Supplementary material 1 from: Lapeva-Gjonova A, Pramatarova M, Borowiec L, Gjonov I, Kostova R, Bekchiev R, Borissov S (2025) DNA barcoding of Messor ants of Bulgaria with insights into their taxonomic diversity. Biodiversity Data Journal 13: e168586. https://doi.org/10.3897/BDJ.13.e168586

Supplemen a y ma e ials. COI gene ic dis ance (%) wi hin s udied Messo species
species
n
min
max
Messo a anasso ii
5
0
1.41
Messo sp. 1 (nea M. wasmanni)
4
0
0.61
Messo wasmanni
13
0
0.81
Messo oe zeni
3
0
0.15
Messo s uc o
17
0
5.07
Messo mca hu i
2
0
0.00
Messo hellenius
24
0
0.99
Messo pon icus
4
0
1.38
Messo ibe icus
7
0
0.46
COI gene ic dis ance (%) be ween Messo species unde he Kimu a 2-pa ame e model. Values in b acke s ep esen he mean wi hin species dis ance. NA/n.a.: no
a ailable.
N species 1 2 3 4 5 6 7 8 9 10 11 12
1.
M. c e icus NA (n.a)
2.
M. a anasso ii 1.92 NA (0.74)
3.
Messo sp. 1 (nea M.
wasmanni)
5.98 6.28 NA (0.31)
4.
M. wasmanni 5.80 9.58 5.00 NA (0.18)
5.
M. oe zeni 9.05 9.40 10.71 10.24 NA (0.10)
6.
M. s uc o 8.99 9.18 10.20 10.18 9.22 NA (2.57)
7.
M. mca hu i 9.73 9.98 10.14 11.55 8.89 8.49 NA (0)
8.
M. hellenius 9.50 9.66 10.12 10.88 9.13 8.48 6.47 NA (0.35)
9.
M. pon icus 9.56 9.64 10.67 11.28 9.40 8.57 7.01 1.63 NA (0.83)
10.
M. ibe icus 9.26 9.48 10.29 10.72 8.93 8.40 6.73 3.20 3.30 NA (0.20)
11.
Messo c . s uc o 7.00 7.61 8.83 9.50 7.94 7.79 7.37 6.19 6.39 6.50 NA (n.a)
12.
Messo sp. 2 (nea M.
oe zeni)
10.61 10.64 11.62 11.73 5.72 9.81 9.11 8.88 8.84 9.21 7.38 NA (n.a)
#Resul o GMYC species delimi a ion
me hod: single; likelihood o null model: 636.1001; maximum; likelihood o GMYC model: 641.692; likelihood a io: 11.1838
esul o LR es : 0.003727933**; numbe o ML clus e s: 13; con idence in e al: 3-23; numbe o ML en i ies: 21;
con idence in e al: 3-42; h eshold ime: -0.002905204
M. c e icus
M. a anasso ii
Messo sp. 1
M. wasmanni
Мesso c . s uc o
Messo sp. 2
M. oe zeni
M. s uc o
M. mca hu i
M. hellenius
M. pon icus
M. ibe icus
Messo sp.|BGANT033-23
Messo helleniusBGANT016-23
Messo helleniusBGANT029-23
Messo helleniusBGANT039-23
Messo helleniusBGANT040-23
Messo helleniusBGANT050-23
Messo helleniusBGANT052-23
Messo helleniusBGANT055-23
Messo helleniusBGANT057-23
Messo helleniusBGANT066-23
Messo c . hellenius|BGANT072-23
Messo helleniusBGANT058-23
Messo helleniusBGANT071-23
Messo helleniusBGANT089-23
Messo helleniusBGANT090-23
Messo helleniusBGANT051-23
Messo helleniusBGANT054-23
Messo helleniusBGANT069-23
Messo helleniusBGANT035-23
Messo helleniusBGANT053-23
Messo helleniusBGANT056-23
Messo helleniusBGANT061-23
Messo helleniusBGANT064-23
Messo hellenius|BGANT068-23
Messo pon icus|BGANT062-23
Messo pon icus|BGANT063-23
Messo pon icus|DQ074353
Messo pon icus|BGANT031-23
Messo ibe icus|BGANT021-23
Messo ibe icus|BGANT023-23
Messo ibe icus|BGANT025-23
Messo ibe icus|BGANT059-23
Messo ibe icus|BGANT077-23
Messo ibe icus|BGANT094-23
Messo ibe icus|KT184511-23
Messo mca hu i|BGANT015-23
Messo mca hu i|BGANT091-23
Messo mca hu i|KT184569-23
Messo s uc o |BGANT036-23
Messo s uc o |BGANT045-23
Messo s uc o |BGANT046-23
Messo s uc o |BGANT027-23
Messo s uc o |BGANT024-23
Messo s uc o |BGANT038-23
Messo s uc o |BGANT022-23
Messo s uc o |BGANT026-23
Messo s uc o |BGANT034-23
Messo s uc o |BGANT042-23
Messo s uc o |BGANT044-23
Messo s uc o |BGANT048-23
Messo s uc o |BGANT049-23
Messo s uc o |BGANT041-23
Messo s uc o |BGANT047-23
Messo s uc o |BGANT067-23
Messo s uc o |BGANT037-23
Messo s uc o |KT184551
Messo oe zeni|BGANT013-23
Messo oe zeni|BGANT014-23
Messo oe zeni|BGANT073-23
Messo sp. 2|BGANT009-23
Messo c . s uc o |BGANT032-23
Messo wasmanni|BGANT007-23
Messo wasmanni|BGANT008-23
Messo wasmanni|BGANT011-23
Messo wasmanni|BGANT074-23
Messo wasmanni|BGANT079-23
Messo wasmanni|BGANT060-23
Messo wasmanni|BGANT081-23
Messo wasmanni|BGANT082-23
Messo wasmanni|BGANT083-23
Messo wasmanni|BGANT084-23
Messo wasmanni|BGANT085-23
Messo wasmanni|BGANT086-23
Messo wasmanni|BGANT088-23
Messo sp. 1|BGANT003-23
Messo sp. 1|BGANT004-23
Messo sp. 1|BGANT010-23
Messo sp. 1|BGANT070-23
Messo a anasso ii|BGANT002-23
Messo a anasso ii|BGANT005-23
Messo a anasso ii|BGANT012-23
Messo a anasso ii|BGANT001-23
Messo a anasso ii|BGANT078-23
Messo c e icus|BGANT087-23
Aphaenogas e es ae|BGANT095-23
T ee scale: 0.01
97.0
1.00
1.00
0.53
1.00
0.98
1.00
0.97
1.00
1.00
1.00
0.97
1.00
1.00
0.94
0.79
1.00
59.0
0.95
0.97
0.93
0.68
1.00
0.55
0.59
1.00
0.80
1.00
1.00
0.71
1.00
0.95
0.95
0.98
1.00
1.00
0.66
1.00
0.99
0.69
Phylogene ic ee based on Bayesian in e ence (BI) analysis o COI gene agmen .
Nodal suppo is gi en as pos e io p obabili y alues. We conside ed signi ican ly high nodal suppo o
pos e io p obabili ies (PP) > 0.95, and mode a ely good suppo o PP > 0.90–0.94.