o i s
species emain un ep esen ed in he DNA ba coding e e ence collec ions.
The IBI O hop e a da ase con ains eco ds o 420 specimens o O hop e a collec ed in
con inen al Po ugal, all mo phologically iden i ied o species le el, o a o al o 119
species. Ou esul s cons i u e a i s s ep in he cons uc ion o a DNA ba code da abase
o a cu a ed e e ence collec ion o Ibe ian O hop e a species.
Gene al desc ip ion
Pu pose: This da ase aims o p o ide a con ibu ion o he knowledge on DNA ba codes o
Po uguese O hop e a. Such a lib a y should acili a e DNA-based iden i ica ion o species
o bo h adi ional molecula s udies and DNA me aba coding s udies. Fu he mo e, i
cons i u es a aluable esou ce o axonomic esea ch on Ibe ian O hop e a and hei
dis ibu ion.
Addi ional in o ma ion: A o al o 420 specimens o o hop e ans we e collec ed and DNA
ba coded (Table 1, Suppl. ma e ial 2) co esponding o 119 species, abou 77% o all he
o hop e an species known om con inen al Po ugal (Ai es and Menano 1915, Pina e al.
2017, GBIF 2023, IUCN 2023). Figs 1, 2 illus a e examples o he di e si y o species ha
a e pa o he da ase o dis ibu ion da a and DNA ba codes o Po uguese O hop e a.
The da ase includes 39 Ibe ian endemic species, o which six occu only in con inen al
Po ugal: Ephippige ida osae, Lluciapoma esius anapaulae, Neocallic ania ba osi,
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 3
Neocallic ania se a a, P e olepis lusi anica and Pycnogas e cuculla us (Table 1).
Addi ionally, he species S enobo h us linea us is eco ded o he i s ime o Po ugal in
his da ase . A ull-leng h ba code o 658 bp was ob ained o all 420 specimens. This
da ase con ibu es signi ican ly o he ep esen a ion o bo h species and gene ic di e si y
o O hop e a in public lib a ies. O he 120 axa ba coded, DNA ba codes o 54 a e made
public o he i s ime (ma ked wi h # in Taxa ield o Table 1). The submi ed sequences
we e ound o clus e in 129 BINs, 35 o which we e new o BOLD (unique BINs, ma ked
wi h " in Taxa ield o Table 1).
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Ac o ylus insub icus (Scopoli, 1786) INV02543 IBIOR108-17 BOLD:AAP4452 OR974638
Ac ididae Ac o ylus insub icus (Scopoli, 1786) INV02544 IBIOR109-17 BOLD:AAP4452 OR974794
Ac ididae Ac o ylus insub icus (Scopoli, 1786) INV05545 IBIOR196-22 BOLD:AAP4452 OR974607
Ac ididae Ac o ylus insub icus (Scopoli, 1786) INV06642 IBIOR436-22 BOLD:AAP4452 OR974455
Ac ididae Ac o ylus pa uelis (He ich-Schä e ,
1838)
INV00063 IBIOR002-17 BOLD:AAJ6293 OR974610
Ac ididae Ac o ylus pa uelis (He ich-Schä e ,
1838)
INV03740 IBIOR326-22 BOLD:AAJ6293 OR974601
Ac ididae Ac o ylus pa uelis (He ich-Schä e ,
1838)
INV05270 IBIOR392-22 BOLD:AAJ6293 OR974494
Ac ididae Ac o ylus pa uelis (He ich-Schä e ,
1838)
INV10594 IBIOR535-22 BOLD:AAJ6293 OR974432
Ac ididae Aiolopus puissan i De au , 2005 INV00065 IBIOR004-17 BOLD:AAZ1689 OR974633
Ac ididae Aiolopus puissan i De au , 2005 INV02502 IBIOR067-17 BOLD:AAZ1689 OR974542
Ac ididae Aiolopus s epens (La eille, 1804) INV00066 IBIOR005-17 BOLD:AAP4386 OR974796
Ac ididae Aiolopus s epens (La eille, 1804) INV02503 IBIOR068-17 BOLD:AAP4386 OR974414
Ac ididae Aiolopus s epens (La eille, 1804) INV02539 IBIOR104-17 BOLD:AAP4386 OR974707
Ac ididae Aiolopus s epens (La eille, 1804) INV02540 IBIOR105-17 BOLD:AAP4386 OR974749
Ac ididae Aiolopus s epens (La eille, 1804) INV04053 IBIOR351-22 BOLD:AAP4386 OR974447
Ac ididae Aiolopus s epens (La eille, 1804) INV06052 IBIOR406-22 BOLD:AAP4386 OR974623
Ac ididae Anac idium aegyp ium (Linnaeus,
1764)
INV02504 IBIOR069-17 BOLD:AAO1248 OR974491
Table 1.
Lis o axa ha we e collec ed and DNA ba coded wi hin his p ojec . In column Taxa: - indica es
Ibe ian endemic species; - indica es con inen al Po ugal endemic species; - indica es axa
wi hou a public DNA ba code p io o his s udy; " - indica es unique BINs.
1
2 #
4Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Anac idium aegyp ium (Linnaeus,
1764)
INV02505 IBIOR070-17 BOLD:AAO1248 OR974554
Ac ididae Anac idium aegyp ium (Linnaeus,
1764)
INV04117 IBIOR166-19 BOLD:AAO1248 OR974781
Ac ididae A cyp e a o nosi Bolí a , 18841 INV02598 IBIOR188-22 BOLD:AER6128 OR974776
Ac ididae A cyp e a o nosi Bolí a , 18841 INV02599 IBIOR189-22 BOLD:AER6128 OR974669
Ac ididae Calepho us comp essico nis (La eille,
1804)
INV02562 IBIOR120-17 BOLD:AEA6681 OR974480
Ac ididae Calepho us comp essico nis (La eille,
1804)
INV02563 IBIOR121-17 BOLD:AEA6681 OR974777
Ac ididae Callip amus ba ba us (Cos a, 1836) INV00067 IBIOR006-17 BOLD:ACQ0538 OR974431
Ac ididae Callip amus ba ba us (Cos a, 1836) INV00068 IBIOR007-17 BOLD:ACQ0538 OR974606
Ac ididae Callip amus ba ba us (Cos a, 1836) INV02550 IBIOR113-17 BOLD:ACQ0538 OR974538
Ac ididae Callip amus ba ba us (Cos a, 1836) INV03646 IBIOR314-22 BOLD:ACQ0538 OR974580
Ac ididae Callip amus ba ba us (Cos a, 1836) INV04028 IBIOR346-22 BOLD:ACQ0538 OR974686
Ac ididae Callip amus wa enwylianus Pan el,
1896
INV00069 IBIOR008-17 BOLD:AEZ7204 OR974823
Ac ididae Callip amus wa enwylianus Pan el,
1896
INV00070 IBIOR009-17 BOLD:AEZ7204 OR974681
Ac ididae Callip amus wa enwylianus Pan el,
1896
INV11426 IBIOR550-22 BOLD:AEZ7204 OR974745
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV00071 IBIOR010-17 BOLD:AED8559 OR974762
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV00072 IBIOR011-17 BOLD:AED8559 OR974600
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV02506 IBIOR071-17 BOLD:AED8559 OR974536
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV02507 IBIOR072-17 BOLD:AED8559 OR974696
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV02532 IBIOR097-17 BOLD:AED8559 OR974673
Ac ididae Cho hippus apicalis (He ich-Schä e ,
1840)
INV02533 IBIOR098-17 BOLD:AED8559 OR974657
Ac ididae Cho hippus bino a us (Cha pen ie ,
1825)
INV03941 IBIOR331-22 BOLD:AAC5779 OR974519
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 5
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Cho hippus bino a us (Cha pen ie ,
1825)
INV05546 IBIOR197-22 BOLD:AAC5779 OR974508
Ac ididae Cho hippus bino a us (Cha pen ie ,
1825)
INV05547 IBIOR198-22 BOLD:AAC5779 OR974733
Ac ididae Cho hippus jacobsi Ha z, 19751 INV02535 IBIOR100-17 BOLD:AAC5779 OR974828
Ac ididae Cho hippus jacobsi Ha z, 19751 INV02536 IBIOR101-17 BOLD:AAC5779 OR974425
Ac ididae Cho hippus jacobsi Ha z, 19751 INV02537 IBIOR102-17 BOLD:AAC5779 OR974577
Ac ididae Cho hippus jucundus (Fische , 1853) INV03734 IBIOR323-22 BOLD:AER0035"OR974582
Ac ididae Cho hippus agans (E e smann,
1848)
INV00073 IBIOR012-17 BOLD:AAL3938 OR974714
Ac ididae Cho hippus agans (E e smann,
1848)
INV00074 IBIOR013-17 BOLD:AAL3938 OR974540
Ac ididae Cho hippus agans (E e smann,
1848)
INV02548 IBIOR111-17 BOLD:AAL3938 OR974820
Ac ididae Cho hippus agans (E e smann,
1848)
INV02704 IBIOR571-22 BOLD:AAL3938 OR974618
Ac ididae Cho hippus ye sini Ha z, 19751 INV05556 IBIOR207-22 BOLD:AAC5779 OR974682
Ac ididae Cho hippus ye sini Ha z, 19751 INV05557 IBIOR208-22 BOLD:AAC5779 OR974805
Ac ididae Docios au us genei (Ocskay, 1832) INV00075 IBIOR014-17 BOLD:ADU3247 OR974636
Ac ididae Docios au us genei (Ocskay, 1832) INV00076 IBIOR015-17 BOLD:ADU3247 OR974806
Ac ididae Docios au us hispanicus Bolí a ,
18981
INV00079 IBIOR018-17 BOLD:ADV6985 OR974787
Ac ididae Docios au us jagoi Sol ani, 1978 INV00077 IBIOR016-17 BOLD:ADT8663 OR974505
Ac ididae Docios au us jagoi Sol ani, 1978 INV00078 IBIOR017-17 BOLD:ADT8663 OR974567
Ac ididae Docios au us jagoi Sol ani, 1978 INV02549 IBIOR112-17 BOLD:ADT8663 OR974476
Ac ididae Docios au us jagoi Sol ani, 1978 INV03536 IBIOR308-22 BOLD:ADT8663 OR974516
Ac ididae Docios au us jagoi Sol ani, 1978 INV03540 IBIOR191-22 BOLD:ADT8663 OR974736
Ac ididae Docios au us jagoi Sol ani, 1978 INV03547 IBIOR309-22 BOLD:ADT8663 OR974585
Ac ididae Docios au us jagoi Sol ani, 1978 INV03556 IBIOR310-22 BOLD:ADT8663 OR974495
Ac ididae Docios au us jagoi Sol ani, 1978 INV03628 IBIOR313-22 BOLD:ADT8663 OR974808
Ac ididae Docios au us jagoi Sol ani, 1978 INV06726 IBIOR438-22 BOLD:ADT8663 OR974514
Ac ididae Docios au us jagoi Sol ani, 1978 INV07282 IBIOR464-22 BOLD:ADT8663 OR974807
6Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Docios au us jagoi Sol ani, 1978 INV07542 IBIOR473-22 BOLD:ADT8663 OR974544
Ac ididae Docios au us jagoi Sol ani, 1978 INV07619 IBIOR480-22 BOLD:ADT8663 OR974532
Ac ididae Docios au us jagoi Sol ani, 1978 INV11390 IBIOR546-22 BOLD:ADT8663 OR974482
Ac ididae Docios au us jagoi Sol ani, 1978 INV11391 IBIOR547-22 BOLD:ADT8663 OR974635
Ac ididae Docios au us jagoi Sol ani, 1978 INV11393 IBIOR549-22 BOLD:ADT8663 OR974646
Ac ididae Docios au us ma occanus (Thunbe g,
1815)
INV00080 IBIOR019-17 BOLD:AAX8673 OR974534
Ac ididae Docios au us ma occanus (Thunbe g,
1815)
INV00081 IBIOR020-17 BOLD:AAX8673 OR974574
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00083 IBIOR021-17 BOLD:ADJ5172 OR974465
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00084 IBIOR022-17 BOLD:ADJ5172 OR974592
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00085 IBIOR023-17 BOLD:ADJ5172 OR974659
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00086 IBIOR024-17 BOLD:ADJ5172 OR974625
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00087 IBIOR025-17 BOLD:ADJ5172 OR974559
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV00088 IBIOR026-17 BOLD:ADJ5172 OR974666
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV02579 IBIOR135-17 BOLD:ADJ5172 OR974674
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV02580 IBIOR136-17 BOLD:ADJ5172 OR974803
Ac ididae Eucho hippus elegan ulus Zeune ,
1940#
INV03557 IBIOR311-22 BOLD:ADJ5172 OR974428
Ac ididae Eyp epocnemis plo ans (Cha pen ie ,
1825)
INV05585 IBIOR236-22 BOLD:AAV4939 OR974729
Ac ididae Eyp epocnemis plo ans (Cha pen ie ,
1825)
INV05586 IBIOR237-22 BOLD:AAV4939 OR974676
Ac ididae Locus a mig a o ia (Linnaeus, 1758) INV02510 IBIOR075-17 BOLD:AAD9526 OR974584
Ac ididae Locus a mig a o ia (Linnaeus, 1758) INV02511 IBIOR076-17 BOLD:AAD9526 OR974821
Ac ididae Locus a mig a o ia (Linnaeus, 1758) INV05513 IBIOR141-19 BOLD:AAD9526 OR974698
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 7
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Miosci us wagne i (E e smann, 1859) INV05564 IBIOR215-22 BOLD:ABV1949 OR974436
Ac ididae Miosci us wagne i (E e smann, 1859) INV05565 IBIOR216-22 BOLD:ABV1949 OR974457
Ac ididae Mo phac is ascia a (Thunbe g, 1815) INV02560 IBIOR118-17 BOLD:ACR8091 OR974668
Ac ididae Mo phac is ascia a (Thunbe g, 1815) INV02569 IBIOR125-17 BOLD:ACR8091 OR974488
Ac ididae Oedaleus deco us (Ge ma , 1825) INV00091 IBIOR027-17 BOLD:AAE5868 OR974713
Ac ididae Oedaleus deco us (Ge ma , 1825) INV00092 IBIOR142-19 BOLD:AAE5868 OR974497
Ac ididae Oedipoda cae ulescens (Linnaeus,
1758)
INV00093 IBIOR028-17 BOLD:AAC9181 OR974732
Ac ididae Oedipoda cae ulescens (Linnaeus,
1758)
INV00094 IBIOR029-17 BOLD:AAC9181 OR974612
Ac ididae Oedipoda cae ulescens (Linnaeus,
1758)
INV02551 IBIOR114-17 BOLD:AAC9181 OR974535
Ac ididae Oedipoda cae ulescens (Linnaeus,
1758)
INV04033 IBIOR161-19 BOLD:AAC9181 OR974793
Ac ididae Oedipoda cha pen ie i Fiebe , 1853 INV00095 IBIOR030-17 BOLD:AAC9180 OR974452
Ac ididae Oedipoda cha pen ie i Fiebe , 1853 INV00096 IBIOR031-17 BOLD:AAC9180 OR974742
Ac ididae Oedipoda cha pen ie i Fiebe , 1853 INV02512 IBIOR077-17 BOLD:AAC9180 OR974548
Ac ididae Oedipoda cha pen ie i Fiebe , 1853 INV02513 IBIOR078-17 BOLD:AAC9180 OR974571
Ac ididae Oedipoda coe ulea Saussu e, 1884# INV07299 IBIOR465-22 BOLD:AEO7756 OR974419
Ac ididae Omoces us pan eli (Bolí a , 1887) INV00097 IBIOR032-17 BOLD:AAX0669 OR974413
Ac ididae Omoces us pan eli (Bolí a , 1887) INV00098 IBIOR033-17 BOLD:AAX0669 OR974740
Ac ididae Omoces us pan eli (Bolí a , 1887) INV00099 IBIOR034-17 BOLD:AAX0669 OR974780
Ac ididae Omoces us pan eli (Bolí a , 1887) INV00100 IBIOR035-17 BOLD:AAX0669 OR974546
Ac ididae Omoces us pan eli (Bolí a , 1887) INV00101 IBIOR036-17 BOLD:AAX0669 OR974726
Ac ididae Omoces us pan eli (Bolí a , 1887) INV02531 IBIOR096-17 BOLD:AAX0669 OR974790
Ac ididae Omoces us pan eli (Bolí a , 1887) INV10730 IBIOR265-22 BOLD:AAX0669 OR974565
Ac ididae Omoces us aymondi (Ye sin, 1863) INV02588 IBIOR178-22 BOLD:AEO7761 OR974430
Ac ididae Omoces us aymondi (Ye sin, 1863) INV02589 IBIOR179-22 BOLD:AEO7761 OR974511
Ac ididae Omoces us u ipes (Ze e s ed , 1821) INV02575 IBIOR131-17 BOLD:AAE1108 OR974755
Ac ididae Omoces us u ipes (Ze e s ed , 1821) INV02576 IBIOR132-17 BOLD:AAE1108 OR974773
Ac ididae Omoces us i idulus (Linnaeus, 1758) INV05555 IBIOR206-22 BOLD:AAE1108 OR974599
Ac ididae Pa acinema icolo (Thunbe g, 1815) INV06628 IBIOR432-22 BOLD:ACU5079 OR974730
1
1
1
1
1
1
1
8Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Ac ididae Pezo e ix gio nae (Rossi, 1794) INV00102 IBIOR037-17 BOLD:AEO7437"OR974583
Ac ididae Pezo e ix gio nae (Rossi, 1794) INV00103 IBIOR038-17 BOLD:AEO7437"OR974543
Ac ididae Pezo e ix gio nae (Rossi, 1794) INV02538 IBIOR103-17 BOLD:AEO7437"OR974709
Ac ididae Pezo e ix gio nae (Rossi, 1794) INV03278 IBIOR306-22 BOLD:AEO7436 OR974802
Ac ididae Pseudocho hippus pa allelus
(Ze e s ed , 1821)
INV03712 IBIOR319-22 BOLD:AAC3399 OR974527
Ac ididae Pseudocho hippus pa allelus
(Ze e s ed , 1821)
INV05554 IBIOR205-22 BOLD:AAC3399 OR974441
Ac ididae Sphingono us azu escens (Rambu ,
1838)
INV02556 IBIOR116-17 BOLD:AAJ3344 OR974768
Ac ididae Sphingono us azu escens (Rambu ,
1838)
INV02557 IBIOR172-22 BOLD:AAJ3344 OR974647
Ac ididae Sphingono us azu escens (Rambu ,
1838)
INV02594 IBIOR184-22 BOLD:AAJ3344 OR974570
Ac ididae Sphingono us azu escens (Rambu ,
1838)
INV06744 IBIOR439-22 BOLD:AAJ3344 OR974738
Ac ididae Sphingono us imi ans B unne on
Wa enwyl, 1882 #
INV02555 IBIOR171-22 BOLD:AER3084"OR974661
Ac ididae Sphingono us imi ans B unne on
Wa enwyl, 1882 #
INV08093 IBIOR244-22 BOLD:AER3084"OR974539
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV00105 IBIOR040-17 BOLD:AEI2886 OR974734
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV00106 IBIOR041-17 BOLD:AEI2886 OR974489
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV00107 IBIOR042-17 BOLD:AEI2886 OR974721
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV03709 IBIOR316-22 BOLD:AEI2886 OR974433
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV03711 IBIOR318-22 BOLD:AEI2886 OR974624
Ac ididae Sphingono us lluciapoma esi (De au ,
2005)
INV11392 IBIOR548-22 BOLD:AEI2886 OR974569
Ac ididae Sphingono us lusi anicus Ebne , 1941
#
INV08094 IBIOR245-22 BOLD:AEI2886 OR974699
1
1
1
1
1,
1,
1
1
1
1
1
1
1,
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 9
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae Conocephalus conocephalus
(Linnaeus, 1767)
INV06925 IBIOR446-22 BOLD:AEQ8460 OR974573
Te igoniidae Conocephalus conocephalus
(Linnaeus, 1767)
INV06944 IBIOR449-22 BOLD:AEQ8460 OR974561
Te igoniidae Conocephalus conocephalus
(Linnaeus, 1767)
INV06948 IBIOR453-22 BOLD:AEQ8460 OR974493
Te igoniidae Conocephalus uscus (Fab icius,
1793)
INV05583 IBIOR234-22 BOLD:AEQ8462 OR974609
Te igoniidae Conocephalus uscus (Fab icius,
1793)
INV06634 IBIOR435-22 BOLD:AEQ8462 OR974829
Te igoniidae Conocephalus uscus (Fab icius,
1793)
INV09078 IBIOR513-22 BOLD:AEQ8462 OR974579
Te igoniidae Cy aspis scu a a (Cha pen ie , 1825)# INV07949 IBIOR493-22 BOLD:AEQ8625 OR974541
Te igoniidae Cy aspis scu a a (Cha pen ie , 1825)# INV08776 IBIOR510-22 BOLD:AEQ8625 OR974639
Te igoniidae Cy aspis scu a a (Cha pen ie , 1825)# INV09030 IBIOR512-22 BOLD:AEQ8625 OR974656
Te igoniidae Dec icus albi ons (Fab icius, 1775)# INV00115 IBIOR049-17 BOLD:AEQ8654"OR974549
Te igoniidae Dec icus albi ons (Fab icius, 1775)# INV00116 IBIOR271-22 BOLD:AEQ8654"OR974550
Te igoniidae Dec icus albi ons (Fab icius, 1775)# INV02508 IBIOR073-17 BOLD:AEQ8654"OR974446
Te igoniidae Ephippige ida dilu a (Bolí a , 1878) # INV08107 IBIOR258-22 BOLD:AEP5780"OR974778
Te igoniidae Ephippige ida dilu a (Bolí a , 1878) # INV08108 IBIOR259-22 BOLD:AEP5780"OR974678
Te igoniidae Ephippige ida osae Ba a & Co eas,
2015 #
INV08109 IBIOR260-22 BOLD:AEP5781"OR974415
Te igoniidae Ince ana deco a a (Fiebe , 1853)# INV02577 IBIOR133-17 BOLD:AEO9013 OR974700
Te igoniidae Ince ana deco a a (Fiebe , 1853)# INV02578 IBIOR134-17 BOLD:AEO9013 OR974759
Te igoniidae Lep ophyes punc a issima (Bosc,
1792)
INV05562 IBIOR213-22 BOLD:ADT5783 OR974467
Te igoniidae Lluciapoma esius anapaulae
(Schmid , 2009) #
INV02573 IBIOR129-17 BOLD:AEO8290 OR974723
Te igoniidae Lluciapoma esius as u iensis (Bolí a ,
1898) #
INV03388 IBIOR307-22 BOLD:AEO6324"OR974819
Te igoniidae Lluciapoma esius as u iensis (Bolí a ,
1898) #
INV03952 IBIOR333-22 BOLD:AEO6324"OR974672
Te igoniidae Lluciapoma esius as u iensis (Bolí a ,
1898) #
INV05560 IBIOR211-22 BOLD:AEO8296 OR974648
1,
1,
1,2,
1,2,
1,
1,
1,
16 Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae Lluciapoma esius as u iensis (Bolí a ,
1898) #
INV05561 IBIOR212-22 BOLD:AEO8296 OR974697
Te igoniidae Lluciapoma esius s alii (Bolí a , 1877)
#
INV08100 IBIOR251-22 BOLD:AEO8295 OR974783
Te igoniidae Meconema halassinum (De Gee ,
1773)
INV03732 IBIOR159-19 BOLD:AAK7060 OR974440
Te igoniidae Meconema halassinum (De Gee ,
1773)
INV03944 IBIOR160-19 BOLD:AAK7060 OR974750
Te igoniidae Meconema halassinum (De Gee ,
1773)
INV04094 IBIOR165-19 BOLD:AAK7060 OR974507
Te igoniidae Me iop e a ambigua P au, 1986 # INV02600 IBIOR190-22 BOLD:AEO8174 OR974461
Te igoniidae Me iop e a ambigua P au, 1986 # INV05542 IBIOR193-22 BOLD:AEO8174 OR974767
Te igoniidae Neocallic ania ba osi Ba a , 2013 # INV08101 IBIOR252-22 BOLD:AEO7827"OR974485
Te igoniidae Neocallic ania ba osi Ba a , 2013 # INV08102 IBIOR253-22 BOLD:AEO7827"OR974563
Te igoniidae Neocallic ania lusi anica (Ai es &
Menano, 1916) #
INV08110 IBIOR261-22 BOLD:AEO7822 OR974578
Te igoniidae Neocallic ania lusi anica (Ai es &
Menano, 1916) #
INV08111 IBIOR262-22 BOLD:AEO7822 OR974435
Te igoniidae Neocallic ania lusi anica (Ai es &
Menano, 1916) #
INV08112 IBIOR263-22 BOLD:AEO7826"OR974827
Te igoniidae Neocallic ania miegii (Bolí a , 1873) # INV08103 IBIOR254-22 BOLD:AEO7823 OR974490
Te igoniidae Neocallic ania miegii (Bolí a , 1873) # INV08104 IBIOR255-22 BOLD:AEO7823 OR974513
Te igoniidae Neocallic ania sellige a me idionalis
(P au, 1996) #
INV02574 IBIOR130-17 BOLD:AEO7820"OR974631
Te igoniidae Neocallic ania sellige a me idionalis
(P au, 1996) #
INV04091 IBIOR354-22 BOLD:AEO7825 OR974528
Te igoniidae Neocallic ania sellige a me idionalis
(P au, 1996) #
INV07436 IBIOR470-22 BOLD:AEO7825 OR974462
Te igoniidae Neocallic ania sellige a sellige a
(Cha pen ie , 1825) #
INV06585 IBIOR429-22 BOLD:AEO7824"OR974724
Te igoniidae Neocallic ania se a a (Bolí a , 1885)
#
INV08105 IBIOR256-22 BOLD:AEO7821"OR974605
Te igoniidae Neocallic ania se a a (Bolí a , 1885)
#
INV08106 IBIOR257-22 BOLD:AEO7821"OR974589
1,
1,
1,
1,
1,2,
1,2,
1,
1,
1,
1,
1,
1,
1,
1,
1,
1,2,
1,2,
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 17
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae Odon u a glab icauda (Cha pen ie ,
1825)#
INV00117 IBIOR050-17 BOLD:AEO7751 OR974651
Te igoniidae Odon u a glab icauda (Cha pen ie ,
1825)#
INV02515 IBIOR080-17 BOLD:AEO7751 OR974587
Te igoniidae Odon u a glab icauda (Cha pen ie ,
1825)#
INV06518 IBIOR419-22 BOLD:AEO7751 OR974459
Te igoniidae Odon u a glab icauda (Cha pen ie ,
1825)#
INV06520 IBIOR420-22 BOLD:AEO7751 OR974804
Te igoniidae Odon u a glab icauda (Cha pen ie ,
1825)#
INV08771 IBIOR509-22 BOLD:AEO7751 OR974664
Te igoniidae Odon u a macphe soni Mo ales-
Agacino, 1943 #
INV05587 IBIOR238-22 BOLD:AEO7752 OR974608
Te igoniidae Odon u a macphe soni Mo ales-
Agacino, 1943 #
INV05588 IBIOR239-22 BOLD:AEO7752 OR974456
Te igoniidae Phane op e a nana Fiebe , 1853 INV02559 IBIOR117-17 BOLD:AEO7449 OR974643
Te igoniidae Phane op e a nana Fiebe , 1853 INV03968 IBIOR334-22 BOLD:AEO7449 OR974614
Te igoniidae Phane op e a nana Fiebe , 1853 INV03978 IBIOR335-22 BOLD:AEO7449 OR974650
Te igoniidae Phane op e a nana Fiebe , 1853 INV04008 IBIOR341-22 BOLD:AEO7449 OR974667
Te igoniidae Phane op e a nana Fiebe , 1853 INV04019 IBIOR344-22 BOLD:AEO7449 OR974798
Te igoniidae Phane op e a nana Fiebe , 1853 INV04021 IBIOR345-22 BOLD:AEO7449 OR974799
Te igoniidae Phane op e a nana Fiebe , 1853 INV04042 IBIOR349-22 BOLD:AEO7449 OR974444
Te igoniidae Phane op e a nana Fiebe , 1853 INV06063 IBIOR408-22 BOLD:AEO7449 OR974764
Te igoniidae Phane op e a nana Fiebe , 1853 INV06087 IBIOR412-22 BOLD:AEO7449 OR974416
Te igoniidae Phane op e a nana Fiebe , 1853 INV06558 IBIOR424-22 BOLD:AEO7449 OR974510
Te igoniidae Phane op e a nana Fiebe , 1853 INV06560 IBIOR425-22 BOLD:AEO7449 OR974758
Te igoniidae Phane op e a nana Fiebe , 1853 INV06629 IBIOR433-22 BOLD:AEO7449 OR974655
Te igoniidae Phane op e a nana Fiebe , 1853 INV09088 IBIOR515-22 BOLD:AEO7449 OR974469
Te igoniidae Phane op e a nana Fiebe , 1853 INV10234 IBIOR520-22 BOLD:AEO7449 OR974481
Te igoniidae Phane op e a nana Fiebe , 1853 INV10271 IBIOR522-22 BOLD:AEO7449 OR974586
Te igoniidae Phane op e a nana Fiebe , 1853 INV10339 IBIOR528-22 BOLD:AEO7449 OR974813
Te igoniidae Phane op e a nana Fiebe , 1853 INV11453 IBIOR555-22 BOLD:AEO7449 OR974449
Te igoniidae Phane op e a spa sa S ål, 1857# INV06918 IBIOR444-22 BOLD:AEO7448"OR974512
1,
1,
18 Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae Phane op e a spa sa S ål, 1857# INV06926 IBIOR447-22 BOLD:AEO7448"OR974518
Te igoniidae Phane op e a spa sa S ål, 1857# INV07540 IBIOR472-22 BOLD:AEO7448"OR974619
Te igoniidae Phane op e a spa sa S ål, 1857# INV07543 IBIOR474-22 BOLD:AEO7448"OR974771
Te igoniidae Pla ycleis a inis Fiebe , 1853 INV00122 IBIOR053-17 BOLD:AAH9443 OR974752
Te igoniidae Pla ycleis a inis Fiebe , 1853 INV02516 IBIOR081-17 BOLD:AAH9443 OR974816
Te igoniidae Pla ycleis albopunc a a (Goeze, 1778) INV03998 IBIOR338-22 BOLD:AEO6325 OR974784
Te igoniidae Pla ycleis albopunc a a (Goeze, 1778) INV05544 IBIOR195-22 BOLD:AEO6325 OR974442
Te igoniidae Pla ycleis albopunc a a (Goeze, 1778) INV06511 IBIOR417-22 BOLD:AEO6325 OR974815
Te igoniidae Pla ycleis albopunc a a (Goeze, 1778) INV07110 IBIOR458-22 BOLD:AEO6325 OR974626
Te igoniidae Pla ycleis in e media (Se ille, 1838) INV02518 IBIOR083-17 BOLD:AAE4790 OR974615
Te igoniidae Pla ycleis in e media (Se ille, 1838) INV10586 IBIOR532-22 BOLD:AAE4790 OR974728
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV00131 IBIOR058-17 BOLD:AEO6325 OR974479
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV00132 IBIOR059-17 BOLD:AEO6325 OR974756
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV00133 IBIOR060-17 BOLD:AEO6325 OR974450
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV00134 IBIOR061-17 BOLD:AEO6325 OR974690
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV02572 IBIOR128-17 BOLD:AEO6325 OR974581
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV05439 IBIOR399-22 BOLD:AAL4618 OR974658
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV05515 IBIOR403-22 BOLD:AEO6325 OR974429
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV05516 IBIOR404-22 BOLD:AAL4618 OR974716
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV07352 IBIOR468-22 BOLD:AEO6325 OR974679
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV07353 IBIOR469-22 BOLD:AAL4618 OR974671
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV10326 IBIOR527-22 BOLD:AEO6325 OR974468
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV10593 IBIOR534-22 BOLD:AAL4618 OR974814
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV11434 IBIOR551-22 BOLD:AEO6325 OR974630
Te igoniidae Pla ycleis sabulosa Azam, 1901# INV11452 IBIOR554-22 BOLD:AEO6325 OR974596
Te igoniidae Pla ys olus ma inezii (Bolí a , 1873)
#
INV02526 IBIOR091-17 BOLD:AEO7253 OR974818
Te igoniidae Pla ys olus ma inezii (Bolí a , 1873)
#
INV02527 IBIOR092-17 BOLD:AEO7253 OR974545
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV06068 IBIOR410-22 BOLD:AEO7102"OR974774
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV06069 IBIOR411-22 BOLD:AEO7102"OR974761
1,
1,
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 19
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV06609 IBIOR431-22 BOLD:AEO7102"OR974483
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV06631 IBIOR434-22 BOLD:AEO7102"OR974566
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV07175 IBIOR570-22 BOLD:AEO7102"OR974830
Te igoniidae P e olepis g alla a (Pan el, 1886)# INV07351 IBIOR467-22 BOLD:AEO7102"OR974809
Te igoniidae P e olepis lusi anica (Bolí a , 1900)
#
INV05574 IBIOR225-22 BOLD:AEO7101 OR974551
Te igoniidae P e olepis lusi anica (Bolí a , 1900)
#
INV07563 IBIOR479-22 BOLD:AEO7101 OR974437
Te igoniidae P e olepis spolia a Rambu , 1838 # INV06945 IBIOR450-22 BOLD:AEO7101 OR974831
Te igoniidae P e olepis spolia a Rambu , 1838 # INV08090 IBIOR241-22 BOLD:AEO7101 OR974424
Te igoniidae Pycnogas e cuculla us (Cha pen ie ,
1825) #
INV02590 IBIOR180-22 BOLD:AEO6978"OR974521
Te igoniidae Pycnogas e cuculla us (Cha pen ie ,
1825) #
INV05577 IBIOR228-22 BOLD:AEO6980"OR974622
Te igoniidae Pycnogas e jugicola G aells, 1851 # INV05563 IBIOR214-22 BOLD:AEO6979"OR974704
Te igoniidae Ruspolia ni idula (Scopoli, 1786) INV02561 IBIOR119-17 BOLD:AAJ7497 OR974654
Te igoniidae Ruspolia ni idula (Scopoli, 1786) INV04086 IBIOR163-19 BOLD:AAJ7497 OR974782
Te igoniidae Ruspolia ni idula (Scopoli, 1786) INV04089 IBIOR164-19 BOLD:AAJ7497 OR974529
Te igoniidae S e opleu us pseudolus (Bolí a ,
1878) #
INV02592 IBIOR182-22 BOLD:AEO6528"OR974515
Te igoniidae S e opleu us pseudolus (Bolí a ,
1878) #
INV02593 IBIOR183-22 BOLD:AEO6528"OR974620
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV00136 IBIOR062-17 BOLD:AER1298 OR974473
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV00137 IBIOR063-17 BOLD:AAF2021 OR974526
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV03387 IBIOR154-19 BOLD:AAF2021 OR974616
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV06544 IBIOR422-22 BOLD:AER1298 OR974556
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV06545 IBIOR423-22 BOLD:AAF2021 OR974522
Te igoniidae Tessellana essella a (Cha pen ie ,
1825)
INV09723 IBIOR583-22 BOLD:AER6209"OR974718
1,2,
1,2,
1,
1,
1,2,
1,2,
1,
1,
1,
20 Pina S e al
Family Taxa IBI code BOLD code BOLD BIN GenBank
Te igoniidae Te igonia i idissima (Linnaeus, 1758) INV02524 IBIOR089-17 BOLD:AAF1423 OR974547
Te igoniidae Te igonia i idissima (Linnaeus, 1758) INV02525 IBIOR090-17 BOLD:AAF1423 OR974531
Te igoniidae Te igonia i idissima (Linnaeus, 1758) INV05451 IBIOR401-22 BOLD:AAF1423 OR974520
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV02581 IBIOR137-17 BOLD:AER1533"OR974766
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV04118 IBIOR359-22 BOLD:AER1532 OR974598
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV05307 IBIOR396-22 BOLD:AER1532 OR974597
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV06570 IBIOR427-22 BOLD:AER1532 OR974439
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV07109 IBIOR457-22 BOLD:AER1532 OR974499
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV07232 IBIOR462-22 BOLD:AER1532 OR974725
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV07796 IBIOR490-22 BOLD:AER1531"OR974500
Te igoniidae Thy eono us bidens (Bolí a , 1887) # INV07805 IBIOR491-22 BOLD:AER1533"OR974471
Te igoniidae Tylopsis lili olia (Fab icius, 1793)# INV00140 IBIOR064-17 BOLD:AER2541 OR974552
Te igoniidae Tylopsis lili olia (Fab icius, 1793)# INV00141 IBIOR065-17 BOLD:AER2541 OR974797
Te igoniidae Tylopsis lili olia (Fab icius, 1793)# INV02520 IBIOR085-17 BOLD:AER2541 OR974792
Te igoniidae Tylopsis lili olia (Fab icius, 1793)# INV02521 IBIOR086-17 BOLD:AER2541 OR974422
Te igoniidae Tylopsis lili olia (Fab icius, 1793)# INV10591 IBIOR533-22 BOLD:AER2541 OR974502
T idac ylidae Xya ibe ica Gün he , 1990 # INV02567 IBIOR124-17 BOLD:AER2238 OR974754
T igonidiidae Na ula a e ni (Cos a, 1855)# INV02565 IBIOR123-17 BOLD:ACP3886 OR974642
T igonidiidae Na ula a e ni (Cos a, 1855)# INV02582 IBIOR138-17 BOLD:ACP3886 OR974795
T igonidiidae Nemobius syl es is (Bosc, 1792) INV02258 IBIOR144-19 BOLD:AAC3438 OR974524
T igonidiidae Nemobius syl es is (Bosc, 1792) INV02570 IBIOR126-17 BOLD:AER5488"OR974523
T igonidiidae Nemobius syl es is (Bosc, 1792) INV02571 IBIOR127-17 BOLD:AER5488"OR974691
T igonidiidae Nemobius syl es is (Bosc, 1792) INV03615 IBIOR157-19 BOLD:AAC3438 OR974826
T igonidiidae Nemobius syl es is (Bosc, 1792) INV03758 IBIOR328-22 BOLD:AAC3438 OR974675
T igonidiidae Nemobius syl es is (Bosc, 1792) INV03759 IBIOR329-22 BOLD:AAC3438 OR974769
T igonidiidae Nemobius syl es is (Bosc, 1792) INV07640 IBIOR484-22 BOLD:AEO7832"OR974684
T igonidiidae Nemobius syl es is (Bosc, 1792) INV07769 IBIOR489-22 BOLD:AEO7832"OR974746
T igonidiidae Nemobius syl es is (Bosc, 1792) INV08603 IBIOR504-22 BOLD:AER5488"OR974466
T igonidiidae P e onemobius lineola us (B ullé,
1835)#
INV03739 IBIOR325-22 BOLD:AEO6935 OR974731
1,
1,
1,
1,
1,
1,
1,
1,
1,
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 21
Family Taxa IBI code BOLD code BOLD BIN GenBank
T igonidiidae T igonidium cicindeloides Rambu ,
1838
INV02529 IBIOR094-17 BOLD:ACY3774 OR974533
T igonidiidae T igonidium cicindeloides Rambu ,
1838
INV02530 IBIOR095-17 BOLD:ACY3774 OR974420
T igonidiidae T igonidium cicindeloides Rambu ,
1838
INV07034 IBIOR456-22 BOLD:ACY3774 OR974412
T igonidiidae T igonidium cicindeloides Rambu ,
1838
INV10193 IBIOR519-22 BOLD:ACY3774 OR974786
The esul s show mul iple BINs o a ecognised species in se e al cases. Namely, h ee
BINs we e ob ained in each o he ecognised species gi en: An axius spinib achius,
Nemobius syl es is, Tessellana essella a and Thy eono us bidens. Two BINs we e
ob ained in each o he ollowing axa: G yllomo pha longicauda, G yllo alpa ineae,
Lluciapoma esius as u iensis, Neocallic ania lusi anica, Pezo e ix gio nae, Pycnogas e
cuculla us, Pla ycleis sabulosa and o he subspecies Neocallic ania sellige a me idionalis.
Ou da ase includes h ee BINs ob ained o he species An axius spinib achius, including
he i s sequences o he BIN BOLD:AER0568 om specimens collec ed in he Cas elo
B anco and Gua da Dis ic s. In a p e ious s udy, aimed a he phylogeog aphy o his
species in he Ibe ian Peninsula, wo di e en lineages o his species we e iden i ied, one
o which occu s along he Co dille a O e ana and he o he includes all o he popula ions
(Gu ié ez-Rod íguez e al. 2014). The gene ic di e si y ound in DNA ba codes in his
species highligh s he necessi y o a axonomic e ision. Addi ionally, in he amily
Te igoniidae, he specimen o he Neocallic ania genus collec ed in he Se úbal Peninsula
was mo phologically iden i ied as Neocallic ania lusi anica using he a ailable li e a u e
(Ba a 2007) and he unique BIN BOLD:AEO6978 was ob ained. Howe e , he e ision
conduc ed by Ba a (2013) poin ed ou unce ain ies ela ed o specimens collec ed in his
geog aphic a ea. Thus, ou esul s emphasise he need o u he wo k owa ds a be e
unde s anding o he axonomy o his genus in he Ibe ian Peninsula.
O he 119 species, 104 ha e di ec co espondence be ween mo phologic iden i ica ion
and BINs, lea ing 15 species in ol ed in BIN sha ing. In he sub amily Te igoniinae, wo
BINs we e ound o be sha ed by mo e han one species, he BIN BOLD:AEO6325 and he
BIN BOLD:AEO7101 was sha ed by Pla ycleis albopunc a a and Pla ycleis sabulosa and
by P e olepis lusi anica and P e olepis spolia a, espec i ely. In he sub amily
Gomphoce inae, he gene a ed sequence o Pseudocho hippus pa allelus sha ed BIN BO
LD:AAC3399 wi h sequences o Pseudocho hippus mon anus om Ge many, Uk aine and
No way. In og ession caused by hyb idisa ion is a well-s udied phenomenon in a eas
whe e bo h species occu in sympa y in Ge many (Hochki ch and Lemke 2011, Rohde e
al. 2015). These species a e mo phologically e y simila , bu ha e di e en ecological
equi emen s and bo h species occu in Spain, al hough Pseudocho hippus mon anus is
es ic ed o he no heas (Llucià-Poma es 2002). Addi ionally, in his sub amily, he e a e
o he Gomphoce inae g oups o species ha canno be iden i ied using he DNA ba code
22 Pina S e al
COI agmen . Sequences ob ained om ou specimens o Cho hippus bino a us,
Cho hippus jacobsi and Cho hippus ye sini sha ed he same BIN BOLD:AAC5779.
Mo eo e and in line wi h he esul s by Hawli schek e al. (2017), sequences ob ained as
Omoces us u ipes and Omoces us i idulus sha ed he same BIN. Likewise, in he
sub amily Oedipodinae, wo BINs we e sha ed by mo e han one species, namely he BIN
BOLD:AAJ3344 was sha ed by Sphingono us azu escens and Sphingono us nodulosus
and he BIN BOLD:AEI2886 was sha ed by Sphingono us lluciapoma esi and
Sphingono us lusi anicus. The success a e o DNA ba coding in he genus Sphingono us
was p e iously s udied by Dey e al. (2020). Thei esul s showed in ensi e sha ing o
iden ical o closely- ela ed DNA ba codes indica ing ha he single analysis o he COI
agmen is no su icien enough o di e en ia e many o he Sphingono us species.
a
b
c
d
e
Figu e 1.
Examples o he di e si y o species ha a e pa o he da ase o dis ibu ion da a and DNA
ba codes o Po uguese O hop e a 01.
a: Neocallic ania ba osi Ba a , 2013. Te igoniidae. Pho o by F ancisco Ba os. BIN URI BOL
D:AEO7827;
b: Tylopsis lili olia (Fab icius, 1793). Te igoniidae. Pho o by Sónia Fe ei a. BIN URI BOLD:AE
R2541;
c: Eug yllodes escale ae (Bolí a , 1894). G yllidae. Pho o by Sónia Fe ei a. BIN URI BOLD:A
EP5287;
d: G yllo alpa ineae Benne -Cla k, 1970. G yllo alpidae. Pho o by Síl ia Pina. BIN URI BOLD:
AEP5099; BOLD:AEP5100;
e: Pa amogoplis es o ini Llucià Poma es, 2015. Mogoplis idae. Pho o by Síl ia Pina. BIN URI
BOLD:AEO7942;
: Oecan hus dulcisonans Go ocho , 1993. Oecan hidae. Pho o by Síl ia Pina. BIN URI BOLD
:ACG7286.
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 23
This s udy shows ha DNA ba code sequences, based on he COI mi ochond ial gene
agmen , can be use ul in iden i ying Po uguese O hop e a o species le el. To ou
knowledge, his is he i s s udy o ocus on DNA ba coding o he O hop e a o de o he
Ibe ian Peninsula. I also highligh s se e al axonomic challenges ela ed o he ich auna
o his g oup and sugges s in ica ed phylogeog aphic p ocesses in he egion ha lead o
he di e si ica ion o se e al axa and high endemism le els in line wi h wha p e ious
a
b
c
d
e
Figu e 2.
Examples o he di e si y o species ha a e pa o he da ase o dis ibu ion da a and DNA
ba codes o Po uguese O hop e a.
a: T igonidium cicindeloides Rambu , 1838. T igonidiidae. Pho o by Síl ia Pina. BIN URI BOLD
:ACY3774;
b: Sphingono us lusi anicus Ebne , 1941. Ac ididae. Pho o by Síl ia Pina. BIN URI BOLD:AEI2
886;
c: Acinipe igna ii Llo en e del Mo al & P esa, 1983. Pamphagidae. Pho o by F ancisco Ba os.
BIN URI BOLD:AER2325;
d: Py gomo pha conica (Oli ie , 1791). Py gomo phidae. Pho o by Síl ia Pina. BIN URI BOLD:
AEO6938;
e: Pa a e ix me idionalis (Rambu , 1838). Te igidae. Pho o by Síl ia Pina. BIN URI BOLD:BO
LD:AAP5007;
: Xya ibe ica Gün he , 1990. T idac ylidae. Pho o by Sónia Fe ei a. BIN URI BOLD:AER2238
24 Pina S e al
s udies ha e ound (Ba anco 2004, Solé e al. 2018). Ou esul s cons i u e a i s s ep in
he cons uc ion o a DNA ba code da abase o a cu a ed e e ence collec ion o Ibe ian
(Po uguese) O hop e a species ha can be used in s udies whe e i is necessa y o
iden i y specimens ei he by DNA ba coding o DNA me aba coding.
P ojec desc ip ion
Ti le: The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om
Po ugal.
Pe sonnel: Ped o Beja (p ojec coo dina o ), Sónia Fe ei a ( axonomis and IBI manage ),
Síl ia Pina ( axonomis ), Joana Paupé io (IBI manage ), F ancisco Ba os ( axonomis ),
Cá ia Cha es (p ojec echnician), Filipa M.S. Ma ins (molecula biologis ), Joana Pin o,
Joana Ve íssimo (molecula biologis ), Vanessa Ma a (molecula biologis ).
S udy a ea desc ip ion: Con inen al Po ugal (Fig. 3).
Design desc ip ion: O hop e a specimens we e collec ed in he ield, mo phologically
iden i ied and DNA ba coded.
Funding: The p esen wo k was unded by Na ional Funds h ough FCT-Fundação pa a a
Ciência e a Tecnologia in he scope o he p ojec LA/P/0048/2020. InBIO Ba coding
Ini ia i e was unded by he Eu opean Union’s Ho izon 2020 Resea ch and Inno a ion
P og amme unde g an ag eemen No 668981 and he p ojec PORBIOTA—Po uguese
E-In as uc u e o In o ma ion and Resea ch on Biodi e si y (POCI-01-0145-
FEDER-022127), suppo ed by Ope a ional Thema ic P og am o Compe i i eness and
Figu e 3.
Map o he locali ies whe e O hop e a samples we e collec ed in con inen al Po ugal.
Po uguese dis ic s a e also ep esen ed.
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 25
lon The geog aphical longi ude (in decimal deg ees) o he geog aphic cen e o a loca ion.
ele Ele a ion o sampling si e (in me es abo e sea le el).
coun y The ull, unabb e ia ed name o he coun y whe e he o ganism was collec ed.
p o ince_s a e The ull, unabb e ia ed name o he p o ince ("Dis i o" in Po ugal) whe e he o ganism
was collec ed.
egion The ull, unabb e ia ed name o he municipali y ("Concelho" in Po ugal) whe e he
o ganism was collec ed.
exac si e Addi ional name/ ex desc ip ion ega ding he exac loca ion o he collec ion si e
ela i e o a geog aphic ele an landma k.
subspecies_ axID Subspecies axonomic nume ic code.
subspecies_name Subspecies name.
Acknowledgemen s
The p esen wo k was unded by Na ional Funds h ough FCT-Fundação pa a a Ciência e
a Tecnologia in he scope o he p ojec LA/P/0048/2020. The au ho s a e g a e ul o Ana
Ri a Gonçal es, José M. G osso-Sil a, Ma in Co ley, Ped o B i o Lopes, Ped o Sousa and
Rui And ade o collec ing specimens. InBIO Ba coding Ini ia i e was unded by he
Eu opean Union’s Ho izon 2020 Resea ch and Inno a ion P og amme unde g an
ag eemen No. 668981 and he p ojec PORBIOTA—Po uguese E-In as uc u e o
In o ma ion and Resea ch on Biodi e si y (POCI-01-0145- FEDER-022127), suppo ed by
Ope a ional Thema ic P og am o Compe i i eness and In e na ionaliza ion (POCI), unde
he PORTUGAL 2020 Pa ne ship Ag eemen , h ough he Eu opean Regional
De elopmen Fund (FEDER). Fieldwo k bene i ed om he p ojec PTDC/ BIA-BIC/
2203/2012-FCOMP-01-0124-FEDER-028289 by FEDER Funds h ough he Ope a ional
P og amme o Compe i i eness Fac o s–COMPETE and by Na ional Funds, EDP
Biodi e si y Chai , he p ojec “P omoção dos se iços de ecossis emas no Pa que Na u al
Regional do Vale do Tua: Con olo de P agas Ag ícolas e Flo es ais po Mo cegos” unded
by he Agência de Desen ol imen o Regional do Vale doTua and includes esea ch
conduc ed a he Long Te m Resea ch Si e o Baixo Sabo (LTER_EU_PT_002). The wo k
was pa ially Funded by Ho izon Eu ope unde he Biodi e si y, Ci cula Economy and
En i onmen call (REA.B.3); co- unded by he Swiss S a e Sec e a ia o Educa ion,
Resea ch and Inno a ion (SERI) unde con ac numbe 22.00173; and by he UK
Resea ch and Inno a ion unde he Depa men o Business, Ene gy and Indus ial
S a egy’s Ho izon Eu ope Gua an ee Scheme. SF and VM we e unded by he FCT
h ough he p og amme ‘S imulus o Scien i ic Employmen , Indi idual Suppo —3 d
Edi ion’ 2020.03526.CEECIND; 2020.02547.CEECIND). JV and FMSM by PhD g an s
(SFRH/BD/133159/2017; SFRH/BD/104703/2014) unded by FCT.
32 Pina S e al
Re e ences
• Ai es B, Menano H (1915) Ca álogo sinóp ico dos O óp e os de Po ugal. Re is a da
Uni e sidade de Coimb a 4: 451‑476.
• Ba a J (2007) Re isión axonómica de los Ephippige inae (O hop e a: Te igonioidea:
B adypo idae) de la Península Ibé ica e Islas Balea es. I. Géne os: Callic ania Bolí a ,
1898; Neocallic ania P au, 1996; Pla ys olus Bolí a , 1878 y Synephippius Na às, 1905.
Bole ín de la Sociedad En omológica A agonesa 40: 55‑118. URL: h p://sea-
en omologia.o g/Publicaciones/PDF/BOLN40/055_118BSEA40Ephippige inaeI.pd
• Ba a J (2013) Re isión de la iden idad de Neocallic ania se a a (Bolí a , 1885) y
desc ipción de dos áxones a ines: Neocallic ania se a a p aui ssp. n. y Neocallic ania
ba osi sp. n. (O hop e a, Te igoniidae, B adypo inae, Ephippige ini). Bole ín de la
Sociedad En omológica A agonesa 52: 1‑16. URL: h p://sea-en omologia.o g/PDF/
Bole in52/001016BSEA52O hop e aJBa a .pd
• Ba anco P (2004) Es udio del subgéne o Zape alop ila Go ocho & Llo en e, 2001 y
desc ipción de cua o nue as especies (Pe alop ila Pan el, 1890, O hop e a, G yllidae).
G aellsia 60 (1): 81‑93. h ps://doi.o g/10.3989/g aellsia.2004. 60.i1.194
• Bie inge G, Zulka KP, Milasowszky N, Saube e N (2013) Edge e ec o a pine
plan a ion educes d y g assland in e eb a e species ichness. Biodi e si y and
Conse a ion 22 (10): 2269‑2283. h ps://doi.o g/10.1007/s10531-013-0435-2
• Ca y I, Sampaio A, Sil a M, Mo ei a F, F anco AA, Ca y T (2018) Combining s able
iso ope analysis and con en ional echniques o imp o e knowledge o he die o he
Eu opean Rolle Co acias ga ulus. Ibis 161 (2): 272‑285. h ps://doi.o g/10.1111/ibi.
12625
• Chimeno C, Rulik B, Man in A, Kalinka G, Hölke F, Ba ano V (2023) Facing he
in ini y: ackling la ge samples o challenging Chi onomidae (Dip e a) wi h an in eg a i e
app oach. Pee J 11 h ps://doi.o g/10.7717/pee j.15336
• deWaa d J, Ra nasingham S, Zakha o E, Bo isenko A, S einke D, Tel e A, Pe ez KJ,
Sones J, Young M, Le esque-Beaudin V, Sobel C, Ab ahamyan A, Bessono K,
Blagoe G, deWaa d S, Ho C, I ano a N, Lay on KS, Lu L, Manjuna h R, McKeown JA,
Mil on M, Miskie R, Monkhouse N, Naik S, Nikolo a N, Pen insaa i M, P osse SJ,
Radulo ici A, S einke C, Wa ne C, Hebe PN (2019) A e e ence lib a y o Canadian
in e eb a es wi h 1.5 million ba codes, ouche specimens, and DNA samples.
Scien i ic Da a 6 (1). h ps://doi.o g/10.1038/s41597-019-0320-2
• Dey L, Hochki ch A, Husemann M (2020) Ve gleich on Einzel-Gen DNA Ba coding und
Mul i-Gen Analysen in de Ga ung Sphingono us (Ac ididae: Oedipodinae).
ARTICULATA 35: 47‑59. URL: h ps://dg o-a icula a.de/downloads/a icula a/
a icula a_35_2020/A icula a_35_2020_Dey_e _al.pd
• Fe ei a S, Fonseca N, Ege e B, Paupé io J, Galha do M, Oxel el F, A es a S, Ma ins
F, A che J, Co ley M, Penado A, Pina S, Ja man S, Beja P (2018) Deli e able 4.2
(D4.2): P o ocol o building and o ganising e e ence collec ions o DNA sequences,
En Me aGen p ojec (G an Ag eemen No 668981). Zenodo h ps://doi.o g/10.5281/
zenodo.3515967
• Folme O, Black M, Hoeh W, Lu z R, V ijenhoek R (1994) DNA p ime s o ampli ica ion
o mi ochond ial cy och ome c oxidase subuni I om di e se me azoan in e eb a es.
Molecula ma ine biology and bio echnology 3 (5): 294‑9.
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 33
• Gay án Á, Be gs en J, Canelo T, Pé ez‐Izquie do C, San o o M, Bonal R (2020) DNA
Ba coding and geog aphical scale e ec : The p oblems o unde sampling gene ic
di e si y ho spo s. Ecology and E olu ion 10 (19): 10754‑10772. h ps://doi.o g/10.1002/
ece3.6733
• GBIF (2023) GBIF Home Page. h ps://doi.o g/10.15468/39omei. Accessed on:
2023-12-15.
• Geige MF, As in JJ, Bo sch T, Bu kha d U, G obe P, Hand R, Hausmann A, Hohbe g
K, K ogmann L, Lu z M, Monje C, Miso B, Mo iniè e J, Mülle K, Pie sch S, Quand D,
Rulik B, Scholle M, T aunspu ge W, Haszp una G, Wägele W (2016) How o ackle
he molecula species in en o y o an indus ialized na ion—lessons om he i s
phase o he Ge man Ba code o Li e ini ia i e GBOL (2012–2015). Genome 59 (9):
661‑670. h ps://doi.o g/10.1139/gen-2015-0185
• Gu ié ez-Rod íguez J, Ba anco P, Ga cía-Pa ís M (2014) Phylogeog aphy o An axius
spinib achius (O hop e a: Te igoniidae) e eals mul iple lineages ac oss Ibe ian
Peninsula wi h e idences o a new axon. Ac as del XVI Cong eso Ibé ico de
En omología. XVI Cong eso Ibé ico de En omología, Badajoz, 2014.
• Hawli schek O, Mo iniè e J, Lehmann GUC, Lehmann AW, K op M, Dunz A, Glaw F,
De cha oen M, Schmid S, Hausmann A, Szucsich NU, Cae ano‐Wyle SA, Haszp una
G (2017) DNA ba coding o c icke s, ka ydids and g asshoppe s (O hop e a) om
Cen al Eu ope wi h ocus on Aus ia, Ge many and Swi ze land. Molecula Ecology
Resou ces 17 (5): 1037‑1053. h ps://doi.o g/10.1111/1755-0998.12638
• Hebe PN, Cywinska A, Ball S, deWaa d J (2003) Biological iden i ica ions h ough
DNA ba codes. P oceedings o he Royal Socie y o London. Se ies B: Biological
Sciences 270 (1512): 313‑321. h ps://doi.o g/10.1098/ spb.2002.2218
• Hochki ch A, Lemke I (2011) Asymme ic ma e choice, hyb idiza ion, and hyb id i ness
in wo sympa ic g asshoppe species. Beha io al Ecology and Sociobiology 65 (8):
1637‑1645. h ps://doi.o g/10.1007/s00265-011-1174-6
• Hochki ch A, Nie o A, C iado MG, Cálix M, B aud Y, Buzze i F, Chobano D, Odé B,
Asensio JJP, Willemse L, Zuna-K a ky T, Vega PB, Bushell M, Clemen e ME, Co eas J,
Dusoulie F, Fe ei a S, Fon ana P, Ga cía MD, Helle K, Io gu I, I ko ić S, Ka i V,
Kleuke s R, K iš ín A, Lemonnie -Da cemon M, Lemos P, Massa B, Monne a C,
Papapa lou K, P unie F, Pushka T, Roes i C, Ru schmann F, Şi in D, Skejo J,
Szö ényi G, Tzi kalli E, Vedenina V, Domenech JB, Ba os F, Tapia PC, De au B,
Fa mann T, Gomboc S, Gu ié ez-Rod íguez J, Holuša J, Illich I, Ka jalainen S,
Kočá ek P, Ko suno skaya O, Liana A, López H, Mo in D, Olmo-Vidal JM, Puskás G,
Sa i sky V, S alling T, Tumb inck J (2016) Eu opean Red Lis o g asshoppe s, c icke s
and bush-c icke s. Luxembou g: Publica ions O ice o he Eu opean Union. URL:
h ps://po - als.iucn.o g/lib a y/si es/lib a y/ iles/documen s/RL-4-021.pd
• IUCN (2023) The IUCN Red Lis o Th ea ened Species. Ve sion 2023-1. h ps://
www.iucn edlis .o g. Accessed on: 2023-12-14.
• Janzen D, Hallwachs W (2011) Joining In en o y by Pa a axonomis s wi h DNA
Ba coding o a La ge Complex T opical Conse ed Wildland in No hwes e n Cos a
Rica. PLoS ONE 6 (8). h ps://doi.o g/10.1371/jou nal.pone.0018123
• Kasalo N, Skejo J, Husemann M (2023) DNA ba coding o pygmy hoppe s— he i s
comp ehensi e o e iew o he BOLD Sys ems’ Da a Shows P omise o Species
Iden i ica ion. Di e si y 15 (6). h ps://doi.o g/10.3390/d15060696
34 Pina S e al
• Lemos P, Lock K, Odé B, Kleuke s R (2016) Six O hop e a species new o he auna o
Po ugal (O hop e a: Te igoniidae, G yllidae, Te igidae, Ac ididae). Bole ín de la
Sociedad En omológica A agonesa 58: 115‑122. URL: h p://www.sea-socios.com/
Bole ines/PDF/Bole in58/115-122BSEA58.pd
• Llucià-Poma es D (2002) Re isión de los o óp e os (Insec a: O hop e a) de Ca aluña
(España). Monog a ías de la Sociedad A agonesa de En omología 7: 1‑226. URL:
h p://sea-en omologia.o g/PDF/
MSEA7O hop e aCa alu%C3%B1aResolucionMode ada.pd
• Mon ei o E, Ba os F, Augus o AM, Oli ei a AEd, Mi al o MO, Fe ei a S (2016) New
eco ds o Pe alop ila (Pe alop ila) e mini Go ocho & Llo en e, 2001 and G yllomo pha
(G yllomo phella) uclensis Pan el, 1890 om con inen al Po ugal (O hop e a,
G yllidae). Bole ín de la Sociedad En omológica A agonesa 58: 225‑226. URL: h p://
www.sea-socios.com/Bole ines/PDF/Bole in58/225-226BSEA58.pd
• Pina S, Vasconcelos S, Reino L, San ana J, Beja P, Sánchez-Oli e J, Ca y I, Mo ei a
F, Fe ei a S (2017) The O hop e a o Cas o Ve de Special P o ec ion A ea (Sou he n
Po ugal): new da a and conse a ion alue. ZooKeys 691: 19‑48. h ps://doi.o g/
10.3897/zookeys.691.14842
• Ra nasingham S, He be PN (2007) BOLD: The Ba code o Li e Da a Sys em (h p://
www.ba codingli e.o g). Molecula Ecology No es 7 (3): 355‑364. h ps://doi.o g/10.1111/
j.1471-8286.2007.01678.x
• Rohde K, Hau Y, Weye J, Hochki ch A (2015) Wide p e alence o hyb idiza ion in wo
sympa ic g asshoppe species may be shaped by hei ela i e abundances. BMC
E olu iona y Biology 15 (1). h ps://doi.o g/10.1186/s12862-015-0460-8
• Shok alla S, Po e T, Gibson J, Dobosz R, Janzen D, Hallwachs W, Golding GB,
Hajibabaei M (2015) Massi ely pa allel mul iplex DNA sequencing o specimen
iden i ica ion using an Illumina MiSeq pla o m. Scien i ic Repo s 5 (1). h ps://doi.o g/
10.1038/s ep09687
• Solé J, A nedo MA, Ba a J, Enguídanos A, Pujade-Villa J, F anch M, Cadiach O,
Aguila F, Ga cía G, Pascual R (2018) Range, popula ion s uc u e and mo phological
cha ac e iza ion o he small ange endemic bush-c icke Lluciapoma esius pan eli
(O hop e a: Te igoniidae: B adypo inae). Jou nal o Insec Conse a ion 22: 659‑674.
h ps://doi.o g/10.1007/s10841-018-0092-6
• Valdez E, C yan P (2013) Insec P ey Ea en by Hoa y Ba s (Lasiu us cine eus) P io o
Fa al Collisions wi h Wind Tu bines. Wes e n No h Ame ican Na u alis 73 (4): 516‑524.
h ps://doi.o g/10.3398/064.073.0404
• an Klink R, Augus T, Bas Y, Bodesheim P, Bonn A, Fossøy F, Høye T, Jongejans E,
Menz MM, Mi aldo A, Roslin T, Roy H, Ruczyński I, Schigel D, Schä le L, Shea d J,
S enningsen C, Tschan G, Wäldchen J, Zizka VA, Ås öm J, Bowle D (2022) Eme ging
echnologies e olu ionise insec ecology and moni o ing. T ends in Ecology & E olu ion
37 (10): 872‑885. h ps://doi.o g/10.1016/j. ee.2022.06.001
• Vasconcelos S, Pina S, Reino L, Beja P, Mo ei a F, Sánchez-Oli e J, Ca y I, Fa ia J,
Ro enbe y J, San ana J (2019) Long- e m consequences o ag icul u al policy
decisions: How a e o es s plan ed unde EEC egula ion 2080/92 a ec ing biodi e si y
20 yea s la e ? Biological Conse a ion 236: 393‑403. h ps://doi.o g/10.1016/j.biocon.
2019.05.052
The InBIO Ba coding Ini ia i e Da abase: DNA ba codes o O hop e a om ... 35
Supplemen a y ma e ials
Suppl. ma e ial 1: DS-IBIOR01 InBIO Ba coding Ini ia i e - O hop e a 01 -
Specimen de ails
Au ho s: Síl ia Pina, Joana Paupé io, F ancisco Ba os, Cá ia Cha es, Filipa M.S. Ma ins,
Joana Pin o, Joana Ve íssimo, Vanessa Ma a, Ped o Beja, Sónia Fe ei a
Da a ype: Reco d in o ma ion - specimen da a
B ie desc ip ion: The ile includes in o ma ion abou all eco ds in BOLD o he DS-IBIOR01
InBIO Ba coding Ini ia i e - O hop e a 01 lib a y. I con ains collec ion and iden i ica ion da a.
Download ile (161.27 kb)
Suppl. ma e ial 2: IBI – O hop e a 01 lib a y - Specimen de ails - Da win Co e
S anda d
Au ho s: Síl ia Pina, Joana Paupé io, F ancisco Ba os, Cá ia Cha es, Filipa M.S. Ma ins,
Joana Pin o, Joana Ve íssimo, Vanessa Ma a, Ped o Beja, Sónia Fe ei a
Da a ype: Specimen da a eco ds in he Da win Co e S anda d o ma .
B ie desc ip ion: The ile includes in o ma ion abou all eco ds in BOLD o he DS-IBIOR01
InBIO Ba coding Ini ia i e - O hop e a 01. I con ains collec ion and iden i ica ion da a.
Download ile (191.09 kb)
Suppl. ma e ial 3: IBI - O hop e a 01 lib a y - DNA sequences
Au ho s: Síl ia Pina, Joana Paupé io, F ancisco Ba os, Cá ia Cha es, Filipa M.S. Ma ins,
Joana Pin o, Joana Ve íssimo, Vanessa Ma a, Ped o Beja, Sónia Fe ei a
Da a ype: Specimen genomic da a, DNA sequences.
B ie desc ip ion: COI sequences in as a o ma . Each sequence is iden i ied by he BOLD
P ocessID, species name, gene ic ma ke name and GenBank accession numbe , all sepa a ed
by a e ical ba . The da a a e as downloaded om BOLD.
Download ile (307.09 kb)
36 Pina S e al
