263
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
PHYTOCHEMICAL STUDY OF COMMON BASIL LEAVES
M.I.Xolma o a
D.K.Pula o a
F.F.U mano a
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17334067
Pu pose: his wo k is aimed a s udying he main ac i e ing edien s o basil lea es, which ha e
an i-in lamma o y, chole e ic, an isep ic e ec s.
Ma e ials and me hods o esea ch: he objec o he s udy was he lea es o basil ulga is,
collec ed du ing he lowe ing pe iod in he e i o y o he Bos anlyk dis ic o he Tashken egion.
A p elimina y examina ion o he lea es o he con en o he main g oups o biologically
ac i e subs ances was ca ied ou using well-known quali a i e eac ions and ch oma og aphic
analysis me hods.
To con i m he esul s ob ained and iden i y he de ec ed subs ances, a compa a i e
ch oma og aphic s udy o he ex ac s was ca ied ou , whe e hese subs ances we e de ec ed in he
p esence o eliable "wi ness" samples.
The de e mina ion o la onoids was ca ied ou by ch oma og aphy o aqueous ex ac ion o
he collec ion on pape (Ge man, b and FN-3 Mi elschnell lau end) in a bu anol-ace ic acid-wa e
sol en sys em (4:1:5) using a 1% alcohol solu ion o aluminum chlo ide as a de elope .
Asco bic acid was also de e mined by ch oma og aphy on pape in a sol en sys em o e hyl
ace a e - ace ic acid (80:20); 2,6-dichlo ophenolindophenola e o sodium was used as a de elope .
The essen ial oil was isola ed by hyd odis illa ion
To de ec phenolic acids, alcohol ex ac ion was ch oma og aphed in a bu anol-ace ic acid-
wa e sol en sys em (3:2:95, 4:1:5), 15% and 2% solu ions o ace ic acid, isop opyl alcohol-
ammonia-wa e (8:1:1). UV ligh (be o e and a e ammonia ea men ), a 1% alcoholic solu ion o
e ic chlo ide, diazo ized sul anilic acid, and diazo ized 4-ni oaniline we e used as de elope s.
The p esence o annins was de e mined by quali a i e eac ions wi h 1% gela in solu ion and
3% solu ions o e ic chlo ide and e ic ammonium alum. The polysaccha ide con en was
de e mined by g a ime ic me hod and e hanol p ecipi a ion. The monosaccha ide composi ion o
polysaccha ides a e hyd olysis was de e mined ch oma og aphically simul aneously wi h known
samples on FN-3 pape in he bu anol-pi pdine-wa e (6:4:3) sys em o neu al suga s and e hyl
ace a e-ace ic acid- o mic acid (18:3:4) o acidic suga s. Suga s we e de ec ed wi h an acidic solu ion
o aniline ph hala e and hea ea men .
Resul s: as a esul o ch oma og aphic analysis, a leas ou subs ances o a la onoid na u e
wi h R 0.45; 0.48; 0.55; 0.78 we e ound in he s udied collec ion, iden i ied by compa ison wi h
eliable samples as u in, cina oside, hype oside, que ce in; asco bic acid (0.036%) was iden i ied
om among he o ganic acids. I has also been es ablished ha he phenolic acid composi ion o he
s udied collec ion is ep esen ed by a leas wo subs ances, which, acco ding o he R alue and he
esul s o quali a i e eac ions, a e en a i ely iden i ied as ca eic and chlo ogenic acids. I is shown
ha he monosaccha ide composi ion o he polysaccha ides o he collec ion is ep esen ed by D-
galac ose, L-a abinose, D-glucose and D-xylose, and annins a e among he hyd olyzable.
Conclusions: he esul s ob ained will be used o chemical cha ac e iza ion and
s anda diza ion o basil aw ma e ials.
