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UDC: 547.859:577
CREATION OF ECOLOGICALLY SAFE AND BIOACTIVE 2-AMINO-6-
OXOPURINE PREPARATIONS
Qliche a Na osa Kabulo na
Mas e ’s deg ee Sama kand S a e Uni e si y
Nasimo Hasan Mu ado ich
Accosia e p o esso Sama kand s a e uni e si y
Email: [email p o ec ed]
Baxaua dino a Miyassa Gay a din qizi
S uden o he ka akalpaks an ins i u e o ag icul u e and ag o echnologies
Email: [email p o ec ed]
Tu imbe o Mu a bay Shamshe o ich
Doc o o Philosophy (PhD) in Ag icul u al Sciences,
Ac ing Associa e P o esso o he Depa men o Ag icul u e, Selec ion and Seed P oduc ion o
Ag icul u al C ops
[email p o ec ed]
DOI: h ps://doi.o g/10.5281/zenodo.17537925
Abs ac : The s udy ocuses on c ea ing ecologically sa e and biologically ac i e 2-amino-
6-oxopu ine p epa a ions. En i onmen ally iendly syn hesis me hods we e de eloped using mild
eac ion condi ions and non- oxic sol en s. The ob ained compounds we e analyzed by
spec oscopic and ch oma og aphic echniques o ensu e pu i y and s abili y. Biological es ing
on plan s e ealed ha hese p epa a ions signi ican ly s imula e g ow h and imp o e esis ance
o en i onmen al s ess. The eco-sa e syn hesis and bioac i i y o he p oduc s sugges hei
po en ial use as g ow h-p omo ing agen s in sus ainable ag icul u e. The esea ch highligh s he
impo ance o pu ine-based compounds in enhancing plan me abolism and p oduc i i y unde
ecological condi ions.
Key wo ds: 2-amino-6-oxopu ine, eco- iendly syn hesis, bioac i e compounds, plan
g ow h s imula ion, sus ainable ag icul u e.
In oduc ion
In ecen yea s, he sea ch o ecologically sa e and biologically ac i e
compounds capable o enhancing plan g ow h and esis ance o ad e se
en i onmen al ac o s has gained inc easing a en ion in sus ainable ag icul u e. One
o he p omising di ec ions in his ield is he syn hesis o pu ine-based compounds,
pa icula ly 2-amino-6-oxopu ine (hypoxan hine) de i a i es, which play a i al ole
in plan me abolism, enzyme ac i i y, and nucleic acid biosyn hesis. These
compounds a e na u ally occu ing ni ogenous bases ha o m he s uc u al co e
o nucleo ides, and hei syn he ic analogues can ac as bio egula o s in luencing
a ious physiological and biochemical p ocesses in plan s.
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Mode n ag icul u al p ac ices equi e new, en i onmen ally iendly g ow h
s imulan s ha can eplace syn he ic chemical e ilize s and pes icides. Excessi e
use o hese chemicals has caused se e e soil deg ada ion, en i onmen al pollu ion,
and accumula ion o oxic esidues in c ops. The e o e, he de elopmen o eco-sa e
bios imulan s based on na u al o biocompa ible compounds is an u gen scien i ic
and p ac ical issue. In his ega d, 2-amino-6-oxopu ine and i s de i a i es a e o
special in e es due o hei mul i unc ional biological ac i i y, including plan
g ow h egula ion, s ess ole ance imp o emen , and pho osyn he ic e iciency
enhancemen [1-5].
The chemical s uc u e o 2-amino-6-oxopu ine p o ides nume ous
possibili ies o s uc u al modi ica ion and o ma ion o new bioac i e de i a i es.
Th ough subs i u ion a speci ic posi ions o he pu ine ing, new compounds can be
ob ained wi h imp o ed solubili y, s abili y, and biological e iciency. Recen
ad ances in coo dina ion chemis y ha e also enabled he de elopmen o me al–
pu ine complexes, which o en show highe biological ac i i y han ee ligands.
Such complexes can ac as ca aly ic cen e s, in luencing plan me abolic pa hways
and p omo ing be e nu ien u iliza ion.
Ecologically sa e syn hesis o pu ine de i a i es in ol es using mild eac ion
media, non- oxic ca alys s, and sol en - ee o aqueous eac ion sys ems. These
g een chemis y app oaches minimize en i onmen al pollu ion and imp o e he
yield and quali y o bioac i e p oduc s. In addi ion, mode n analy ical echniques,
such as in a ed spec oscopy (IR), ul a iole - isible (UV-Vis) spec oscopy, and
ch oma og aphic me hods (HPLC, GC), make i possible o p ecisely de e mine he
pu i y and s uc u e o syn hesized compounds.
In he p esen s udy, a se ies o 2-amino-6-oxopu ine-based compounds we e
syn hesized using en i onmen ally benign me hods. Thei s uc u al cha ac e is ics
we e con i med spec oscopically, and hei biological ac i i y was es ed on a ious
c op plan s unde con olled condi ions. The esul s demons a e ha hese
compounds no only enhance plan g ow h bu also imp o e s ess ole ance unde
d ough and salini y condi ions. Such e ec s a e a ibu ed o hei abili y o ac i a e
enzyma ic sys ems ela ed o ni ogen me abolism and oxida i e s ess egula ion.
Fu he mo e, 2-amino-6-oxopu ine de i a i es a e known o in luence plan
ho monal balance, pa icula ly cy okinins and auxins, which a e esponsible o cell
di ision and elonga ion. By s imula ing hese ho monal pa hways, he compounds
con ibu e o inc eased oo and shoo biomass, accele a ed ge mina ion, and
imp o ed c op yield. The combina ion o high biological e iciency and ecological
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sa e y makes hese subs ances p omising candida es o sus ainable ag icul u al
echnologies.
Thus, he s udy o 2-amino-6-oxopu ine-based p epa a ions p o ides aluable
insigh s in o he design o nex -gene a ion bioac i e agen s. Thei po en ial
applica ion in ag icul u e could lead o he de elopmen o g een echnologies aimed
a main aining soil e ili y, enhancing c op p oduc i i y, and educing
en i onmen al isks associa ed wi h chemical e ilize s able 1.
№
Compound Name
Molecula
Fo mula
Molecula
Weigh
(g/mol)
Mel ing
Poin (°C)
Solubili y
in Wa e
(mg/mL)
Biological
Ac i i y*
1
2-Amino-6-
oxopu ine
C5H5N5O
151.12
249–251
0.85
++
2
2-Amino-6-
oxopu ine-9-
me hyl
C6H7N5O
165.15
235–238
1.02
+++
3
2-Amino-6-
oxopu ine-8-e hyl
C7H9N5O
179.18
228–231
0.94
++
4
2-Amino-6-
oxopu ine-8-iodo
C5H4IN5O
297.02
258–261
0.57
++++
5
2-Amino-6-
oxopu ine-Co(II)
complex
[Co(C5H5N5O)2]
355.23
276–279
0.63
++++
Table 1. S uc u al and physicochemical p ope ies o syn hesized 2-
amino-6-oxopu ine de i a i es
Conclusions. The conduc ed esea ch has shown ha ecologically sa e and
biologically ac i e 2-amino-6-oxopu ine p epa a ions can be e ec i ely
syn hesized using mild eac ion condi ions and en i onmen ally iendly eagen s.
Spec oscopic and ch oma og aphic analyses con i med ha he ob ained
compounds possess high pu i y and chemical s abili y, making hem sui able o
biological es ing. Expe imen s pe o med on a ious ag icul u al plan s e ealed
ha hese compounds signi ican ly s imula e seed ge mina ion, oo and shoo
de elopmen , and inc ease plan biomass unde bo h no mal and s ess condi ions.
Pa icula ly, iodine- and cobal -con aining de i a i es demons a ed he highes
bios imulan ac i i y, enhancing plan s’ esis ance o d ough and salini y s ess.
This is likely ela ed o hei abili y o ac i a e enzyma ic sys ems esponsible o
ni ogen me abolism and oxida i e s ess egula ion. The ecological sa e y o hese
compounds, combined wi h hei s ong physiological ac i i y, indica es hei
po en ial as g een g ow h egula o s in mode n sus ainable ag icul u e.
Thus, 2-amino-6-oxopu ine-based p epa a ions can se e as a new gene a ion o
eco-sa e plan s imulan s ha p omo e g ow h, inc ease p oduc i i y, and educe
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dependence on syn he ic e ilize s and chemical agen s. Fu u e esea ch should
ocus on s udying hei mechanisms o ac ion, long- e m e ec s on soil and plan
heal h, and he de elopmen o e icien applica ion echnologies o ag icul u al
p ac ice.
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исследования в современной науке, 1(17), 263-267.
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