372
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
DETERMINATION OF ANTIOXIDANT PROPERTIES OF FACTORY-GROWN
CHICKEN EGGSHELLS
Fayziye X.O.
Uz. Res. Mili a y o ice o he Minis y o De ense, esea che o chemical sciences.
[email p o ec ed]
Islomo Akmal Xush aqo ich
Doc o o Chemical Sciences, Ins i u e o Bioo ganic Chemis y, Academy o Sciences o he
Republic o Uzbekis an.
Mus a akulo M.A.
Senio Resea che , Ins i u e o Biophysics and Biochemis y unde he M. Ulugbek Na ional
Uni e si y o Uzbekis an., PhD
h ps://doi.o g/10.5281/zenodo.17337909
Rele ance o he s udy: Today, bone diseases a e inc easing due o calcium de iciency, and o
p e en and elimina e his condi ion, eggshells a e a na u al sou ce ich in calcium. They a e no only
a sou ce o calcium, bu also play an impo an ole in he abso p ion o D3, which is ob ained wi h
sola ene gy, in o he body.
Pu pose o he s udy: To de e mine he an ioxidan p ope ies o ac o y-g own chicken
eggshells.
Resea ch me hods: Washed and cleaned eggshells, d ied a oom empe a u e, we e g ound
in o powde . The an ioxidan ac i i y o he powde ed sample was de e mined by he me hod o in
i o au ooxida ion o ad enaline.
Resea ch esul s: The an ioxidan ac i i y o eggshells was de e mined by he inhibi ion o he
in i o au ooxida ion eac ion o ad enaline.
Fo his pu pose, 2.0 ml o 0.2 M sodium ca bona e (Na2CO3-NaHCO3) bu e wi h pH=10.65,
56 μl o 0.18% ad enaline (epineph ine) hyd ochlo ide solu ion we e aken, 30 μl o an ioxidan
p epa a ion we e added and, wi h apid mixing, he sample was examined in a 10 mm cu e e a a
wa eleng h o 347 nm o 30 seconds o 10 minu es on a Ca y 60 UV-Vis Agile Technologies
spec opho ome e . Fo his pu pose, 2.0 ml o 0.2 M sodium ca bona e (Na2CO3-NaHCO3) bu e
wi h pH=10.65, 56 μl o 0.18% ad enaline (epineph ine) hyd ochlo ide solu ion we e aken, 30 μl o
an ioxidan p epa a ion we e added and, wi h apid mixing, he sample was examined in a 10 mm
cu e e a a wa eleng h o 347 nm o 30 seconds o 10 minu es on a Ca y 60 UV-Vis Agile
Technologies spec opho ome e . The amoun o he es subs ance (concen a ion o he ex ac in 1
ml is 1 mg) is used as a s anda d. As a con ol sample, 0.2 M 2.0 ml bu e , 0.18% 56 μl (5.46 mM)
ad enaline is used.
The an ioxidan ac i i y was exp essed as a pe cen age o he inhibi ion o ad enaline
au ooxida ion and was calcula ed by he ollowing o mula.
АА%=D1−D2 x100
𝐷1
Op ical densi y o ad enaline hyd ochlo ide solu ion added o bu e D1;
Op ical densi y o he ex ac unde s udy and ad enaline hyd ochlo ide added o bu e D2.
S a is ics we e es ed using he T-s uden es and O ijen 6.1 US so wa e.
The an ioxidan capaci y o he d ugs is de e mined o he in i o o m.
Sample
Composi ion
Solubili y
In i o; mkg/ml
Home eggshell ex ac
Eggshell ex ac
30% alcohol and wa e
30
373
Volume 5, Issue 10: Special Issue
(EJAR)
ISSN: 2181-2020
MPHAPP
THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL
CONFERENCE “MODERN PHARMACEUTICS: ACTUAL
PROBLEMS AND PROSPECTS”
TASHKENT, OCTOBER 17, 2025
in-academy.uz
Conclusions: The an ioxidan ac i i y o he p epa a ions was de e mined by he me hod o in
i o au oxida ion o ad enaline. The an ioxidan ac i i y o he s udied p epa a ions was assessed by
chemical es s. The an ioxidan ac i i y o he p epa a ions was de e mined by he inhibi ion o he in
i o au oxida ion eac ion o ad enaline and he p e en ion o he o ma ion o ee oxygen species.
The p epa a ions we e compa ed wi h he s anda d an ioxidan s que ce in and gliclazide. The
ob ained p epa a ions show he p esence o an ioxidan p ope ies.
Que ce in
Japanese
Embassy
30% alcohol and wa e
30
Gliklazide
-
wa e
30
