Design and ab ica ion o nanos uc u ed disposable
elec ochemical bio-sensing chips o he quick moni o ing o
Alzheime 's disease bioma ke s
Backg ound
Figu e 2: (A-B) Nanoma e ial Composi e selec ion. (A) Cyclic ol amme ic (CV) cha ac e iza ion o
p in ed elec odes modi ied wi h di e en nanoma e ial composi es. (B) Elec ochemical impedance
spec oscopic (EIS) cha ac e iza ions o p in ed elec odes modi ied wi h di e en nanoma e ial composi es.
Conclusion and Fu u e Di ec ion
Nehal I. Ghoneim 1, 2 , Ahmed Abdella i 1* and Rabeay Y. A. Hassan 2*
1. Bio echnology g adua e p og am, and Depa men o biology, School o Sciences and Enginee ing, he Ame ican Uni e si y in Cai o, Cai o 11835, Egyp .
2. Biosenso s Resea ch Cen e , Uni e si y o Science and Technology (UST), Zewail Ci y o Science and Technology, Giza 12578, Egyp .
Figu e 4: Tes ing he immune-sensing imes e ec on he cap u ing e iciency o he an igen.
Ca ching Alzheime ’s Be o e I Ca ches
Memo ies: Ea ly Signals, Las ing Memo ies wi h
Biosensing.
Neu odegene a i e diseases (NDs) like Alzheime ’s disease (AD),
Pa kinson’s disease, and ALS in ol e p og essi e neu onal
degene a ion, wi h AD being he mos p e alen demen ia ype, ma ked
by memo y loss and a ec ing ~50 million people globally. I s pa hology
ea u es amyloid-be a plaques and au p o ein angles, causing neu onal
dea h. Cu en diagnosis elies on in asi e, cos ly me hods like b ain
imaging and CSF analysis, hinde ing ea ly de ec ion. Elec ochemical
nano-biosenso s o e a ans o ma i e solu ion, enabling apid, non-
in asi e, and sensi i e de ec ion o bioma ke s such as amyloid-be a
oligome s and phospho yla ed au p o eins. These biosenso s le e age
nanocomposi es and molecula ly imp in ed polyme s o enhance
speci ici y and po abili y o poin -o -ca e use. Ou s udy ocuses on
de eloping an immunosenso pla o m using sc een-p in ed elec odes o
de ec plasma AD bioma ke s, aiming o imp o e ea ly diagnosis and
pa ien ou comes globally.
S eps o ab ica ion o he immune-sensing sys em
-
1. Abbasi, H. Y., Teh ani, Z., De adoss, A., Ali, M. M., Mo adi-Bachille , S., Albani, D., & Guy, O. J. (2021). G aphene based
elec ochemical immunosenso o he ul a-sensi i e label ee de ec ion o Alzheime 's be a amyloid pep ides Aβ (1–42). Nanoscale
Ad ances,3(8), 2295-2304.
2. Sha ma, A., Angnes, L., Sa a ahmady, N., Negahda y, M., & Heli, H. (2023). Elec ochemical Immunosenso s De eloped o Amyloid-
Be a and Tau P o eins, Leading Bioma ke s o Alzheime ’s Disease. Biosenso s,13(7), 742.
Re e ences
This wo k is suppo ed by he AUC esea ch suppo g an ,
and he join EPFL-UM6P ini ia i e “Excellence in A ica”, he
100 PhDs o A ica p og amme.
Acknowledgmen
Me hodology
AB
Figu e 3: (A) EIS Cha ac e iza ion o p in ed elec odes wi h di e en c osslinke s ( elec odeposi ed POPD s
4-ATP, (B) EIS moni o ing o he immunosenso manu ac u ing s eps including he d op-cas ing o
nanocomposi es on o he p in ed elec ode su ace, o ma ion o a sel -assembled monolaye o he P-OPD,
conjuga ion o he an ibody, blocking he non-speci ic binding wi h he BSA.
Figu e 5: Nyquis plo s gene a ed by he Amyloid be a-42-immunosenso agains di e en concen a ions o
Amyloid be a-42. Calib a ion cu e o he Amyloid be a-42. ΔRc alues a e ex ac ed om (A) h ough a
modeled equi alen ci cui .
Figu e 6: Selec i i y es ing o he Amyloid be a-42 -immunosenso owa ds non- a ge ing common bioma ke s.
Ten- old inc ease in he concen a ion o each o he non- a ge ing molecules was used o his expe imen .
The MWCNT-Ru nanocomposi e signi ican ly enhanced he immunosenso ’s sensi i i y,
enabling apid, low-concen a ion de ec ion o amyloid-be a-42 (LOD: 0.002 ng/mL) wi h
high selec i i y agains non- a ge bioma ke s. This cos -e ec i e, po able pla o m shows
p omise o ea lyAlzheime ’s diagnosis in poin -o -ca e se ings.
Figu e 1: G aphical Abs ac o he wo k. SEM images o (A) Elec odeposi ed P-OPD on he su ace o he
modi ied elec ode wi h MWCNT-Ru composi e. (B) Ru henium (Ru) nanopa icles.
Con ac : Nehalgho[email p o ec ed]
