ʼPEROVSKIA KUDRJASCHEVII – A PROMISING SOURCE OF BIOLOGICALLY ACTIVE TERPENOIDS

376 Volume 5, Issue 10: Special Issue (EJAR) ISSN: 2181-2020 MPHAPP THE 6TH INTERNATIONAL SCIENTIFIC AND PRACTICAL CONFERENCE “MODERN PHARMACEUTICS: ACTUAL PROBLEMS AND PROSPECTS” TASHKENT, OCTOBER 17, 2025 in-academy.uz ʼPEROVSKIA KUDRJASCHEVII – A PROMISING SOURCE OF BIOLOGICALLY ACTIVE TERPENOIDS Ikromova G. M.1 BotirovE. Kh.2 1Namangan State University, Namangan, Republic of Uzbekistan, e-mail: [email protected], phone (+998) 99 398 22 98 2Institute of Plant Chemistry named after academician S.Yu. Yunusov, AS RUz, Tashkent https://doi.org/10.5281/zenodo.17339069 Relevance: Perovskia kudrjaschevii Gorschk. & Pjataeva (family Lamiacaeae) is a medicinal, essential oil, dye and honey plant. It is found in Uzbekistan, Tajikistan and Kyrgyzstan. The chemical composition of the components of Perovskia kudrjaschevii has not been previously studied. Purpose of the study. Isolation and study of biologically active components of the aboveground part and roots of Perovskia kudrjaschevii, collected in the Bostandyk district of the Tashkent region. Materials and methods. Crushed air-dried plant materials were extracted five times at room temperature with methanol. The combined extract was evaporated in vacuo, diluted with water and subjected to liquid-liquid extraction with benzene, chloroform, ethyl acetate and n-butanol. Results. Two phenolic carboxylic acids, one diterpenoid and two phytosterols were isolated from different fractions of the methanol extract of the aerial parts by column chromatography on silica gel and Sephadex LH-20. Based on the study of UV, 1H and 13C NMR spectra the isolated substances were identified with caffeic and rosmarinic acids, ferruginol, β-sitosterol and stigmasterol. Fourteen individual compounds were isolated from the methanol extract of the roots by column chromatography on silica gel and Sephadex LH-20. Based on the study of UV, 1H and 13C NMR spectra the isolated substances were identified with the bis-nor-diterpenoid przewalskin, norditepenoids cryptotanshinone and tanshinone IIA, ditepenoids 7α-acetoxyroileanone and sujiol, triterpenoids erythrodiol 3β-acetate, olean-12-ene-3β-O-acetyl-28-al, oleanolic acid and oleanolic acid 3-acetate. In addition to the above compounds, β-sitosterol, stigmasterol, caffeic and rosmarinic acids were isolated and identified from the roots. The spatial structure of 7α-acetoxyroileanone, erythrodiol 3β-acetate and olean-12-ene-3β-O-acetyl-28-al was established by X-ray diffraction analysis (XRD). The XRD materials of the above compounds were deposited at the Cambridge Crystallographic Data Centre. All the isolated compounds have a diverse spectrum of pharmacological properties. Cryptotanshinone is characterized by anticancer, anti-inflammatory, immunoregulatory, neuroprotective and antifibrotic activity. Cryptotanshinone exhibits a pronounced anti-inflammatory effect in the experiment, suppressing both the exudative and proliferative phases of inflammation. In terms of the severity of its action, cryptotanshinone has shown certain advantages over diclofenac. Tanshinone IIA dilates coronary arteries and increases coronary blood flow due to the activation of potassium channels, has a cardioprotective and anti-inflammatory effect. Conclusions. It has been shown that the Perovskia kudrjaschevii plant is a rich source of biologically active phenolic carboxylic acids, diterpenoids, triterpenoids and can be used to create effective drugs and dietary supplements.