Acid and enzymatic fractionation of olive stones for ethanol production using Pachysolen tannophilus

processes Article Acid and Enzymatic Fractionation of Olive Stones for Ethanol Production Using Pachysolen tannophilus Manuel Cuevas 1,2,* , Marwa Saleh 1, Juan F. García-Martín2,3,* and Sebastián Sánchez 1,2 1Department of Chemical, Environmental and Materials Engineering, University of Jaén, Campus ‘Las Lagunillas’, 23071 Jaén, Spain; [email protected] (M.S.); [email protected] (S.S.) 2Center for Advanced Studies in Olive Grove and Olive Oils, Science and Technology Park GEOLIT, 23620 Mengibar, Spain 3Departamento de Ingeniería Química, Facultad de Química, Universidad de Sevilla, C/Profesor García González, 1, 41012 Seville, Spain *Correspondence: [email protected] (M.C.); [email protected] (J.F.G.-M.) Received: 29 December 2019; Accepted: 31 January 2020; Published: 6 February 2020   Abstract: Olive stones are an abundant lignocellulose material in the countries of the Mediterranean basin that could be transformed to bioethanol by biochemical pathways. In this work, olive stones were subjected to fractionation by means of a high-temperature dilute-acid pretreatment followed by enzymatic hydrolysis of the pretreated solids. The hydrolysates obtained in these steps were separately subjected to fermentation with the yeast Pachysolen tannophilus ATCC 32691. Response surface methodology with two independent variables (temperature and reaction time) was applied for optimizing D-xylose production from the raw material by dilute acid pretreatment with 0.01 M sulfuric acid. The highest D-xylose yield in the liquid fraction was obtained in the pretreatment at 201 ◦ C for 5.2 min. The inclusion of a detoxification step of the acid prehydrolysate, by vacuum distillation, allowed the fermentation of the sugars into ethanol and xylitol. The enzymatic hydrolysis of the pretreated solids was solely effective when using high enzyme loadings, thus leading to easily fermentable hydrolysates into ethanol. The mass macroscopic balances of the overall process illustrated that the amount of inoculum used in the fermentation of the acid prehydrolysates strongly affected the ethanol and xylitol yields. Keywords: bioethanol; dilute acid pretreatment; enzymatic hydrolysis; olive stones; Pachysolen tannophilus; response surface methodology 1. Introduction Global warming is a problem that could be mitigated by replacing fossil energy sources by renewable energy sources, such as green biomass. [ 1 ]. In recent decades numerous research papers have addressed the use of lignocellulose materials to obtain ethanol through biochemical routes [ 2 – 4 ], describing bioprocesses that are mainly composed of three major stages: Biomass pretreatment, cellulose hydrolysis, and sugars fermentation. Acid prehydrolysis at high temperature (around 200 ◦ C) is one of the most efficient pretreatments to remove hemicelluloses and extracts present in lignocellulose materials, obtaining a cellulose and lignin-rich pretreated solid [ 5 , 6 ]. This type of pretreatment can be carried out with low concentrations of acid and short reaction times (few minutes), being able to generate liquid prehydrolysates with high concentrations of hemicellulose sugars, provided that suitable operating conditions are used. Thus, for a fixed acid concentration, there will be optimal temperature and reaction time conditions that will lead to complete hydrolysis of the hemicellulose and will maximize the concentration of hemicellulose sugars in the liquid prehydrolysate. The search for these conditions could be approached using response surface methodology as previously described Processes 2020,8, 195; doi:10.3390/pr8020195 www.mdpi.com/journal/processes Processes 2020,8, 195 2 of 14 elsewhere[ 7 , 8 ]. In relation to the type of acid used, sulfuric acid is generally used instead of hydrochloric acid due to its low volatility, lesser equipment corrosion and lower cost per mole of protons [ 9 ], so it is frequently used at this stage of the bioprocess. To improve the biomass fractionation, pretreated solids could be subjected to enzymatic hydrolysis with cellulases, which would allow a selective conversion of cellulose into D-glucose under mild operating conditions (pH 4.8, 50 ◦ C temperature). It is of great importance to assess the effect of enzyme loading in the enzymatic hydrolysis, since a low concentration of the same would lead to low monosaccharide yields, while a high concentration would excessively increase the operating costs. Concerning sugars fermentation, the use of non-traditional yeasts, such as Pachysolen tannophilus, would allow converting both hexoses and pentoses into ethanol [ 10 ], provided that fermentative inhibitors concentrations are cut down. In this sense, the levels of furfural and acetic acid in the acid prehydrolysates could be reduced by vacuum distillation because of the higher vapor pressures of these compounds with respect to those of monosaccharides. A variable of great interest in the fermentative stage is the inoculum concentration since this is responsible, among other factors, for the bioprocess rate. Olive stones are a lignocellulose material that is obtained in great quantities in the olive oil mills [ 11 ]. Therefore, this biomass is a potential source of biofuels in countries with large olive production, such as Spain, Italy, Greece, or Portugal. The biochemical conversion of olive stones into ethanol, using hydrothermal or acid pretreatments, has been partially studied by different authors. Thus, Miranda et al. [ 12 ] applied a hydrothermal pretreatment to olive stones (130 ◦ C, 30 min) achieving a good enzymatic digestibility of the cellulose fraction. However, the further use of liquid prehydrolysates is usually not addressed. The liquid prehydrolysates are rich in D-xylose and xylooligosaccharides as described in other works in which water was used as a hydrolytic agent [ 13 , 14 ]. On the other hand, Romero-Garc í a et al. [ 15 ] applied 2% (wt.) sulfuric acid (130 ◦ C, 60 min) to olive stones, achieving a high production of hemicellulose sugars (mainly D-xylose) that were fermented to ethanol after undergoing detoxification by overliming. Notwithstanding, this work did not address the use of the pretreated solids, thus remaining as waste. Saleh et al. [ 16 ] applied dilute sulfuric acid (0.025 M H 2 SO 4 ) to olive stones (195 ◦ C, 5 min) to hydrolyze the hemicellulose and obtain a D-xylose-rich prehydrolysate, which was subsequently detoxified and fermented. The pretreated solids were subjected to enzymatic hydrolysis, but the obtained hydrolysates were not fermented despite their high sugars content. The present study aims to develop a complete scheme of ethanol production from olive stones by applying two consecutive hydrolytic stages (acidic and enzymatic) followed by the subsequent stage of sugars fermentation. The acid pretreatment was optimized to maximize the recovery of hemicellulose sugars, while the effect of enzyme loading on D-glucose production in the subsequent stage of enzymatic hydrolysis was studied. The non-traditional yeast Pachysolen tannophilus was used for the fermentation of both the acid prehydrolysates and the enzymatic hydrolysates, and the effect of inoculum concentration on the fermentation performance was assessed. The fractionation applied to the biomass was envisaged by determining the mass macroscopic balances at the different stages of the process. In this way, a rapid description of the conversion of olive stones into ethanol and other bioproducts was achieved. 2. Materials and Methods 2.1. Raw Material Olive stones (fragmented endocarps) were supplied by the olive mill S.C.A. San Juan (Ja é n, Spain, UTM coordinates: 37 ◦ 47 0 58.52 00 N, 3 ◦ 47 0 09.42 00 W). Once at laboratory, the raw material was washed and then dried at room temperature for three weeks. Afterwards, the olive stones were screened using a vibratory screen (Restch, Mod. Vibro). The solids used in this research had diameters lower than 3 mm while those of diameter lower than 0.85 mm represented less than 3% of the total sample weight. Finally, the dry solids were stored in sealed plastic bags at room temperature until used. Processes 2020,8, 195 3 of 14 2.2. Dilute Acid Pretreatment Dilute acid hydrolysis was carried out in a 2 dm 3 Parr reactor, Series 4522 (Moline, IL, USA). Fo experiments, this reactor was loaded with 50 g of dry solids and 300 cm 3 of 0.010 M sulfuric-acid solution. The chosen H2SO4concentration was significantly lower than that used in a previous work published by us (0.025 M, [ 16 ]) to reduce the corrosion that the acid causes on the reactor. The mixture was stirred at 250 rpm and quickly heated up to work temperature, which was maintained over the selected reaction time (see Section 2.3). Subsequently, the reactor was cooled to room temperature (in less than 10 min) by circulating cold water through an internal coil. The liquid prehydrolysate was separated from the pretreated solid by vacuum filtration. The latter one was water-washed and dried at room temperature. The water used for washing the pretreated solid was added to the prehydrolysate until reaching a final volume of 2 dm 3 . The two separate phases were stored for later characterization and used in the following stages of the scheme. 2.3. Response Surface Methodology (RSM) Response surface methodology (RSM) was applied to data to optimize the acid pretreatment conditions by multiple regression analysis. To do this, the Modde 7.0 (Umetrics AB, Umeå, Sweden) software was used. The 2 2 central composite circumscribed design (CCCD) with two independent variables (temperature and time) at two different levels, four star (axial) points and three central points (total 11 runs, Table 1) was assayed to find linear, quadratic and interaction effects of the independent variables (operational parameters) on the experimental responses (experimental results). The temperature (180–220 ◦ C) and time (2–8 min) ranges were selected from the results obtained in preliminary experiments (data not shown) with the aim of achieving as D-xylose recovery as possible in the liquid prehydrolysates. The statistical validation was carried out by one-way ANOVA test (95% confidence), and the optimal conditions values were determined from the response surfaces using the SIMPLEX method. Table 1. Operational conditions assayed as dimensional and dimensionless independent variables. Run Temperature (◦C) Time (min) Actual Values (T) Coded Values (X1) Actual Values (t) Coded Values (X2) 1 172 0 5.00 −1.414 2 180 −1 2.00 −1 3 180 1 8.00 −1 4 200 −1.414 0.76 0 5 200 0 5.00 0 6 200 0 5.00 0 7 200 0 5.00 0 8 200 1.414 9.24 0 9 220 −1 2.00 1 10 220 1 8.00 1 11 228 0 5.00 1.414 2.4. Detoxification and Fermentation of Acid Prehydrolysates The acid prehydrolysates were detoxified using a Buchi R-114 rotary evaporator (BÜCHI Labortechnik AG, Flawil, Switzerland) at 40 ◦ C, thus removing inhibitor compounds (acetic acid, furfural, and 5-hydroxymethylfurfural) and achieving a D-xylose concentration close to 15 g/dm3. Fermentations of acid prehydrolysates were carried out using the yeast P. tannophilus ATCC 32,691, supplied by the American Type Culture Collection. The microorganism was stored in a cold room (5–10 ◦ C) in 100-cm 3 test tubes on a sterilized solid culture medium with the following composition: 3 g/dm 3 yeast extract (Becton Dickinson Co), 3 g/dm 3 malt extract (Merck), 5 g/dm 3 peptone from casein (Merck), 10 g/dm 3 D-xylose ( ≥ 99% purity, Panreac), and 20 g/dm 3 agar–agar (Panreac). For pre-inocula, Processes 2020,8, 195 4 of 14 cells were transferred to a sterile medium with the same aforementioned composition and kept in an incubator at 30 ◦ C for 60 h in order to obtain cells at the same growth stage at the beginning of each fermentation. Afterwards, the pre-inocula were transferred to 250-cm 3 Erlenmeyer flasks along with 100 cm3of sterile liquid culture made of 4 g/dm3yeast extract (Becton Dickinson Co), 3.6 g/dm3 peptone from casein (Merck), 3 g/dm 3 (NH 4 ) 2 SO 4 (99% purity, Panreac), 2 g/dm 3 MgSO 4· 7H 2 O (99,5% purity, Carlo Erba), 2 g/dm 3 KH 2 PO 4 (99% purity, Panreac), and 25 g/dm 3 D-xylose ( ≥ 99% purity, Panreac). Cultures were incubated at 30 ◦ C for 24 h in an orbital shaker (150 rpm). Then, yeast cells were recovered by centrifugation at 7000 rpm for 10 min, washed with a dilute NaCl solution, and suspended in the fermentation medium to obtain initial inoculum concentrations of 0.5, 1.0, 2.0, and 4.0 g/dm 3 . These concentrations (x, kg/m 3 ) were calculated from the absorbances of the cultures at 620 nm using a previously obtained absorbance versus dry-weight calibration line [10]. Fermentations were carried out with 30 cm 3 of prehydrolysate inside 100 cm 3 Erlenmeyer flasks. The prehydrolysates were supplemented with 2 g/dm 3 yeast extract (Becton Dickinson Co), 1.8 g/dm 3 peptone from casein (Merck), 1.5 g/dm 3 (NH 4 ) 2 SO 4 (99% purity, Panreac), 1 g/dm 3 MgSO 4· 7H 2 O (99,5% purity, Carlo Erba), and 1 g/dm 3 KH 2 PO 4 (99% purity, Panreac). The resulting cultures were sterilized using a glasswool pre-filter and a 0.2- µ m pore-size cellulose nitrate filter. Temperature (30 ◦ C) and pH (4.5) were chosen according to previous works [ 10 , 17 ] and kept constant over fermentations. The aeration was only supplied by the stirring vortex (microaerobic conditions). The cultures were sampled at fixed intervals to analyze the biomass, D-glucose, D-xylose, acetic acid, ethanol, and xylitol concentrations. Two replicas of each fermentation were performed. 2.5. Enzymatic Hydrolysis The washed and dried water-insoluble solids obtained in the dilute acid hydrolysis of olive stones were submitted to enzymatic hydrolysis in order to obtain D-glucose from the cellulose. To do this, 3 g of dry solids were suspended in 30 cm 3 of 0.05 M citrate buffer solution (pH 4.8) inside 125 cm 3 Erlenmeyer flasks. Enzymatic hydrolyses were carried out at 50 ◦ C for 72 h on an orbital shaker (150 rpm). A commercial preparation of Trichoderma reesei cellulases (Celluclast 1.5L, Novo Nordisk Bioindustrial, Madrid, Spain) was used throughout this research. Enzyme loadings of 10, 20, 40 and 60 FPU per g dried solid were added to the Erlenmeyer flasks. 1-cm 3 samples were withdrawn from the reaction media at 4, 10, 24, 48, 72, and 120 h to analyse the D-glucose concentration. The D-glucose yield was calculated as g of D-glucose per 100 g of initial pretreated solid. All the enzymatic hydrolyses were performed in duplicate. 2.6. Fermentation of Enzymatic Hydrolysates The fermentation of the enzymatic hydrolysates was performed with the yeast P. tannophilus ATCC 32691 following the procedure described in Section 2.4 but with two modifications: Enzymatic hydrolysates were not detoxified, and the initial inoculum concentrations assayed were 0.5, 1.0, 1.5, and 3.0 g/dm 3 . The cultures were sampled at fixed intervals to analyse the biomass, D-glucose and ethanol concentrations. Two replicas of each fermentation were performed. 2.7. Analytical Methods The raw material as well as the solids resulting from the acid treatments and from enzymatic hydrolyses were characterized according their contents in moisture (TAPPI T257 standard), hemicellulose and cellulose [ 18 ], and insoluble acid lignin (TAPPI T222 os-74 standard). Besides, ash (TAPPI T211 standard), extractives (ASTM D 1107 84 standard), and soluble acid lignin [ 19 ] were additionally analyzed in the raw material. The Puls method [ 20 ], with a modification described elsewhere [ 16 ], was used to determine the percentage of xylans and acetyl groups in the raw material. The concentrations of D-glucose, D-xylose, L-arabinose, D-galactose and 5-hydroxymethyl-furfural in prehydrolysates, enzymatic hydrolysates and cultures were analyzed by high-performance liquid ionic chromatography (HPLIC). Processes 2020,8, 195 5 of 14 The HPLIC system (Dionex ICS 3000, Sunnyvale, CA, USA) was equipped with a CARBOPAD PA20 analytical column (3 mm × 150 mm) combined with a CARBOPAD PA20 guard column (3 mm × 30 mm), and a pulsed amperometer detector (gold electrode). Elution took place at 30 ◦ C, the eluent being 1 cm 3 /min 0.002 M NaOH. After dilution, the samples were filtered through a 0.2 µ m nylon membrane (Sartorius). Finally, ethanol, xylitol and acetic acid concentrations in liquid samples were quantified using enzymatic methods [ 21 – 23 ], using test-combination kits purchased from R-Biopharm AG (Darmstadt, Germany). All the analytical determinations were performed in duplicate. 3. Results 3.1. Dilute Acid Pretreatment: Experimental Results Table 2shows the characterization of the raw material as well as the pretreated solids and the liquid prehydrolysates obtained by dilute acid hydrolysis of the olive stones. The increase in temperature and reaction time led to a continuous decrease in the total gravimetric recovery of pretreated solid (TGR), a parameter that reached values between 56.54% and 86.53% for the most and less severe pretreatment conditions, respectively (228 ◦ C—5 min and 172 ◦ C—5 min). The loss of solid is due to the hydrolysis of different components of the raw material. Thus, hemicellulose began to depolymerize from the lowest temperature tested (172 ◦ C) and practically was removed from the pretreated solids at 200 ◦ C—5 min. Under these conditions, extractives (6.0% of the raw material) and the soluble acid lignin (2.1% of the raw material) were removed along with the hemicellulose (28.1% of the raw material), so the sum of the three fractions led to a TGR of 63.8%, theoretical value close to the average experimental value of TGR (62.78 ± 0.67%) obtained in experiments 5, 6, and 7 (Table 2). The loss of hemicellulose, extractives and acid insoluble lignin (AIL) caused the increase in the percentage of cellulose in the acid-pretreated solid, reaching a maximum of 38.62% for the experiment carried out at 200 ◦ C—9.24 min, while the highest temperatures assayed (220 ◦ C and 228 ◦ C) led to the decrease of this percentage, which would indicate a partial hydrolysis of cellulose. In relation to the AIL percentage in acid-pretreated solids, this continuously increased, from 32.01% to 48.64%, with the increase of the temperature and pretreatment time (Table 2). Table 2. Total gravimetric recovery and composition of acid-pretreated solids, and products yields (as g/100 g dry raw material) in the prehydrolysates obtained at different acid hydrolysis conditions. Acid-Treated Solids aPrehydrolysates Run Number T t TGR Hem Cel AIL Product Yield (as g/100 g Dry Raw Material) (◦C) (min) (%) (%) (%) (%) D-Xylose L-Arabinose D-Galactose D-Glucose AA 5-HMF 1 172 5 86.53 23.05 30.38 32.01 1.07 0.92 0.11 <0.01 2.47 <0.01 2 180 2 82.96 16.72 33.35 32.87 1.45 1.11 0.14 <0.01 1.45 <0.01 3 180 8 73.55 9.42 35.56 35.44 5.53 0.77 0.25 <0.01 2.71 <0.01 4 200 0.76 67.56 2.31 37.72 35.97 10.38 1.04 0.34 <0.01 3.43 <0.01 5 200 5 62.47 0.00 37.76 40.04 18.99 0.87 0.49 <0.01 3.44 <0.01 6 200 5 63.55 1.03 35.97 38.96 21.83 0.97 0.62 <0.01 3.34 <0.01 7 200 5 62.32 0.00 36.93 40.98 19.31 0.85 0.50 <0.01 3.77 <0.01 8 200 9.24 60.52 0.00 38.62 42.07 13.90 0.32 0.42 <0.01 4.12 0.03 9 220 2 58.45 0.00 33.20 44.20 8.17 0.43 0.24 0.24 4.59 0.12 10 220 8 56.78 0.00 32.02 45.87 4.57 0.21 0.23 1.46 5.77 0.80 11 228 5 56.54 0.00 25.10 48.64 2.81 0.12 0.17 1.63 5.29 1.07 a Chemical composition of 100 g of olive stones: 29.9 ± 1.1 g cellulose, 28.1 ± 1.7 g hemicellulose (of which 20.6 ± 1.1 g xylans and 4.0 ± 0.2 g acetyl groups), 27.7 ± 2.1 g acid-insoluble lignin, 2.1 ± 0.3 g acid-soluble lignin, 6.0 ± 0.3 g extractives, and 0.7 ± 0.0 g ash. TGR: total gravimetric recovery; Hem: hemicelluloses; Cel: cellulose; AIL: acid insoluble lignin; AA: acetic acid; 5-HMF: 5-hydroxymethylfurfural. Inrelation toliquidprehydrolysates, product yieldswerestronglyinfluencedbyreactionconditions. D-xylosewas themost abundantmonosaccharidein theliquid phase, reachingamaximum experimental yield of 20.04 ± 1.56 g/100 g dry raw material under the conditions of 200 ◦ C—5 min (Table 2), which represents 85.6% of the potential D-xylose in the biomass. The decrease in performance for more severe conditions would be explained by the thermal degradation of the monosaccharide. The maximum Processes 2020,8, 195 6 of 14 recoveries of L-arabinose (1.11 g/100 g dry raw material) and D-galactose (0.537 ± 0.072 g/100 g dry raw material) were achieved at low severities, while the maximum yield of D-glucose (1.63 g/100 g dry raw material) was reached at the maximum temperature assayed (228 ◦ C, run 11), in which there was an intense hydrolysis of the cellulosic fraction. Apart from carbohydrates, certain compounds that can act as inhibitors in fermentation processes, such as acetic acid and HMF, were found in the liquid prehydrolysates. The maximum yields of acetic acid (5.77 g/100 g dry raw material) and 5-HMF (1.07 g/100 g dry raw material) were achieved in the experiments carried out at the highest temperatures; in the first case, from the hydrolysis of acetyl groups of the hemicellulose and, in the second one, as a consequence of the thermal degradation of D-glucose. 3.2. Dilute Acid Pretreatment: Modelling and Optimization The application of response surface methodology (RSM) can lead to mathematical models that describe the modification of the composition of the solid residue and the liquid hydrolysate according to the studied independent variables: Temperature (X 1 ) and reaction time (X 2 ). With this aim, the mathematical principles described in Section 2.3 were applied to the experimental data (Table 2), obtaining the values included in Tables 3and 4in terms of normalized values. Data on percentages of total solids solubilization (x total solids ), hemicellulose conversion (x hemicellulose ) and cellulose conversion (x cellulose ) were obtained by relating the weight of each material removed during the acid pretreatment (total solids, hemicellulose and cellulose, respectively) to the weight of each material available in the raw material, and multiplying the result by one hundred. All equations were validated using the ANOVA test using the MODDE software. The R 2 values obtained for the seven equations varied between 0.925 and 0.999 (Tables 3and 4), indicating that the models explain between 92.5% and 99.9% of the variability contained in the responses. Table 3. Acid-pretreated solids: Estimated effects (EE), standard deviations (SD), and significance level (p) for the models representing total solids solubilisation (x total solids ), hemicellulose conversion (xhemicellulose), cellulose conversion (xcellulose), and acid insoluble lignin percentages (AIL). Response Variable Coefficient EE SD p-Value (Prob >F) R2Radjust2 xtotal solids, % Constant 37.220 ±0.290 5.460 ×10−10 0.999 0.996 X110.462 ±0.178 2.674 ×10−8 X22.630 ±0.178 2.547 ×10−5 X1·X1−4.415 ±0.212 4.673 ×10−6 X2·X2−0.667 ±0.212 2.532 ×10−2 X1·X2−1.935 ±0.251 5.891 ×10−4 xhemicellulose, % Constant 98.430 ±1.964 4.238 ×10−90.978 0.964 X121.829 ±1.653 1.166 ×10−5 X24.075 ±1.653 4.882 ×10−2 X1·X1−16.978 ±1.881 1.036 ×10−4 X1·X2−6.183 ±2.338 3.831 ×10−2 xcellulose, % Constant 22.557 ±0.770 1.051 ×10−70.994 0.990 X113.938 ±0.472 9.964 ×10−8 X22.391 ±0.472 2.289 ×10−3 X1·X14.445 ±0.562 2.159 ×10−4 X2·X2−2.565 ±0.562 3.819 ×10−3 AIL, % Constant 39.732 ±0.283 7.366 ×10−15 0.975 0.969 X15.660 ±0.332 1.411 ×10−7 X21.608 ±0.332 1.272 ×10−3 X1: temperature (in coded form), X2: time (in coded form). Significance level was defined as p<0.05. Processes 2020,8, 195 7 of 14 Table 4. Acid prehydrolysates: Estimated effects (EE), standard deviations (SD), and significance level (p) for the models representing D-xylose (Yxyl), L-arabinose (Yara) and acetic acid (YAA) yields. Factors Regression Responses EE SD p-Value (Prob >F)R2Radjust2p-Value Yxyl, % Constant 20.044 ±1.083 8.478 ×10−60.970 0.939 0.001 X11.028 ±0.663 1.820 ×10−1 X20.682 ±0.663 3.509 ×10−1 X1·X1−9.581 ±0.790 6.717 ×10−5 X2·X2−4.480 ±0.790 2.368 ×10−3 X1·X2−1.920 ±0.938 9.602 ×10−2 Yara, % Constant 0.897 ±0.048 1.512 ×10−60.967 0.945 0.000 X1−0.296 ±0.029 5.496 ×10−5 X2−0.197 ±0.029 5.294 ×10−4 X1·X1−0.181 ±0.035 2.069 ×10−3 X2·X2−0.101 ±0.035 2.791 ×10−2 YAA, % Constant 3.671 ±0.115 1.029 ×10−90.925 0.906 0.000 X11.274 ±0.135 1.321 ×10−5 X20.427 ±0.135 1.341 ×10−2 X1: temperature (in coded form), X2: time (in coded form). Significance level was defined as p<0.05. Figure 1shows the response surfaces and corresponding contour plots built from data in Tables 3 and4. In general, mathematicalmodelsshow thattotalsolids solubilization, hemicellulose, andcellulose conversions, as well as the percentage of AIL in the pretreated solids, increased with the increase in the severity of pretreatment (temperature and reaction time, Figure 1). Notwithstanding, for x total solids and, mainly, for x hemicellulose a stabilization of the conversions was observed at relatively high temperatures and reaction times. Thus, Figure 1B shows that total conversion of the hemicellulose fraction was achieved at around 200 ◦ C, the temperature exerting an effect on the response greater than that of the reaction time. In relation to the main obtained products in the acid prehydrolysate, the response surfaces for D-xylose yield (Figure 1E) and L-arabinose yield (Figure 1F) presented maximum values within the region studied. Partial differentiation of the multivariate functions Y xyl =f(X 1 ,X 2 ) and Y ara =f(X 1 , X 2 ) was carried out to determinate the values of temperature and time that provide these maximums. The predicted values were 201 ◦ C and 5.2 min, with a response corresponded to 20.1 ± 2.8 g D-xylose per 100 g dry raw material (85.9% D-xylose extraction), and 183.6 ◦ C and 2.08 min, with a response of 1.11 g L-arabinose per 100 g dry raw material. The D-xylose extraction was similar to that achieved in rice straw (80.8%) by other authors, the most suitable conditions to depolymerize xylans into xylose being 201 ◦ C, 10 min retention time and 0.5% sulfuric acid concentration [ 24 ]. Figure 1G shows that the highest temperature and reaction time assayed (220 ◦ C and 8 min, respectively) were the best conditions for acetic acid recovery. Processes 2020,8, 195 8 of 14 Processes 2020, 8, x FOR PEER REVIEW 8 of 14 Figure 1. Response surfaces and contour plots for (A) total solids solubilization, (B) hemicellulose conversion, (C) cellulose conversion, (D) acid-insoluble lignin percentage, (E) D-xylose yield, (F) L- arabinose yield, and (G) acetic acid yield as a function of reaction temperature (°C) and reaction time (min) at fixed acid concentration of 0.010 M. 3.3. Fermentation of Acid Prehydrolysates Figure 2 and Table 5 show the effect of the inoculum concentration on the fermentation of the acid prehydrolysate obtained under the conditions that maximized D-xylose recovery (201 °C—5.2 min), which was previously subjected to vacuum distillation until achieving the following composition (g/dm3): 14.45 D-xylose, 0.73 L-arabinose, 0.28 D-galactose, and 2.23 acetic acid. D- glucose was not detected at the beginning of the fermentation stage. It is worth noting that P. tannophilus yeast completely uptook both D-xylose and acetic acid, although uptake rates depended strongly on the inoculum concentration. Thus, D-xylose was almost depleted in prehydrolysates after Figure 1. Response surfaces and contour plots for ( A ) total solids solubilization, ( B ) hemicellulose conversion, ( C ) cellulose conversion, ( D ) acid-insoluble lignin percentage, ( E ) D-xylose yield, ( F ) L-arabinose yield, and ( G ) acetic acid yield as a function of reaction temperature ( ◦ C) and reaction time (min) at fixed acid concentration of 0.010 M. 3.3. Fermentation of Acid Prehydrolysates Figure 2and Table 5show the effect of the inoculum concentration on the fermentation of the acid prehydrolysate obtained under the conditions that maximized D-xylose recovery (201 ◦ C—5.2 min), which was previously subjected to vacuum distillation until achieving the following composition (g/dm 3 ): 14.45 D-xylose, 0.73 L-arabinose, 0.28 D-galactose, and 2.23 acetic acid. D-glucose was not detected at the beginning of the fermentation stage. It is worth noting that P. tannophilus yeast Processes 2020,8, 195 9 of 14 completely uptook both D-xylose and acetic acid, although uptake rates depended strongly on the inoculum concentration. Thus, D-xylose was almost depleted in prehydrolysates after 144 h and 48 h for fermentations carried out with initial biomass concentrations of 0.5 g/dm 3 and 4.0 g/dm 3 , respectively (Figure 2). In relation to acetic acid, this compound was completely uptaken within 72 h in all the fermentations. This demonstrate the low inhibition exerted by the medium on P. tannophilus, which is also evident when analyzing the biomass growth data over fermentations. In this sense, there was only a lag phase (about 10 h) in the bioreactor with the lowest initial concentration of microorganism (Figure 2A). The increase in the initial inoculum concentration from 0.5 g/dm 3 to 4.0 g/dm 3 caused a continuous decrease (from 4.8 g/dm 3 to 1.9 g/dm 3 ) in the net biomass production (Table 5). Regarding the production of ethanol and xylitol, it was observed that the latter compound was the main product of cellular metabolism except for fermentations performed with initial inoculum of 4.0 g/dm 3 . Thus, the maximum concentrations of ethanol and xylitol achieved were 0.25 g/dm 3 and 4.81 g/dm 3 , respectively, for the inoculum of 0.5 g/dm 3 , and 1.8 g/dm 3 and 1.5 g/dm 3 , respectively, for the inoculum of 4.0 g/dm 3 (Table 5). Therefore, the fermentations with the lowest inoculum concentrations were those that led to the highest yields and volumetric xylitol productivity (0.42 g/g and 0.07 g/dm 3· h), respectively), which were obtained at 72 h, while the inoculum of 4 g/dm 3 led to the highest yield of ethanol (0.17 g/g D-xylose). When comparing these results with those obtained by Saleh et al. [ 16 ] it can be pointed out that the decrease in the concentration of sulfuric acid from 0.025 M to 0.010 M in the pretreatment stage results in prehydrolysates with a lower capacity to inhibit P. tannophilus. These sugar media allow reaching, for a fixed inoculum concentration, higher concentrations of ethanol and lower biomass productions. With regards to xylitol, both prehydrolysates reached similar maximum yields (0.42 g/g in this work; 0.44 g/g in the research of Saleh et al. [16]). Processes 2020, 8, x FOR PEER REVIEW 9 of 14 144 h and 48 h for fermentations carried out with initial biomass concentrations of 0.5 g/dm3 and 4.0 g/dm3, respectively (Figure 2). In relation to acetic acid, this compound was completely uptaken within 72 h in all the fermentations. This demonstrate the low inhibition exerted by the medium on P. tannophilus, which is also evident when analyzing the biomass growth data over fermentations. In this sense, there was only a lag phase (about 10 h) in the bioreactor with the lowest initial concentration of microorganism (Figure 2A). The increase in the initial inoculum concentration from 0.5 g/dm3 to 4.0 g/dm3 caused a continuous decrease (from 4.8 g/dm3 to 1.9 g/dm3) in the net biomass production (Table 5). Regarding the production of ethanol and xylitol, it was observed that the latter compound was the main product of cellular metabolism except for fermentations performed with initial inoculum of 4.0 g/dm3. Thus, the maximum concentrations of ethanol and xylitol achieved were 0.25 g/dm3 and 4.81 g/dm3, respectively, for the inoculum of 0.5 g/dm3, and 1.8 g/dm3 and 1.5 g/dm3, respectively, for the inoculum of 4.0 g/dm3 (Table 5). Therefore, the fermentations with the lowest inoculum concentrations were those that led to the highest yields and volumetric xylitol productivity (0.42 g/g and 0.07 g/dm3·h), respectively), which were obtained at 72 h, while the inoculum of 4 g/dm3 led to the highest yield of ethanol (0.17 g/g D-xylose). When comparing these results with those obtained by Saleh et al. [16] it can be pointed out that the decrease in the concentration of sulfuric acid from 0.025 M to 0.010 M in the pretreatment stage results in prehydrolysates with a lower capacity to inhibit P. tannophilus. These sugar media allow reaching, for a fixed inoculum concentration, higher concentrations of ethanol and lower biomass productions. With regards to xylitol, both prehydrolysates reached similar maximum yields (0.42 g/g in this work; 0.44 g/g in the research of Saleh et al. [16]). Figure 2. Acid prehydrolysate: Effect of inoculum concentration (A, 0.5 g/dm3; B, 1.0 g/dm3; C, 2.0 g/dm3; D, 4.0 g/dm3) on D-xylose () and acetic acid () consumption, and biomass () and xylitol () production by P. tannophilus at 30 °C and pH 4.5. 4.81 0 3 6 9 12 15 18 0 50 100 150 Concentration (g/dm3) Time (h) (A) 3.25 0 3 6 9 12 15 18 0 50 100 150 Concentration (g/dm3) Time (h) (B) 2.43 0 3 6 9 12 15 18 0 50 100 150 Concentration (g/dm3) Time (h) (C) 1.50 0 3 6 9 12 15 18 0 50 100 150 Concentration (g/dm3) Time (h) (D) Figure 2. Acid prehydrolysate: Effect of inoculum concentration (( A ), 0.5 g/dm 3 ; ( B ), 1.0 g/dm 3 ; ( C ), 2.0 g/dm 3 ; ( D ), 4.0 g/dm 3 ) on D-xylose ( • ) and acetic acid (  ) consumption, and biomass (  ) and xylitol (N) production by P. tannophilus at 30 ◦C and pH 4.5.