SUPPLEMENTARY INFORMATION
A new analy ical me hod o de e mine ace le el concen a ions o pha maceu icals in
in luen was ewa e : A ool o moni o human use pa e ns
Nú ia Fon anals1*, E a Pocu ull1, Rosa Mon es2, I ia González-Ma iño3, Se gio San ana-Vie a4,
Manuel Mi ó5, And eu Rico6,7, Sa a Rod íguez-Mozaz8,9, F ancesc Bo ull1, José Beni o
Quin ana2, Rosa Ma ia Ma cé1
1Uni e si a Ro i a i Vi gili. Depa men o Analy ical Chemis y and O ganic Chemis y.
Ta agona, Spain
2Depa men o Analy ical Chemis y, Nu i ion and Food Sciences. Ins i u e o Resea ch in
Chemical and Biological Analysis (IAQBUS). Uni e sidade de San iago de Compos ela. San iago
de Compos ela, Spain
3Depa men o Analy ical Chemis y, Nu i ion and B oma ology, Facul y o Chemical Sciences,
Uni e si y o Salamanca, Salamanca, Spain
4Ins i u o de Es udios Ambien ales y Recu sos Na u ales (i-UNAT), Uni e sidad de Las Palmas
de G an Cana ia, Las Palmas de G an Cana ia, Spain
5FI-TRACE g oup, Depa men o Chemis y, Uni e si y o he Balea ic Islands. Palma de
Mallo ca, Spain
6IMDEA Wa e Ins i u e, Science and Technology Campus o he Uni e si y o Alcalá, Alcalá de
Hena es, Spain
7Ca anilles Ins i u e o Biodi e si y and E olu iona y Biology, Uni e si y o Valencia, Pa e na,
Spain
8Ca alan Ins i u e o Wa e Resea ch (ICRA-CERCA), Gi ona, Spain
9Uni e si y o Gi ona (UdG), Gi ona, Spain
*Co esponding au ho email: [email p o ec ed]
Figu e S1. S uc u e o he s udied compounds.
Table S1. Main cha ac e is ics o he was ewa e ea men plan s (WWTPs) included in his s udy.
WWTP
Ci y
Region
Popula ion
Co e ing
he ci y (%)
A e age
low
(m3/day)
Sampling da e
Sampling modea
Mad id 1
Mad id
Mad id
Communi y
352188
30
75102
19-25/10/2022
T (450 mL/60 min)
Mad id 2
Mad id
Mad id
Communi y
727176
30
63393
19-25/10/2022
T (100 mL/60 min)
Ta agona
Ta agona
Ca alonia
115567
100
24690
13-19/04/2021
T (400 mL/30 min)
Reus
Reus
Ca alonia
108953
100
17533
13-19/04/2021
Flow
Palma de
Mallo ca
Palma de
Mallo ca
Balea ic
Islands
472319
100 (Palma
ci y,
excep ing
Palma
Beach a ea)
65538
21-26/04/2021
T (100 mL/15 min)
Las
Palmas de
G an
Cana ia
Las
Palmas de
G an
Cana ia
Cana y
Islands
477857
100
37009
14-19/04/2021
T (100 mL/30 min)
a Sampling mode: T = Time p opo ional ( olume sampled/ equency o sampling), Flow = Flow p opo ional
Figu e S2. Ex ac ed ion ch oma og am o a s anda d solu ion using he op imized sepa a ion
condi ions.
mo phine
a enolol
codeine
amadol
pen oxi ylline
omep azole
enla axine
azadone
sul ame hoxazole
que iapine
ca bamazepine
oxazepam
me hadone
losa an
diazepam
beza ib a e
diclo enac
Table S2. Valida ion pa ame e s.
Ins umen al
linea ange
(µg/L)
R2 IQLs
(µg/L)
IDLs
(µg/L)
%R
app
a
P ecision
in aday
(%RSD,
n=5) a
P ecision
in e day
(%RSD,n=5)
a
MQLs
(ng/L)
MDLs
(ng/L)
Mo phine
1-1000
0.9943
1
0.5
87
5.2
10.9
12
6
A enolol
5-1000
0.9957
5
2
87
6.3
7.6
58
23
Codeine
1-250
0.9997
1
0.2
57
6.1
8.3
18
5
T amadol
0.5-1000
0.9999
0.5
0.05
46
5.1
9.0
11
1
Pen oxi ylline
1-1000
0.9930
1
0.1
122
4.3
10.5
8
0.8
Que iapine
0.5-1000
0.9969
0.5
0.05
39
5.7
12.3
13
1
Omep azole
1-500
0.9998
1
0.5
42
6.7
14.1
24
12
Sul ame hoxazole
1-1000
0.9994
1
0.5
62
4.6
11.0
16
8
Venla axine
0.1-500
0.9986
0.1
0.02
43
5.2
10.7
2
0.5
T azodone
0.5-1000
0.9963
0.5
0.1
54
3.2
9.8
9
2
Losa an
0.8-1000
0.9997
0.8
0.2
101
5.8
6.4
8
2
Ca bamazepine
0.5-1000
0.9918
0.5
0.1
97
5.8
12.1
5
1
Oxazepam
1-1000
0.9999
1
0.5
117
4.3
13.9
8
4
Diazepam
0.8-500
0.9993
0.8
0.2
68
5.5
9.2
12
3
Me hadone
0.1-1000
0.9979
0.1
0.02
100
4.6
8.6
1
0.2
Beza ib a e
0.8-1000
0.9998
0.8
0.2
126
6.1
10.9
6
2
Diclo enac
2-1000
0.9998
2
0.5
139
5.8
12.3
14
4
a spiked a 1000 ng/L.
