First record of Tanycypris alfonsi Nagler et al., 2014 (Crustacea, Ostracoda) from India

he jou nal o biodi e si y da a NOTES ON GEOGRAPHIC DISTRIBUTION 884 Academic edi o : Samee Padhye Recei ed: 29 July 2025 Accep ed: 23 Sep embe 2025 Published: 1 Oc obe 2025 Copy igh © The au ho s. This is an open‑access a icle dis ibu ed unde e ms o he C ea i e Commons A ibu ion License (A ibu ion 4.0 In e na ional – CC BY 4.0) Abs ac . The eshwa e os acod Tanycyp is al onsi Nagle e al., 2014, was o iginally desc ibed om Ge ‑ many, whe e i was conside ed an alien species. We epo he i s con i med occu ence o T. al onsi Nagle e al., 2014 in India, whe e i was ound in a na u al habi a , which indica es ha India lies wi hin i s na i e geog aphic ange. Key wo ds. Cyp ice cinae, dis ibu ion, eshwa e os acod, Maha ash a, mic oc us aceans Khai na RR, Shinde YS (2025) Fi s eco d o Tanycyp is al onsi Nagle e al., 2014 (C us acea, Os acoda) om India. Check Lis 21 (5): 884–889. h ps://doi.o g/10.15560/21.5.884 INTRODUCTION The sub amily Cyp ice cinae is one o he di e se sub amilies o he amily Cyp ididae, which belongs o he class Os acoda (subphylum C us acea), cu en ly comp ises 172 species wo ldwide (Meisch e al. 2024). This sub amily o os acod has a dis inc se o diagnos ic cha ac e s o iden i ica ion, which we e e ised by Sa a enalin on and Ma ens (2009). The leading cha ac e is ic o iden i ying he sub amily is he p esence and posi ion o T iebel’s loop on he caudal amus a achmen . Cu en ly, he genus Tanycyp is T iebel, 1959 is classi ied wi hin he ibe Nealecyp idini (Sa a enalin on and Ma ens 2009) and comp ises six species—T. al onsi Nagle , Geis & Ma zke‑Ka asz, 2014, T. cen a Chang, Lee & Smi h, 2012, T. eugenkemp i Sa a enalin on, 2017, T. madagasca ensis (Mülle , 1898), T. gonia Smi h & Ozawa, 2024, T. pellucida (Klie, 1932), T. siamensis Sa‑ a enalin on & Ma ens, 2009—and one in aspeci ic axon, Tanycyp is pellucida a . saloica (Aka o a, 1950); hese a e dis ibu ed ac oss h ee zoogeog aphical egions: A o opical, O ien al, and Palaea c ic (Meisch e al. 2024; Smi h e al. 2024). Tanycyp is al onsi was disco e ed by Nagle e al. (2014) in he g eenhouse o he bo anical ga den in Mu‑ nich, Ge many. Since hen, his species has been epo ed om only h ee loca ions, in Sou h Ko ea (Smi h e al. 2014), eas e n China (Ma and Yu 2018), and Japan (Smi h 2023). We epo he i s con i med occu ence o T. al onsi in India, whe e i was ound in a na u al habi a , which indica es ha India lies wi hin i s na i e geog aphic ange. METHODS Samples we e collec ed om a pool nea he Tapi Ri e in he Jalgaon Dis ic o Maha ash a, India (21°05′17.9″N, 075°58′34.1″E). Samples we e collec ed using a simple hand ne wi h a mesh size o 150 µm, placed in 100 ml con aine s, and p ese ed in 4% o maldehyde solu ion. In he labo a o y, he samples we e insed unde ap wa e and p ese ed in 70% alcohol. The specimens we e isola ed, hei al es sep‑ a a ed, and dissec ion was pe o med using ungs en needles unde a s e eomic oscope (Magnus MS24). The sepa a ed al es we e moun ed on a s ub o examina ion using a scanning elec on mic oscope (JEOL Analy ical Scanning Elec on Mic oscope). The dissec ed so pa s we e moun ed in poly inyl lac ophenol and illus a ed using a came a lucida a ached o a Law ence and Mayo LM‑52‑1802 mic oscope. These d awings we e digi ized using Inkscape . 1.3.2. Images we e p ocessed using GIMP . 3.0.4. A dis ibu ion map was c ea ed using QGIS . 3.22.6 (QGIS 2022). The e minology used o so ‑pa ana omy and chae o axy was de i ed om B oodbakke and Daniel‑ opol (1982), e ised chae o axy o A2 by Ma ens (1987) and Meisch (2000). The specimens we e deposi ed 21 (5) · h ps://doi.o g/10.15560/21.5.884 21 (5): 884–889 Fi s eco d o Tanycyp is al onsi Nagle e al., 2014 (C us acea, Os acoda) om India Renuka Rajend a Khai na 1, Yugandha Sa ish Shinde1 1 Aqua ic Biology Resea ch Labo a o y, Depa men o Zoology, PES’s Mode n College o A s, Science and Comme ce (Au onomous), Shi ajinaga , Pune-411005, India (A ilia ed wi h Sa i ibai Phule Pune Uni e si y, Pune-7) Co esponding au ho : Yugandha S. Shinde (yugandh[email p o ec ed]om) Check Lis 21 (5) · h ps://doi.o g/10.15560/21.5.884 Khai na and Shinde · Fi s eco d o Tanycyp is al onsi om India 885 in he c us acean collec ion o he Zoological Su ey o India, Wes e n Regional Cen e, Pune, unde he accession numbe s ZSI‑WRC C.2650, ZSI‑WRC C.2660. Abb e ia ions o al es and so pa s. RV = igh al e; LV = le al e; limbs. A1 = i s an enna; A2 = second an enna; Md = mandibula; Mxl = maxillula; Tl = i s ho acopod; T2 = second ho acopod; T3 = hi d ho acopod; CR = caudal amus; CRa = caudal amus a achmen . RESULTS Tanycyp is al onsi Nagle , Geis & Ma zke-Ka asz, 2014 Figu e 1A–C New eco d. INDIA — Maha ash a • Jalgaon Dis ic , pool nea Tapi i e ; 21°05′17.9″N, 075°58′34.1″E; 210m ele a ion; 0.5 m dep h; 18.XI.2023; Renuka Khai na leg.; 3♀, 70% alcohol, ZSI‑WRC C.2650 & ZSI‑WRC C.2660. Desc ip ion. The ca apace (Figu e 1A–C) is 1280–1320 µm long, and 600–620 µm high. The ca apace is yellow, and he eye is p ominen . The su ace o he al es bea spa sely dis ibu ed hai s and a oo ‑ ile‑like s uc u e a he pos e io end (Figu e 1A). The ca apace is elonga e in he la e al iew, hin in he do sal iew, and has he g ea es wid h a he middle. The ends o he al es a e ounded, he do sal ma gin is s aigh , and he en al ma gin is sligh ly conca e in on o he middle. The LV and RV ha e b oad inne lamellae a he an e io and pos e io ends, wi hou an inne lis . A g oo e is p esen along he an e o en al ma gin in he LV (Figu e 1B), whe eas he RV lacks a g oo e (Figu e 1B). The inne ma gins o bo h al es a e ounded a he an e io end bu sligh ly cu ed a he pos e o en al end. A1 (Figu e 2A) has a la ge, 7‑segmen ed i s segmen and bea s a long do so‑apical senso y Wou e s o gan, one sho do sal apical se a, and wo long subapical en al dis al se ae. The second segmen has a long en al Rome o gan and a sho do sal se a. The hi d segmen has one se a on each side, and he ou h segmen has wo long do so‑apical and one sho en al apical se a. The i h segmen has i e se ae ( wo long do so‑apical, one long (medial), and wo sho en al se ae); one en al se a eaches sligh ly beyond he nex segmen , and he second eaches almos he mid‑leng h o he nex segmen . The six h segmen has ou long se ae and one sho hin se a ha does no each he dis al end o he e minal segmen . The se en h segmen has wo long se ae, one senso y se a ya, and one sho accompanying se a. Aes he asc ya and he accompanying se a a e almos o same leng h. A2 (Figu e 2B): he i s p o opodal segmen has h ee se ae (one p oximally, wo mo e dis ally), and he second has one e y long basal se a si ua ed a he cen e . The exopod was educed wi h h ee se ae (one long and wo sho ). The i s endopodal segmen bea s he senso y aes he asc Y, which consis s o h ee dis ally loca ed segmen s, one long hai y se a, and i e long na a o y se ae, which each he dis al end o he claws, wi h one sho accompanying se a. On he secondsegmen do sally, he e a e wo and Figu e 1. Tanycyp is al onsi Nagle e al., (2014). A. La e al iew o ca apace. B. Le al e in e nal iew. C. Righ in e nal iew. Scale ba s: 200 µm. Check Lis 21 (5) · h ps://doi.o g/10.15560/21.5.884 Khai na and Shinde · Fi s eco d o Tanycyp is al onsi om India 886 on en ally, ou unequally long ‑se ae ( 1– 4). A he dis al end o he same segmen , h ee se a ed claws G1–G3 (wi h G2 smalle han he o he s), h ee long z‑se ae (z1–z3), and a small y2 eaching almos he dis al end o he e minal segmen a e p esen . The e minal segmen bea s claw GM and Gm, aes he asc y3, and se a g. The g is longe han y3. Md‑coxa (Figu e 2C): as shown in he igu e. Md‑palp (Figu e 2D): 4‑segmen ed. The i s segmen was b oad, bea ing wo long, se ula ed s1 and s2 se ae and one sho , hin, alpha (α) se a. The second segmen has h ee ypes o se ae on he en al side: one sho , conical, densely hai y be a (β) se a; one medium‑leng h hai y se a; and h ee long, slim se ae on he en al side. Do sally, i bea s h ee se ae o di e en leng hs ( wo long eaching beyond he claws o he e minal segmen and one sho ). The hi d segmen has ou do so‑subapical se ae ( wo long and wo sho ). Ve o‑dis ally, one long and one sho se a. A he dis al end o he same segmen , h ee long se ae, including one hai y gamma (γ) se a, we e p esen , which was wice he leng h o he e minal segmen . The e minal segmen bea s h ee claws and h ee se ae. Mxl (Figu e 2E): palp 2‑segmen ed. The basal segmen bea s one medial se a ( eaching beyond he mid‑leng h o he nex segmen ), one subapical se a, and i e apical se ae o di e en leng hs ha ex end‑ ed beyond he dis al end o he e minal segmen . The e minal segmen is elonga e and bea s h ee claws and h ee se ae. The hi d endi e bea s one s ongly se a ed and ano he sligh ly se a ed “Zahnbo s en” ( oo h b is les). T1 (Figu e 3A): he p o opodi e has wo a‑se ae, one b and d se a, a he dis al end o he segmen ; 14 se ae a e p esen , all o which a e hai y dis ally. The endopodi e bea s h ee se ae o unequal leng h. T2 (Figu e 3B): he basal segmen bea s d1 and d2 (wi h d1 longe han d2). The i s segmen bea s e Figu e 2. Tanycyp is al onsi Nagle e al., (2014). A. Fi s an enna. B. Second an enna. C. De ail o las segmen o second an enna. D. Mandibula coxa. E. Mandibula palp. F. Te minal segmen o Mandibula palp. G. Maxillula. Scale ba s: A–C, E–G = 50 µm; D = 100 µm. Check Lis 21 (5) · h ps://doi.o g/10.15560/21.5.884 Khai na and Shinde · Fi s eco d o Tanycyp is al onsi om India 887 se ae, and he second segmen is ma kedly sepa a ed, bea ing a middle se a and dis al g se a. The ou h segmen was sho , bea ing se a h1 and h3 (o almos equal size), and a long, cu ed claw se a ed a he dis al end. T3 (Figu e 3C, D): he basal segmen bea s long d1, d2, and dp se ae (wi h d2 longe han d1). The i s segmen has a long e se a. The second segmen medially bea s se ae ( eaching he dis al end o he e mi‑ nal segmen ). The e minal segmen bea s an apical pince h2, a sho h1 se a, and a subapical h3 se a. The CR (Figu e 3E) was symme ical wi h s ongly se a ed Ga and Gp claws. The Sa se a is smoo h and ex ends beyond he mid‑leng h o he claw Ga. Se a Sa is longe han claw Gp. Sp se a is sho and sligh ly se a ed. The en al ma gin is se a ed along almos i s en i e leng h. CR a achmen (Figu e 3F): he ibbles loop is a he middle, he do sal b anch is hin, elonga e, and ben a he dis al end, and he en al b anch is wide, s ubby, and ounded. Taxonomic ema ks. Ou Indian specimens esemble T. al onsi as o iginally desc ibed, bu hey also show signi ican mo phological di e ences in he shape o he ca apace, A1, and CR. The in e sec ion o pos e o‑do sal ma gin in Ge man specimens is sligh ly dep essed while in Indian specimens his ma gin is smoo hly cu ed. E en he shape o he inne ma gin is di e en in bo h popula ions. In he o iginal desc ip ion o he species (Nagle e al. 2014), he Wou e s o gan was no ed as being ela i ely small and inconspicuous. In con as , ou Indian specimens ha e a ma kedly elonga e Wou e s o gan, app oxima ely he same leng h as he Rome o gan. Addi ionally, he do sal se a o ou specimens is conside ably longe . I a ises p oximally a he base o he Wou e s o gan, in con as o he o iginal desc ip ion in which his se a is sho and subapical. Fu he mo e, he Sp se a on he CR in Ge man specimens is slende and smoo h, whe eas in Indian and Chinese specimens (Ma and Yu 2018), i has a b oade base and a b ush‑like dis al end. DISCUSSION The genus Tanycyp is is known om h ee zoogeog aphical egions. Tanycyp is al onsi was o iginally de‑ sc ibed om Ge many, whe e i is conside ed an alien species (Nagle e al. 2014), and i has since been epo ed om small bodies o wa e bodies on Jeju Island, Sou h Ko ea (Smi h e al. 2014), in eas e n China (Ma and Yu 2018), and in he O ien al pa o he Nansei Islands, Japan (Smi h 2023). He e, we epo T. al onsi o he i s ime om India, whe e i was ound in a i e ine ecosys em. This species was p e iously known Figu e 3. Tanycyp is al onsi Nagle e al., (2014). A. Fi s ho acopod. B. Second ho acopod. C. Thi d ho acopod. D. Pince o gan. E. Caudal amus. F. Caudal amus a achmen . Scale ba s: A–C, E, F = 100 µm; D = 50 µm. Check Lis 21 (5) · h ps://doi.o g/10.15560/21.5.884 Khai na and Shinde · Fi s eco d o Tanycyp is al onsi om India 888 only om egions no h o he T opic o Cance ; howe e , he new eco d om India is he i s om sou h o his la i ude (Figu e 4). We hypo hesise ha T. al onsi is possibly an Eas Asian species which has been in oduced o Ge many, o ha he Indian popula ion (ou eco d iden i ied as T. al onsi) may ep esen a dis inc , undesc ibed spe‑ cies. Gene ic analysis o hese popula ions would sol e he axonomic unce ain y. Un il molecula da a a e a ailable, we p o isionally e e o he Indian specimens as T. al onsi. Un il now, o he genus Tanycyp is, only T. pellucida has been epo ed om India (Ha shey and Sh i as a 1983). A epo o T. pellucida by Shinde (2012) was e‑examined and i s iden i ica ion is co ec ed he e as T. al onsi. ACKNOWLEDGEMENTS We exp ess ou since e g a i ude o he P incipal o Mode n College, Pune‑5, o p o iding he acili ies o his esea ch. We a e also hank ul o he Head, Depa men o Physics, Sa i ibai Phule Pune Uni e si y, o p o iding access o he SEM acili y. YSS is hank ul o he au ho i ies o PES’s Mode n College, Pune‑5 o i‑ nancial assis ance (SEED money 2023–2024). RRK g a e ully acknowledges he Maha ash a S a e Biodi e si y Boa d (MSBB) o g an ing pe mission o collec samples (MSBB/DESK 5/1194 2022–23) and is g a e ul o he Maha ma Jyo iba Phule Resea ch Fellowship o inancial suppo o esea ch wo k. We since ely hank he e iewe s and academic edi o o hei aluable commen s and sugges ions, which imp o ed he quali y o his manusc ip . ADDITIONAL INFORMATION Con lic o in e es The au ho s decla e ha no compe ing in e es s exis . E hical s a emen No e hical s a emen is epo ed. Funding This s udy was inancially suppo ed by Mode n College, Pune‑5 (SEED money 2023‑2024). Au ho con ibu ions Concep ualiza ion: RRK, YSS. In es iga ion: RRK, YSS. Me hodology: RRK, YSS. Resou ces: YSS. Supe ision: YSS, Visualiza ion: RRK. W i ing – o iginal d a : RRK, YSS. W i ing – e iew and edi ing: RRK, YSS. Au ho ORCID iDs Renuka R. Khai na h ps://o cid.o g/0000‑0003‑2283‑4922 Yugandha S. Shinde h ps://o cid.o g/0000‑0001‑6248‑3712 Figu e 4. Dis ibu ion map o T. al onsi Nagle e al., 2014. Check Lis 21 (5) · h ps://doi.o g/10.15560/21.5.884 Khai na and Shinde · Fi s eco d o Tanycyp is al onsi om India 889 Da a a ailabili y All da a ha suppo he indings o his s udy a e a ailable in he main ex . REFERENCES B oodbakke NW, Danielopol DL (1982) The chae o axy o Cyp idacea (C us acea, Os acoda) limbs: p oposals o a desc ip i e model. Bijd agen o de Die kunde 52 (2): 103–120. h ps://doi.o g/10.1163/26660644‑05202003 Ha shey DK, S i as a a AK (1983) Some new eco ds o eshwa e Os acoda (C us acea: En omos aca) om India. Cu en Sci‑ ence 52 (15): 741–772. 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