267
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
RAMAN SPECTROSCOPIC IDENTIFICATION OF SULFAMETHOXAZOLE IN
PHARMACEUTICAL DOSAGE FORMS
N.B. Saidka imo a
A.N. Yunuskhodjaye
Tashken Pha maceu ical Ins i u e, Tashken ci y, Republic o Uzbekis an
e-mail: [email p o ec ed]
h ps://doi.o g/10.5281/zenodo.17334129
In oduc ion. Sul onamides a e a g oup o compounds wi h an ibac e ial ac i i y ha ha e
been widely used in medicine since he ea ly 20 h cen u y. These subs ances exe a bac e ios a ic
e ec by inhibi ing he g ow h and ep oduc ion o mic obial cells. The mechanism o ac ion o
sul onamides is based on he dis up ion o he biosyn hesis o essen ial me aboli es in bac e ia, such
as olic acid and dihyd o ola e.
Gi en such an impo an biochemical mechanism, he analysis o sul onamides, he s udy o
hei s uc u e, and hei accu a e iden i ica ion a e o g ea signi icance in mode n esea ch. Fo his
pu pose, highly sensi i e and e icien spec oscopic echniques a e equi ed. In pa icula , Raman
spec oscopy has been ecognized as a eliable and ele an me hod o he iden i ica ion o
compounds like sul onamides, which exhibi cha ac e is ic ib a ional modes in hei molecula
s uc u e.
Ma e ials and me hods. The analyses on Raman spec ome e we e pe o med on a model R-
532 wi h a spec al ange om 100 o 6000 cm-1, he spec al esolu ion o 5-8 cm-1, lase wa eleng h
532 nm, lase powe 50 mW, linea CCD a ay, pixel numbe 3648, ocal leng h 75 mm, en ance
ape u e 20-30 mic ons, holog aphic di ac ion g a ing 1800 lines/mm.
The aim o he s udy is o in es iga e he Raman spec a o sul ame hoxazole-con aining able
and suspension dosage o ms, and o e alua e he po en ial o Raman spec oscopy o he
iden i ica ion o he ac i e pha maceu ical ing edien wi hin hese o mula ions.
In he Raman spec um o sul ame hoxazole, one o he sul onamide d ugs con aining a
he e oa oma ic ing, all cha ac e is ic bands co esponding o he sul onyl (SO2), a oma ic ing,
isoxazole, and amino (–NH2) g oups a e p esen . The main peaks include cha ac e is ic sul onyl
ib a ions obse ed a ≈1090, 1142, and 1154 cm-1, which co espond o νsym(SO2), νas(SO2), and
hei combina ions. The ing-b ea hing mode o he pa a-subs i u ed a oma ic ing is clea ly exp essed
a ≈1003–1015 cm-1. In he egion o 1500–1650 cm-1, wo dis inc peaks a e obse ed a ≈1589 and
≈1634 cm-1, which co espond o a oma ic ν(C=C) ib a ions and de o ma ion modes o he –NH2
g oup, while N–H s e ching ib a ions a e eco ded in he ≈3300–3470 cm-1 ange. The skele al
ib a ion o he isoxazole ing is loca ed a 923 cm-1 (Figu e 1).
Figu e 1. Raman spec um o sul ame hoxazole subs ance
268
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
In he cou se o he s udy, he Raman spec a o sul ame hoxazole-con aining suspension and
able dosage o ms we e also eco ded, and he p esence o hei cha ac e is ic sca e ing peaks we e
obse ed.
Conclusion. The esul s o he s udy demons a ed ha cha ac e is ic Raman sca e ing bands
o sul ame hoxazole we e clea ly obse ed in bo h suspension and able dosage o ms. This con i ms
ha Raman spec oscopy can be ega ded as a eliable me hod o he iden i ica ion o
sul ame hoxazole in pha maceu ical o mula ions.
