Pilo Node P ofile
The CNB-CSIC Pilo Node esponds o he challenges
and possibili ies o he S uc u al Biology communi y
ela ed o da a alida ion in e ms o quali y by o e ing
he c yo-EM Valida ion Repo Se ice (VRS), a modula
and open alida ion g ading sys em ha quali ies
s uc u e maps a six di e en le els depending on he
in o ma ion a ailable o assess i .
• AAI
{in p og ess}
CNB-CSIC Pilo Node
P o iding o he s uc u al biology communi y essen ial da a and
se ices o assess he quali y o c yo-elec on Mic oscopy da a
FACTSHEET FOR:
Pilo Co e Se ices
Use s o y a -a-glance
Th oughou he c yo-EM da a cycle, nume ous s eps
in ol e da a gene a ion, beginning wi h sample
p epa a ion and acquisi ion a he c yo-EM acili ies and
culmina ing in he econs uc ion o he olume map and
a omic models ha ep esen he na u e o a
mac omolecule a ge . As he numbe o maps deposi ed
in public da abases de e mined by c yo-EM is quickly
g owing, i becomes c ucial o complemen c yo-EM da a
wi h quali y- ela ed da a a he la es s age o he c yo-EM
da a cycle. Indeed, no all da a ha e he same quali y,
al hough i is no easily disce nible.
In his con ex , he c yo-EM Valida ion Repo Se ice
(VRS) will be o e ed o he s uc u al biology communi y.
Use s could upload and alida e hei da a ia an
open-access websi e, whe e an au oma ed Scipion
wo k low will handle he da a analysis.
This se ice elimina es he need o use s o download
da ase s, ins all so wa e o se up he en i onmen . A
comp ehensi e PDF epo summa ising he e alua ion
will be a ailable o download.
EOSC Beyond Co e Inno a ion Sandbox
A p e-p oduc ion en i onmen whe e a la ge ange o use s, om esea che s o node ope a o s,
can es he la es de elopmen s o he EOSC co e.
I ac s as a Fede a o Node o e ing Co e Fede a ing Capabili ies o he Pilo Nodes.
S uc u al biology communi y: esea ches, pha maceu ical companies, c yo-EM acili ies, e c.
Pilo Exchange Se ices
• C yo-EM Valida ion Repo Se ice (VRS)
Use ul Links
In eg a ed Co e Fede a ing Capabili ies om
he Co e Inno a ion Sandbox
• AAI
• Deploymen Se ice
• Use Space (planned)
• O de Managemen (planned)
• Helpdesk (planned)
• Moni o ing (planned)
•Da a T ans e (planned)
• PID (planned)
In eg a ed se ices om o he Pilo Nodes
• Ins uc -ERIC AAI
CNB-CSIC Pilo Node:
h ps://www.eosc-beyond.eu/pilo /cnb-csic
(c yo-EM) Valida ion Repo Se ice:
h ps://biocomp.cnb.csic.es/EMValida ionSe ice/
Abou EOSC Beyond
www.eosc-beyond.eu
The ambi ion o EOSC Beyond is o suppo he
g ow h o he EOSC in e ms o in eg a ed p o ide s
and ac i e use s by p o iding new Co e echnical
solu ions ha allow de elope s o scien i ic appli)
ca ion en i onmen s o easily compose a di e se
po olio o EOSC Resou ces, o e ing hem as
in eg a ed capabili ies o esea che s.
CC BY 4.0
EOSC Beyond is unded by he
Eu opean Union G an Ag eemen
Numbe 101131875
Value o he Node pilo ing ac i i ies
The C yo-EM Valida ion Repo Se ice (VRS) can be
used in wo main ways: o alida e p i a e use da a
o o assess exis ing public s uc u es deposi ed in
he EMDB and PDB. In he i s case, s uc u al
biology esea che s can gain aluable insigh s in o
he quali y o hei da a, along wi h ecommenda ions
on how o imp o e hei esul s.
This p ocess elimina es he need o use s o ins all
any so wa e o ha e access o high-pe o mance
compu ing esou ces o pe o m hese alida ions. In
he second case, he se ice also con ibu es o
g ea e openness and ep oducibili y wi hin he
s uc u al biology communi y.
Nex s eps
In he upcoming mon hs he VRS se ice will be ully in eg a ed wi h AAI, Use Space and O de Managemen
and la e wi h he Helpdesk, Moni o ing and PID co e se ice oo.
DOI: h ps://doi.o g/10.5281/zenodo.17729045
CNB-CSIC Node in eg a ions wi h
he Co e Inno a ion Sandox and
o he Pilo Nodes
