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UDC 547.856.1:631.811.98
BREEDING AND SELECTION OF SALT-TOLERANT COTTON
(GOSSYPIUM HIRSUTUM L.) GENOTYPES USING BIOACTIVE
QUINAZOLINONE DERIVATIVES
Tu obo a Nasiba Nu ullaye na., Sai kulo Foziljon E gashe ich
Tashken s a e ag a ian uni e si y
Email: [email p o ec ed]
Esbe geno Uzaqbe gen Embe gen uli
S uden o he ka akalpaks an ins i u e o ag icul u e and ag o echnology
DOI: h ps://doi.o g/10.5281/zenodo.17536768
Abs ac : This s udy ocuses on he syn hesis and biological e alua ion o ecologically
sa e and bioac i e 2-amino-6-oxopu ine de i a i es as po en ial plan g ow h egula o s.
En i onmen ally iendly syn he ic me hods we e applied using mild eac ion condi ions and
non- oxic sol en s o ob ain high-pu i y and s able compounds. Spec oscopic and
ch oma og aphic analyses con i med hei molecula s uc u e and composi ion. The biological
expe imen s e ealed ha 2-amino-6-oxopu ine de i a i es signi ican ly s imula e seed
ge mina ion, oo and shoo de elopmen , and enhance plan esis ance o d ough and salini y
s ess. Among he syn hesized compounds, iodine- and cobal -con aining de i a i es
demons a ed he highes biological ac i i y. The esul s indica e ha hese compounds can se e
as eco-sa e bios imulan s in sus ainable ag icul u e, p omo ing plan p oduc i i y and
en i onmen al p o ec ion.
Key wo ds: 2-amino-6-oxopu ine, eco-sa e syn hesis, bioac i e compounds, plan g ow h
egula o s, sus ainable ag icul u e, s ess ole ance.
In oduc ion. In he mode n e a o sus ainable ag icul u al de elopmen , he
demand o en i onmen ally sa e and biologically ac i e plan g ow h egula o s is
s eadily inc easing. Excessi e use o chemical e ilize s, pes icides, and g ow h
s imulan s has led o soil deg ada ion, en i onmen al con amina ion, and nega i e
e ec s on human heal h. As a esul , scien is s a ound he wo ld a e sea ching o
g een al e na i es ha can imp o e plan p oduc i i y wi hou ha ming ecosys ems.
Among such subs ances, pu ine de i a i es, especially 2-amino-6-oxopu ine, ha e
a ac ed signi ican a en ion due o hei mul i unc ional biological p ope ies.
2-Amino-6-oxopu ine, also known as hypoxan hine, is a na u al pu ine base
p esen in nucleic acids and plays a i al ole in a ious biochemical p ocesses
such as DNA and RNA syn hesis, ni ogen me abolism, and ene gy ans e . I s
de i a i es a e known o exhibi a wide ange o biological ac i i ies, including
an ioxidan , enzyma ic, and g ow h-p omo ing e ec s. The e o e, hei use as
po en ial eco-sa e bios imulan s in ag icul u e can con ibu e o imp o ing plan
g ow h, enhancing esis ance o en i onmen al s ess, and main aining ecological
balance.
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The impo ance o de eloping ecologically sa e syn hesis me hods lies in
minimizing he en i onmen al impac du ing chemical p oduc ion. T adi ional
syn he ic ou es o en in ol e oxic sol en s and ca alys s, which cause was e
gene a ion and pollu ion. In con as , g een chemis y app oaches — such as
sol en - ee syn hesis, aqueous eac ions, and he use o mild ca alys s — ensu e
ha he esul ing p oduc s a e bo h high-quali y and en i onmen ally iendly. This
s udy ocuses on he c ea ion o new 2-amino-6-oxopu ine de i a i es using eco-
sa e syn he ic me hods, ollowed by es ing hei biological e ec i eness on
ag icul u al c ops.
Table 1. Physicochemical and Biological Cha ac e is ics o Syn hesized 2-
Amino-6-Oxopu ine De i a i es
Compound
Name
Molecula
Fo mula
Molecula
Weigh
(g/mol)
Mel ing
Poin (°C)
Solubili y
(mg/mL,
H₂O)
Biological
Ac i i y*
2-Amino-6-
oxopu ine
C5H5N5O
151.12
249–251
0.85
++
9-Me hyl-2-
amino-6-
oxopu ine
C6H7N5O
165.15
235–238
1.02
+++
8-E hyl-2-amino-
6-oxopu ine
C7H9N5O
179.18
228–231
0.94
++
8-Iodo-2-amino-
6-oxopu ine
C5H4IN5O
297.02
258–261
0.57
++++
2-Amino-6-
oxopu ine–Co(II)
complex
[Co(C5H5N5O)₂]
355.23
276–279
0.63
++++
Mo eo e , coo dina ion compounds o 2-amino-6-oxopu ine wi h ansi ion
me als (such as cobal , zinc, and i on) we e also syn hesized o enhance hei
biological ac i i y and s abili y. Me al ions can signi ican ly in luence he
physicochemical p ope ies o pu ine de i a i es, imp o ing hei solubili y,
bioa ailabili y, and ca aly ic e iciency in biochemical p ocesses. Such complexes
ha e demons a ed imp o ed e iciency in p omo ing seed ge mina ion and oo
elonga ion in p e ious s udies.
The o e all goal o he esea ch is o syn hesize a new gene a ion o eco-
sa e, bioac i e 2-amino-6-oxopu ine-based compounds ha can eplace
con en ional syn he ic s imulan s. The ob ained p epa a ions a e expec ed o
imp o e c op g ow h and p oduc i i y unde s ess condi ions such as d ough ,
salini y, and nu ien de iciency, hus con ibu ing o sus ainable and
en i onmen ally esponsible a ming p ac ices.
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The da a p esen ed in Table 1 indica e ha s uc u al modi ica ion o he
pu ine nucleus no ably in luences bo h he solubili y and biological e iciency o
he compounds. The in oduc ion o iodine and cobal a oms in o he molecula
amewo k enhances hei bioac i i y, sugges ing a s ong co ela ion be ween
chemical s uc u e and physiological unc ion. These indings p o ide a scien i ic
ounda ion o he p ac ical applica ion o 2-amino-6-oxopu ine de i a i es as eco-
iendly g ow h egula o s in mode n ag icul u al sys ems.
Conclusions. The conduc ed esea ch con i med ha 2-amino-6-oxopu ine
and i s de i a i es can be syn hesized h ough en i onmen ally sa e and e icien
me hods. The applica ion o g een chemis y p inciples — including mild eac ion
condi ions, non- oxic sol en s, and ecyclable ca alys s — made i possible o
ob ain high-pu i y, s able compounds wi h minimal en i onmen al impac .
Spec oscopic (IQ, UB) and ch oma og aphic analyses e i ied hei s uc u al
in eg i y and homogenei y, ensu ing he eliabili y o expe imen al esul s.
Biological es ing on a ious ag icul u al c ops demons a ed ha hese
compounds possess signi ican g ow h-p omo ing and s ess-p o ec i e
p ope ies. In pa icula , iodine- and cobal -con aining de i a i es exhibi ed he
highes biological ac i i y, s imula ing seed ge mina ion, oo elonga ion, and
pho osyn he ic p ocesses unde d ough and salini y condi ions. These e ec s a e
likely due o hei abili y o egula e enzyma ic eac ions and enhance ni ogen
me abolism wi hin plan issues. The co ela ion be ween chemical s uc u e and
bioac i i y e ealed ha subs i u ion a he 8 h o 9 h posi ion o he pu ine ing
inc eases physiological e iciency, while me al coo dina ion u he imp o es
solubili y and plan up ake. As a esul , he syn hesized compounds can be
e ec i ely used as eco- iendly bios imulan s o enhance plan p oduc i i y and
esilience wi hou causing en i onmen al ha m.
O e all, he esul s sugges ha 2-amino-6-oxopu ine-based p epa a ions
ep esen a p omising new class o sus ainable g ow h egula o s o mode n
ag icul u e. Thei use can educe dependence on syn he ic e ilize s and
pes icides, imp o e soil heal h, and ensu e high c op yields unde en i onmen ally
esponsible managemen sys ems. Fu u e s udies should ocus on la ge-scale ield
ials, o mula ion de elopmen , and de ailed in es iga ion o molecula
mechanisms go e ning hei biological ac i i y.
104
Li e a u e:
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3-indolyl ace ic acid in he “ambe ” plan s g ades phaseolus au eus. Академические
исследования в современной науке, 1(17), 263-267.
2. Sai kulo , F., Qilichye a, N., Abdullaye , B., An a o , A., & E gashe a, M. (2022).
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