249
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
ON THE STUDY OF THE FLAVONOIDS OF LADIGINIA BUCHARICA LIPSKIYI
Komilo Kh.M.
Ik amo a M.Sh.
Mukhi dino a M.K.
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
h ps://doi.o g/10.5281/zenodo.17333616
Backg ound. Wi hin he lo a o Uzbekis an, many wild-g owing plan s emain insu icien ly
and une enly s udied. Conduc ing in-dep h pha macognos ic and pha macological in es iga ions and
in oducing he esul s in o medical p ac ice a e impo an asks. One such plan is he Bukha a
Ladiginia. This species occu s in Cen al Asia (sou he n Pami –Alay) and in Uzbekis an’s
Kashkada ya Region (Kaypan og‘lik a ea). Ea lie , scien is s o he Ins i u e o he Chemis y o Plan
Subs ances, Academy o Sciences o Uzbekis an, in he Glycosides Labo a o y (M. Pa hullae a, N.K.
Abubaki o ), s udied i e pene saponins om he unde g ound pa s o his plan . Howe e , he
bioac i e compounds o he ae ial pa s ( la onoids) ha e no been in es iga ed.
Objec i e. Ladiginia bucha ica belongs o he genus Ladiginia o he amily Apiaceae (cele y
amily), which in Uzbekis an comp ises 73 gene a and 203 species. I is a pe ennial plan 80–150 cm
all. The s udy ocused on he ae ial pa s (s ems, lea es, and lowe s), collec ed du ing lowe ing and
d ied in he shade. The d ied, c ushed aw ma e ial was examined o la onoids.
P elimina y pape ch oma og aphy was ca ied ou a e hea ing he ma e ial wi h 95% e hanol
in a wa e ba h and il e ing. The concen a e was de eloped in he sys em n-bu anol–ace ic acid–
wa e (5:1:4). A e sp aying wi h 3% e hanolic AlCl₃ and d ying a 105 °C, spo s cha ac e is ic o
la onoids we e obse ed unde a UV lamp a R 0.46 and 0.94.
Ma e ials and Me hods. To elucida e he chemical s uc u es o he la onoids, 1.0 kg o d ied,
powde ed aw ma e ial was ex ac ed h ee imes a oom empe a u e wi h 95% e hanol. The
concen a ed ex ac was dilu ed wi h wa e (1:2) and de a ed/dechlo ophyllized by i e successi e
ex ac ions wi h 100 mL po ions o ex ac ion-g ade pe oleum e he , emo ing ballas subs ances
(chlo ophyll). The pu i ied aqueous phase was hen ex ac ed h ee imes wi h 100 mL po ions o n-
bu anol. The combined bu anolic ac ion was e apo a ed in acuo o yield 100 g o d y ex ac .
A cap on (nylon) powde –packed ch oma og aphy column was loaded wi h 35.0 g o his
ex ac . The column was elu ed i s wi h wa e and hen wi h 5%, 10%, and 20% aqueous e hanol.
F ac ions (150 mL each) we e collec ed. The p esence o la onoids in ac ions was moni o ed by
spo ing on il e pape , sp aying wi h 3% e hanolic AlCl₃, hea ing, and examining unde UV ligh .
Resul s. F ac ions displaying la onoid-cha ac e is ic spo s we e e apo a ed in a wa e -ba h
unde educed p essu e; some we e u he pu i ied by ech oma og aphy. Two compounds we e
isola ed:
• Subs ance A: m.p. 278–279 °C; o mula C₁₅H₁₀O₆; M⁺ 286; R 0.94 in n-bu anol–ace ic acid–
wa e (5:1:4). Iden i ica ion was pe o med using he e e ence compound kaemp e ol and by
compa ing mass and UV spec a.
• Subs ance B: m.p. 316–317 °C; o mula C₁₅H₁₀O₇; M⁺ 302; R 0.46. Iden i ica ion was ca ied
ou wi h he e e ence compound que ce in, also by compa ison o mass and UV spec a.
Kaemp e ol and que ce in we e hus isola ed om Ladiginia bucha ica Lipskiyi and iden i ied
o he i s ime.
250
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusion. The ae ial pa s o his wild-g owing plan widesp ead in Uzbekis an con ain he
la onoids kaemp e ol and que ce in. Fu he wo k will con inue on comp ehensi e in es iga ion o
i s bioac i e cons i uen s and on e alua ing hei biological ac i i ies.
