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Snap Shut Slide Imaging Chamber V1

Kurtz, Ryan

Abstract

The Snap Shut Slide Imaging Chamber V1 is a 3D-printable, reusable chamber designed for stable mounting and live imaging of zebrafish (Danio rerio) embryos under a microscope. The chamber has the same outer dimensions as a standard glass slide and can be viewed from either side, allowing consistent sample positioning during optical imaging. The design was inspired by the viewing chamber constructed from standard glass cover slips described in The Zebrafish Book (Westerfield, 2000). The 3D-printed housing secures the viewing chamber with a snap-fit mechanism, enabling rapid sample preparation, compatibility with multiple microscope types and sample orientations, improved reproducibility, and support for open-hardware practices in developmental biology research.

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Ti le: Snap Shu Slide Imaging Chambe V1 Au ho /Designe : Ryan Ku z, Amhe s College Da e: Sep embe 2025 This 3D model is eleased unde he C ea i e Commons A ibu ion–NonComme cial 4.0 In e na ional License (CC BY-NC 4.0). Licensed unde CC BY-NC 4.0 (h ps://c ea i ecommons.o g/licenses/by-nc/4.0/). You a e ee o: - Sha e — copy and edis ibu e he ma e ial in any medium o o ma - Adap — emix, ans o m, and build upon he ma e ial Unde he ollowing e ms: - A ibu ion — You mus gi e app op ia e c edi , p o ide a link o he license, and indica e i changes we e made. - NonComme cial — You may no use he ma e ial o comme cial pu poses. Requi ed a ibu ion: “This design, Snap Shu Slide Imaging Chambe V1, was c ea ed by Ryan Ku z (2025) and is used unde he CC BY-NC 4.0 license.” I used in a publica ion, please ci e as: Ku z, R. (2025, Oc obe ). Snap Shu Slide Imaging Chambe V1 [3D model]. Amhe s College. Zenodo. h ps://doi.o g/10.5281/zenodo.17244895 No wa an ies a e p o ided. Use a you own isk. Happy imaging. Inspi a ion o his wo k came om: B ad o d, Y.M., Van Slyke, C.E., Ruzicka, L., Singe , A., Eagle, A., Fashena, D., Howe, D.G., F aze , K., Ma in, R., Paddock, H., Pich, C., Ramachand an, S., Wes e ield, M. (2022) Zeb a ish In o ma ion Ne wo k, he knowledgebase o Danio e io esea ch. Gene ics. 220(4). PMID: 35166825 PMCID: PMC8982015 DOI: 10.1093/gene ics/iyac016